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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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164,127 results

Showing: 8761 - 8800 of 164127 results

  1. mApple-LAMP1-phLuorin-N-8

    Plasmid
    #54918
    Purpose
    Localization: Lysosome Membrane, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    LAMP1 phLuorin
    Use
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    Lysosomes-20 double labeled with phLuorin
    Promoter
    CMV
    Available Since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    54918-plasmid-map-sequence-id-372787

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  7. pCAGIG

    Plasmid
    #11159
    Purpose
    Mammalian expression vector for cloning and expressing your gene on a bicistronic mRNA also encoding IRES-GFP
    Depositor
    Connie Cepko
    Article
    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10
    Has Service
    Cloning Grade DNA
    Insert
    IRES-EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    April 13, 2006
    Availability
    Academic Institutions and Nonprofits only
    11159-plasmid-map-sequence-id-275611

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  9. pCSN068

    Plasmid
    #101749
    Purpose
    Single copy yeast plasmid expressing Cpf1 from Francisella novicida U112 (FnCpf1), codon optimized for expression in Saccharomyces cerevisiae.
    Depositor
    Rene Verwaal
    Article
    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278
    Inserts
    Cpf1 from Francisella novicida U112 (FnCpf1) codon optimized for expression in S. cerevisiae.
    KanMX marker expression cassette.
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Mutation
    Promoter
    Heterologous TEF1 promoter from A. gossypii. and …
    Available Since
    Jan. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    101749-plasmid-map-sequence-id-202306

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  10. pLKO-FOXM1-shRNA-84

    Plasmid
    #89494
    Purpose
    Lentiviral vector with constitutive expression of shRNA targeting human FOXM1.
    Depositor
    Adam Karpf
    Insert
    FOXM1 (FOXM1 Human)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available Since
    June 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    89494-plasmid-map-sequence-id-180323

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  12. pVC-FUS-WT

    Plasmid
    #175066
    Purpose
    expresses mVenus and mCherry tagged to FUS wildtype in mammalian cells
    Depositor
    Arnold Boersma
    Article
    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018
    Insert
    FUS glycine rich protein (FUS Human)
    Use
    Tags
    mVenus + mCherry
    Expression
    Mammalian
    Mutation
    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…
    Promoter
    CMV Promoter
    Available Since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175066-plasmid-map-sequence-id-372335

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  16. pAAV EF1a DIO iChloC 2A dsRed

    Plasmid
    #70762
    Purpose
    An improved chloride-conducting channelrhodopsin for light-induced inhibition of neuronal activity in vivo. Cre inducible expression (double floxed inversed ORF)
    Depositor
    Thomas Oertner
    Article
    Wietek et al Sci Rep. 2015 Oct 7;5:14807. doi: 10.103
    Inserts
    Channelrhodopsin-2
    red fluorescent protein
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    E83Q,E90R,E101S,D156N,T159C
    Promoter
    EF1a
    Available Since
    Jan. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    70762-plasmid-map-sequence-id-134333

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  18. pCBC-MT1T2

    Plasmid
    #50593
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Insert
    T1, gRNA scaffold, OsU3t plus, TaU3p, T2
    Use
    CRISPR; Pcr template
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50593-plasmid-map-sequence-id-451910

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  25. pETDuet-TFIIF

    Plasmid
    #171082
    Purpose
    Co-expresses human TFIIF. The resulting plasmid was used to generate a expression construct encoding 2 subunits, with His-tag on RAP30. Originally from MN Gonzalez, was submitted with permission
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Inserts
    RAP30 (GTF2F2 Human)
    RAP74 (GTF2F1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available Since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171082-plasmid-map-sequence-id-335551

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  28. pUV15-pHGFP

    Plasmid
    #70045
    Purpose
    encodes a pH-sensitive ratiometric GFP (pH-GFP), which allows for noninvasive measurements of intrabacterial pH in live cells
    Depositor
    Sabine Ehrt
    Article
    Vandal et al Nat Med. 2008 Aug;14(8):849-54. doi: 10.
    Insert
    phGFP
    Use
    Mycobacterium tuberculosis expression vector
    Tags
    Expression
    Mutation
    Promoter
    mycobacterial promoter Psmyc
    Available Since
    Oct. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    70045-plasmid-map-sequence-id-488289

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  36. AAV pCAG-FLEX-mScarlet-WPRE

    Plasmid
    #99280
    Purpose
    Can be used to generate AAV virus that will express mScarlet in the presence of Cre
    Depositor
    Rylan Larsen
    Insert
    mScarlet
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    99280-plasmid-map-sequence-id-487711

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Showing: 8761 - 8800 of 164127 results