165,214 results

1. pT3TS-RfxCas13d-NLS-HA

   Plasmid

   #141321

   Purpose

   Plasmid to carry out IVT of RfxCas13d-NLS (human codon-optimized)

   Depositor

   Miguel Angel Moreno-Mateos, Ariel Bazzini

   Article

   Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

   Insert

   Rfx-Cas13d (NLS-RfxCas13d-NLS)
   

   Use

   CRISPR

   Tags

   HA and NLS

   Available Since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. VPS13B^Halo

   Plasmid

   #224584

   Purpose

   codon optimized Halo tagged VPS13B for expression in mammalian cells

   Depositor

   Pietro De Camilli

   Article

   Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

   Insert

   VPS13B^Halo (VPS13B Human)
   

   Tags

   Halo

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFA6a-GST-TRP1

   Plasmid

   #41603

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   TRP1 (TRP1 Budding Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   GST and yeast ADH1 terminator

   Available Since

   Dec. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-KASH2

   Plasmid

   #187017

   Purpose

   Inducible disruption of LINC Complex

   Depositor

   Jan Lammerding

   Article

   Earle et al Nat Mater. 2019 Dec 16. pii: 10.1038/s41

   Insert

   KASH2
   

   Tags

   eGFP

   Expression

   Mammalian

   Available Since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. lenti-NLS-miRFP670nano-miRFP670

   Plasmid

   #216131

   Purpose

   Lentiviral vector for expressing a fusion of miRFP670nano and miRFP670 localizing to the nucleus.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

   Insert

   EF1a-NuclearLocalizationSignal-miRFP670nano-miRFP670-WPRE
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Keratin 8 (pcDNA3)

   Plasmid

   #18063

   Depositor

   M Bishr Omary

   Article

   Ku et al J Biol Chem. 2002 Mar 29. 277(13):10775-

   Insert

   Keratin 8 (KRT8 Human)
   

   Expression

   Mammalian

   Available Since

   May 16, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWZL Neo Myr Flag YES1

   Plasmid

   #20665

   Depositor

   Jean Zhao, William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   YES1 (YES1 Human)
   

   Use

   Retroviral

   Tags

   Flag and Myr

   Expression

   Mammalian

   Available Since

   Oct. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

8. pFA6a-GBP-RFP-kanMX6

   Plasmid

   #105160

   Purpose

   Yeast gene targeting

   Depositor

   Jian-Qiu Wu

   Article

   Davidson et al Mol Biol Cell. 2015 Feb 1;26(3):453-66.

   Insert

   GBP-RFP-kanMX6
   

   Tags

   GBP-RFP

   Expression

   Bacterial and Yeast

   Available Since

   Feb. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGW-GOI-TurboID-GFP

   Plasmid

   #209379

   Purpose

   Gateway compatible plasmid for TurboID-based proximity labelling experiment in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Type

   Empty backbone

   Expression

   Plant

   Mutation

   TurboID gene sequence C249A

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET11b-MIF

    Plasmid

    #149446

    Purpose

    Expression of WT MIF in E. coli for protein production

    Depositor

    Elias Lolis

    Insert

    MIF (MIF Human)
    

    Expression

    Bacterial

    Mutation

    WT

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-Hb9-RFP

    Plasmid

    #37081

    Depositor

    Fred Gage

    Article

    Marchetto et al Cell Stem Cell. 2008 Dec 4;3(6):649-57.

    Insert

    Hb9 RFP
    

    Use

    Lentiviral

    Promoter

    Hb9

    Available Since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGB 35S:Renilla:Tnos (GB0109)

    Plasmid

    #68215

    Purpose

    Transcriptional unit for Renilla Luciferase Protein plant expression driven by the 35S promoter

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Renilla
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available Since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. PX856

    Plasmid

    #62888

    Purpose

    C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64)

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa536-1368)
    

    Use

    CRISPR

    Tags

    FKBP12, NLS, NLS SV40, and VP64

    Expression

    Mammalian

    Mutation

    Asparagine 863 to Alanine (N863A)

    Promoter

    CBh

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCS2+ H2B-mTagBFP2

    Plasmid

    #99267

    Purpose

    Contains a fusion of human histone H2B to fluorescent protein mTagBFP2 in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.

    Depositor

    Philipp Keller

    Insert

    mTagBFP2-3XHA
    

    Tags

    H2B

    Expression

    Mammalian

    Promoter

    SP6

    Available Since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. H6GB1tevP53SS(1-393)

    Plasmid

    #200311

    Purpose

    Bacterial expression of human superstable p53 as His6GB1tev fusion

    Depositor

    Peter Wright

    Article

    Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

    Insert

    p53 (TP53 Human)
    

    Tags

    His6-GB1-tev

    Expression

    Bacterial

    Mutation

    M133L, V203A, N239Y, N268D

    Promoter

    T7

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZC06 (pFA6a-PSc-6xGly-PrtA-KanMX)

    Plasmid

    #44090

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    6xGly linker, ADH1 transcription terminator, KanM…

    Available Since

    April 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTXB1-CfaC-Gyra

    Plasmid

    #122950

    Purpose

    Bacterial expression of C-intein

    Depositor

    Thomas Muir

    Article

    Stevens et al J Am Chem Soc. 2016 Feb 24;138(7):2162-5

    Insert

    CfaC
    

    Expression

    Bacterial

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. Cx36-GCaMP

    Plasmid

    #123604

    Purpose

    Mouse Connexin 36-GCaMP3 fusion for measuring gap junction localized calcium signaling - expresses in mammalian cells

    Depositor

    John O'Brien

    Article

    Moore et al eNeuro. 2020 Mar 11. pii: ENEURO.0445-19

    Insert

    Connexin 36-GCaMP3 fusion (Gjd2 Mouse)
    

    Tags

    GCaMP3

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP

    Plasmid

    #50946

    Purpose

    An empty gRNA expression vector

    Depositor

    Kosuke Yusa

    Article

    Koike-Yusa et al Nat Biotechnol. 2014 Mar;32(3):267-73. d

    Insert

    None
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. N8his-GFPenhancer-GGGGS4-LaG16

    Plasmid

    #140442

    Purpose

    We designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.

    Depositor

    Motoyuki Hattori

    Article

    Zhang et al Sci Rep. 2020 Apr 10;10(1):6239. doi: 10

    Insert

    GFPenhancer-GGGGS4-LaG16
    

    Tags

    8 histidine tag

    Expression

    Bacterial

    Promoter

    T7 promotor

    Available Since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCap-101-Hgy

    Plasmid

    #227508

    Purpose

    Capture plasmid with hygromycin resistance and pIJ101 replicon

    Depositor

    Chengzhang Fu

    Article

    Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

    Type

    Empty backbone

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBbA6c-RFP

    Plasmid

    #35290

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    LlacO-1

    Available Since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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23. pAAV-TRE-DIO-ChR2-TdTomato

    Plasmid

    #166600

    Purpose

    Encodes Cre-dependent ChR2-TdTomato under control of the TRE

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    ChR2-TdTomato
    

    Use

    AAV

    Mutation

    H134R

    Promoter

    tetracycline response element

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. pBAD33-mf-lon

    Plasmid

    #21867

    Depositor

    Robert Sauer

    Article

    Gur et al Proc Natl Acad Sci U S A. 2008 Oct 21. 1

    Insert

    Lon (Mfl404 Mesoplasma florum)
    

    Expression

    Bacterial

    Mutation

    all TGA codons changed to TGG codons

    Available Since

    Aug. 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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25. pRel-Hyg

    Plasmid

    #227582

    Purpose

    Release plasmid with Cas9 working cassette, hygromycin resistance, and pSG5 replicon

    Depositor

    Chengzhang Fu

    Article

    Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

    Insert

    Cas9
    

    Use

    CRISPR

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PX855

    Plasmid

    #62887

    Purpose

    N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa 2-535)
    

    Use

    CRISPR

    Tags

    FRB and NES PTK2

    Expression

    Mammalian

    Mutation

    Aspartic acide 10 to Alanine (D10A)

    Promoter

    CBh

    Available Since

    March 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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27. pAAV-Syn-DIO-NaChBac-dTomato

    Plasmid

    #236252

    Purpose

    Cre dependent NaChBac-TdTomato expression

    Depositor

    Janelia Viral Tools

    Article

    Kosse et al Nature. 2024 Dec;636(8041):151-161. doi:

    Insert

    NaChBac-TdTomato
    

    Use

    AAV

    Tags

    TdTomato

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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28. IGF2BP1

    Plasmid

    #155676

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    IGF2BP1 (IGF2BP1 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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29. pcDNA3.3_SOX2

    Plasmid

    #26817

    Depositor

    Derrick Rossi

    Article

    Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

    Insert

    5'UTR-SOX2-3'UTR (SOX2 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 17, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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30. Ornithine transcarbamylase (OTC)

    Plasmid

    #71877

    Purpose

    For mitochondrial unfolded protein response research

    Depositor

    Nicholas Hoogenraad

    Article

    Zhao et al EMBO J. 2002 Sep 2;21(17):4411-9.

    Insert

    OTC (Otc Rat)
    

    Tags

    None

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge