4,168 results

1. pET-28a_6H-TAQ_E602D

   Plasmid

   #166944

   Purpose

   Bacterial expression of Taq DNA polymerase

   Depositor

   Robert Tjian

   Article

   Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

   Insert

   Thermus aquaticus DNA polymerase
   

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   E602D

   Promoter

   T7

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pACYC-GroEL/ES-TF

   Plasmid

   #83923

   Purpose

   Co-expresses cytoplasmic copies of the Gro EL/ES and Trigger Factor protein folding chaperones.

   Depositor

   Karl Griswold

   Article

   Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

   Inserts

   Gro EL/ES
   Trigger Factor
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Ef1a-DIO eNpHR 3.0-EYFP

   Plasmid

   #26966

   Purpose

   AAV expression of eNpHR 3.0 fused to EYFP driven by EF1a promoter for optogenetic inhibition

   Depositor

   Karl Deisseroth

   Article

   Gradinaru et al Cell. 2010 Apr 2. 141(1):154-65.

   Has Service

   AAV Retrograde, AAV1, AAV5, and AAV9

   Insert

   eNpHR 3.0
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   ER export at 3' end of gene. Trafficking sig…

   Promoter

   EF1alpha

   Available Since

   Jan. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-FLEX-TeLC-P2A-dTomato

   Plasmid

   #159102

   Purpose

   Cre-dependent tetanus toxin light chain construct with nuclear-localized dTomato fluorophore for conditional silencing of neurons.

   Depositor

   Sandeep Datta

   Article

   Pashkovski et al Nature. 2020 Jul;583(7815):253-258. doi:

   Insert

   TeLC-P2A-NLS-dTomato
   

   Use

   AAV

   Promoter

   hSynapsin

   Available Since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. HA-hULK1

   Plasmid

   #31963

   Depositor

   Do-Hyung Kim

   Article

   Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

   Insert

   ULK1 (ULK1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV, SP6

   Available Since

   Sept. 1, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV_ABEmax_P2A_GFP

   Plasmid

   #112101

   Purpose

   A:T-to-G:C base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

   Insert

   ABEmax_P2A_GFP
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmGFP-P2A-K0-P2A-RFP

   Plasmid

   #105686

   Purpose

   Double fluorescent stalling reporter K0

   Depositor

   Ramanujan Hegde

   Article

   Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

   Inserts

   P2A-3xFLAG-VHPbeta
   K0
   P2A-mCherry
   

   Expression

   Mammalian

   Promoter

   CMV (from vector)

   Available Since

   Feb. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSCV-XZ066-EGFRvIII

   Plasmid

   #20737

   Depositor

   Alonzo Ross

   Article

   Li et al Neuro Oncol. 2009 Feb . 11(1):9-21.

   Insert

   EGFRvIII (EGFR Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   EGFR with a 267 amino acid deletion in the extrac…

   Available Since

   March 31, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-GFP-LC3-RFP-LC3ΔG

   Plasmid

   #168997

   Purpose

   Expresses GFP-LC3-RFP-LC3ΔG in mammalian cells and zebrafish to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Tags

   EGFP and mRFP1

   Expression

   Mammalian

   Available Since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

    Plasmid

    #138147

    Purpose

    Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    CasRx
    

    Use

    Lentiviral

    Tags

    NLS

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. psPAX2-D64V

    Plasmid

    #63586

    Purpose

    psPAX2-D64V is derived from psPAX2 plasmid. It is a packaging plasmid for making Integrase deficient lentiviral vectors since has a point mutation in the integrase gene.

    Depositor

    Andrew Scharenberg, David Rawlings

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    Packaging plasmid for integrase deficient lentiviral vectors
    

    Use

    Integrase deficient lentiviral packaging vector

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC013 - Twinstrep-SUMO-huLwCas13a

    Plasmid

    #90097

    Purpose

    Twinstrep-SUMO-huLwCas13a for recombinant protein bacterial expression. Insert is human codon optimized but expresses well in bacteria.

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

    Insert

    LwCas13a
    

    Use

    CRISPR

    Tags

    6xHis-Twin Strep-SUMO

    Expression

    Bacterial

    Available Since

    June 13, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. Lenti-luciferase-P2A-Neo

    Plasmid

    #105621

    Purpose

    3rd generation lentiviral plasmid for expression of luciferase with Neo resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    Luciferase
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST53-LRRK2-WT

    Plasmid

    #25044

    Depositor

    Mark Cookson

    Article

    Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

    Insert

    LRRK2 (LRRK2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    June 11, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSP2N2

    Plasmid

    #29520

    Depositor

    Stephen Sligar

    Article

    Grinkova et al Protein Eng Des Sel. 2010 Nov . 23(11):8

    Insert

    MSP2N2
    

    Tags

    7-His tag

    Expression

    Bacterial

    Mutation

    tandem MSP; His-tag on N-terminus followed by spa…

    Available Since

    May 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. dCas13d-dsRBD-APEX2

    Plasmid

    #154939

    Purpose

    TetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFP

    Depositor

    Alice Ting

    Article

    Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

    Insert

    TetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFP
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. TfR-pHuji

    Plasmid

    #61505

    Purpose

    Human transferrin receptor tagged with pH sensitive red fluorescent protein pHuji

    Depositor

    David Perrais

    Article

    Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

    Insert

    Transferrin receptor (TFRC Human)
    

    Tags

    pHuji

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-TORCAR

    Plasmid

    #64927

    Purpose

    FRET-based biosensor for monitoring mTORC1 kinase activity.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    TORCAR (EIF4EBP1 Human, Synthetic)
    

    Tags

    Cerulean and YPet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCW57-GFP-2A-MCS

    Plasmid

    #71783

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Expression

    Mammalian

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET_mNG2(1-10)_32aalinker_mNG2(11)

    Plasmid

    #82611

    Purpose

    Bacterial expression vector for split mNeonGreen2 screening

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    mNeonGreen2(1-10)_32aalinker_mNeonGreen2(11)
    

    Expression

    Bacterial

    Mutation

    split before mutations

    Promoter

    T7

    Available Since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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