20,036 results
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Plasmid#135003PurposeLentiviral vector to express mCherry or to clone and express gene of interest, under control of EF1a promoter. Barcodes compatible with detection in single cell RNA-seq may be inserted at HpaI site.DepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterEF1aAvailable SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only
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pMF440
Plasmid#62550PurposeBroad host range plasmid for constitutive expression of mCherry. Designed for expression in Pseudomonas aeruginosa.DepositorInsertmCherry
ExpressionBacterialPromotertrcAvailable SinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
v5 Cas9-eVLP
Plasmid#228468PurposeExpresses MMLVgag(C507V)–Cas9 for producing v5 Cas9-eVLPsDepositorInsertMMLVgag(C507V)–Cas9
TagsFLAGExpressionMammalianMutationMMLVgag(C507V)PromoterCMVAvailable SinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTUB1:YFP-mAID-3HA, DHFR-TS:HXGPRT
Plasmid#87259PurposeYFP-mAID-3HA fusion driven by a minimal TUB1 promoter with an HXGdrug selectable marker. The mAID CDS is from Arabidopsis thaliana auxin-responsive protein IAA17E66-S133DepositorInsertsYFP-mAID-3HA
HXGPRT
UseToxoplasma expressionTags3HA and mAIDMutationCodon optimized for Toxoplasma gondii expressionPromoterTgDHFR-TS 463 bp and TgTUB1 378 bpAvailable SinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGH125_dCas9-Blast
Plasmid#85417Purposelentiviral expression vector for dCas9 with Blasticidin selectable markerDepositorInsertdCas9 and Blasticidin resistance
UseCRISPR and LentiviralExpressionMammalianAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pN1/G3BP1-iRFP
Plasmid#129339PurposeStress granule markerDepositorInsertiRFP (from Dr. M. Matsuda)
ExpressionMammalianAvailable SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRU1097
Plasmid#14462DepositorInsertgfp-mut3.1
ExpressionBacterialAvailable SinceApril 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-NE2m
Plasmid#208686PurposeExpresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m in neuronsDepositorInsertGPCR activation based norepinephrine (NE) sensor GRAB_NE2m
UseAAVPromoterhSynAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-RNaseHI-NES-EGFP
Plasmid#196701PurposePlasmid for stable expression of wild type bacterial RNase HI tagged with NES and EGFP that can be used to specifically degrade the DNA-RNA hybrids in the cytoplasm.DepositorInsertbacterial RNaseHI
UseLentiviralAvailable SinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-rtTA
Plasmid#175274PurposeAAV vector mediating neuron-specific expression of rtTADepositorHas ServiceAAV1InsertReverse tetracycline-controlled transactivator (rtTA)
UseAAVPromoterSynapsinAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
CP1041-ABE8e
Plasmid#138493PurposeA-to-G base editorDepositorInsertecTadA(8e)-nSpCas9 (CP1041)
ExpressionMammalianAvailable SinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCGN-Oct-1
Plasmid#53308Purposeplasmid contains POU2F1 (Oct-1) gene with HA-tagDepositorAvailable SinceMay 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1_hTRIM32
Plasmid#69541PurposeExpresses human TRIM32 fused to c-terminal EGFPDepositorAvailable SinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAJM.773
Plasmid#108527PurposeVanRAM + PVanCC-YFP Reporter. Van sensor for use in non-Marionette strain.DepositorInsertVanRAM + PVanCC-YFP
ExpressionBacterialAvailable SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-CMV-GFP-FLAG-cGAS
Plasmid#86675PurposeExpresses catalytically-active GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.DepositorInsertGFP-FLAG-cGAS
UseLentiviralAvailable SinceApril 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgControl
Plasmid#125836PurposeKO controlDepositorInsertControl
UseLentiviralAvailable SinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTS117 His14-Avi-45xGS-anti GFP nanobody
Plasmid#199370PurposeBacterial expression plasmid for anti-GFP nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)DepositorInsertanti-GFP nanobody
Tags14xHis-AviExpressionBacterialMutationWTPromoterT5-LacOAvailable SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
p414-TEF1p-Cas9-CYC1t
Plasmid#43802PurposeA human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the TEF1 promoterDepositorArticleInsertHuman Optimized S. pyogenes Cas9
UseCRISPRExpressionYeastPromoterTEF1 promoterAvailable SinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHR-tdMCP-YFP
Plasmid#99151PurposeExpresses dimeric MS2-coat binding protein tagged with YFP, 2nd generation lentiviral vectorDepositorInsertDimeric MS2-coat binding protein
UseLentiviralTagseYFPExpressionMammalianPromoterSFFVAvailable SinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-CD19.8H.8TM.BB.3z
Plasmid#200671PurposeModular CD8hinge-CD8transmembrane-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsMycTagExpressionMammalianPromoterEF1a-shortAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only