We narrowed to 50,495 results for: ang
-
Plasmid#100018PurposeBacterial expression of fluorescent protein casette - cpsfGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkersDepositorInsertcpsfGFP-LSSmOrange
UseTags6x HIS tagExpressionBacterialMutationLeu and Glu N-term linker and Leu and Pro C-term ā¦PromoterT7Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
p221z-erTagRFP
Plasmid#71266PurposeEntry clone containing ER-localized TagRFP. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertTagRFP
UseGatewayTagsER retention signal HDEL and signal peptide (ER)ā¦ExpressionMutationPromoterAvailable SinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJH4169
Plasmid#200108PurposePrig-3 FRT let858 (stop) FRT snb-1::GFP unc-54 3' UTR C.elegans AVA and other neurons expression of snb GFPDepositorAvailable SinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFastBacDual with LFn + Needle
Plasmid#84868PurposeBaculovirus Expression of LFn-Needle fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel proteinDepositorInsertsLFn
Needle
UseBaculovirusTags6xHisExpressionInsectMutationPromoterPolyhedrinAvailable SinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEF005-sgRNA-shuffle-in
Plasmid#104441PurposeProvide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF004.DepositorInsertsgRNA-transRNA transcription by U6 promoter
UseCRISPRTagsExpressionBacterial and PlantMutationPromoterAvailable SinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF004-sgRNA-shuffle-in
Plasmid#104440PurposeProvide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF005DepositorInsertsgRNA-transRNA transcription by U6 promoter
UseCRISPRTagsExpressionBacterial and PlantMutationPromoterU6 promoterAvailable SinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
ATG9A-2STrep
Plasmid#210359PurposeExpresses ATG9A fused with 2Strep in mammalian cellsDepositorAvailable SinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
PARD3_PDZ_3
Plasmid#103959PurposeProtein expression and purification of PAR3 PDZ3 domainDepositorAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFIV-H1-Puro-hPolĆ
Plasmid#18663DepositorInsertshRNA specific to human DNA polymerase beta (POLB Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationnonePromoterAvailable SinceOct. 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-dCas9-MQ1(S317A)-EGFP
Plasmid#89636PurposeTargeted CpG methylationDepositorInsertsite-specific DNA-methyltransferase SssI
UseCRISPRTags3*FLAG and 6*HisExpressionMammalianMutationS317A, AGC-->GCCPromoterCMVAvailable SinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-CFL-SN-P2A-GCaMP6f
Plasmid#181742PurposeAAV vector for expressing CFL-SN and GCaMP6fDepositorInsertCFL-SN-P2A-GCaMP6f
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable SinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
p221k-erTq2CFP
Plasmid#71264PurposeEntry clone containing ER-localized Turqoise2. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway system.DepositorInsertTq2CFP
UseGatewayTagsER retention signal HDEL and signal peptide (ER)ā¦ExpressionMutationPromoterAvailable SinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSET a MatryoshCaMP6s
Plasmid#100019PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.DepositorInsertMatryoshCaMP6s
UseTags6x HIS tag and Xpress_EK tagExpressionBacterialMutationPromoterT7Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBacDual with LFn + Fla
Plasmid#84866PurposeBaculovirus Expression of LFn -Fla fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel proteinDepositorInsertsLFn
Fla
UseBaculovirusTagsExpressionInsectMutationPromoterPolyhedrinAvailable SinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) M-Ras(WT)
Plasmid#224287PurposeCell transfection and expression of M-RasDepositorAvailable SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWPI-IRES-eGFP-Mefv iso1
Plasmid#92104PurposeExpression in eukaryotic cells and generating stable cell linesDepositorAvailable SinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-IFITM3-F8-TAG
Plasmid#122475Purposeexpresses human IFITM3 with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.DepositorInsertIFITM3 (IFITM3 Human)
UseTagsHAExpressionMammalianMutationChanged Phenylalanine 8 to amber codonPromoterAvailable SinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGC(2.1)-U6.3
Plasmid#170516PurposePCR template vector for amplifying gRNAcore(2.1)-pU6.3 promoter fragment; used together with pAC-CR7T-gRNA2.1-nlsBFPDepositorInsertgRNAcore(2.1)-pU6.3
UsePcr template vector for amplifying grnacore(2.1)-ā¦TagsExpressionMutationPromoterAvailable SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
PTPN13_PDZ_4
Plasmid#103922PurposeProtein expression and purification of PTN13 PDZ4 domainDepositorAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnn3
Plasmid#164959PurposeZebrafish kcnn3 gene CDSDepositorAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
Venus-7aa-ActA-pEGFP-C1
Plasmid#177405PurposeTo express Venus fused to mitochondria outer membrane targeting sequence "ActA". Venus faces the cytosol.DepositorInsertActA tail anchor (actA Listeria monocytogenes)
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
OA-1067A1
Plasmid#164847PurposeExpresses four gRNA targeting BTub gene with 3xp3-eGFP markerDepositorInsert4 gRNAs and 3xP3-eGFP
UseTagsExpressionInsectMutationPromoterAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-P1-LoxP-Neo-N-miRFP670-C
Plasmid#159434PurposeCRISPR knock-in donor construct with fluorescent reporter and removable selectable markerDepositorInsertsNeomycin
miRFP670
UseCRISPRTags3' linker pair 1, 5' linker pair 1, C tā¦ExpressionMutationPromoterSV40Available SinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15b-eGO-Matryoshka-LE-LP
Plasmid#100017PurposeBacterial expression of fluorescent protein casette - cpEGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkersDepositorInsertcpEGFP-LSSmOrange
UseTags6x HIS tagExpressionBacterialMutationLeu and Glu N-term linker and Leu and Pro C-term ā¦PromoterT7Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag FKHR H215R AAA mutant
Plasmid#13510DepositorInsertFKHR H215R AAA (FOXO1 Human)
UseTagsFlagExpressionMammalianMutationH215R T24A S256A S319APromoterAvailable SinceDec. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
Direct-seq_lentiGuide-Puro-Loop2-8A8G
Plasmid#157983PurposeExpresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.DepositorInsertS. pyogenes sgRNA cassette (Loop2-8A8G)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available SinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
p15a_Kan_MLS_Rosa26_Short_CRISPRDel_FNeoStop_PStop_WPRE_bgh_pA
Plasmid#97012PurposeRosa26 targeting vector for intersectional Cre and FLP responsive cassetteDepositorTypeEmpty backboneUseCRISPR and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable SinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pR008_APAD
Plasmid#204818PurposeExpression Apobec1-ADAR2dd fusion protein as PIE-RBP controlDepositorInsertrat Apobec1 and human ADAR2 deaminase domain with engineered sites (Apobec1 Rat)
UseTagsHA and Flag and huADAR2ddExpressionMammalianMutationPromoterchicken β-actin promoterAvailable SinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2-elavl3-WHaloCaMP1a
Plasmid#205312PurposeExpression of WHaloCaMP1a in zebrafish from the elavl3 promoter (pan-neuronal expression)DepositorInsertWHaloCaMP1a
UseZebrafish expressionTagsExpressionMutationPromoterAvailable SinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dLight1.3a
Plasmid#111055PurposeExpresses dLight1.3a in mammalian cellsDepositorInsertdLight1.3a
UseTagsFlag tagExpressionMammalianMutationPromoterCAGAvailable SinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV CB6 FFLuc-miR122
Plasmid#35656DepositorAvailable SinceApril 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAS18
Plasmid#158787PurposeRibosome-tagged GCaMP (soma localized) expression pan-neuronally C. elegansDepositorInsertPtag-168::GCaMP6m::rpl-1a
UseTagsExpressionWormMutationPromoterAvailable SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
OA-1067K
Plasmid#164848PurposeExpresses four gRNA targeting Mhc gene with 3xp3-tdTomato markerDepositorInsert4 gRNAs and 3xP3-tdTomato
UseTagsExpressionInsectMutationPromoterAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
OA-874PA
Plasmid#164846PurposeExpresses AAEL005635-Cas9 with OpIE2-CFP markerDepositorInsertP10-3'UTR OpIE2-CFP
UseTagsExpressionInsectMutationPromoterAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-TDAG51
Plasmid#32699DepositorAvailable SinceFeb. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40
Plasmid#133560PurposeBackbone vector for CRISPR-HDR insertion of SPARC-Variant constructs (or any module) near the attP40 locusDepositorTypeEmpty backboneUseCRISPRTagsExpressionInsectMutationPromoterAvailable SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGIPz-PD-L1 3SA
Plasmid#121490PurposeExpression of human PD-L1 3SA with PD-L1 shRNADepositorInsertsPD-L1 3SA
humun PD-L1 shRNA
UseLentiviralTagsFlagExpressionMammalianMutationS176A, T180A, S184APromoterCMVAvailable SinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
(282) pcDNA3.1-HA-nGFP-(EAAAK)4-OGT(4,K852A)
Plasmid#160741PurposeExpresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to a GFP nanobodyDepositorInsertHA-nGFP-(EAAAK)4-OGT(4,K852A)
UseTagsHA-TagExpressionMammalianMutationPromoterT7/CMVAvailable SinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
LITE1.0_pAAV_hSyn_CRY2PHR-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA
Plasmid#47454PurposeepiLITE / LITE1.0 CRY2PHR fused to SID4X transcriptional repressor effector domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression.DepositorInsertCRY2PHR-NLS-SID4X
UseAAVTags2A_phiLOV2.1ExpressionMammalianMutationPromoterhSyn human synapsin (mammalian neuronal expressioā¦Available SinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
p164-pAAV-mDlx-FLEX-mScarlet
Plasmid#204689PurposeFluorescent reporter under the control of the Dlx promotorDepositorInsertmScarlet
UseAAV and Cre/LoxTagsmScarlet palmitoylationExpressionMammalianMutationPromotermDlxAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
PL752-CLSTN2-LA-p2a-tdTomato-hGHpA-PGK-puroR-CLSTN2-RA
Plasmid#191685PurposeFor knocking p2a-tdTomato into CLSTN2 locusDepositorInsertCLSTN2 homology arms and p2a-tdTomato
UseTagsExpressionMammalianMutationWTPromoterAvailable SinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
tdp43-EGFP construct11
Plasmid#28204DepositorInsertTar DNA binding protein (TARDBP Human)
UseTagsEGFPExpressionMammalianMutationaa193-274 onlyPromoterAvailable SinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFastBacDual with LFn + FlaAAA
Plasmid#84867PurposeBaculovirus Expression of LFn-FlaAAA fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.DepositorInsertsLFn
FlaAAA
UseBaculovirusTags6xHisExpressionInsectMutation3 C-terminal leucines have been replaced by alaniā¦PromoterPolyhedrinAvailable SinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAD-R-iLACCO1.1
Plasmid#208020PurposeBiosensor for intracellular L-lactateDepositorInsertR-iLACCO1.1
UseTagsExpressionBacterialMutationPromoterAvailable SinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10714
Plasmid#183959PurposeU6-crKlf4-CAG-hyperdCas12a-miniVPRDepositorInsertsHyperdCas12a
crRNA targeting Klf4
UseCRISPRTagsHAExpressionMammalianMutationD832A, D156R, E292R, D235R, D350RPromoterCAG and U6Available SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHDR-eGFP (pXK-1647)
Plasmid#208824PurposeThe HDR-based surrogate reporter for validating HDR-editing activity of different gene editing tools as well as for helping to screen HDR-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(HDRDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable SinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUAST-SIK3 T196E
Plasmid#52975Purposeinsect expression of SIK3 T196E constitutively active mutantDepositorInsertSIK3 (Sik3 Fly)
UseTagsHAExpressionInsectMutationT196E constitutively active mutantPromoterHsp70Available SinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
PL752-OTX2-LA-p2a-EGFPnls-hGHpA-PGK-puroR-OTX2-RA
Plasmid#191690PurposeFor knocking p2a-EGFP into OTX2 locusDepositorInsertOTX2 homology arms and p2a-EGFP
UseTagsExpressionMammalianMutationWTPromoterAvailable SinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
PL752-LMX1A-LA-p2a-tdTomato-hGHpA-PGK-puroR-LMX1A-RA
Plasmid#191683PurposeFor knocking p2a-tdTomato into LMX1A locusDepositorInsertLMX1A homology arms and p2a-tdTomato
UseTagsExpressionMammalianMutationWTPromoterAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH-PD-L1 3SA/4NQ
Plasmid#121491PurposeExpression of human PD-L1 3SA/4NQ with PD-L1 shRNADepositorInsertPD-L1
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable SinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only