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Addgene

We narrowed to 22,309 results for: Sis

Showing: 11251 - 11300 of 22309 results
  1. pHelper-CRISPRi-sgRNA

    Plasmid
    #221135
    Purpose
    Helper plasmid for sgRNA cloning for CRISPRi. sgRNA-scaffold with dCas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.
    Depositor
    Insert
    sgRNA-scaffold with dCas9 handle
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119(SpeI)
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. PtetA-PLacO3O1 150 bp with pBELO_L6-PT3-T4_CAMR_TAN+

    Plasmid
    #225645
    Purpose
    tetA and LacO3O1 promoters in tandem conformation with 150bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance
    Insert
    tetR/tetA and LacO3O1 in tandem conformation
    Use
    Tags
    mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    tetA and LacO3O1 promoters
    Available since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. PLacO3O1-PtetA 250 bp with pBELO_L6-PT3-T4_CAMR_TAN+

    Plasmid
    #225648
    Purpose
    LacO3O1 and tetA promoters in tandem conformation with 250bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance
    Insert
    LacO3O1 and tetR/tetA in tandem conformation
    Use
    Tags
    mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    LacO3O1 and tetA promoters
    Available since
    Oct. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. PLacO3O1-PtetA 150 bp with pBELO_L6-PT3-T4_CAMR_TAN+

    Plasmid
    #225647
    Purpose
    LacO3O1 and tetA promoters in tandem conformation with 150bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance
    Insert
    LacO3O1 and tetR/tetA in tandem conformation
    Use
    Tags
    mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    LacO3O1 and tetA promoters
    Available since
    Oct. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. PtetA-PLacO3O1 350 bp with pBELO_L6-PT3-T4_CAMR_TAN+

    Plasmid
    #225646
    Purpose
    tetA and LacO3O1 promoters in tandem conformation with 350bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance
    Insert
    tetR/tetA and LacO3O1 in tandem conformation
    Use
    Tags
    mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    tetA and LacO3O1 promoters
    Available since
    Oct. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. pDUP2

    Plasmid
    #223517
    Purpose
    cogfp copy 1 under control of Ptet, cogfp copy 2 under control of Ptac
    Depositor
    Inserts
    CoGFP
    CoGFP (second copy)
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    anti-dimerization mutations S129G, 22 C154S, D156…
    Promoter
    Ptac, Ptac and Ptet, and Ptet
    Available since
    Sept. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. pET28Man

    Plasmid
    #203399
    Purpose
    exo-beta mannosidase useful in enzymatic biotransformations of glycans and in coupled assays of various carbohydrate processing enzymes
    Depositor
    Insert
    man2A
    Use
    Tags
    H6
    Expression
    Bacterial
    Mutation
    None
    Promoter
    T7
    Available since
    Aug. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
  8. pro::EX1-2(intΔNACs):GFP

    Plasmid
    #218570
    Purpose
    Transcriptional reporter for ARF7 promoter
    Depositor
    Insert
    AT5G20730 (NPH4 Mustard Weed)
    Use
    Tags
    Expression
    Plant
    Mutation
    Mutation genomic sequence 85nt, 86nt from TA to C…
    Promoter
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 11251 - 11300 of 22309 results