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164,127 results

Showing: 9081 - 9120 of 164127 results

  1. psd44-GqQL

    Plasmid
    #46826
    Purpose
    2nd generation lentiviral vector for expression of mutant G protein alpha q in mammalian cells
    Depositor
    Agnese Mariotti
    Insert
    g protein alpha q (GNAQ Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Q209L (The mutation reduces GTPase activity resul…
    Promoter
    PGK promoter
    Available Since
    July 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    46826-plasmid-map-sequence-id-246440

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  2. hfCas12Max

    Plasmid
    #195336
    Purpose
    vector for encoding a human codon-optimized hfCas12Max driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Insert
    human codon-optimized hfCas12Max
    Use
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    N243R, E336R, D892R
    Promoter
    CBh, CMV, hU6
    Available Since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    195336-plasmid-map-sequence-id-389250

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  9. pFRT-TODestGFP_RNAseH1

    Plasmid
    #65784
    Purpose
    expresses GFP-tagged RNAseH1 in mammalian cells
    Depositor
    Thomas Tuschl
    Insert
    RNaseH1 (RNASEH1 Zebrafish, Human)
    Use
    Tags
    GFP and V5
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, 2x Tet
    Available Since
    June 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    65784-plasmid-map-sequence-id-111845

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  15. pRDA_816

    Plasmid
    #201157
    Purpose
    EnAsCas12a with a deactivated DNAse domain (D908A), N' 5x tandem ALFA-tags and C' VP64 and NLS for CRISPRa
    Depositor
    John Doench
    Article
    Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:
    Insert
    5x-ALFA tag-dEnAsCas12a-VP64
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    D908A
    Promoter
    EFS
    Available Since
    June 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    201157-plasmid-map-sequence-id-396590

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  18. EBFP2-N1

    Plasmid
    #54595
    Purpose
    Localization: N1 Cloning Vector, Excitation: 383, Emission: 448
    Depositor
    Michael Davidson
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    EBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54595-plasmid-map-sequence-id-364695

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  27. pJM656

    Plasmid
    #53086
    Purpose
    A non-Phosphatidylserine binding mutant form of Lactadherin(sGFP::LactC1C2mut) to be used as a control
    Depositor
    Ding Xue
    Article
    Mapes et al Curr Biol. 2012 Jul 24;22(14):1267-75. d
    Insert
    Lactaherin (Mfge8 Mouse)
    Use
    Tags
    GFP (w/ introns) and signal sequence
    Expression
    Worm
    Mutation
    deleted native signal sequence, deleted C-termina…
    Promoter
    hsp-16.41
    Available Since
    June 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    53086-plasmid-map-sequence-id-82567

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  30. pJRH-1346 U6-B2M sgRNA Gag-pol v2

    Plasmid
    #201917
    Purpose
    Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103
    Inserts
    Gag-pol
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and Human U6
    Available Since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    201917-plasmid-map-sequence-id-403313

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  33. PB-TET

    Plasmid
    #20909
    Purpose
    piggyBac empty vector with Gateway cassette, tet inducible promoter, and linked reporter (IRES-bgeo)
    Depositor
    Andras Nagy
    Article
    Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi
    Insert
    Gateway Destination Vector
    Use
    piggybac
    Tags
    IRES-bGeo
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    May 5, 2009
    Availability
    Academic Institutions and Nonprofits only
    20909-plasmid-map-sequence-id-174009

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  36. Cas12aUltra_5XNLS

    Plasmid
    #218775
    Purpose
    His-TEV-Cas12aUltra-BPSV40_cMyc_NP_5NLS
    Depositor
    Ross Wilson
    Article
    Sahu et al bioRxiv 2024.07.14.603391
    Insert
    5xNLS-BPSV40-NLP-cMyc-cMyc-BPSV40- AspCas12a
    Use
    CRISPR
    Tags
    6XHis (C terminal), HA, Nucleoplasmin, SV40, and …
    Expression
    Bacterial
    Mutation
    Nucleotides mutations were made to encode for arg…
    Promoter
    Available Since
    Aug. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    218775-plasmid-map-sequence-id-443448

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Showing: 9081 - 9120 of 164127 results