We narrowed to 22,191 results for: his

1. hATMS1981A

   Plasmid

   #32300

   Depositor

   Michael Kastan

   Article

   Bakkenist et al Nature. 2003 Jan 30;421(6922):499-506.

   Insert

   ATM (ATM Human)
   

   Use

   Tags

   Flag and His

   Expression

   Mammalian

   Mutation

   S1981A

   Promoter

   Available Since

   Nov. 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDR119

   Plasmid

   #13332

   Depositor

   Martin Gellert

   Article

   Modesti et al EMBO J. 1999 Apr 1. 18(7):2008-18.

   Insert

   DNA ligase IV (LIG4 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pFasBac+GFP-CAMSAP2

   Plasmid

   #59037

   Purpose

   for baculovirus expression of CAMSAP2 in Sf9 cells

   Depositor

   Ron Vale

   Article

   Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

   Insert

   CAMSAP2 (CAMSAP2 Human)
   

   Use

   Tags

   His and eGFP

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Sept. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBMPC3

   Plasmid

   #31367

   Depositor

   Aziz Sancar

   Article

   Ye et al J Biol Chem. 2011 Jul 22;286(29):25891-9

   Insert

   Bmal1 (Bmal1 Mouse)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMT-flp

   Plasmid

   #67274

   Purpose

   Conjugally transferable Flp recombinase plasmid for unmarked mutagenesis

   Depositor

   Mark Liles

   Article

   Hossain et al Biotechnol Rep (Amst). 2015 Aug 28;8:24-

   Insert

   tetA, flp, repA101, oriR101
   

   Use

   Flp recombinase plasmid, temperature sensitive

   Tags

   His-SUMO (flp)

   Expression

   Mutation

   Promoter

   tetA promoter of plasmid pACYC184 origin

   Available Since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. human p53-(94-312)

   Plasmid

   #24866

   Depositor

   Cheryl Arrowsmith

   Article

   Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains aa 94-312.

   Promoter

   Available Since

   Aug. 5, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pBAD-LSSmGFP

   Plasmid

   #186542

   Purpose

   LSSmGFP fluorescent protein (ex/em = 400/510 nm): bacterial expression plasmid

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   LSSmGFP
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

   Promoter

   araB

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28-hTDG

   Plasmid

   #70758

   Purpose

   bacterial expression of human TDG

   Depositor

   Primo Schaer

   Article

   Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

   Insert

   TDG (TDG Human)
   

   Use

   Tags

   6-HIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7/lac operon

   Available Since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCellFree_G03 CYP3A4

   Plasmid

   #67104

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   CYP3A4 (CYP3A4 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRSETA-qmTQ2-ATP

    Plasmid

    #225247

    Purpose

    Bacterial expression of N-terminal His-tagged qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imaging

    Depositor

    Yasushi Okada

    Article

    Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

    Insert

    qmTQ2-ATP
    

    Use

    Tags

    6XHis, T7 tag, XpressTM tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBAD-FOLD6

    Plasmid

    #186547

    Purpose

    FOLD6 "hyperfolder" green fluorescent protein: bacterial expression plasmid

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    FOLD6
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    mGreenLantern-C48S/V68L/C70V/K101E/T105Y/S147P/K1…

    Promoter

    araB

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. human p53-(1-320)

    Plasmid

    #24864

    Depositor

    Cheryl Arrowsmith

    Article

    Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 1-320

    Promoter

    Available Since

    Aug. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

13. Integrin-β6 Headpiece I270C

    Plasmid

    #159638

    Purpose

    Contains integrin beta 6 residues 1–474 with I270C mutation

    Depositor

    Timothy Springer

    Article

    Dong et al Nat Struct Mol Biol. 2014 Dec;21(12):109

    Insert

    Integrin beta 6 headpiece I270C
    

    Use

    Tags

    6x His

    Expression

    Mammalian

    Mutation

    I270C

    Promoter

    Available Since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMC1SG5

    Plasmid

    #31282

    Depositor

    Aziz Sancar

    Article

    Ye et al J Biol Chem. 2011 Jul 22;286(29):25891-9

    Insert

    Cry1 (Cry1 Mouse)
    

    Use

    Tags

    Flag, His, and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

15. SUMO_MT3

    Plasmid

    #200303

    Purpose

    Bacterial expression plasmids for production of recombinant metallothionein 3 containing N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility

    Depositor

    Artur Krężel

    Article

    Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.

    Insert

    metallothionein 3 (MT3 Human)
    

    Use

    Tags

    Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pETDuet RagA-RagC

    Plasmid

    #99641

    Purpose

    Bacterial expression of wildtype Rag GTPase dimer

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET24b-Amg2

    Plasmid

    #199434

    Purpose

    expresses Amg2 in bacterial cells

    Depositor

    Andrew Woolley

    Article

    Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

    Insert

    Amg2
    

    Use

    Tags

    his tag

    Expression

    Bacterial

    Mutation

    L159K mutation

    Promoter

    T7

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET26b-Granzyme A

    Plasmid

    #8823

    Depositor

    Judy Lieberman

    Article

    Beresford et al Proc Natl Acad Sci U S A 1997 Aug 19;94(

    Insert

    Human Granzyme A (GZMA Human)
    

    Use

    Tags

    (His)6 and Enterokinase site

    Expression

    Bacterial

    Mutation

    it mature enzyme.

    Promoter

    Available Since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pST-K

    Plasmid

    #44560

    Purpose

    for ectopic constitutive expression of mycobacterial proteins

    Depositor

    Vinay Nandicoori

    Article

    Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

    Type

    Empty backbone

    Use

    E.coli-mycobacteria shuttle vector

    Tags

    Flag tag and His tag

    Expression

    Bacterial

    Mutation

    Promoter

    UV15 promoter

    Available Since

    Oct. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pST-KiT

    Plasmid

    #44562

    Purpose

    for integrative inducible expression of mycobacterial proteins

    Depositor

    Vinay Nandicoori

    Article

    Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

    Type

    Empty backbone

    Use

    E.coli-mycobacteria shuttle vector

    Tags

    Flag tag and His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET21b-Caspase-3(V266E)

    Plasmid

    #90089

    Purpose

    Expresses caspase 3 V266E mutant in bacterial cells

    Depositor

    Clay Clark

    Article

    Pop et al Biochemistry. 2003 Oct 28;42(42):12311-2

    Insert

    Caspase 3 (CASP3 Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    V266E

    Promoter

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28a-FIP3

    Plasmid

    #74753

    Purpose

    Expressed sfGFP-tagged FIP3 in E. coli for protein production

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    Rab11 family interacting protein 3 (RAB11FIP3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBAD-luxOFP

    Plasmid

    #229945

    Purpose

    Bacterial expression of a de novo designed, FRET-based neoLux1.2-CyOFP1 fusion bioluminescent probe (max. Em=588)

    Depositor

    Andy Yeh

    Article

    Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

    Insert

    luxOFP
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available Since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28_mouse-pro-IL1B

    Plasmid

    #183386

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    pro-IL1B (Il1b Mouse)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. FR_HNF6

    Plasmid

    #31099

    Depositor

    Gerhart Ryffel

    Article

    Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

    Insert

    HNF6 (ONECUT1 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBAD-luxKate

    Plasmid

    #229946

    Purpose

    Bacterial expression of a de novo designed, FRET-based neoLux1.2-mKate2 fusion bioluminescent probe (max. Em=638)

    Depositor

    Andy Yeh

    Article

    Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

    Insert

    luxKate
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available Since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBad-PhoCl2f

    Plasmid

    #164036

    Purpose

    Improved PhoCl variant with faster dissociation rate

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    PhoCl2f
    

    Use

    Tags

    His tag, T7 tag, Xpress Tag

    Expression

    Bacterial

    Mutation

    Promoter

    araBad promotor

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET11a-link-NGFP

    Plasmid

    #52732

    Purpose

    IPTG-inducible expression of link-NGFP negative control for in vivo split GFP assembly assay

    Depositor

    Lynne Regan

    Article

    Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

    Insert

    link-NGFP
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET28_human-Caspase-4_enzymatic-domain

    Plasmid

    #183381

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-4 (AA94-377) (CASP4 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Venus/Cerulean-Synaptopodin-A in pcDNA6

    Plasmid

    #190798

    Purpose

    Imaging of synaptopodin in live cells

    Depositor

    Vivian Tang

    Article

    Morris et al J Cell Biol. 2022 May 2;221(5). pii: 213

    Insert

    Synaptopodin-A (SYNPO Synthetic, Human)
    

    Use

    Tags

    Cerulean, His, Venus, and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. MUNC18a

    Plasmid

    #218888

    Purpose

    Expresses rat MUNC18a in bacteria

    Depositor

    Axel Brunger

    Article

    Leitz et al Nat Protoc. 2024 Jul 2. doi: 10.1038/s41

    Insert

    Munc18a (Stxbp1 Rat)
    

    Use

    Tags

    6 his

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTXTL-T7max-RLuc

    Plasmid

    #188312

    Purpose

    Expression of Renilla luciferase via a T7 promoter in TXTL

    Depositor

    Kate Adamala

    Article

    Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

    Insert

    Renilla luciferase
    

    Use

    Luciferase and Synthetic Biology

    Tags

    8x His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7Max

    Available Since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pEF-ENTR A (696-6)

    Plasmid

    #17427

    Purpose

    Gateway entry vector with EF1-a promoter, V5-His C-term, frame A

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pClneoFH human RASSF1A

    Plasmid

    #37016

    Depositor

    Yutaka Hata

    Article

    Ikeda et al Sci Signal. 2009 Sep 29;2(90):ra59.

    Insert

    rassf1A (RASSF1 Human)
    

    Use

    Tags

    FLAG and His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pOPINS-UBE3C

    Plasmid

    #66711

    Purpose

    Expresses UBE3C HECT domain in E. coli

    Depositor

    David Komander

    Article

    Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

    Insert

    KiAA0010 (UBE3C )
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only

36. pET28b-pchG WT

    Plasmid

    #87107

    Purpose

    expresses PchG WT, the thiazolinyl imine reductase from Pseudomonas aeruginosa

    Depositor

    Audrey Lamb

    Article

    Meneely et al Biochemistry. 2016 Sep 27;55(38):5423-33

    Insert

    pchG WT
    

    Use

    Tags

    his-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGFP-E p27 (CC#631)

    Plasmid

    #15192

    Depositor

    Connie Cepko

    Article

    Dyer et al J Neurosci. 2001 Jun 15. 21(12):4259-71.

    Insert

    p27 (Cdkn1b Mouse)
    

    Use

    Retroviral

    Tags

    Flag and His

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET28_mouse-pro-IL18

    Plasmid

    #183389

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    pro-IL18 (Il18 Mouse)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCKFB1

    Plasmid

    #31280

    Depositor

    Aziz Sancar

    Article

    Ye et al J Biol Chem. 2011 Jul 22;286(29):25891-9

    Insert

    Clock (Clock Mouse)
    

    Use

    Tags

    Flag and His

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

40. pRSET-GCaMP6f

    Plasmid

    #67564

    Purpose

    bacterial expression of a fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    GCaMP6f
    

    Use

    Tags

    HIS-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only