We narrowed to 9,202 results for: sacs

1. pfa6a mScarlet-TsCC(B)::KANMX

   Plasmid

   #176397

   Purpose

   Plasmid for cloning mScarlet fused to TsCC(B) coiled coil into yeast.

   Depositor

   Matthew Good

   Article

   Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

   Insert

   mScarlet-TsCC(B)
   

   Use

   Tags

   mScarlet fused to TsCC(B)

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   none

   Available since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-FLEX-EGFP-KASH

   Plasmid

   #154373

   Purpose

   Vector for Cre-dependent expression of EGFP tagged KASH protein domain

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   EGFP-KASH
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGal4x5-E4T-G210

   Plasmid

   #171077

   Purpose

   The resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp construct for ampilifying the immobilized template for assays. Originally came from Michael Carey, was submitted with his permission

   Depositor

   Joan Conaway

   Article

   He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

   Insert

   Gal4-E4-Gless-cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMK419 (GAL-OsTIR1(F74G))

   Plasmid

   #140656

   Purpose

   GAL-OsTIR1(F74G)

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   GAL-OsTIR1(F74G)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEasyCas9

   Plasmid

   #184909

   Purpose

   Expresses the Streptococcus pyogenes Cas9 protein

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHLUM

   Plasmid

   #40276

   Depositor

   Markus Ralser

   Article

   Mulleder et al Nat Biotechnol. 2012 Dec 7;30(12):1176-8

   Inserts

   LEU2 (LEU2 Budding Yeast)
   URA3 (URA3 Budding Yeast)
   MET15/17 (MET17 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   endogenous LEU2, endogenous MET15/17, and endogen…

   Available since

   Nov. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRCC-K

   Plasmid

   #81191

   Purpose

   Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance

   Depositor

   Eckhard Boles

   Article

   Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

   Inserts

   Cas9
   empty gRNA cassette
   

   Use

   CRISPR

   Tags

   NTS

   Expression

   Yeast

   Mutation

   Please see depositor comments below. and WT - cod…

   Promoter

   ROX3 and SNP52p

   Available since

   Sept. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pR26 CAG/GFP Dest

   Plasmid

   #74281

   Purpose

   Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, GFP, for gateway cloning

   Depositor

   Ralf Kuehn

   Article

   Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

   Insert

   Rosa26 5-homology region
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. gRNA-ura-HYB

   Plasmid

   #64330

   Purpose

   ura3 deletion gRNA cassette carried by pRS42H

   Depositor

   Yong-Su Jin

   Article

   Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

   Insert

   gBlock product of ura3 deletion gRNA cassette
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

10. LV-TRE-VP64 human MyoD-T2A-dsRedExpress2

    Plasmid

    #60629

    Purpose

    Expresses VP64 human MyoD and dsRedExpress2 in response to doxycycline

    Depositor

    Charles Gersbach

    Article

    Kabadi et al ACS Synth Biol. 2014 Dec 10.

    Insert

    VP64 human MyoD (MYOD1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pR26 CAG/GFP Asc

    Plasmid

    #74285

    Purpose

    Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette and GFP, for cloning into AscI

    Depositor

    Ralf Kuehn

    Article

    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

    Insert

    Rosa26 5-homology region
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)

    Plasmid

    #78231

    Purpose

    Expresses Cas9 protein, under the control of the TEF1 promoter, and CYC terminator. KanMX resistance

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pK036.TRE-Flpe-WPRE (Supernova)

    Plasmid

    #85007

    Purpose

    For Flpe/FRT-based Supernova, pK036 should be used with pK068 etc.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    NLS-FLPe-WPRE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSJ1256 (pFA6-link-yGFP1-10-CaURA3MX)

    Plasmid

    #86419

    Purpose

    C-terminal PCR tagging module for yeast codon optimized split GFP1-10

    Depositor

    Sue Jaspersen

    Article

    Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

    Insert

    GFP1-10
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pILGFP91

    Plasmid

    #83548

    Purpose

    Used to evaluate the expression output of PGK1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    PGK1 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PGK1

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSJ1321 (pRS315-NOP1pr-GFP11-mCherry-PUS1)

    Plasmid

    #86413

    Purpose

    nuclear split GFP11 reporter with mCherry

    Depositor

    Sue Jaspersen

    Article

    Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

    Insert

    Nop1pr-GFP11-mCherry-PUS1
    

    Use

    Tags

    GFP11 and mCherry

    Expression

    Yeast

    Mutation

    Promoter

    NOP1

    Available since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSJ1568 (pRS315-NOP1pr-GFP11-mCherry-SCS2TM)

    Plasmid

    #86416

    Purpose

    ONM/ER split GFP11 reporter with mCherry

    Depositor

    Sue Jaspersen

    Article

    Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

    Insert

    NOP1pr-GFP11-mCherry-Scs2TM
    

    Use

    Tags

    GFP11 and mCherry

    Expression

    Yeast

    Mutation

    Promoter

    NOP1

    Available since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSJ1602 (pRS315-NOP1pr-mCherry-SCS2TM-GFP11)

    Plasmid

    #86417

    Purpose

    luminal split GFP11 reporter with mCherry

    Depositor

    Sue Jaspersen

    Article

    Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

    Insert

    NOP1pr-mCherry-SCS2TM-GFP11
    

    Use

    Tags

    GFP11 and mCherry

    Expression

    Yeast

    Mutation

    Promoter

    NOP1

    Available since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSJ1679 (pRS315-NOP1pr-GFP11-PUS1)

    Plasmid

    #86414

    Purpose

    nuclear split GFP11 reporter without mCherry

    Depositor

    Sue Jaspersen

    Article

    Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

    Insert

    NOP1pr-GFP11-PUS1
    

    Use

    Tags

    GFP11 and mCherry

    Expression

    Yeast

    Mutation

    Promoter

    NOP1pr

    Available since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3 p53 WT

    Plasmid

    #69003

    Purpose

    mammalian expression of wild type human p53

    Depositor

    David Meek

    Article

    Loughery et al Nucleic Acids Res. 2014 Jul;42(12):7666-

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCRCT

    Plasmid

    #60621

    Purpose

    Plasmid encoding iCas9, tracrRNA and crRNAs

    Depositor

    Huimin Zhao

    Article

    Bao et al ACS Synth Biol. 2014 Sep 19.

    Inserts

    iCas9
    tracrRNA
    

    Use

    CRISPR

    Tags

    FLAG and SV40 NLS

    Expression

    Yeast

    Mutation

    changed Aspartate 147 to Tyrosine, Proline 411 to…

    Promoter

    RPR1p and TEF1p

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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22. iRFP-Sec61beta

    Plasmid

    #108125

    Purpose

    ER marker

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    iRFP:SEC61B (SEC61B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDP0437

    Plasmid

    #221127

    Purpose

    Plasmid for generating integration cassette to C-terminally tag gene of interest with pHluorin::HPHMX

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    pHluorin
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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24. HDAC5 Flag

    Plasmid

    #13822

    Purpose

    Mammalian expression of human histone deacetylase 5 with flag tag

    Depositor

    Eric Verdin

    Article

    Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

    Insert

    HDAC5 (HDAC5 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. mCherry-C1-ORP5-H478-9A-deltaTMD-FKBP

    Plasmid

    #220075

    Purpose

    expresses a mutant ORP5 that is unable to bind to PI4P with an mCherry fluorophore and an FKBP

    Depositor

    Gerry Hammond

    Article

    Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

    Insert

    OSBPL5 H478/9A (OSBPL5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H478/9A, deltaTMD

    Promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. mCherry-C1-ORP5deltaORDdeltaTMD-FKBP

    Plasmid

    #220074

    Purpose

    expresses ORP5 with the OSBP-related ligand-binding domain and the transmembrane domain deleted. The mCherry fluorophore and FKBP are attached to ORP5.

    Depositor

    Gerry Hammond

    Article

    Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

    Insert

    OSBPL5deltaORDdeltaTMD (OSBPL5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deltaORDdeltaTMD

    Promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGL2-NOS2Promoter-Luciferase

    Plasmid

    #19296

    Depositor

    Charles Lowenstein

    Article

    Lowenstein et al Proc Natl Acad Sci U S A. 1993 Oct 15. 9

    Insert

    NOS2 Promoter (Nos2 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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28. ampl43

    Plasmid

    #161830

    Purpose

    pRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.

    Depositor

    Saeed Tavazoie

    Article

    Momen-Roknabadi et al Commun Biol. 2020 Nov 27;3(1):723. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. ISceI-GR-RFP

    Plasmid

    #17654

    Depositor

    Tom Misteli

    Article

    Soutoglou et al Nat Cell Biol. 2007 Jun . 9(6):675-82.

    Insert

    I-SceI-GRLBD (SCEI, Nr3c1 Rat, Budding Yeast)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    C655G mutation in the glucocorticoid receptor (se…

    Promoter

    Available since

    March 28, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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30. pPB-CAG-rtTA-IRES-Hygro

    Plasmid

    #102423

    Purpose

    Mammalian expression vector for tetracycline-transactivator (rtTA), IRES-Hygro

    Depositor

    Kian Peng Koh

    Article

    Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi

    Insert

    tetracyclin-transactivator (rtTA)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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