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Showing: 8921 - 8960 of 157564 results

  1. pCMV-tomm20-ECFP-RspANFAST

    Plasmid
    #216348
    Purpose
    targets ECFP-RspANFAST to mitochondrial membrane
    Depositor
    Alison Tebo
    Insert
    tomm20-ECFP-RspANFAST
    Use
    Tags
    tomm20
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216348-plasmid-map-sequence-id-427395

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  2. Limk1_pLVX-EF1-Cry2-mCherry

    Plasmid
    #64368
    Purpose
    lentiviral expression of Link1 and Cry2-mCherry
    Depositor
    Matthieu Piel
    Insert
    Limk1 (LIMK1 Human)
    Use
    Lentiviral
    Tags
    Cry2-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  5. pHR-UCOE-SFFV-Zim3-dCas9-P2A-EGFP

    Plasmid
    #188899
    Purpose
    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element
    Depositor
    Marco Jost, Jonathan Weissman
    Article
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Zim3-dCas9
    Use
    Lentiviral
    Tags
    HA-2xNLS, P2A-GFP, and Zim3 KRAB-NLS fusion
    Expression
    Mutation
    Promoter
    SFFV
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    188899-plasmid-map-sequence-id-374831

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  6. VIPR2-Tango

    Plasmid
    #66521
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    VIPR2 (VIPR2 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. pCMV-Neo-Bam

    Plasmid
    #16440
    Purpose
    Contains two independent transcription units--a CMV promoter/enhancer upstream of a MCS and a HSV thymidine kinase promoter/enhancer upstream of the neomycin resistance gene.
    Depositor
    Bert Vogelstein
    Article
    Baker et al Science. 1990 Aug 24. 249(4971):912-5.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 7, 2008
    Availability
    Academic Institutions and Nonprofits only
    16440-plasmid-map-sequence-id-211955

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  16. epi-BE4max

    Plasmid
    #135975
    Purpose
    Expresses AncBE4max, EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin
    Depositor
    Yongming Wang
    Article
    Qi et al Front Cell Dev Biol. 2020 Sep 25;8:59058
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    135975-plasmid-map-sequence-id-292615

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  18. pcDNA3-Clover

    Plasmid
    #40259
    Purpose
    GFP with robust performance in protein fusions and useful as FRET acceptor for mRuby2 in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap
    Depositor
    Michael Lin
    Article
    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm
    Has Service
    Cloning Grade DNA
    Insert
    Clover GFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV IE
    Available since
    Sept. 24, 2012
    Availability
    Academic Institutions and Nonprofits only
    40259-plasmid-map-sequence-id-215680

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  21. AAV-CamKIIa-jGCaMP8m-WPRE

    Plasmid
    #176751
    Purpose
    AAV-mediated expression of GCaMP8m under the CamKIIa promoter
    Depositor
    Loren Looger
    Has Service
    AAV Retrograde, AAV1, AAV5, and AAV9
    Insert
    jGCaMP8m
    Use
    AAV
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    CamKIIalpha
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    176751-plasmid-map-sequence-id-349058

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  28. BB3cH_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104912
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    104912-plasmid-map-sequence-id-206066

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  35. pEP4 E02S EN2K

    Plasmid
    #20925
    Purpose
    used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Nanog and Klf4
    Depositor
    James Thomson
    Article
    Yu et al Science. 2009 Mar 26.
    Insert
    Oct4 and Sox2; Nanog and Klf4 (SOX2, POU5F1, NANOG, KLF4 Human)
    Use
    Episomal/ebna
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 20, 2009
    Availability
    Academic Institutions and Nonprofits only
    20925-plasmid-map-sequence-id-9761

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  37. ePPEplus

    Plasmid
    #205241
    Purpose
    For plant prime editing in wheat plants or monocotyledons protoplasts
    Depositor
    Zong Yuan
    Article
    Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:
    Insert
    c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Detailed in manuscript
    Promoter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    205241-plasmid-map-sequence-id-408831

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Showing: 8921 - 8960 of 157564 results