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We narrowed to 9,400 results for: Coli

Showing: 6481 - 6510 of 9400 results

  2. pPP089

    Plasmid
    #158796
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-3 in MCS1 for expression in E. coli and protein purification
    Depositor
    Jennifer Doudna
    Article
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Insert
    CasPhi-3
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158796-plasmid-map-sequence-id-309513

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  4. pDULE-ABK

    Plasmid
    #49086
    Purpose
    A pyrrolysyl tRNA/tRNA sythetase pair evolved to efficiently incorporate an aliphatic diazirine amino acid into proteins in E. coli and mammalian cells for crosslinking experiments
    Depositor
    Peter Schultz
    Article
    Ai et al Chembiochem. 2011 Aug 16;12(12):1854-7.
    Inserts
    tRNA synthetase
    tRNA for a photocrosslinking amino acid
    Use
    Tags
    Expression
    Bacterial
    Mutation
    L274M, C313A, Y349F
    Promoter
    GlnRS and lpp
    Available Since
    Nov. 6, 2013
    Availability
    Academic Institutions and Nonprofits only
    49086-plasmid-map-sequence-id-396431

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  5. pAC-85b

    Plasmid
    #53282
    Purpose
    Contains crtE, crtI, crtY genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and will produce beta-carotene in E. coli when complemented with a gene encoding phytoene synthase
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E
    Insert
    crtE, crtY, crtI
    Use
    Low copy number bacterial cloning vector
    Tags
    Expression
    Mutation
    Promoter
    endogenous promoters
    Available Since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53282-plasmid-map-sequence-id-82995

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  7. pRL1342

    Plasmid
    #70691
    Purpose
    RSF1010-based vector with extensive polylinker, and resistance to Cm and Em. Shuttle vector suitable for transfer to, and replication in, E. coli and Anabaena (Nostoc) sp. strain PCC 7120.
    Depositor
    Peter Wolk
    Article
    Wolk et al Arch Microbiol. 2007 Dec;188(6):551-63.
    Type
    Empty backbone
    Use
    Bacterial shuttle vector
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Nov. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    70691-plasmid-map-sequence-id-126468

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  8. pDGB1alpha11_SulR

    Plasmid
    #186424
    Purpose
    transcriptional unit for sulfadiazine resistance; plant expression driven by the Pnos promoter; suitable for quintuple assembly
    Depositor
    Lukas Fischer
    Article
    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007
    Insert
    SulR
    Use
    Synthetic Biology; Binary vector for escherichia …
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos
    Available Since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    186424-plasmid-map-sequence-id-369067

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  9. Burden Monitor lambda version

    Plasmid
    #66073
    Purpose
    The burden monitor was cloned inside plasmid pAH63 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cells
    Depositor
    Tom Ellis
    Article
    Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:
    Insert
    GFP
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 21, 2015
    Availability
    Academic Institutions and Nonprofits only

  10. pAC-94N

    Plasmid
    #53281
    Purpose
    Contains crtI, crtY, and crtI genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and produces beta-carotene in E. coli when complemented with a gene encoding geranylgeranyl diphosphate synthase
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E
    Insert
    crtY, crtI, crtB
    Use
    Low copy number bacterial cloning vector
    Tags
    Expression
    Mutation
    Promoter
    endogenous promoters
    Available Since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53281-plasmid-map-sequence-id-82992

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  13. pMP416

    Plasmid
    #134996
    Purpose
    dCas9-m6A Writer. Methylates adenines at local GATC sites. pCAG-dCas9-NLS-3xFLAG-3AC3L linker-EcoDam(N132A)
    Depositor
    Ahmad Khalil
    Article
    Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.
    Insert
    pCAG-dCas9-NLS-3xFLAG-3AC3L linker-EcoDam(N132A)
    Use
    Synthetic Biology
    Tags
    3xFLAG, E coli Dam(N132A), and dCas9
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    134996-plasmid-map-sequence-id-267931

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  15. pG108-K - AhpC-Glow

    Plasmid
    #185135
    Purpose
    Bacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markers BS2 is cloned under the Bacteroides thetaiotaomicron ahpC promoter
    Depositor
    Janina Lewis
    Article
    Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1
    Inserts
    Glow gene
    Bacteroides thetaiotaomicron ahpC promoter
    Use
    Pg106
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Dec. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    185135-plasmid-map-sequence-id-378889

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  17. pLz37_CB_operon_CsoS2_N1-N4

    Plasmid
    #140856
    Purpose
    Halothiobacillus neapolitanus carboxysome operon with full length CsoS2. For heterologous expression of carboxysomes in E. coli.
    Depositor
    David Savage
    Article
    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-
    Insert
    CbbL, CbbS, CsoS2 (full-length), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    140856-plasmid-map-sequence-id-300524

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  19. ins:CFP-NTR

    Plasmid
    #66593
    Purpose
    Specific and conditional cell ablation of beta cells in the zebrafish pancreas
    Depositor
    Didier Stainier
    Article
    Curado et al Dev Dyn. 2007 Apr . 236(4):1025-35.
    Insert
    Insulin promotor driving CFP-nitroreductase
    Use
    Zebrafish expression
    Tags
    CFP fused with N-terminus of nitroreductase
    Expression
    Mutation
    Promoter
    Zebrafish Insulin promoter (approximately 1.2kb)
    Available Since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only

  21. pAC-ZEAX

    Plasmid
    #53274
    Purpose
    Contains crtE, crtB, crtI, crtY and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces zeaxanthin in E. coli
    Depositor
    Francis X Cunningham Jr
    Article
    Sun et al J Biol Chem. 1996 Oct 4;271(40):24349-52
    Insert
    crtE, crtY, crtI, crtB, crtZ
    Use
    Low copy number bacterial cloning vector
    Tags
    Expression
    Mutation
    Deleted idi (ORF6) and crtX genes
    Promoter
    endogenous promoters
    Available Since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53274-plasmid-map-sequence-id-83289

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  24. PAGFP_NS2B3

    Plasmid
    #195755
    Purpose
    Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
    Depositor
    Partha Mondal
    Article
    Varghese et al bioRxiv 2022.12.18.520514
    Insert
    Dengue (Type-2) NS2B3
    Use
    Tags
    PA-GFP
    Expression
    Mammalian
    Mutation
    No mutations
    Promoter
    CMV
    Available Since
    Feb. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    195755-plasmid-map-sequence-id-384111

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  30. pAD-LyseR (in lacZ deficient strain)

    Plasmid
    #99245
    Purpose
    For autolysate production in lacZ deficient (lac operon deletion) E. coli BL21-Gold-dLac (DE3) strain: constitutively expresses phage lambda endolysin (gene R)
    Depositor
    Jeff Hasty
    Article
    Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102
    Insert
    phage lambda gene R (R phage lambda)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    beta-lactamase promoter
    Available Since
    Oct. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    99245-plasmid-map-sequence-id-191039

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Showing: 6481 - 6510 of 9400 results