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pAAV-Syn1-fDIO-mScarlet-KASH-2a-Cre

Plasmid

#203838

Purpose

Flp-dependent Cre and mScarlet-KASH expression

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

Cre

Use

AAV

Tags

mScarlet-KASH-2A

Expression

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS/XBB.1.16-RBD

Plasmid

#213070

Purpose

Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.XBB.1.16 variant

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

pCAGGS/XBB.1.16-RBD (S )

Use

Tags

6 His Tag

Expression

Mammalian

Mutation

G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

Promoter

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_BRAF_p.D594H

Plasmid

#82816

Purpose

Gateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.

Depositor

Matthew Meyerson, Jesse Boehm, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

BRAF (BRAF Human)

Use

Gateway entry vector

Tags

Expression

Mutation

D594H

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1(nsp8-nsp7)(nsp12)

Plasmid

#165451

Purpose

Expresses the RNA Dependent RNA polymerase complex of SARS-CoV-2 in Escherichia Coli

Depositor

Marc Delarue

Article

Madru et al PLoS One. 2021 Apr 29;16(4):e0250610. do

Inserts

nsp12
nsp8
nsp7

Use

Tags

14Histidine-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam p53 R175H

Plasmid

#16436

Depositor

Bert Vogelstein

Article

Baker et al Science. 1990 Aug 24. 249(4971):912-5.

Insert

p53 R175H (TP53 Human)

Use

Tags

Expression

Mammalian

Mutation

R175H

Promoter

CMV

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-PRDX5WT-VA

Plasmid

#98688

Purpose

Lentiviral expression of human PRDX5-WT in mammalian cells

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

PRDX5 (PRDX5 Human)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-FLAG

Plasmid

#60360

Purpose

Plasmid for cloning and expression of FLAG-GFP tagged proteins (C-terminal tag). Confers resistance to G418.

Depositor

Patrick Calsou, Sébastien Britton

Article

Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Tags

EGFP and FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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Integrin alpha5-AHCys

Plasmid

#27313

Depositor

Timothy Springer

Article

Takagi et al Nat Struct Biol. 2001 May . 8(5):412-6.

Insert

integrin alpha 5 (ITGA5 Human)

Use

Tags

Expression

Mammalian

Mutation

integrin alpha5 (1-954) + AHCys

Promoter

Available since

March 8, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3-Venus-Venus-FLARE-Cameleon

Plasmid

#123351

Purpose

Yellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Venus-Venus-FLARE-Cameleon

Use

Tags

6xHIS, T7 tag (gene 10 leader), Venus, and Xpress…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV_BiPVe4_GCaMP6f

Plasmid

#213939

Purpose

AAV construct expressing GCaMP6f driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_GCaMP6f

Depositor

Gordon Fishell

Article

Furlanis et al bioRxiv 2024.07.17.603924

Insert

GCaMP6f

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBbE1k-DDC

Plasmid

#71723

Purpose

dopa decarboxylase from pig

Depositor

Taek Soon Lee

Article

Satoh et al Metab Eng. 2012 Nov;14(6):603-10. doi: 1

Insert

dopa decarboxylase

Use

Tags

Expression

Mutation

Promoter

pTrc

Available since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pGEM4Z-Fluc

Plasmid

#183474

Purpose

This plasmid can be used to prepare Fluc mRNA by In Vitro Transcription

Depositor

Chantal Pichon

Article

Perche et al Mol Ther Nucleic Acids. 2019 Sep 6;17:76

Insert

Firefly luciferase

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-mNeon

Plasmid

#174092

Purpose

CRISPaint gene tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

mNeonGreen

Use

Gene tagging

Tags

Expression

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-cytoEKAR4

Plasmid

#174438

Purpose

Cytosol-targeted EKAR4.

Depositor

Jin Zhang

Article

Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

Insert

cytoEKAR4

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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T7 promoter + terminators reporter plasmid

Plasmid

#156456

Purpose

A mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter and a terminator array.

Depositor

Matthew D. Shoulders

Article

Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

Inserts

NeoR/KanR
TcR

Use

Tags

Expression

Bacterial

Mutation

ATG start codon mutated to ACG

Promoter

Em7 and tet

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pSCrhaB2

Plasmid

#113634

Purpose

An expression vector containing a rhamnose-inducible promoter to regulate gene expression in Burkholderia cenocepacia

Depositor

Miguel Valvano

Article

Cardona et al Plasmid. 2005 Nov;54(3):219-28. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

PrhaB

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SARS-CoV-2-HiBiT-N

Plasmid

#215815

Purpose

Expresses N protein with HiBiT tag in mammalian cells

Depositor

Thomas Gallagher

Article

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Insert

SARS-CoV-2 HiBiT Nucleocapsid (N )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-hGFAP-NEUROD1-T2A-mCherry

Plasmid

#178582

Purpose

Expression of NEUROD1 and mCherry under the human GFAP promoter

Depositor

Chun-Li Zhang

Article

Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

Insert

NEUROD1 (NEUROD1 Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6d-IVT

Plasmid

#215836

Purpose

Template for in vitro transcription of CBE6d (with SpCas9)

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6d-SpCas9-2xUGI

Use

In vitro transcription template

Tags

Expression

Mutation

Promoter

Available since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-Tfe3-pgk-hph

Plasmid

#48755

Purpose

PiggyBac backbone, CAG promoter driving murine Tfe3, Hygromycin selection

Depositor

Austin Smith

Article

Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

Insert

Tfe3 (Tcfe3 Mouse)

Use

Piggybac

Tags

Expression

Mutation

Promoter

CAG

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only
pLVX-EF1alpha-SARS-CoV-2-nsp8-2xStrep-IRES-Puro

Plasmid

#141374

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp8 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-MMLVgag-3xNES-ABE8e

Plasmid

#181751

Purpose

MMLV-Gag fused to 3xNES-ABE8e with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

Depositor

David Liu

Article

Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

Insert

MMLVgag-3xNES-ABE8e

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-N-FLAG-HA-TBK1

Plasmid

#131791

Purpose

Lentiviral vector expressing WT TBK1

Depositor

Alicia Pickrell

Article

Sarraf et al Cell Rep. 2019 Oct 1;29(1):225-235.e5. d

Insert

TBK1 WT (TBK1 Human)

Use

Lentiviral

Tags

N-FLAG-HA

Expression

Mammalian

Mutation

WT full length

Promoter

CMV

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-Puro-TK-NLS-mTurq2-PCNA

Plasmid

#118617

Purpose

Lentiviral expression of mTurq2 tagged PCNA

Depositor

Jean Cook

Article

Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

Insert

TK-NLS-mTurq2-PCNA (PCNA Human)

Use

Lentiviral

Tags

mTurq2

Expression

Mammalian

Mutation

Promoter

hPGK

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pDualIN-SMN2

Plasmid

#214430

Purpose

Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7

Depositor

Chaolin Zhang

Article

Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

Insert

SMN2 EXON 7 (SMN2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-FLAG-SF2

Plasmid

#99021

Purpose

Mammalian expression of SF2

Depositor

Honglin Chen

Article

Huang et al Nat Commun. 2017 Mar 21;8:14751. doi: 10

Insert

SF2 (SRSF1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pKH3-human RSK1

Plasmid

#13841

Depositor

John Blenis

Article

Richards et al Mol Cell Biol. 2001 Nov . 21(21):7470-80

Insert

RSK1 (RPS6KA1 Human)

Use

Tags

3x HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pMTB

Plasmid

#112225

Purpose

Expression of membrane-tagged super folder GFP from zebrafish beta-actin promoter.

Depositor

Sean Megason

Article

Wagner et al Science. 2018 Apr 26. pii: science.aar43

Insert

Super-folder GFP

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

beta actin 2 (zebrafish)

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pJM220 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD)

Plasmid

#110559

Purpose

miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter

Depositor

Joanna Goldberg

Article

Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

Insert

rhaSR-PrhaBAD

Use

Tags

Expression

Bacterial

Mutation

Promoter

rhaSR-PrhaBAD

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-HA-SAMTOR (D190A)

Plasmid

#100515

Purpose

Expresses HA tagged SAMTOR D190A mutant in mammalian cells

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

SAMTOR (BMT2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

D190A

Promoter

Available since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Plenti-CMV-MCS-RFP-SV-puro

Plasmid

#109377

Purpose

This plasmid expresses dsRed2 and puromycin resistance

Depositor

Behzad Gerami-Naini, Jonathan Garlick

Type

Empty backbone

Use

Lentiviral

Tags

dsRed2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pPD49_83

Plasmid

#1448

Depositor

Andrew Fire

Type

Empty backbone

Use

Tags

hsp16-25', hsp16-415', IVSA, rf2128

Expression

Worm

Mutation

Promoter

Available since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe bleo human PPAR gamma 2

Plasmid

#11439

Depositor

Ronald Kahn

Insert

PPAR gamma 2 (PPARG Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH47751

Plasmid

#48002

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pSLIK-IDH1-R132H-FLAG

Plasmid

#66803

Purpose

Tet-inducible expression of mutant IDH1 in mammalian cells, 3rd generation lentiviral vector

Depositor

Christian Metallo

Article

Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

Insert

IDH1 (IDH1 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

R132H

Promoter

CMV

Available since

Feb. 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pUbi-MAD7

Plasmid

#170133

Purpose

For plant prime editing in wheat plants or monocotyledons protoplasts

Depositor

Caixia Gao

Article

Lin et al J Genet Genomics. 2021 May 3. pii: S1673

Insert

MAD7(ErCas12a)

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

maize Ubiquitin-1

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
tetO-FUW-eGFP

Plasmid

#73083

Purpose

Lentiviral transfer vector for tet-inducible expression of EGFP

Depositor

Andrew Putnam

Article

Kong et al Sci Rep. 2013 Dec 11;3:3474. doi: 10.103

Insert

Enhanced Green Fluorescent Protein

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-hsRPA32 (MSW#497)

Plasmid

#208068

Purpose

Expression of GFP-tagged hsRPA2 in human cells

Depositor

Marc Wold

Article

Haring et al J Biol Chem. 2008 Jul 4;283(27):19095-11

Insert

RPA2 (RPA2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

T88I- please see depositor comments

Promoter

Available since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV MP-135 mRFP

Plasmid

#206927

Purpose

The micro promoter MP-135 is an AAV specific strong promoter for gene expression in mammalian cells. The small size of the promoter (135 bp) allows delivery of large genes in AAV.

Depositor

Markus Grompe

Article

Chai et al Mol Ther Methods Clin Dev. 2023 May 17;2

Insert

mRFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

MP-135

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB2909(XII-5 MarkerFree)

Plasmid

#73281

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-5 (Chr XII: 839226..840357)

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge

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