We narrowed to 26,426 results for: CAL

1. pCDB364

   Plasmid

   #110275

   Purpose

   N-terminal 10-His DsbC TEV-peptide fusion

   Depositor

   Christopher Bahl

   Article

   Buchko et al Protein Sci. 2018 Aug 26. doi: 10.1002/p

   Type

   Empty backbone

   Use

   Tags

   10-His, TEV cleavage peptide, and mature DsbC (i.…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. SLC5A3_pCSdest

   Plasmid

   #53836

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   SLC5A3 (SLC5A3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SP6 and CMV

   Available since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pDOC-K-glmS

   Plasmid

   #158058

   Purpose

   Donor plasmid for chromosomal integrations of any sequence at a locus downstream of the glmS gene in Enterobacteriaceae using kanamycin resistance as a selectable marker

   Depositor

   Mark Webber

   Article

   Holden et al Microbiology (Reading). 2020 Nov 23. doi

   Inserts

   glmS homologous region 1
   glmS homologous region 2 + MCS3
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-eGtACR1-mRuby2-ST

   Plasmid

   #109136

   Purpose

   Anion channelrhodopsin GtACR1 fused to ER export signal, mRuby2 and targeted to the neuronal soma and proximal dendrites

   Depositor

   Hillel Adesnik

   Article

   Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

   Insert

   eGtACR1-mRuby2-ST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV Syn intron Psam4GlyR m3m4 HA

   Plasmid

   #196041

   Purpose

   AAV for Chemogenetic plasmids

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2019 Mar 14. pii: science.aav52

   Insert

   Psam4GlyR m3m4 HA
   

   Use

   AAV

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXs-puro HA-WIPI-1

   Plasmid

   #38273

   Depositor

   Noboru Mizushima

   Article

   Itakura et al Autophagy. 2010 Aug;6(6):764-76.

   Insert

   WD repeat domain phosphoinositide-interacting protein 1 (WIPI1 Human)
   

   Use

   Retroviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pMET7-GAG-GW

   Plasmid

   #194634

   Purpose

   Gateway destination vector for mammalian expression of Virotrap bait constructs

   Depositor

   Sven Eyckerman

   Article

   Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

   Insert

   p55 GAG
   

   Use

   Tags

   PQS1

   Expression

   Mammalian

   Mutation

   Promoter

   SRalpha

   Available since

   Jan. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC57-PB-PGK-tetR-KRAB-2A-Puro

   Plasmid

   #129536

   Purpose

   PiggyBac transposon carrying tetR-KRAB-2A-Puro under the PGK promoter.

   Depositor

   Hideyuki Okano

   Article

   Tsujimura et al Elife. 2020 May 5;9. pii: 47980. doi: 10

   Insert

   PB-PGK-tetR-KRAB-2A-Puro
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   hPGK promoter

   Available since

   Dec. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTS94 pHAGE2 CMVtetO2 GFP-22xGS-3C-MCS

   Plasmid

   #199347

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-3C-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP-3C

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMSCV-FKBP-empty

    Plasmid

    #221239

    Purpose

    Expression of FLAG-4xFKBP scaffold in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    FLAG-4xFKBP scaffold
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330-sgRNA_Dgcr8_1

    Plasmid

    #73525

    Purpose

    Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

    Depositor

    Constance Ciaudo

    Article

    Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

    Insert

    DGCR8
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

12. pFA6a-TRP1-PGAL1-3HA

    Plasmid

    #41609

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    3xHA, TRP1 reverse orientation, and yeast ADH1 te…

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. LZF39: Cre-off hSyn-CheRiff-CFP (FAS)

    Plasmid

    #104118

    Purpose

    synOptopatch construct

    Depositor

    Adam Cohen

    Article

    Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

    Insert

    CheRiff-CFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin I

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. CAG:: ChR2HA-2a-hM4D

    Plasmid

    #52520

    Purpose

    Co-expresses HA tagged channelrhodopsin and hM4D (Gi)

    Depositor

    Scott Sternson

    Article

    Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

    Insert

    ChR2-2a-hM4D
    

    Use

    Tags

    2xHA on ChR2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pICH47732:PtFCP:ShBle

    Plasmid

    #104893

    Purpose

    Expresses ShBle with FCP promoter/terminator, Golden Gate Assembly, for Phaeodactylum transformation.

    Depositor

    Thomas Mock

    Article

    Graff van Creveld et al Front Microbiol. 2021 Sep 8;12:688199. d

    Insert

    ShBle
    

    Use

    Phaeodactylum; golden gate assembly

    Tags

    PtFCP promoter and terminator

    Expression

    Mutation

    Promoter

    Available since

    Dec. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1 FLIPE-100u

    Plasmid

    #13543

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E S118W
    

    Use

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    S118W in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. MAC_C_SFXN1

    Plasmid

    #111676

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    SFXN1 (SFXN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMET7-GAG-HRAS

    Plasmid

    #80604

    Purpose

    Mammalian expression vector that expresses the HIV-1 GAG protein with HRAS fused to it

    Depositor

    Sven Eyckerman

    Article

    Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

    Insert

    HIV-1 p55 GAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SRalpha

    Available since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV.Syn-FLEX.iGABASnFR.F102G.mRuby3

    Plasmid

    #112170

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Insert

    iGABASnFR.F102G
    

    Use

    AAV

    Tags

    mRuby3

    Expression

    Mutation

    F102G

    Promoter

    Synapsin

    Available since

    July 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX330-GFP-SFPQ

    Plasmid

    #97084

    Purpose

    Encodes an sgRNA that creates a DSB at the promoter of human SFPQ gene.

    Depositor

    Archa Fox

    Article

    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

    Insert

    sgRNA GFP-SFPQ
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. miR-vector-RFP pCDNA

    Plasmid

    #97143

    Purpose

    for expression of miRNA and TagRFP

    Depositor

    Qing Deng

    Article

    Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

    Type

    Empty backbone

    Use

    Tags

    TagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. human p53-(310-360)

    Plasmid

    #24872

    Depositor

    Cheryl Arrowsmith

    Article

    Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains only the dimeric oligomerization domain …

    Promoter

    Available since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

23. SHC003BSD

    Plasmid

    #133300

    Purpose

    The puromycin selection marker was replaced by blasticidin in SHC003. This construct could be used together with other plasmid contains puromycin selection marker.

    Depositor

    Jingshi Shen

    Article

    Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHH0103_PCNA_PCNA

    Plasmid

    #110022

    Purpose

    Protein expression and purification of PCNA_PCNA

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    PCNA_PCNA (PCNA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pc-GAKIN-Ha

    Plasmid

    #188246

    Purpose

    Express GAKIN in mammalian cells with Ha tag

    Depositor

    Joel Pomerantz

    Article

    Lamason et al Mol Cell. 2010 Dec 10;40(5):798-809. doi

    Insert

    GAKIN (KIF13B Human)
    

    Use

    Tags

    Ha

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBTK1003 (pSL2)

    Plasmid

    #190998

    Purpose

    Expresses red chromoprotein on a broad host range RSF1010 origin plasmid

    Depositor

    Jeffrey Barrick

    Article

    Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

    Insert

    red chromoprotein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    CP25

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBabe-TetCMV-puro-mVenus-LOV

    Plasmid

    #22033

    Purpose

    Mammalian expression of mVenus tagged LOV2 domain

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    mVenus-LOV
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Insert length based on Venus (720bp) + LOV (450bp)

    Promoter

    Available since

    Sept. 11, 2009

    Availability

    Academic Institutions and Nonprofits only

28. LZF42: Cre-off hSyn-QuasAr3-Citrine (FAS)

    Plasmid

    #104116

    Purpose

    synOptopatch construct

    Depositor

    Adam Cohen

    Article

    Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

    Insert

    QuasAr3-Citrine
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin I

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET15b/BOA

    Plasmid

    #82041

    Purpose

    For E.coli expression of 6xHis-Tagged BOA (Burkholderia oklahomensis agglutinin)

    Depositor

    Angela Gronenborn

    Article

    Whitley et al FEBS J. 2013 May;280(9):2056-67. doi: 10

    Insert

    Burkholderia oklahomensis agglutinin
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRc/CMV HA TrkB-T1

    Plasmid

    #39980

    Depositor

    Yves-Alain Barde

    Article

    Bibel et al EMBO J. 1999 Feb 1;18(3):616-22.

    Insert

    TrkB.T1 (Ntrk2 Rat)
    

    Use

    Tags

    BM40 signal sequence and HA

    Expression

    Mammalian

    Mutation

    TrkB.T1 variant; endogenous signal sequence was r…

    Promoter

    CMV

    Available since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

31. pTrcHisA-rat-PTP1B

    Plasmid

    #35987

    Depositor

    Anna Huttenlocher

    Article

    Cortesio et al J Cell Biol. 2008 Mar 10;180(5):957-71.

    Insert

    protein tyrosine phosphatase 1B (Ptpn1 Rat)
    

    Use

    Tags

    His and Xpress Epitope Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBWB424

    Plasmid

    #140638

    Purpose

    RSF1010-Cas9-vanAB gRNA. Vector with constitutive Cas9 and gRNA targeting vanAB locus.

    Depositor

    Keith Tyo

    Article

    Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBluescript SK +/- mTRF1

    Plasmid

    #12303

    Depositor

    Titia de Lange

    Article

    Broccoli et al Hum Mol Genet. 1997 Jan . 6(1):69-76.

    Insert

    mTRF1 (Terf1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

34. pLenti-TK-Firefly-cjBCCIP

    Plasmid

    #122278

    Purpose

    Lentiviral vector for stable expression of Firefly luciferase.

    Depositor

    Demián Cazalla

    Article

    Gorbea et al Elife. 2019 Sep 20;8. pii: 50530. doi: 1

    Inserts

    Firefly luciferase
    cjBCCIP 3'UTR
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLentiCRISPRv2-sgLmnb2-HepmCherry

    Plasmid

    #192830

    Purpose

    Expresses sgRNA targeting Lmnb2 and mCherry from hepatocyte-specific promoter

    Depositor

    Kristin Knouse

    Article

    Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

    Insert

    mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for sgRNA and hepatocyte-specific promoter (HS…

    Available since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAV.hSynap.iGABASnFR.R205A

    Plasmid

    #112162

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Insert

    iGABASnFR.R205A
    

    Use

    AAV

    Tags

    Expression

    Mutation

    R205A (no GABA binding)

    Promoter

    hSynapsin

    Available since

    July 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. px330 RAD21 gRNA

    Plasmid

    #165592

    Purpose

    Insertion of RAD21 degron

    Depositor

    Cornelis Murre

    Article

    Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

    Insert

    RAD21 (Rad21 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. px459 sgFIP200

    Plasmid

    #175024

    Purpose

    CRISPR-Cas9 plasmid targeting exon 3 of human FIP200.

    Depositor

    David Tumbarello

    Article

    Assar et al Front Cell Dev Biol. 2020 Aug 4;8:733. d

    Insert

    RB1CC1 (RB1CC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Lv EF1a HP1cs-Frb2x PGK Puro

    Plasmid

    #102809

    Purpose

    Lentiviral plasmid from FIRE-Cas9 system to express HP1cs-Frb2x fusion protein

    Depositor

    Jerry Crabtree

    Article

    Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

    Insert

    HP1cs-Frb2x
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. mTagRFP-T-Vinculin-23

    Plasmid

    #58030

    Purpose

    Localization: Focal Adhesions, Excitation: 555, Emission: 584

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    Vinculin (VCL Human)
    

    Use

    Tags

    mTagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 4, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits