8,723 results

1. pLX304 CMV FKBP-V5-sTurboID (N)

   Plasmid

   #153002

   Purpose

   Expresses low-affinity split-TurboID N-terminal fragment in mammalian cells

   Depositor

   Alice Ting

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

   Insert

   sTurboID (N) (L73)
   

   Use

   Lentiviral

   Tags

   FKBP and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. MP6

   Plasmid

   #69669

   Purpose

   Untargetted Bacterial Mutagenesis

   Depositor

   David Liu

   Article

   Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

   Insert

   dnaQ926 dam seqA emrR ugi CDA1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   E. coli PBAD

   Available since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. H2B-GFP

   Plasmid

   #11680

   Depositor

   Geoff Wahl

   Article

   Kanda et al Curr Biol. 1998 Mar 26. 8(7):377-85.

   Insert

   Human histone H2B (H2BC11 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 31, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLX304 CMV HA-HaloTag-FRB-sTurboID (C)

   Plasmid

   #153003

   Purpose

   Expresses low-affinity split-TurboID C-terminal fragment in mammalian cells

   Depositor

   Alice Ting

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

   Insert

   sTurboID (C) (G74)
   

   Use

   Lentiviral

   Tags

   FRB, HA, and HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3 APEX2-NES

   Plasmid

   #49386

   Purpose

   Expresses APEX2 in the cytosol of mammalian cells

   Depositor

   Alice Ting

   Article

   Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

   Insert

   Flag - APEX2 - NES
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   K14D, W41F, E112K, A134P on soybean APX; improved…

   Promoter

   CMV

   Available since

   Nov. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET His6 Thioredoxin TEV LIC cloning vector (2T-T)

   Plasmid

   #29712

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   His6-Thioredoxin-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pON.mCherry

   Plasmid

   #84821

   Purpose

   Constitutive Expression plasmid with mCherry fluorescent protein

   Depositor

   Howard Shuman

   Article

   Gebhardt et al PLoS One. 2017 Mar 3;12(3):e0173116. doi

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tac Promoter

   Available since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDY0423 Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)

   Plasmid

   #179116

   Purpose

   Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   Cargo EGFP with AttP Bxb1 site for in-frame fusion
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET His6 LIC cloning vector (2Bc-T)

   Plasmid

   #37236

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDY0663 ACTB atgRNA with v2 scaffold

    Plasmid

    #179108

    Purpose

    ACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA with v2 scaffold

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    ACTB N-term PBS 13 RT 29 AttB 46 atgRNA with v2 scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET His6 GST TEV LIC cloning vector (2G-T)

    Plasmid

    #29707

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, GST, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET His6 TEV LIC cloning vector (2B-T)

    Plasmid

    #29666

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. LV_EF1a_SV40_Large_T_Antigen-P2A-Hygro_Barcode

    Plasmid

    #170255

    Purpose

    Barcoded lentiviral vector to express SV40 Large T Antigen in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    SV40 Large T Antigen
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-PE6c

    Plasmid

    #207853

    Purpose

    Mammalian expression of PE6c prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6c
    

    Use

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL3 Basic Vector

    Plasmid

    #212936

    Purpose

    Firefly luciferase vector for investigating regions controlling transcription

    Depositor

    Debrya Groskreutz

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Jan. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. 3XMEF2-luc

    Plasmid

    #32967

    Purpose

    Reporter gene for MEF2 activity with 3 MEF2 sites upstream of a c-fos minimal promoter

    Depositor

    Ron Prywes

    Insert

    MEF2 binding sites (MEF2A Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    MEF2

    Available since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. EGFP

    Plasmid

    #176015

    Purpose

    Mammalian expression of EGFP. Parton lab clone DFS

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-PEmax-P2A-GFP

    Plasmid

    #180020

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-EGFP marker

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PEmax-P2A-EGFP
    

    Use

    Tags

    P2A-EGFP, SV40 bpNLS, and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA3.1(+)-CMV-bArrestin2-TEV

    Plasmid

    #107245

    Purpose

    Vector for preparation of stable cell lines expressing Barrestin2-TEV fusion protein in lieu of the HTLA cell line.

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    ARRB2
    

    Use

    Tags

    TEV(NIA), tobacco etch virus NIA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. FLT3-ITD-MSCV-IRES-GFP

    Plasmid

    #184779

    Purpose

    Expresses FLT3-ITD (internal tandem duplication) constitutively active mutant in mammalian cells

    Depositor

    Timothy Ley

    Insert

    FLT3 (FLT3 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Activating internal tandem duplication (ITD) adde…

    Promoter

    MSCV

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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