We narrowed to 4,070 results for: p2a

1. pFH2.11_Gag-MCP_ZF2-P2A-eGFP

   Plasmid

   #205526

   Purpose

   Express HIV Gag-MCP_ZF2-P2A-eGFP

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   Gag-MCP_ZF2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMK322 (TOP2A-mAC (Hygro))

   Plasmid

   #140653

   Purpose

   TOP2A tagging with mAID-Clover

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   TOP2A (TOP2A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. SOX2-P2A-tagBFP-HDR

   Plasmid

   #163751

   Purpose

   HDR knock-in template for tagging the human endogenous SOX2 gene with a tagBFP fluorescent marker linked by a self-cleaving P2A peptide.

   Depositor

   Jennifer Mitchell

   Article

   Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

   Insert

   SOX2 (SOX2 Human)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   P2A-tagBFP

   Expression

   Mutation

   Does not contain start codon to avoid random inte…

   Promoter

   Available since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Fuw-AcrIIA4-P2A-GFP

   Plasmid

   #108247

   Purpose

   Lentiviral vector to express AcrIIA4-P2A-GFP

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

   Insert

   AcrIIA4-NLS-HA (NEWENTRY Synthetic)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Ubc

   Available since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX307 P2A GFP PRDM14

   Plasmid

   #193676

   Purpose

   Constitutive lentiviral expression of PRDM14

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   PRDM14 (PRDM14 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSMVP-Cas9FL-P2A-turboGFP

   Plasmid

   #80941

   Purpose

   Plasmid that expresses Cas9FL in mammalian cells; co-expresses turboGFP.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9FL
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTarget TMEM97-P2A-mCardinal

   Plasmid

   #157747

   Purpose

   Express in mammalian cells TMEM97 and mCardianl to follow transfection efficiency using flow cytometry. mCardinal is expressed following a P2A skip peptide so it is not connected to TMEM97.

   Depositor

   Andrew Kruse

   Article

   Alon et al Proc Natl Acad Sci U S A. 2017 Jul 3;114

   Insert

   TMEM97 (TMEM97 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pCMV-PEmax-P2A-hP53DD

   Plasmid

   #214084

   Purpose

   Mammalian expression of SpCas9 PEmax prime editor with P2A-hP53DD

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Insert

   PEmax-P2A-hP53DD (TP53 Synthetic, Human, SpCas9 is from Streptococcus pyogenes; MMLV_RT is from the Moloney murine leukemia virus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLC-CUL4A WT-P2A-Puro

   Plasmid

   #124304

   Purpose

   Lentiviral vector for expression of wild-type CUL4A-P2A-Puro casette from a CMV promoter

   Depositor

   Eva Gottwein

   Article

   Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

   Insert

   CUL4A (CUL4A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pdx1-mTQ2-P2A-FlpO-PA

    Plasmid

    #67279

    Purpose

    Pancreas specific expression of the FlpO recombinase controlled by the pdx1 promoter . FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context

    Depositor

    Jannik Elverløv-Jakobsen

    Inserts

    mTQ2=mturquoise2 blue fluorescent gene
    FlpO recombinase
    

    Use

    Tags

    linked to FlpO through an P2A ribosomal skipping …

    Expression

    Mammalian

    Mutation

    Promoter

    pdx

    Available since

    July 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCR8-Magneto2.0-p2A-mCherry

    Plasmid

    #74334

    Purpose

    For gateway cloning Magneto2.0-p2A-mCherry into destination vector

    Depositor

    Ali Guler

    Article

    Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

    Insert

    Magneto2.0 (FTH1, FTL, Trpv4 Human, Rat)
    

    Use

    Gateway vector

    Tags

    Flag and p2A-mCherry

    Expression

    Mutation

    TRPV4: delta 760–871

    Promoter

    none

    Available since

    May 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR_TRE3GS-->GssGFP-P2A-NLSIFP

    Plasmid

    #162235

    Purpose

    Lentiviral vector for the expression of TRE3GS-->GssGFP-P2A-NLSIFP in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2020 Oct 16;370(6514):327-331.

    Insert

    GFP - IFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGTag-P2A-QFGal4-SV40pA

    Plasmid

    #155127

    Purpose

    Donor for precise CRISPR directed genomic integration of QFGal4 using the GeneWeld method

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    P2A-QFGal4
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pALPS tat-P2A-rev

    Plasmid

    #101331

    Purpose

    Lentivector expressing codon optimized NL4-3 tat and rev linked by P2A peptide coding sequence (GSGATNFSLLKQAGDVEENPGP)

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3 tat and rev
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. p3E-APEX2-P2A-mKate2

    Plasmid

    #67671

    Purpose

    Multisite gateway entry clone for adding C-terminal fusions of APEX2-P2A-mKate2

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    APEX2-P2A-mKate2
    

    Use

    Gateway multisite 3' entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV2-GCaMP6f-P2A-Vmn1r237

    Plasmid

    #133417

    Purpose

    Fluorescent calcium reporter and mouse vomeronasal 1 receptor 237

    Depositor

    Tim Holy

    Article

    Lee et al Science. 2019 Dec 13;366(6471):1384-1389

    Insert

    GCaMP6f-P2A-vmn1r237 (Vmn1r237 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCF287_EFS-TiCas9-P2A-Puro

    Plasmid

    #211646

    Purpose

    EFS-TiCas9-P2A-Puro. Tamoxifen-inducible SpyCas9.

    Depositor

    Christof Fellmann

    Article

    Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

    Insert

    Tamoxifen-inducible SpyCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV2-GCaMP6f-P2A-Vmn1r78

    Plasmid

    #133413

    Purpose

    Fluorescent calcium reporter and mouse vomeronasal 1 receptor 78

    Depositor

    Tim Holy

    Article

    Lee et al Science. 2019 Dec 13;366(6471):1384-1389

    Insert

    GCaMP6f-P2A-vmn1r78 (Vmn1r78 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-BE4RA-P2A-Puro

    Plasmid

    #112672

    Purpose

    Lentivirus for constitutive expression of BE4RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE4RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    NLS sequence at the N-terminus and D10A

    Promoter

    EF1s

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pME-EGFP-P2A-APEX2-NS

    Plasmid

    #108895

    Purpose

    Multisite gateway vector for 5' tagging with APEX2 (includes cytoplasmic GFP reporter)

    Depositor

    Rob Parton

    Article

    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

    Insert

    EGFP-P2A-APEX2
    

    Use

    Multisite gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV2-GCaMP6f-P2A-Vmn1r86

    Plasmid

    #133415

    Purpose

    Fluorescent calcium reporter and mouse vomeronasal 1 receptor 86

    Depositor

    Tim Holy

    Article

    Lee et al Science. 2019 Dec 13;366(6471):1384-1389

    Insert

    GCaMP6f-P2A-vmn1r86 (Vmn1r86 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV2-GCaMP5g-P2A-Vmn1r58

    Plasmid

    #133418

    Purpose

    Fluorescent calcium reporter and mouse vomeronasal 1 receptor 58

    Depositor

    Tim Holy

    Article

    Lee et al Science. 2019 Dec 13;366(6471):1384-1389

    Insert

    GCaMP5g-P2A-PAmCherry-Vmn1r58 (Vmn1r58 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSMVP-Cas9C-P2A-turboGFP

    Plasmid

    #80935

    Purpose

    Plasmid that expresses Cas9C in mammalian cells; co-expresses turboGFP.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9C
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMK376 (Hygro-P2A-mClover)

    Plasmid

    #121187

    Purpose

    N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    Hygro-P2A-mClover
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pKLV2-U6gRNA5(Empty)-PGKGFP2ABFP-W

    Plasmid

    #67983

    Purpose

    Cas9 activity reporter (control) with GFP and BFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKGFP2ABFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLenti-xBE3-P2A-Puro

    Plasmid

    #110870

    Purpose

    Lentiviral vector for constitutive expression of xCas9(3.7)-BE3 in mammalian cells

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    xCas9(3.7)-BE3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA5-FRT/T0-Flag-CIP2A

    Plasmid

    #222623

    Purpose

    mammalian expression vector of Flag-tagged CIP2A

    Depositor

    Daniel Durocher

    Article

    Adam et al Nat Cancer. 2021 Dec;2(12):1357-1371. do

    Insert

    CIP2A (CIP2A Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pFH2.16_MiniGagZip-MCP_Zip-P2A-eGFP

    Plasmid

    #205531

    Purpose

    Express HIV MiniGagZip-MCP_Zip-P2A-eGFP

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    MiniGagZip-MCP_Zip
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMY-MCS-P2A-Ly6G

    Plasmid

    #163353

    Purpose

    Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated Ly6G surface marker

    Depositor

    Teunis Geijtenbeek, Louis Ates

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMY-eGFP-P2A-PuroR-HA

    Plasmid

    #163343

    Purpose

    Retroviral vector to express cytosolic GFP and an HA-tagged puromycin selection marker

    Depositor

    Teunis Geijtenbeek, Louis Ates

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Insert

    GFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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