8,806 results

1. pORTMAGE-Ec1

   Plasmid

   #138474

   Purpose

   Enables high-efficiency recombineering in E. coli

   Depositor

   George Church

   Article

   Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

   Inserts

   CspRecT
   mutL E32K
   

   Expression

   Bacterial

   Mutation

   E32K

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-2xFLAG-SREBP-1c

   Plasmid

   #26802

   Depositor

   Timothy Osborne

   Article

   Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

   Insert

   SREBP-1c (SREBF1 Human)
   

   Tags

   2XFLAG

   Expression

   Mammalian

   Available Since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLJM1-FLAG-GFP-Tmem192

   Plasmid

   #134630

   Purpose

   lysosomal marker

   Depositor

   Roberto Zoncu

   Article

   Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

   Insert

   TMEM192 (TMEM192 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and GFP

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-GfaABC1D-MCS--4x6T-WPRE

   Plasmid

   #196417

   Purpose

   Empty vector for astrocyte-selective expression in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

   Depositor

   Stanley Thomas Carmichael

   Article

   Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

   Type

   Empty backbone

   Use

   AAV; Astrocyte-selective

   Expression

   Mammalian

   Promoter

   GfaABC1D

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTALdNC-AAVS1_T1

   Plasmid

   #80495

   Purpose

   AAVS1 TALEN expression vector (Left)

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Insert

   AAVS1 TALEN (Left)
   

   Use

   Gateway entry

   Promoter

   none

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTALdNC-AAVS1_T2

   Plasmid

   #80496

   Purpose

   AAVS1 TALEN expression vector (Right)

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Insert

   AAVS1 TALEN (Right)
   

   Use

   Gateway entry

   Promoter

   none

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV/SB11

   Plasmid

   #26552

   Depositor

   Perry Hackett

   Article

   Ivics et al Cell. 1997 Nov 14. 91(4):501-10.

   Insert

   Sleeping Beauty
   

   Expression

   Bacterial

   Mutation

   Methionine 243 to Glutamine Threonine 136 to Arg…

   Available Since

   Oct. 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. phH1-gRNA

   Plasmid

   #53186

   Purpose

   Expresses the S. pyogenes sgRNA from the H1 promoter

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   human H1

   Available Since

   June 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCSC-NGN2-IRES-GFP-T2A-Sox11

   Plasmid

   #90214

   Purpose

   To convert human skin fibroblasts into induced motor neurons (hiMN) in combination with ISL1, LHX3, FGF2 and two small molecules, forskolin and dorsomorphin.

   Depositor

   Chun-Li Zhang

   Article

   Liu et al Cell Rep. 2016 Jan 5;14(1):115-28. doi:

   Insert

   NGN2-IRES-GFP-T2A-Sox11 (NEUROG2, Sox11 Human, Mouse)
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE_puro

    Plasmid

    #118692

    Purpose

    Empty vector to express overexpression cDNA

    Depositor

    Christopher Vakoc

    Article

    Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

    Type

    Empty backbone

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmU6-gRNA

    Plasmid

    #53187

    Purpose

    Expresses the S. pyogenes sgRNA from the mouse U6 promoter

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    mouse U6

    Available Since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-sfGFP

    Plasmid

    #85492

    Purpose

    pET28a expressing codon optimized superfolder GFP with C-terminal His6 tag

    Depositor

    Ryan Mehl

    Article

    Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

    Insert

    superfolder GFP
    

    Tags

    6x His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV_BE4max_P2A_GFP

    Plasmid

    #112099

    Purpose

    C:G-to-T:A base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

    Insert

    BE4max_P2A_GFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. ph7SK-gRNA

    Plasmid

    #53189

    Purpose

    Expresses the S. pyogenes sgRNA from the 7SK promoter

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    human 7SK

    Available Since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFREE

    Plasmid

    #92050

    Purpose

    Allows inducible expression of gRNAs and Cas9 for plasmid curing.

    Depositor

    Morten Norholm

    Article

    Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

    Inserts

    Cas9
    gRNA array
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    pRhamBAD and ptet

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. lenti MS2-P65-HSF1_Hygro

    Plasmid

    #61426

    Purpose

    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Insert

    MS2-P65-HSF1_2A_Hygro (HSF1 Synthetic, Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    N55K in MS2

    Promoter

    EF1A

    Available Since

    Dec. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. M50 Super 8x TOPFlash

    Plasmid

    #12456

    Purpose

    Beta-catenin reporter. TCF/LEF sites upstream of a luciferase reporter.

    Depositor

    Randall Moon

    Article

    Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

    Insert

    TCF/LEF binding sites (Ctnnb1 )
    

    Use

    Luciferase

    Available Since

    March 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDY1173_ADAR1p150

    Plasmid

    #193192

    Purpose

    Express full length ADAR1p150

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

    Insert

    ADAR1p150 (ADAR Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CaMKIIa-hM3D(Gq)-mCherry

    Plasmid

    #50476

    Purpose

    Gq-coupled hM3D DREADD fused with mCherry under the control of CaMKIIa promoter

    Depositor

    Bryan Roth

    Has Service

    AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    hM3D(Gq)-mCherry (CHRM3 Human)
    

    Use

    AAV

    Tags

    mCherry

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    CaMKIIa

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro

    Plasmid

    #71236

    Purpose

    Express sgRNA and dCas9-KRAB from lentiviral vector

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

    Inserts

    humanized dCas9-KRAB T2A Puro
    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Mutation

    D10A and H840A

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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