Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 5,795 results for: plasmid dna

Showing: 1051 - 1100 of 5795 results

  6. pcDNA3 H2B-mIFP T2A Mpro (C145A)

    Plasmid
    #163080
    Purpose
    Expresses inactive Mpro mutant and a near-infrared fluorescent protein mIFP fused to H2B
    Depositor
    Xiaokun Shu
    Insert
    Inactive Mpro mutant C145A and H2B-mIFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    163080-plasmid-map-sequence-id-320827

    Enlarge

  7. pcDNA3-mem-TurboID

    Plasmid
    #149409
    Purpose
    Mammalian expression of secretory pathway membrane-localized TurboID, N-term HA tag, C-term V5 tag, in mammalian expression plasmid. Amp/Neo selection
    Depositor
    Jonathan Long
    Article
    Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d
    Insert
    mem-TurboID
    Use
    Tags
    HA and V5
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Nov. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    149409-plasmid-map-sequence-id-299724

    Enlarge

  20. pcDNA3-Nrxn1beta AS4(-)-Fc

    Plasmid
    #59313
    Purpose
    Expression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(-) [lacking the insert at alternatively spliced segment 4 (AS4-)]
    Depositor
    Peter Scheiffele, Tito Serafini
    Article
    Scheiffele et al Cell. 2000 Jun 9. 101(6):657-69.
    Insert
    Nrxn1beta AS4(-)-Fc fusion (Nrxn1 Mouse)
    Use
    Tags
    Fc (from human IgG)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    June 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    59313-plasmid-map-sequence-id-107095

    Enlarge

  21. pcDNA3-Nrxn1beta AS4(+)-Fc

    Plasmid
    #59314
    Purpose
    Expression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(+) [containing the insert at alternatively spliced segment 4 (AS4+)]
    Depositor
    Peter Scheiffele, Tito Serafini
    Article
    Scheiffele et al Cell. 2000 Jun 9. 101(6):657-69.
    Insert
    Nrxn1beta AS4(+)-Fc fusion (Nrxn1 Mouse)
    Use
    Tags
    Fc (from human IgG)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    June 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    59314-plasmid-map-sequence-id-107094

    Enlarge

  24. pcDNA3.1(+)Gαi1-91-NLuc

    Plasmid
    #207148
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI1 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2
    Depositor
    Tom Van Loy, Dominique Schols
    Article
    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76
    Insert
    GNAI1 (GNAI1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    NLuc inserted at position L(91)K
    Promoter
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207148-plasmid-map-sequence-id-410277

    Enlarge

  39. pcDNA3.1-IARS2-H112A/P115A

    Plasmid
    #232338
    Purpose
    Expresses human IARS2 with putative isoleucine binding deficiency mutations (H112A and P115A) in mammalian cells, also carrying synonymous mutations described in Addgene #232340
    Depositor
    Quanfu Ma
    Insert
    IARS2 (IARS2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Putative isoleucine binding deficiency mutation H…
    Promoter
    CMV
    Available Since
    June 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    232338-plasmid-map-sequence-id-474537

    Enlarge

  40. pcDNA3.1-IARS2-K664A/K667A

    Plasmid
    #232339
    Purpose
    Expresses human IARS2 with putative tRNA binding deficiency mutations (K664A and K667A) in mammalian cells, also carrying synonymous mutations described in Addgene #232340
    Depositor
    Quanfu Ma
    Insert
    IARS2 (IARS2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Putative tRNA binding deficiency mutation K664A A…
    Promoter
    CMV
    Available Since
    June 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    232339-plasmid-map-sequence-id-474533

    Enlarge

  41. pcDNA3.1-H2-18 Heavy chain-SpyTag003

    Plasmid
    #216307
    Purpose
    Expresses anti-HER2 H2-18 Heavy chain-SpyTag003 in mammalian cells. To be paired with H2-18 light chain to form the anti-HER2 H2-18 Fab
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    H2-18 Heavy chain-SpyTag003
    Use
    Affinity Reagent/ Antibody
    Tags
    His6 tag, Signal peptide, and SpyTag003
    Expression
    Mammalian
    Mutation
    Promoter
    T7 promoter
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216307-plasmid-map-sequence-id-433370

    Enlarge

  45. pcDNA3.1(+)Gαi3-91-NLuc

    Plasmid
    #207150
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI3 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2
    Depositor
    Tom Van Loy, Dominique Schols
    Article
    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76
    Insert
    GNAI3 (GNAI3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    NLuc inserted at position L(91)K
    Promoter
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207150-plasmid-map-sequence-id-410284

    Enlarge

  47. pcDNA3.1(+)Gαi2-91-NLuc

    Plasmid
    #207149
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI2 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2
    Depositor
    Tom Van Loy, Dominique Schols
    Article
    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76
    Insert
    GNAI2 (GNAI2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    NLuc inserted at position L(91)Q
    Promoter
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207149-plasmid-map-sequence-id-410273

    Enlarge

Showing: 1051 - 1100 of 5795 results