We narrowed to 9,825 results for: 158

1. pDY1623 NOLC1 sgRNA 2

   Plasmid

   #234834

   Purpose

   sgRNA 2 for NOLC1 STITCHR insertion

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

   Insert

   NOLC1 sgRNA (NOLC1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-GGamma2-GFP2

   Plasmid

   #166774

   Purpose

   Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma2-GFP2 (GNG2 Synthetic, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains an N-terminal GFP2 and GSAG linker

   Promoter

   CMV

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-GGamma9-GFP2

   Plasmid

   #140991

   Purpose

   Encodes a G gamma subunit (GNG9) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma9-GFP2 (GNGT2 Human)
   

   Use

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX037

   Plasmid

   #167156

   Purpose

   MoClo-compatible Level2 vector for autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, nnH3H, nnLuz and nnCPH. Does not contain NpgA.

   Depositor

   Karen Sarkisyan

   Article

   Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

   Insert

   caffeic acid cycle genes
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pNOS, p35s

   Available since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pD649-HAsp-KIR2DS1-COMP5AP-AviTag-9xHis

   Plasmid

   #157239

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   KIR2DS1 (KIR2DS1 Human)
   

   Use

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/SP6

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. PEmax_AAVS1_knockin_HDR_donor

   Plasmid

   #223000

   Purpose

   HDR donor plasmid for PEmax knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PEmax-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PEmax
   

   Use

   Hdr donor

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-GGamma12-GFP2

   Plasmid

   #166781

   Purpose

   Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma12-GFP2 (GNG12 Synthetic, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains an N-terminal GFP2 and GSAG linker

   Promoter

   CMV

   Available since

   April 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1-GGamma3-GFP2

   Plasmid

   #166775

   Purpose

   Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma3-GFP2 (GNG3 Synthetic, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains an N-terminal GFP2 and GSAG linker

   Promoter

   CMV

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-GGamma7-GFP2

   Plasmid

   #166778

   Purpose

   Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma7-GFP2 (GNG7 Synthetic, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains an N-terminal GFP2 and GSAG linker

   Promoter

   CMV

   Available since

   April 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28-SpRY-HF1-NLS-6xHis (gbr2102)

    Plasmid

    #181744

    Purpose

    bacterial expression plasmid for SpRY-HF1 with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag

    Depositor

    Benjamin Kleinstiver

    Article

    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

    Insert

    bacterial codon optimized SpRY-HF1
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS(SV40)-6xHis and MKIEE MBP tag

    Expression

    Bacterial

    Mutation

    SpRY-HF1, SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E…

    Promoter

    T7

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-GGamma11-GFP2

    Plasmid

    #166780

    Purpose

    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma11-GFP2 (GNG11 Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains an N-terminal GFP2 and GSAG linker

    Promoter

    CMV

    Available since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-GGamma13-GFP2

    Plasmid

    #140992

    Purpose

    Encodes a G gamma subunit (GNG13) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma13-GFP2 (GNG13 Human)
    

    Use

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. EK0397 3FLAG-ADAR1-p150-HIS (pEAQ)

    Plasmid

    #191172

    Purpose

    Human ADAR1, p150 isoform in plant-expressable format.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    3FLAG-ADAR1-p150-HIS (plant) (ADAR Human)
    

    Use

    Tags

    3FLAG and HIS

    Expression

    Plant

    Mutation

    WT

    Promoter

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLX304-YAP1(S94A)

    Plasmid

    #59145

    Purpose

    YAP1 serine 94 o alanine mutants which disrupts binding to TEAD transcription factors

    Depositor

    William Hahn

    Article

    Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    S94A

    Promoter

    CMV

    Available since

    Aug. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pARC8-LambdaRedBeta-EcSSB-EcMutLE32K

    Plasmid

    #162576

    Purpose

    Arabinose inducible recombineering plasmid encoding Lambda-Red Beta with EcSSB for use with dsDNA templates and enhanced recombination efficiency. Contains a dominant negative mismatch repair protein.

    Depositor

    George Church

    Article

    Filsinger et al Nat Chem Biol. 2021 Jan 18. pii: 10.1038

    Inserts

    Lambda Red-Beta
    E. coli SSB
    EcMutLE32K
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E32K mutated to give dominant negative phenotype

    Promoter

    pBAD

    Available since

    May 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCWXPGR-pTF-dnTCF4

    Plasmid

    #114277

    Purpose

    TET-inducible expression of a dominant negative form of the TCF4 transcription factor

    Depositor

    Patrick Salmon

    Article

    Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

    Insert

    TCF4 (TCF4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    deletion of AA 1 to 31

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-BRD9

    Plasmid

    #65380

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    BRD9 (BRD9 Human)
    

    Use

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pmRFP-N1 human cofilin R21Q

    Plasmid

    #51279

    Purpose

    expresses human cofilin R21Q mutant fused to mRFP in mammalian cells

    Depositor

    James Bamburg

    Article

    Mi et al PLoS One. 2013 Dec 31;8(12):e83609. doi:

    Insert

    cofilin 1 (CFL1 Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    R21Q, non-rod-forming mutation that can be used t…

    Promoter

    CMV

    Available since

    Feb. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pFastbac1-Flag-FANCD2

    Plasmid

    #134904

    Purpose

    codon optimized expression and purification of human FANCD2 in insect cells using baculovirus

    Depositor

    Andrew Deans

    Article

    Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

    Insert

    FANCD2 (FANCD2 Human)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    fully synthetic, codon optimized for insect expre…

    Promoter

    polyhedron

    Available since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. Circular 200,100 (RAB7A)

    Plasmid

    #170118

    Purpose

    AAV vector carrying a guide RNA targeting the human RAB7A mRNA

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA (RAB7A Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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