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Showing: 4181 - 4200 of 17814 results

  6. hEF1α-H2B-mVenus-IRES-mCherry PGK-Puromycin

    Plasmid
    #99278
    Purpose
    Dual fluorescent reporter - constitutive expression of mVenus (515/528) localized in the nucleus, mCherry (587/610) untargeted and visualized in the cytoplasm, and Puromycin resistance
    Depositor
    Jordan Miller
    Article
    Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/
    Inserts
    H2B-mVenus-IRES-mCherry
    Puro
    Use
    Lentiviral
    Tags
    H2B, IRES, PGK, mCherry, and mVenus
    Promoter
    EF1 alpha-human and PGK-mouse
    Available Since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99278-plasmid-map-sequence-id-262659

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  7. G protein alpha-s(alpha3beta5/G226A/A366S)

    Plasmid
    #55797
    Purpose
    This highly effective dominant negative G protein alpha-s mutant contains, in addition to the mutations in alpha-s(alpha3beta5/G226A), the A366S mutation, which increases GDP release.
    Depositor
    Catherine Berlot
    Article
    Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.
    Insert
    alpha-s (alpha3beta5/G226A/A366S) (Gnas Rat)
    Tags
    internal EE epitope (residues 189-194 in alpha-s …
    Expression
    Mammalian
    Mutation
    N271K, K274D, R280K, T284D, I285T, G226A, A366S i…
    Promoter
    CMV
    Available Since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    55797-plasmid-map-sequence-id-112181

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  9. pDule-3-nitroTyrosine (5B)

    Plasmid
    #85498
    Purpose
    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.
    Insert
    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32H H70C D158S I159A L162R
    Promoter
    lpp (constitutive)
    Available Since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85498-plasmid-map-sequence-id-169862

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  10. PM2-FRB-CFP

    Plasmid
    #67518
    Purpose
    FRB conjugated plasma membrane targeting sequences 2 from Lyn11(GCIKSKGKDSA)
    Depositor
    Tamas Balla
    Article
    Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.
    Insert
    mTOR (MTOR Human)
    Tags
    CFP, HA, and PM tethering sequence from human Lyn…
    Expression
    Mammalian
    Mutation
    FRB domain only (residues 2019–2114)
    Promoter
    CMV
    Available Since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. pLenti6.2_mCherry_roGFP2_Orp1

    Plasmid
    #155043
    Purpose
    Fluorescent redox reporter
    Depositor
    Vanessa LaPointe
    Insert
    Orp1 (HYR1 Budding Yeast)
    Use
    Lentiviral
    Tags
    roGFP2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    155043-plasmid-map-sequence-id-311571

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  17. slc1a3b:myrGCaMP6s-P2A-H2AmCherry

    Plasmid
    #170209
    Purpose
    Drives myrGCaMP6s-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGCaMP6s calcium indicator expressed in the membrane and mCherry labels the nucleus
    Depositor
    Kelly Monk
    Article
    Chen et al Nat Neurosci. 2020 Oct;23(10):1297-1306.
    Insert
    slc1a3b (slc1a3b Zebrafish)
    Use
    Zebrafish
    Tags
    myrGCaMP6s-P2A-H2AmCherry
    Promoter
    slc1a3b
    Available Since
    June 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    170209-plasmid-map-sequence-id-333268

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  18. pMM178

    Plasmid
    #61270
    Purpose
    Also referred to as pRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains a pBBR1 origin and chloramphenicol resistance.
    Depositor
    Timothy Lu
    Article
    Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103
    Insert
    Cas9, tracrRNA, crRNA
    Use
    CRISPR
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    pLtetO, native, native (respectively)
    Available Since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    61270-plasmid-map-sequence-id-99577

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  20. pTO_CHMP3_LAP

    Plasmid
    #101849
    Purpose
    Tet-inducible expression of human CHMP3 with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration
    Depositor
    Daniel Gerlich
    Article
    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d
    Insert
    Charged Multivesicular Body Protein 3 (CHMP3 Human)
    Use
    Gateway cloning, flp-frt recombination, tet-induc…
    Tags
    Localization and Affinity Purification (LAP) tag
    Expression
    Bacterial and Mammalian
    Promoter
    CMV
    Available Since
    Dec. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    101849-plasmid-map-sequence-id-200249

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Showing: 4181 - 4200 of 17814 results