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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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164,127 results

Showing: 8961 - 9000 of 164127 results

  1. pET30-T7-sfGFP

    Plasmid
    #180757
    Purpose
    pET30 vector missing lacO and N-terminal His tag with sfGFP-S-tag inserted between the Bgl II and T7 terminator
    Depositor
    Michael Black
    Article
    Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:
    Insert
    superfolder green fluorescent protein
    Use
    Tags
    strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    July 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    180757-plasmid-map-sequence-id-359679

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  25. pTNS2

    Plasmid
    #87802
    Purpose
    For constructing NusA-SUMO-fusion proteins with N-terminal StrepII tag and C-terminal His tag using "blunt-end" cloning.
    Depositor
    Yu-Zhu Zhang
    Article
    Zhang et al Mol Biotechnol. 2017 May;59(4-5):159-167
    Type
    Empty backbone
    Use
    Tags
    6xHis and Strep II
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87802-plasmid-map-sequence-id-177263

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  32. GAL1-DuET

    Plasmid
    #213248
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GAL1 (GALR1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213248-plasmid-map-sequence-id-430900

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  33. TRIP8b (1b-2)-GFP/pEGFP-C1

    Plasmid
    #197336
    Purpose
    Plasmid expressing an antigen targeting TRIP8b (exon 1b)
    Depositor
    Steve Siegelbaum
    Insert
    TRIP8b (1b-2) (Pex5l Mouse)
    Use
    Tags
    eGFP (GFP observed)
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Jan. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    197336-plasmid-map-sequence-id-401590

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  35. pVITRO1-102.1F10-IgE/λ

    Plasmid
    #50365
    Purpose
    Expresses grass pollen allergen Phl p 7 specific human IgE/λ antibody isotype (102.1F10-IgE/λ)
    Depositor
    Andrew Beavil
    Article
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    grass pollen allergen Phl p 7 specific human epsilon heavy chain expression cassette
    grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Mouse Elongation Factor 1 Alpha and Rat Elongatio…
    Available Since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    50365-plasmid-map-sequence-id-367944

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  38. FLT3-D835Y-MSCV-IRES-GFP

    Plasmid
    #184780
    Purpose
    Expresses FLT3 D835Y activating tyrosine kinase domain mutant in mammalian cells
    Depositor
    Timothy Ley
    Insert
    FLT3 (FLT3 Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Activating tyrosine kinase domain mutation (D835Y)
    Promoter
    MSCV
    Available Since
    July 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    184780-plasmid-map-sequence-id-367916

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Showing: 8961 - 9000 of 164127 results