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pICH86966

Plasmid

#48075

Depositor

Sylvestre Marillonnet

Publication

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available since

Nov. 25, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

5 Articles

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pH2rU3_ForInd_Omicron_sinobiological_Spiked21_T7_CMV_ZsGT2APurR

Plasmid

#204146

Purpose

Lentivirus backbone with BA.1 spike under an inducible promoter and constitutive expressed zsGreen-T2A-PuR genes

Depositor

Jesse Bloom

Publication

Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

Inserts

SARS-CoV-2 BA.1 variant spike
zsGreen-T2A-PuR

Use

Lentiviral

Expression

Mammalian

Mutation

21 amino acid cytoplasmic tail deletion

Promoter

CMV and TRE3G

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA5/FRT/TO_H2B-HaloTag7-T2A-Tom20-HaloTag9

Plasmid

#175547

Purpose

Co-expression of HaloTag7 (nuclear) and 9 (mitochondrial surface) in mammalian cells

Depositor

Kai Johnsson

Publication

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

H2B-HaloTag7 and Tomm20-HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac StrepII msfGFP TEV cloning vector with BioBrick polycistronic restriction sites(11R-GFP)

Plasmid

#48297

Depositor

Scott Gradia

Publication

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

StrepII and msfGFP

Expression

Insect

Available since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pSO221 Phis-72 BirA GFP

Plasmid

#45100

Depositor

Steven Henikoff

Publication

Steiner et al Genome Res. 2012 Apr;22(4):766-77. doi:

Inserts

BirA::GFP
unc-119 (unc-119 Nematode)

Tags

GFP

Expression

Worm

Promoter

his-72 and unc-119

Available since

June 4, 2013

Availability

Academic Institutions and Nonprofits only

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TRUPATH Triple Ga13

Plasmid

#196062

Purpose

Encodes a G alpha subunit (GNA13) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

Depositor

Justin English

Publication

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Inserts

GAlpha13-RLuc8 (GNA13 Human)
GGamma9-GFP2
GBeta3 (GNB3 Human)

Use

Luciferase

Tags

GFP2 and GSAG linker

Expression

Mammalian

Mutation

RLuc8 and flanking SGGGGS linkers have been inser…

Promoter

CMV

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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LeGO-iV

Plasmid

#27360

Depositor

Boris Fehse

Publication

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

U6 promoter, SFFV promoter, IRES-Venus

Use

Lentiviral

Expression

Mammalian

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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CMV AP-4

Plasmid

#12101

Depositor

Robert Tjian

Publication

Hu et al Genes Dev. 1990 Oct . 4(10):1741-52.

Insert

AP-4 (TFAP4 Human)

Expression

Mammalian

Available since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pLenti_CMV:Flag-NLuc_SV40:PuroR

Plasmid

#183045

Purpose

Expresses FLAG-tagged NanoLuciferase (NLuc-only) in mammalian cells.

Depositor

Antonio Amelio

Publication

Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

Insert

NanoLuciferase

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pWUKI 1+2

Plasmid

#79879

Purpose

Encodes Cas1+2 and a CRISPR array

Depositor

George Church

Publication

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1
Cas2
CRISPR Array

Expression

Bacterial

Promoter

T7

Available since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pLH-stsgRNA2.1

Plasmid

#64117

Purpose

Vector for expression of St1 sgRNA2.1 in mammalian cells

Depositor

Thoru Pederson

Publication

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 sgRNA2.1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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PB-TRE-OKSM

Plasmid

#204498

Purpose

Expresses Oct4, Klf4, Sox2 and c-Myc by tet-on promoter in mammalian cells

Depositor

Wataru Fujii

Publication

Matsuya et al Sci Rep. 2024 Feb 8;14(1):3204. doi: 10.

Inserts

OCT4 (POU5F1 Human)
KLF4 (KLF4 Human)
SOX2 (SOX2 Human)

Expression

Mammalian

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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Bxb1-surrogate-reporter

Plasmid

#65624

Purpose

Fluorescent reporter to monitor the activty of Bxb1; If Bxb1 and attB donor plasmid containing GFP is present in the cell, recombination of the donor into the reporter leads to expression of GFP.

Depositor

Sebastian Bultmann

Publication

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attP

Use

Bxb1

Expression

Mammalian

Available since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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FCK-QuasAr2-Citrine

Plasmid

#59172

Purpose

Fluorescent membrane voltage sensor

Depositor

Adam Cohen

Publication

Zou et al Nat Commun. 2014 Aug 13;5:4625. doi: 10.

Insert

QuasAr2-Citrine eFRET

Use

Lentiviral

Expression

Mammalian

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCMV-G-Rac1

Plasmid

#180575

Purpose

ddGFP sensor of Rac1

Depositor

Won Do Heo

Publication

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddGFP sensor of Rac1

Expression

Mammalian

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-R-Rac1

Plasmid

#180576

Purpose

ddRFP sensor of Rac1

Depositor

Won Do Heo

Publication

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddRFP sensor of Rac1

Expression

Mammalian

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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Hygro-iDEST

Plasmid

#75339

Purpose

GATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expression

Depositor

Danwei Huangfu

Publication

Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

Insert

TRE-DEST

Expression

Mammalian

Promoter

TRE-tight

Available since

May 24, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-DUET-GST-eIF4G1-His6, eIF4E

Plasmid

#37232

Depositor

Jon Lorsch

Publication

Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

Inserts

TIF4631 (TIF4631 Budding Yeast)
CDC33 (CDC33 Budding Yeast)

Tags

GST and His6

Expression

Bacterial

Promoter

T7

Available since

Sept. 4, 2012

Availability

Academic Institutions and Nonprofits only
pUC57-CmYLCV

Plasmid

#205245

Purpose

For plant prime editing in wheat plants or monocotyledons protoplasts

Depositor

Zong Yuan

Publication

Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

Insert

CmYLCV-csy4_binding_site-esgRNA-tevopreQ1-csy4_binding_site

Use

CRISPR

Expression

Plant

Promoter

CmYLCV

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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Geminin-mStrawberry reporter

Plasmid

#158690

Purpose

This plasmid is a Geminin cell cycle reporter. It has CMV promoter driving the expression of Geminin fused to mStrawberry-pGK-BSD

Depositor

Ravid Straussman

Publication

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mStrawberry

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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