12,468 results

1. px335 Slc1a3 nickase 1

   Plasmid

   #129410

   Purpose

   Cas9n (nickase) with sgRNA directed to the terminal exon of mouse Slc1a3 gene

   Depositor

   Walker Jackson

   Article

   Kaczmarczyk et al Sci Rep. 2021 Mar 8;11(1):5412. doi: 10.

   Insert

   sgRNA directing Cas9n to the proximity of Slc1a3 STOP codon (Slc1a3 Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. px335 Slc1a3 nickase 2

   Plasmid

   #129411

   Purpose

   Cas9n (nickase) with sgRNA directed to the terminal exon of mouse Slc1a3 gene

   Depositor

   Walker Jackson

   Article

   Kaczmarczyk et al Sci Rep. 2021 Mar 8;11(1):5412. doi: 10.

   Insert

   sgRNA targeting Slc1a3 (Slc1a3 Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLeGO.sgGata1.4.RUNX1A.iG2

   Plasmid

   #181976

   Purpose

   gene knock out of mouse GATA1, overexpression of 3xFLAG-RUNX1A (human)

   Depositor

   Jan-Henning Klusmann

   Inserts

   GATA1 gRNA: CCTAGACCAGGAAAATCCAT (Gata1 Mouse)
   RUNX1A (RUNX1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pORANGE Tubb3-2xGFP KI

   Plasmid

   #183443

   Purpose

   GFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   gRNA and 2xGFP donor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pOC5 smFP-HA-Cacna1e KI

   Plasmid

   #183441

   Purpose

   FlpOFF knock-in for smFP-HA-CaV2.3 (amino acid position: G5)

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   gRNA and smFP HA donor
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pOC2 Gria1-Halo KI

   Plasmid

   #183433

   Purpose

   CreOFF knock-in for GluA1-Halo (amino acid position: stop codon)

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   gRNA and Halo donor
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pORANGE Tubb3-3xGFP KI

   Plasmid

   #183444

   Purpose

   GFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   gRNA and 3xGFP donor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pOC2 Tubb3 no donor

   Plasmid

   #183431

   Purpose

   CreOFF gRNA expression for beta3-tubulin (amino acid position: stop codon)

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pOC4 Grin1-smFP-V5 KI

   Plasmid

   #183439

   Purpose

   FlpON knock-in for GluN1-smFP-V5 (amino acid position: A20)

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   gRNA and smFP V5 donor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pOC2 Gria1-GFP KI

    Plasmid

    #183430

    Purpose

    CreOFF knock-in for GluA1-GFP (amino acid position: stop codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and GFP donor
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPHR-mCherry-RGG-GFP-RGG-mHP1alpha

    Plasmid

    #181893

    Purpose

    Soluble light-dependent effector protein (HP1alpha fused to the arginine/glycine-rich RGG domain of LAF-1) for optodroplet formation or recruitment to CIBN localizers

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PHR-mCherry-RGG-GFP-RGG-mHP1alpha (Cbx5 Mustard Weed, Mouse)
    

    Use

    Tags

    PHR-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGFP-nE-mHP1alpha-delCTE

    Plasmid

    #181896

    Purpose

    Fluorescently tagged HP1alpha, serines in N-terminal extension (NTE) replaced by glutamates, C-terminal deletion

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    nE-mHP1alpha-delCTE (Cbx5 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    S11E, S12E, S13E, S14E, 14 aa C-terminal deletion

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPHR-mCherry-PGL-1-mHP1alpha

    Plasmid

    #181894

    Purpose

    Soluble light-dependent effector protein (HP1alpha fused to PGL-1) for optodroplet formation or recruitment to CIBN localizers

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PHR-mCherry-PGL-1-mHP1alpha (Cbx5 Mustard Weed, Mouse)
    

    Use

    Tags

    PHR-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPHR-mCherry-nE-mHP1alpha-delCTE

    Plasmid

    #181891

    Purpose

    Soluble light-dependent effector protein (HP1alpha, phosphomimetic NTE mutant with deletion of C-terminal extension)for optodroplet formation or recruitment to CIBN localizers

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PHR-mCherry-nE-mHP1alpha-delCTE (Cbx5 Mustard Weed, Mouse)
    

    Use

    Tags

    PHR-mCherry

    Expression

    Mammalian

    Mutation

    S11E, S12E, S13E, S14E, 14 aa C-terminal deletion

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGFP-FUSN-mHP1alpha

    Plasmid

    #181897

    Purpose

    Fluorescently tagged HP1alpha fused to the N-terminal domain of the fused in sarcoma protein (FUSN)

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    FUSN-mHP1alpha (Cbx5 Mouse, Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPHR-mCherry-nE-mHP1alpha

    Plasmid

    #181890

    Purpose

    Soluble light-dependent effector protein (HP1alpha, phosphomimetic NTE mutant) for optodroplet formation or recruitment to CIBN localizers

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PHR-mCherry-nE-mHP1alpha (Cbx5 Mustard Weed, Mouse)
    

    Use

    Tags

    PHR-mCherry

    Expression

    Mammalian

    Mutation

    S11E, S12E, S13E, S14E

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGFP-PGL-1-mHP1alpha

    Plasmid

    #181899

    Purpose

    Fluorescently tagged HP1alpha fused to PGL-1

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PGL-1-mHP1alpha (Cbx5 Nematode, Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMECP2-RFP

    Plasmid

    #181905

    Purpose

    Fluorescently tagged MECP2, RFP

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    MECP2 (Mecp2 Mouse)
    

    Use

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRGG-GFP-RGG-mHP1alpha

    Plasmid

    #181898

    Purpose

    Fluorescently tagged HP1alpha fused to the arginine/glycine-rich RGG domain of LAF-1

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    RGG-GFP-RGG (Cbx5 Nematode, Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Rcan1_pLENTI-CAG-IRES-GFP

    Plasmid

    #177000

    Purpose

    Mammalian lentiviral expression vector encoding Rcan1

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    Rcan1 (Rcan1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Rcan1_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176978

    Purpose

    Mammalian expression vector encoding Rcan1 and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    Rcan1 (Rcan1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTRE3G-ZSGreen1-mHP1alpha

    Plasmid

    #181913

    Purpose

    Inducible mammalian expression vector for ZsGreen1-tagged HP1alpha

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    mHP1alpha (Cbx5 Mouse)
    

    Use

    Tags

    ZSGreen1

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Lenti-sgArhgap35#2/Cre

    Plasmid

    #173570

    Purpose

    Expresses a Arhgap35-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Arhgap35 (Grlf1 Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Lenti-sgArhgap35#1/Cre

    Plasmid

    #173569

    Purpose

    Expresses a Arhgap35-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Arhgap35 (Grlf1 Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Gucy1b2-IRES-nlsCre-IRES-taulacZ-FNF TV

    Plasmid

    #105196

    Purpose

    targeting vector to generate a Gucy1b2-IRES-nlsCre-IRES-taulacZ mouse strain.

    Depositor

    Peter Mombaerts

    Article

    Koike et al Neuron. 2021 Aug 4;109(15):2469-2484.e7.

    Insert

    Gucy1b2-IRES-nlsCre-IRES-taulacZ-FNF (Gucy1b2 Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

26. pcDNA6-5'UTR(mtIF3)-Dendra2-3'UTR(mtIF3)

    Plasmid

    #182369

    Purpose

    Dendra2-based translation reporter

    Depositor

    Chunghun Lim

    Article

    Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

    Insert

    mtIF3 (Mtif3 Mouse)
    

    Use

    Tags

    Pal-Dendra2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. psiCheck2-SV40p-mtIF3(SR)-3xFLAG-HSVTKp-mCherry

    Plasmid

    #182381

    Purpose

    Dual expression construct encoding shRNA-resistant mtIF3 and mCherry from separate promoters

    Depositor

    Chunghun Lim

    Article

    Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

    Inserts

    mtIF3 (Mtif3 Mouse)
    mCherry
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Changed CDS sequence from 'ccacgttcaagtcacga…

    Promoter

    HSV TK promoter and SV40 promoter

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. psiCheck2-SV40p-mtIF3(SR)-3xFLAG-CMVp-mito-mTFP1

    Plasmid

    #182380

    Purpose

    Dual expression construct encoding shRNA-resistant mtIF3 and mito-mTFP1 from separate promoters

    Depositor

    Chunghun Lim

    Article

    Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

    Inserts

    mtIF3 (Mtif3 Mouse)
    mito-mTFP1
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Changed CDS sequence from 'ccacgttcaagtcacga…

    Promoter

    CMV promoter and SV40 promoter

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MSCV-mouse Mdm2 mutant M3 (399-489)-IRES-GFP

    Plasmid

    #180490

    Purpose

    Role of Mdm2 variants in tumorigenesis

    Depositor

    Martine Roussel

    Article

    Dang et al Cancer Res. 2002 Feb 15;62(4):1222-30.

    Inserts

    Mouse Mdm2 mutant
    Mouse Mdm2 mutant
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    399-489

    Promoter

    MSCV

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MSCV-mouse Mdm2 mutant M2 (299-489)-IRES-GFP

    Plasmid

    #180489

    Purpose

    Role of Mdm2 variants in tumorigenesis

    Depositor

    Martine Roussel

    Article

    Dang et al Cancer Res. 2002 Feb 15;62(4):1222-30.

    Insert

    Mouse Mdm2 mutant
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    299-489

    Promoter

    MSCV

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. psiCheck2-SV40p-5'UTR-mtIF3(SR)-3xFLAG-3'UTR-CMVp-mito-mTFP1

    Plasmid

    #182378

    Purpose

    Dual expression construct encoding shRNA-resistant mtIF3 and mito-mTFP1 from separate promoters

    Depositor

    Chunghun Lim

    Article

    Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

    Inserts

    mtIF3 (Mtif3 Mouse)
    mito-mTFP1
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Changed CDS sequence from 'ccacgttcaagtcacga…

    Promoter

    CMV promoter and SV40 promoter

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. psiCheck2-SV40p-5'UTR-mtIF3(SR)-3xFLAG-3'UTR-HSVTKp-mCherry

    Plasmid

    #182379

    Purpose

    Dual expression construct encoding shRNA-resistant mtIF3 and mCherry from separate promoters

    Depositor

    Chunghun Lim

    Article

    Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

    Inserts

    mtIF3 (Mtif3 Mouse)
    mCherry
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Changed CDS sequence from 'ccacgttcaagtcacga…

    Promoter

    HSV TK promoter and SV40 promoter

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA6-mtIF3-Dendra2

    Plasmid

    #182371

    Purpose

    Dendra2-based translation reporter

    Depositor

    Chunghun Lim

    Article

    Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

    Insert

    mtIF3 (Mtif3 Mouse)
    

    Use

    Tags

    Pal-Dendra2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. PX552-mScn8a-3xHA-Syn-smFP-flag

    Plasmid

    #182563

    Purpose

    For mouse Nav1.6 knockin with 3xHA at the C-terminal

    Depositor

    James Zhe Liu

    Article

    Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

    Inserts

    mouse Scn8a gRNA and 3xHA donor DNA
    smFP-flag
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    EF1 and U6

    Available since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. PX552-mScn8a-HaloTag-V5-EF1-sfGFP

    Plasmid

    #182565

    Purpose

    For mouse Nav1.6 knockin with HaloTag-V5 at the C-terminal

    Depositor

    James Zhe Liu

    Article

    Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

    Inserts

    mouse Scn8a gRNA and HaloTag-V5 donor DNA
    sfGFP
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    EF1 and U6

    Available since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. PX552-mScn8a-3xV5-EF1-smFP-flag

    Plasmid

    #182562

    Purpose

    For mouse Nav1.6 knockin with 3xV5 at the C-terminal

    Depositor

    James Zhe Liu

    Article

    Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

    Inserts

    mouse Scn8a gRNA and 3xV5 donor DNA
    smFP-flag
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    EF1 and U6

    Available since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Rufy3 scFv [N483/126]

    Plasmid

    #182089

    Purpose

    Mammalian Expression of Rufy3 scFv. Derived from hybridoma N483/126.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targeting Rufy3 (Mus musculus) (Rufy3 Mouse)
    

    Use

    Tags

    Sortase, His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Arl13b scFv [N295B/66]

    Plasmid

    #182086

    Purpose

    Mammalian Expression of Arl13b scFv. Derived from hybridoma N295B/66.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targeting Arl13b (Mus musculus) (Arl13b Mouse)
    

    Use

    Tags

    HA, His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. RGS14 scFv [N133/21]

    Plasmid

    #182081

    Purpose

    Mammalian Expression of RGS14 scFv. Derived from hybridoma N133/21.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targeting RGS14 (Rattus norvegicus) (Rgs14 Mouse)
    

    Use

    Tags

    Sortase, His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Kv1.2 K+ channel scFv [K14/16]

    Plasmid

    #182064

    Purpose

    Mammalian Expression of Kv1.2 K+ channel scFv. Derived from hybridoma K14/16.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targeting Kv1.2 K+ channel (Homo sapiens) (KCNA2 Mouse)
    

    Use

    Tags

    HA, His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. Parvalbumin scFv [L114/81]

    Plasmid

    #182073

    Purpose

    Mammalian Expression of Parvalbumin scFv. Derived from hybridoma L114/81.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targeting Parvalbumin (Rattus norvegicus) (Pvalb Mouse)
    

    Use

    Tags

    HA, His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. Kv2.1 K+ channel scFv [K89/34]

    Plasmid

    #182069

    Purpose

    Mammalian Expression of Kv2.1 K+ channel scFv. Derived from hybridoma K89/34.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targeting Kv2.1 K+ channel (Rattus norvegicus) (Kcnb1 Mouse)
    

    Use

    Tags

    HA, His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pX458_sgAgo2_Nterm

    Plasmid

    #170920

    Purpose

    Expresses spCas9 and sgRNA targeting the N-terminus of Ago2 for N-terminal tagging

    Depositor

    Constance Ciaudo

    Article

    Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

    Insert

    sgAgo2 (Ago2 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pX458_sgAgo1_Nterm

    Plasmid

    #170921

    Purpose

    Expresses spCas9 and sgRNA targeting the N-terminus of Ago1 for N-terminal tagging

    Depositor

    Constance Ciaudo

    Article

    Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

    Insert

    sgAgo1 (Ago1 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pUC19_HR-BirA-FLAG_Ago2

    Plasmid

    #170918

    Purpose

    Encodes BirA-FLAG with left and right homology arms for endogenous tagging of Ago2

    Depositor

    Constance Ciaudo

    Article

    Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

    Insert

    BirA-FLAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    lac

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pUC19_HR-BirA-FLAG_Ago1

    Plasmid

    #170919

    Purpose

    Encodes BirA-FLAG with left and right homology arms for endogenous tagging of Ago1

    Depositor

    Constance Ciaudo

    Article

    Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

    Insert

    BirA-FLAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    lac

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pX458_sgRNA_Ago1_5

    Plasmid

    #172470

    Purpose

    Expresses SpCas9, GFP, and sgRNA targeting Ago1

    Depositor

    Constance Ciaudo

    Article

    Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

    Insert

    sgAgo1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pX458_sgRNA_Ago1_6

    Plasmid

    #172471

    Purpose

    Expresses SpCas9, GFP, and sgRNA targeting Ago1.

    Depositor

    Constance Ciaudo

    Article

    Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

    Insert

    sgAgo1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. AiP949-Slc17a7-IRES2-FlpO-WPRE-neo

    Plasmid

    #181776

    Purpose

    targeting vector

    Depositor

    Tanya Daigle

    Insert

    Slc17a7 homology arms, FlpO
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pCAG1.1-Armc12 delta ARM/FLAG

    Plasmid

    #180496

    Purpose

    Expression vector of ARM domain deleted mouse Armc12 tagged with FLAG at C-terminus. See Fig.4D of Shimada, K. et al. Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021.

    Depositor

    Masahito Ikawa

    Article

    Shimada et al Proc Natl Acad Sci U S A. 2021 Feb 9;118

    Insert

    armadillo repeat containing 12 (Armc12 Mouse)
    

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    ARM domain of Armc12 is deleted

    Promoter

    CAG promoter

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge