We narrowed to 11,588 results for: EMB
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Plasmid#162936PurposeGateway attB4/attB1r entry clone for baculovirus expression of 3xFLAG tagged proteins using the polyhedrin promoterDepositorInsertTriple FLAG epitope tag fusion, AcMNPV polyhedrin promoter
UseSynthetic BiologyAvailable SinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E11: attB5r-RU5u-His6-nostop-attB1r
Plasmid#162953PurposeGateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteinsDepositorInsertHis6 purification tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyAvailable SinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
C417-E06: attB4-polyhedrinp>MBP-nostop-attB1r
Plasmid#162935PurposeGateway attB4/attB1r entry clone for baculovirus expression of MBP tagged proteins using the polyhedrin promoterDepositorInsertMaltose-binding protein fusion, AcMNPV polyhedrin promoter
UseSynthetic BiologyAvailable SinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E09: attB5r-RU5u-FLAG-nostop-attB1r
Plasmid#162951PurposeGateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteinsDepositorInsertFLAG epitope tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyAvailable SinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
C513-E06: attB5r-pA-polyhedrinp>-attB1r
Plasmid#162948PurposeGateway attB5r/attB1r entry clone containing an AcMNPV polyhedrin promoter with upstream polyA, for use in construction of polycistronic baculovirus expression vectorsDepositorInsertAcMNPV polyhedrin promoter with upstream polyA sequence
UseSynthetic BiologyAvailable SinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E59: attB5r-RU5u-2xHA-nostop-attB1r
Plasmid#162966PurposeGateway attB5r/attB1r entry clone for generation of N-terminal dual HA-tagged fusion proteinsDepositorInsertDual HA epitope tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E42: attB5r-RU5u-Strep2-nostop-attB1r
Plasmid#162961PurposeGateway attB5r/attB1r entry clone for generation of N-terminal Strep2-tagged fusion proteinsDepositorInsertStrep2 purification tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E26: attB5r-RU5u-myc-nostop-attB1r
Plasmid#162960PurposeGateway attB5r/attB1r entry clone for generation of N-terminal Myc-tagged fusion proteinsDepositorInsertMyc epitope tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTEF1-UDH
Plasmid#166679PurposeYeast integrative plasmid for expressing P. syringae uronate dehydrogenase, with TEF1 promoter. Contains leucine auxotrophic marker (K. lactis LEU2).DepositorInsertUDH
UseSynthetic BiologyExpressionYeastPromoterTEF1Available SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 AtU6-26:AsCas12a_DR (GB1442)
Plasmid#160574PurposeAsCas12a direct repeat sequence fused to the A. thaliana U6-26 promoter used for the assembly of gRNA expression cassettes.DepositorInsertAtU6-26:AsCas12a_DR
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 nickase D10A Cas9 (GB1691)
Plasmid#160588PurposeNickase D10A Cas9 for Nt fusion.DepositorInsertnickase D10A Cas9
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnma1a
Plasmid#164951PurposeZebrafish kcnma1a gene CDSDepositorInsertkcnma1a (kcnma1a Zebrafish)
UsePcr cloning vectorAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnmb2a
Plasmid#164953PurposeZebrafish kcnmb2a gene CDSDepositorInsertkcnmb2a (kcnmb2 Zebrafish)
UsePcr cloning vectrorAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnmb2b
Plasmid#164954PurposeZebrafish kcnmb2b gene CDSDepositorInsertkcnmb2b (si:ch211-247n2.1 Zebrafish)
UsePcr cloning vectorAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnmb3
Plasmid#164955PurposeZebrafish kcnmb3 gene CDSDepositorInsertkcnmb3 (si:ch211-38m6.7 Zebrafish)
UsePcr cloning vectorAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnt1b
Plasmid#164962PurposeZebrafish kcnt1b gene CDSDepositorInsertkcnt1b (si:dkey-21e5.1 Zebrafish)
UsePcr cloning vectorAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTricHis-ILY-HB2lock-no charge
Plasmid#160532PurposeExpression of inserted ILY variantDepositorInsertILY-HB2lock-no charge
TagsHis-TagExpressionBacterialMutationY208C, D322C, E337A, N338A, K341A, N342AAvailable SinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTricHis-ILY-HB1lock-no charge
Plasmid#160525PurposeExpression of inserted ILY variantDepositorInsertILY-HB1lock-no charge
TagsHis-TagExpressionBacterialMutationA221C, N404C, E337A, N338A, K341A, N342AAvailable SinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Prham-M.PstI-p15A-aadA
Plasmid#149340Purposeexpresses M.PstI DNA methyltransferase in E. coliDepositorInsertM.,PstI
ExpressionBacterialPromoterrhamnoseAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
D78_Ld_362280
Plasmid#135891PurposeExpression of Leishmania donovani ectodomain in mammalian cellsDepositorInserttransmembrane emp24 domain-containing protein
Tagsbiotinylation sequence and His tagExpressionMammalianAvailable SinceJuly 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R367
Plasmid#138237PurposeExpresses the split mCherry reporter interrupted by the LP-Hel intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R368
Plasmid#138238PurposeExpresses the split mCherry reporter interrupted by the MP-B-DnaB artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R369
Plasmid#138239PurposeExpresses the split mCherry reporter interrupted by the MP-K-gp53 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R370
Plasmid#138240PurposeExpresses the split mCherry reporter interrupted by the MP-C-gp206 artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R372
Plasmid#138242PurposeExpresses the split mCherry reporter interrupted by the PP-Phi artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R359
Plasmid#138229PurposeExpresses the split mCherry reporter interrupted by the CP-Thy1 artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
D62_Ld_362720
Plasmid#135875PurposeExpression of Leishmania donovani ectodomain in mammalian cellsDepositorInsertmembrane-bound acid phosphatase 2, putative
Tagsbiotinylation sequence and His tagExpressionMammalianAvailable SinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
D46_Ld_362700
Plasmid#135860PurposeExpression of Leishmania donovani ectodomain in mammalian cellsDepositorInsertmembrane-bound acid phosphatase precursor
Tagsbiotinylation sequence and His tagExpressionMammalianAvailable SinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
D74_Ld_291730
Plasmid#135887PurposeExpression of Leishmania donovani ectodomain in mammalian cellsDepositorInsertendosomal integral membrane protein, putative
Tagsbiotinylation sequence and His tagExpressionMammalianAvailable SinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
D83_Ld_060380
Plasmid#135896PurposeExpression of Leishmania donovani ectodomain in mammalian cellsDepositorInsertPresent in the outer mitochondrial membrane proteome 35
Tagsbiotinylation sequence and His tagExpressionMammalianAvailable SinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFLinkC-AI(G3)-PDZ(GPG-G)FN3(GGS4)-TVMV
Plasmid#137117PurposeProtease Switch Clone ID: 2-A5DepositorInsertAI(G3)-PDZ(GPG-G)FN3(GGS4)-TVMV
UseSynthetic BiologyAvailable SinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pJET1.2_Rab28_Sense
Plasmid#124441PurposePlasmid for sense in situ probe in vitro transcriptionDepositorAvailable SinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pESC/CURT_fluoD
Plasmid#118001PurposeExpression of CURT_flouD controlled by the Gal1 promoterDepositorInsertCURT_flouD
UseYeast/bacteria binary vectorExpressionYeastPromoterGal1Available SinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACEBAC1-CoREST-C
Plasmid#109159PurposeEncodes a human C-terminal CoREST fragment with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman C-terminal CoREST, sequence-optimized for insect cells (RCOR1 Human)
Tags3xFLAGExpressionInsectPromoterPolyhedrinAvailable SinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSG73-β1ARts-sfGFP
Plasmid#102459PurposeExpresses a fusion protein of thermostabilized β1 adrenergic receptor and sfGFP with a C-terminal His tag. The expression is controlled by a OR2-OR1-Pr promoter.DepositorArticleInsertβ1AR
TagsHis tag and sfGFPExpressionBacterialPromoterOR2-OR1-PrAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 p37delta2-81
Plasmid#113502PurposeBacterial expression of GST-tagged p37 lacking the N-terminal AA 2-81DepositorInsertp37 (Ubxn2b Mouse)
TagsGST-PreScission protease siteExpressionBacterialMutationdelta 2-81Available SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-MMP
Plasmid#112632PurposeTwo SpyCatchers linked by an elastin-like polypeptide with an MMP-cleavable sequence and a C-terminal SnoopCatcherDepositorInsertTriCatcher-MMP
TagsHis6 and TEV cleavage siteExpressionBacterialMutationcentral matrix metalloproteinase-cleavable sequen…PromoterT5Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
p005-RFP-weak
Plasmid#108316PurposeSynthetic RFP based on Corynactis spp under weak synthetic constitutive promoter pFAB4024; kanamycin resistant.DepositorInsertRFP under controlf of weak synthetic constitutive promoter pFAB4024
ExpressionBacterialPromoterpFAB4024Available SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
gnu cDNA LD12084
Plasmid#112973PurposeCloning vector carrying the gnu cDNADepositorInsertgnu (gnu Fly)
ExpressionBacterialAvailable SinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt13C(WT)
Plasmid#86576PurposeExpresses cleavable human full-length Akt1(WT)DepositorInsertAkt1 (AKT1 Human)
Tags10xHisExpressionInsectMutation3C cleavage site inserted at pos. 130Available SinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJK139
Plasmid#73315PurposePas2p peroxisomal membrane biogenesis proteinDepositorInsertPas2p peroxisomal membrane targeting protein
TagsHis6 and c-mycExpressionYeastMutationEncodes a glycine-204 to serine mutationPromoterAOX1Available SinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTAct2 (GB0210)
Plasmid#68191PurposeProvides the Arabidopsis thaliana Actin2 gene terminator as a level 0 GoldenBraid partDepositorInsertAtAct2 terminator
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
p5'UTRAct2 (GB0193)
Plasmid#68178PurposeProvides the 5'-untranslated region of the Arabidopsis thaliana actin2 gene as a level 0 GoldenBraid partDepositorInsert5'UTR AtAct2
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
FCIV1-GR SS287AA
Plasmid#72703PurposeLentiviral expression of GR Phospho-deficient mutant (2nd generation)DepositorInsertGR (Nr3c1 Rat)
UseLentiviralTagsIRES-VenusExpressionMammalianMutationSS287AAPromoterubiquitinAvailable SinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGB 1alfa1R Tnos:Hygromycin:PNos (GB0235)
Plasmid#68213PurposeTranscriptional unit for Hygromycin resistance gene plant expressionDepositorInsertHyg
UseSynthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnosAvailable SinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only