165,214 results

1. MCP/PCP RNA Lantern

   Plasmid

   #226095

   Purpose

   RNA tracking

   Depositor

   Jennifer Prescher

   Article

   Halbers et al Nat Commun. 2024 Nov 18;15(1):9992. doi:

   Insert

   NLS-HA-MS2-SmBiT-IRES-PP7-LgBiT-FLAG
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV2/7

   Plasmid

   #112863

   Purpose

   AAV packaging plasmid expressing Rep/Cap genes

   Depositor

   James M. Wilson

   Insert

   Rep2/Cap7
   

   Use

   AAV

   Promoter

   Truncated P5

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Gamillus/ pcDNA3

   Plasmid

   #124837

   Purpose

   mammalian expression of Gamillus, an acid-tolerant monomeric green fluorescent protein

   Depositor

   Takeharu Nagai

   Article

   Shinoda et al Cell Chem Biol. 2018 Mar 15;25(3):330-33

   Insert

   Gamillus
   

   Tags

   No tag

   Expression

   Mammalian

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. SpCas9-HF1

   Plasmid

   #138556

   Purpose

   Expresses human codon-optimized high-fidelity SpCas9 and blasticidin resistance: EFS promoter-SpCas9-HF1-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   SpCas9-HF1
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. VPS13D^Halo

   Plasmid

   #174108

   Purpose

   The insert is a codon optimized DNA sequence encoding VPS13D with an internal Halo tag.

   Depositor

   Pietro De Camilli

   Article

   Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

   Insert

   Codon optimized VPS13D
   

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   With an internal Halo tag in aminoacid position 1…

   Promoter

   CMV

   Available Since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. GPR161-Tango

   Plasmid

   #66334

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   GPR161 (GPR161 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pBGPa-CMV-GFP-OSBP PH domain

   Plasmid

   #58724

   Purpose

   Expresses GFP-Tagged OSBP PH domain in mammalian cells

   Depositor

   Tim Levine

   Article

   Levine et al Curr Biol. 2002 Apr 30;12(9):695-704.

   Insert

   Oxysterol-binding Protein PH domain (OSBP Human)
   

   Tags

   GFP and myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

8. PGKp-GFP-YAP

   Plasmid

   #174172

   Purpose

   Lentiviral vector to constitutively express YAP under control of the human PGK promoter.

   Depositor

   Rod Bremner

   Article

   Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

   Insert

   YAP (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   3x FLAG tag

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Aug. 19, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pNK497

   Plasmid

   #219754

   Purpose

   MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
   

   Expression

   Plant

   Available Since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. PCMV-intron myc Rab2 S20N

    Plasmid

    #46780

    Purpose

    expression of S20N Rab2a in mammalian cells

    Depositor

    Terry Hébert

    Article

    Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

    Insert

    RAB2 (RAB2A Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    S20N dominant negative

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

11. spNW100

    Plasmid

    #173488

    Purpose

    Expresses spNW100 in Bl21

    Depositor

    Huan Bao

    Article

    Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

    Insert

    spNW100
    

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    P1049L- please see depositor comments

    Promoter

    T7

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. tet-TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA

    Plasmid

    #221235

    Purpose

    Tet-inducible lentiviral HACE Editor as a fusion of TadA-8e adenosine deaminase and an optimized PcrA M6 helicase.

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA
    

    Use

    Lentiviral

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLX304-IRES-GFP

    Plasmid

    #176288

    Purpose

    pLX304 backbone with an IRES-GFP cassette subcloned into the NheI-AgeI sites

    Depositor

    William Kaelin

    Article

    Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJR98

    Plasmid

    #187239

    Purpose

    CR3 constant region - hU6 sgRNA promoter flanked by BsmBI sites

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    CR3 constant region - hU6 sgRNA promoter flanked by BsmBI sites
    

    Expression

    Bacterial

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. qTAG-C-mStayGold-Zeo

    Plasmid

    #227255

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHDR-USR-SpCas9

    Plasmid

    #208829

    Purpose

    HDR Universal Surrogate Reporter with a sgRNA and consistently expressed SpCas9. The PuroR reporter gene was designed to be repaired as a selecting marker gene only by the sgRNA/Cas9-triggered HDR.

    Depositor

    Zhiying Zhang

    Article

    Yan et al Mol Ther Nucleic Acids. 2020 Mar 6;19:77

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. SPDK3888(TRV-RNA2-pPEBV-MCS-AtIleu-tRNA)

    Plasmid

    #149276

    Purpose

    Modified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAs

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence
    

    Use

    T-dna vector

    Mutation

    T2318C (DNA) in PEBV coat protein sequence

    Available Since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. IronFistMUT.

    Plasmid

    #243008

    Purpose

    Iron binding deficient IronFist

    Depositor

    Roland Malli

    Article

    Akyol et al ACS Sens. 2025 Aug 22;10(8):5854-5861. d

    Inserts

    hemerythrin-like-mNeonGreen (FBXL5 Human)
    T2A
    mCherry
    

    Use

    Gateway

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    H15A, H57A, E58A, E61A, H126A, E130A

    Promoter

    human phosphoglycerate kinase (hPGK)

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. Iron Insensitive Control Construct 2

    Plasmid

    #243007

    Purpose

    Iron Insensitive Control Construct under the same expression promoter used in IronFist

    Depositor

    Roland Malli

    Article

    Akyol et al ACS Sens. 2025 Aug 22;10(8):5854-5861. d

    Inserts

    mNeonGreen
    T2A
    mCherry
    

    Use

    Gateway

    Expression

    Mammalian

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. IronFist

    Plasmid

    #243006

    Purpose

    Genetically encoded fluorescent iron reporter

    Depositor

    Roland Malli

    Article

    Akyol et al ACS Sens. 2025 Aug 22;10(8):5854-5861. d

    Inserts

    Hemerythrin-like (FBXL5 Human)
    T2A
    mCherry
    

    Use

    Gateway

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    human phosphoglycerate kinase (hPGK)

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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21. AAV-CMV-GFP

    Plasmid

    #67634

    Purpose

    AAV vector expressing GFP

    Depositor

    Connie Cepko

    Article

    Xiong et al J Clin Invest. 2015 Apr;125(4):1433-45.

    Insert

    GFP
    

    Use

    AAV

    Promoter

    CMV

    Available Since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAAV-TRE-NpHR-EYFP

    Plasmid

    #171621

    Purpose

    TRE-NpHR-EYFP reporter

    Depositor

    Hyungbae Kwon

    Article

    Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

    Insert

    TRE-NpHR-EYFP
    

    Use

    AAV and Synthetic Biology

    Expression

    Mammalian

    Promoter

    TetO

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCW57.1_AOX-FLAG

    Plasmid

    #177984

    Purpose

    All-in-one dox-inducible expression of Alternative Oxidase

    Depositor

    Jessica Spinelli, David Sabatini

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    AOX
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Codon optimized cDNA for expression in human cells

    Promoter

    TRE

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. pAG1160 mCherry-FIREtag

    Plasmid

    #205164

    Purpose

    Expresses mCherry -FIREtag in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    mCherry-FIREtag
    

    Expression

    Mammalian

    Available Since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. lentiTet-LDLR-mCherry BlastR

    Plasmid

    #186739

    Purpose

    Dox inducible LDLR coding sequence expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

    Depositor

    Richard Sherwood

    Article

    Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

    Insert

    LDLR (LDLR Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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26. pAAV-hSYN1-fDIO-tTA

    Plasmid

    #166597

    Purpose

    Encodes Flp-dependent tTA

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    tTA
    

    Use

    AAV

    Promoter

    human synapsin 1

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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27. Hsp90 HA

    Plasmid

    #22487

    Depositor

    William Sessa

    Article

    Garcia-Cardena et al Nature. 1998 Apr 23. 392(6678):821-4.

    Insert

    Hsp90 HA (HSP90AB1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    May 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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28. AAV-hSynI-V5-TurboID(N)-GPI

    Plasmid

    #163696

    Purpose

    Expresses n-terminal half of TurboID on surface of neurons

    Depositor

    Scott Soderling

    Article

    Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

    Insert

    GPI anchored TurboID
    

    Use

    AAV

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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29. pEGFP-N1-FLAG

    Plasmid

    #60360

    Purpose

    Plasmid for cloning and expression of FLAG-GFP tagged proteins (C-terminal tag). Confers resistance to G418.

    Depositor

    Patrick Calsou, Sébastien Britton

    Article

    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    EGFP and FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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30. GW1CMV-Perceval

    Plasmid

    #21737

    Purpose

    can be used to monitor the ATP:ADP ratio during live-cell imaging

    Depositor

    Gary Yellen

    Article

    Berg et al Nat Methods. 2009 Feb . 6(2):161-6.

    Insert

    Perceval (fluorescent ATP:ADP sensor)
    

    Tags

    5'UTR

    Expression

    Mammalian

    Mutation

    improved Kozak sequence

    Available Since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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