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170,823 results

Showing: 8961 - 9000 of 170823 results

  5. pET21a(+)-HIV RT-6xHis-RBS-prot

    Plasmid
    #159149
    Purpose
    expresses His-tagged HIV reverse transcriptase and untagged HIV protease
    Depositor
    Andrea Pauli
    Article
    Kellner et al Front Mol Biosci. 2022 Apr 1;9:801309. d
    Inserts
    Human immunodeficiency virus 1 (HIV-1) reverse transcriptase
    Human immunodeficiency virus 1 (HIV-1) protease
    Tags
    His
    Expression
    Bacterial
    Mutation
    HIV-1 group M/subtype B – BH10 strain
    Available Since
    Sept. 1, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    159149-plasmid-map-sequence-id-310074

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  6. TFORF3476

    Plasmid
    #144952
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SNAI1 (SNAI1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144952-plasmid-map-sequence-id-404522

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  9. pCDH-EF1-E-'No'Mi-Red-T2A-Puro

    Plasmid
    #246642
    Purpose
    EF-1alpha driven EV reporter E-'No'Mi-R, a re-engineered tetraspanin scaffold tagged with bioluminescent and fluorescent reporter proteins. Nanoluc and Flag tag are removable with TEVp.
    Depositor
    Koen Breyne
    Article
    Obuchi et al J Extracell Vesicles. 2025 Jun;14(6):e70
    Insert
    cleavable E-NoMi-Red
    Use
    Lentiviral
    Tags
    FLAG-Nanoluciferase and mCherry
    Expression
    Mammalian
    Promoter
    EF-1alpha
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    246642-plasmid-map-sequence-id-489517

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  10. VCP-HaloTag Vector

    Plasmid
    #237005
    Purpose
    Express VCP-HaloTag(R) Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    VCP (VCP Human)
    Tags
    HaloTag (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237005-plasmid-map-sequence-id-472452

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  11. pCEP4-M2L

    Plasmid
    #20926
    Purpose
    used in the derivation of human iPS cells using non-integrating episomal vectors; expresses cMyc and Lin28
    Depositor
    James Thomson
    Article
    Yu et al Science. 2009 Mar 26.
    Inserts
    MYC (MYC Human)
    LIN28 (LIN28A Human)
    Use
    Episomal/ebna
    Promoter
    CMV and IRES
    Available Since
    May 20, 2009
    Availability
    Academic Institutions and Nonprofits only
    20926-plasmid-map-sequence-id-9762

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  20. pDEST-CMV-polysite

    Plasmid
    #122843
    Purpose
    Gateway destination vector for mammalian expression without tag, or for adding custom tags by conventional cloning. Distinct multiple cloning sites 5' and 3' of gateway cassette.
    Depositor
    Robin Ketteler
    Article
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Type
    Empty backbone
    Use
    Gateway destination
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    122843-plasmid-map-sequence-id-244374

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  21. pCBh-NLS_dCas13d_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid
    #191797
    Purpose
    vector for encoding a human codon-optimized dead RfxCas13d (dCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103
    Insert
    humanized dCas13d
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    R239A, H244A, R858A, H863A
    Promoter
    CBh, SV40, hU6
    Available Since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    191797-plasmid-map-sequence-id-375579

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  24. PNLM-pcABE

    Plasmid
    #237618
    Purpose
    PNLM-pcABE generated by pre-trained Protein-Nucleic Acid constrained Language Model exhibits high efficiency, a condensed editing window, and a shortened size.
    Depositor
    Xiaohui Zhang
    Article
    Ren et al Nat Commun. 2025 Dec 13;16(1):11207. doi
    Insert
    ecTadA(8e)-nSpCas9
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    deleted amino acids 2-8 and 147-152
    Promoter
    pCMV
    Available Since
    Feb. 6, 2026
    Availability
    Academic Institutions and Nonprofits only
    237618-plasmid-map-sequence-id-495632

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  25. YY171: pPB_NFκB::GFP-GEMINI(A)_TRE::HaloTag-GEMINI(A)_UbC::rtTA3-IRES-PuroR

    Plasmid
    #228883
    Purpose
    PiggyBac plasmid expressing the GFP-GEMINI(A) under the control of a synthetic NFκB-responsive promotor, and rtTA3 and PuroR under the control of a UbC promoter.
    Depositor
    Dingchang Lin
    Article
    Yan et al bioRxiv [Preprint]. 2025 Jul 18:2025.07.
    Insert
    GFP-fused GEMINI(A), HaloTag-fuse GEMINI(A)
    Expression
    Mammalian
    Promoter
    pNFκB; TRE
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    228883-plasmid-map-sequence-id-454808

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  29. pGL4 [luc2P/hEGR1/Hygro] Vector

    Plasmid
    #236821
    Purpose
    Express hEGR1 response element in luciferase reporter assay
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    hEGR1 (EGR1 Human)
    Use
    Luciferase
    Mutation
    None
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236821-plasmid-map-sequence-id-492597

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  36. TRUPATH Triple Ga11

    Plasmid
    #196059
    Purpose
    Encodes a G alpha subunit (GNA11) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlpha11-RLuc8 (GNA11 Human)
    GGamma13-GFP2 (GNG13 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    196059-plasmid-map-sequence-id-386126

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  38. NanoBRET BiBRET Vector MAVS/IFIH1

    Plasmid
    #237022
    Purpose
    Express HaloTag-MAVS/NanoLuc(R)-IFIH1 Fusion Proteins in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Inserts
    MAVS (MAVS Human)
    IFIH1 (IFIH1 Human)
    Tags
    HaloTag (R) and NanoLuc (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237022-plasmid-map-sequence-id-471884

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  40. Gi2-CASE

    Plasmid
    #168121
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi2. Composed of the subunits G alpha i2 (GNAI2) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAI2, GNB1, GNG2 (GNAI2, GNB1, GNG2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG2
    Expression
    Mammalian
    Mutation
    NLuc is inserted at C112/E115 within GNAI2
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168121-plasmid-map-sequence-id-332861

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Showing: 8961 - 9000 of 170823 results