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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 12,578 results for: LIC

Showing: 11401 - 11450 of 12578 results

  3. pET21a - CPX

    Plasmid
    #89620
    Purpose
    [Edit] Expresses recombinant 6HIS tagged T. cruzi cytoplasmic tryparedoxin peroxidase protein in E. coli upon IPTG induction
    Depositor
    Judy Lieberman
    Article
    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1
    Insert
    Cytosolic Tryparedoxin peroxidase
    Use
    Tags
    6HIS and T7 tag (gene 10 leader)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    89620-plasmid-map-sequence-id-176689

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  7. Plas-CRISPR_reporter

    Plasmid
    #157658
    Purpose
    CRISPR reporter for mRNA quantification, integrative plasmid into NPR2 gene
    Depositor
    Frank Albert
    Article
    Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1
    Insert
    dCAS9-VP64, mCherry, KanMX
    Use
    Insert storage (replicative in e. coli)
    Tags
    Expression
    Mutation
    N28D in mCherry- please see depositor comments be…
    Promoter
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    157658-plasmid-map-sequence-id-309095

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  18. pCoofy62

    Plasmid
    #122014
    Purpose
    Mammalian vector for parallel SLIC cloning containing the VEGF signal sequence for secretion, N-terminal His6-Smt3Star (SumoStar*) and different C-tag options
    Depositor
    Sabine Suppmann
    Article
    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.
    Type
    Empty backbone
    Use
    Tags
    His6-Smt3Star and Multi3: depending on the LP2 pr…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    122014-plasmid-map-sequence-id-243584

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  25. pY128

    Plasmid
    #127713
    Purpose
    Level 1 yeast shuttle vector for a transcription unit with A - D scars. Clone in with BsaI. Has a high-copy yeast replicon and KanMX selection.
    Depositor
    Christopher Voigt
    Article
    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Dec. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    127713-plasmid-map-sequence-id-253011

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  28. pMEDS

    Plasmid
    #236568
    Purpose
    Introduces a multiple expression and dual selection (MEDS) system to use alongside CRISPR for applications such as gene editing.
    Depositor
    Shannon Moonah
    Article
    Sharma et al PLoS One. 2025 May 6;20(5):e0321881. doi
    Inserts
    CD/UPRT (negative selection)
    NPT II (positive selection)
    Use
    Tags
    3x Myc and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    236568-plasmid-map-sequence-id-477092

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  29. pDL0_212

    Plasmid
    #210778
    Purpose
    C2_Pyrearinus termitilluminans click beetle luciferase ('Eluc' version - human-codon optimized)
    Depositor
    Dmitri Nusinow
    Article
    Lee et al bioRxiv 2025.06.20.660755
    Insert
    Pyrearinus termitilluminans beetle luciferase ('Eluc' version - human-codon optimized)
    Use
    Luciferase and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    210778-plasmid-map-sequence-id-421287

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  30. ADEPT-pCas9

    Plasmid
    #238032
    Purpose
    Encodes Cas9 (ssrA degradation-tagged) under a tetracycline promoter (pTet) on a low-copy replication origin (SC101) as part of the ADEPT system.
    Depositor
    Lingchong You
    Article
    Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/
    Insert
    spCas9
    Use
    Synthetic Biology
    Tags
    ssrA
    Expression
    Mutation
    Promoter
    tet
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    238032-plasmid-map-sequence-id-475985

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  32. pJJB1559

    Plasmid
    #218590
    Purpose
    Express peroxisomal Idi1p, Erg20(F96W,N127W)p, and GESp to convert IPP to geraniol with cytosolic Erg20 homodimer
    Depositor
    John Dueber
    Article
    Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038
    Insert
    GES
    Use
    Tags
    ePTS1
    Expression
    Yeast
    Mutation
    Erg20(F96W, N127W), Erg20-Erg20 synthetic homodim…
    Promoter
    Available since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    218590-plasmid-map-sequence-id-432609

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  45. Cav-B

    Plasmid
    #209879
    Purpose
    Dimerization dependent fluorescent protein B anchored to cytosolic face of the caveolae with mouse CAVIN1
    Depositor
    Sarah Cohen
    Article
    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25
    Insert
    ddGFP B
    Use
    Tags
    CAVIN1
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    209879-plasmid-map-sequence-id-415404

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  47. pXC3cIfm

    Plasmid
    #193750
    Purpose
    Expresses frame-shifted CI under the control of PLUX promoter, p15A origin of replication, Chloramphenicol selection
    Depositor
    Sangram Bagh
    Article
    Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.
    Insert
    Frame-shifted CI
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLUX
    Available since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    193750-plasmid-map-sequence-id-390463

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  48. pLT237

    Plasmid
    #174300
    Purpose
    Replicating plasmid with homology region for introducing PolCC669Y mutation. Confers thiamphenicol resistance.
    Depositor
    Lee Lynd
    Article
    Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1
    Insert
    PolC homology arm
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changed Cysteine 669 to Tyrosine
    Promoter
    Available since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    174300-plasmid-map-sequence-id-392933

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  50. pPINA7A2mTFP1(F)

    Plasmid
    #193774
    Purpose
    Expresses mTFP1 under the control of PINA7 synthetic promoter, ColE1 origin of replication, Ampicillin selection
    Depositor
    Sangram Bagh
    Article
    Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.
    Insert
    mTFP1
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PINA7 synthetic promoter carrying binding sites f…
    Available since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    193774-plasmid-map-sequence-id-389814

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Showing: 11401 - 11450 of 12578 results