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pcDNA3.1(+)-FluoSTEP-AKAR

Plasmid

#181844

Purpose

Genetically encoded FRET-based sensor for monitoring PKA activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

FluoSTEP-AKAR

Tags

mRuby2 and sfGFP(1-10)

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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CD8-GFP-FKBP

Plasmid

#100751

Purpose

Expression of GFP-FKBP at the plasma membrane in mammalian cells. Plasma membrane targeting is via CD8 alpha chain.

Depositor

Stephen Royle

Article

Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1

Insert

CD8A (CD8A Human)

Tags

FKBP and GFP

Expression

Mammalian

Mutation

Truncated

Promoter

CMV

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pFudioFRTPSD-95TealW

Plasmid

#134299

Purpose

Expresses PSD95 fused to Teal fluorescent protein upon coexpression with the recombinase flippase (Flp)

Depositor

Elly Nedivi

Article

Subramanian et al Cell Rep. 2019 Aug 6;28(6):1584-1595.e5.

Insert

PSD-95-Teal (Dlg4 Rat, Mouse)

Use

Lentiviral; Flp/frt

Tags

Teal

Expression

Mammalian

Promoter

UbC

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pARC8-LambdaRedBeta-EcSSB

Plasmid

#162572

Purpose

Arabinose inducible recombineering plasmid encoding Lambda-Red Beta with EcSSB for use with dsDNA templates and enhanced recombination efficiency.

Depositor

George Church

Article

Filsinger et al Nat Chem Biol. 2021 Jan 18. pii: 10.1038

Inserts

Lambda Red-Beta
E. coli SSB

Expression

Bacterial

Promoter

pBAD

Available Since

May 22, 2021

Availability

Academic Institutions and Nonprofits only

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pET30-ARH3-His-Sumo-HA

Plasmid

#111578

Purpose

Expresses ARH3 (amino acids 14-363) in E. coli

Depositor

Thomas Muir

Article

Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

Insert

ARH3 (ADPRS Human)

Tags

6xHis-Sumo and HA

Expression

Bacterial

Mutation

deleted amino acids 1-13

Promoter

T7

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pXPR_051_dCas9-KRAB-HA

Plasmid

#228936

Purpose

All-in-one dCas9-KRAB-MeCP2 plasmid for cloning of custom sgRNA

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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miniCGBE1 (pBM1219)

Plasmid

#140253

Purpose

CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

BE4max(R33A)-ΔUGI

Expression

Mammalian

Mutation

R33A in rAPOBEC1, D10A in Cas9

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-ZDHHC7

Plasmid

#231656

Purpose

Expresses human ZDHHC7 protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

ZDHHC7 (ZDHHC7 Human)

Tags

3×Flag Tag

Expression

Mammalian

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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SpCas9 TadCBEd-V106W

Plasmid

#193839

Purpose

Express TadCBEd-V106W (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadA-CDd V106W-SpCas9 D10A-2xUGI

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDY1462_SERPINA1_CCA32_Caspase

Plasmid

#193194

Purpose

Express Caspase conditional upon RNA transcript

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

Insert

iCaspase9 (CASP9 Human)

Expression

Mammalian

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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ABE8.17-m

Plasmid

#136298

Purpose

expresses ABE8.17-m in mammalian cells

Depositor

Nicole Gaudelli

Article

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Insert

ABE8.17-m

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-anti-GFP(LaG17) PAGER(TF)

Plasmid

#229997

Purpose

Expresses anti-GFP PAGER(TF) (high reversibility) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

IL2SP-Aro6-LaG17-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4

Use

AAV

Expression

Mammalian

Mutation

V360A/R361A on KORD

Promoter

CMV

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pWZ854

Plasmid

#208116

Purpose

mSwAP-In marker cassette 2 with 5’PB-mNeongreen-HPRT1-Neo

Depositor

Jef Boeke

Article

Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

Insert

mNeonGreen-HPRT1-Neo

Tags

T2A

Expression

Bacterial and Mammalian

Promoter

EF1alpha

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAAVS1-NDi-CRISPRd (ABI-dCas9-mCherry)

Plasmid

#216531

Purpose

Targeting vector for CRISPRd generation

Depositor

Makiko Iwafuchi

Article

Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

Insert

ABI-dCas9-mCherry

Use

CRISPR

Tags

mCherry

Expression

Mammalian

Promoter

TRE3G

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-RIα-EGFP

Plasmid

#181840

Purpose

GFP-tagged PKA RIα subunit for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

RIα-EGFP (PRKAR1A Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pX330-RPA194

Plasmid

#247352

Purpose

Expresses SpCas9 and a sgRNA targeting the human RPA194 loci for knock-in.

Depositor

Kazuhiro Maeshima

Article

Ide et al Sci Adv. 2020 Oct 14;6(42). pii: 6/42/ea

Insert

RPA194 (POLR1A Human)

Use

CRISPR

Promoter

U6

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pIVT/CamKII gamma CA

Plasmid

#122142

Purpose

For making constitutively active CamKII gamma RNA

Depositor

Carmen Williams

Article

Miao et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

Insert

constitutively active CamKII gamma (Camk2g Mouse)

Use

For in vitro transcription

Mutation

First 291 amino acid residues of CaMKII gamma

Promoter

T7

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pSL1425 (pSPIN, pCDF backbone)

Plasmid

#160735

Purpose

Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA and has BsaI sites for spacer cloning. pCDF backbone.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST proteins, crRNA, and donor DNA

Expression

Bacterial

Promoter

J23119

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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Cas12f-GE ver4.1

Plasmid

#176544

Purpose

Expresses Cas12f-GE in mammalian cells

Depositor

Yong-Sam Kim

Article

Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

Inserts

Cas12f-GE ver4.1
Cas12f-GE ver4.1

Expression

Mammalian

Promoter

U6 and chicken β-actin

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-anti-PDL1(KN035) PAGER(TF)

Plasmid

#230003

Purpose

Expresses anti-PDL1 PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

IL2SP-Aro6-KN035-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4

Use

AAV

Expression

Mammalian

Mutation

V360A/R361A on KORD

Promoter

CMV

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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