We narrowed to 2,111 results for: D10

1. pXSE901B

   Plasmid

   #91713

   Purpose

   CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Bar resistance

   Depositor

   Qi-Jun Chen

   Article

   Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUHD Cdk2 WT HA

   Plasmid

   #27650

   Depositor

   Greg Enders

   Article

   Hu et al Mol Cell Biol. 2001 Apr . 21(8):2755-66.

   Insert

   Cdk2 (CDK2 Human)
   

   Use

   Tags

   HA (YDVPDYA)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

3. AAV_A2S-8

   Plasmid

   #188654

   Purpose

   AAV vector expressing SaAID-2S and gRNA

   Depositor

   Keiji Nishida

   Article

   Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

   Inserts

   AID2S--nSaCas9-UGI
   Sa-sgRNA
   

   Use

   AAV and CRISPR

   Tags

   UGI

   Expression

   Mammalian

   Mutation

   D10A for SaCas9

   Promoter

   Scp1 and U6

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGBW-m4095420

   Plasmid

   #149523

   Purpose

   Bacterial expression plasmid for SARS-CoV-2 nsp3

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   d1-745 d1063-1945

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pRS315e_pGal-dCas9-SH3

   Plasmid

   #79613

   Purpose

   Expresses dCas9-SH3 in yeast cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Use

   Tags

   SH3 domain

   Expression

   Yeast

   Mutation

   D10A and H840A for nuclease deficient Cas9

   Promoter

   pGal1

   Available since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. NAX_CAGGS_CAS9_m4_EYFP

   Plasmid

   #167866

   Purpose

   PiggyBac compatible plasmid expressing dCas9

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGBW-m4095421

   Plasmid

   #149524

   Purpose

   Bacterial expression plasmid for SARS-CoV-2 nsp3

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   d1-745 d1063-1945

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. BPK1160

   Plasmid

   #179298

   Purpose

   Expresses dCas9 fused to P65

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

   Insert

   dCas9-p65
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive Cas9)

   Promoter

   CAG

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJK413

   Plasmid

   #155372

   Purpose

   dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus] (sgRNAs flip of 412)

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas9 (bacteria)
   PA4-mVenus
   dCas9 sgRNA oscillator v1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUHD Cdk2 DN HA

    Plasmid

    #27651

    Depositor

    Greg Enders

    Article

    Hu et al Mol Cell Biol. 2001 Apr . 21(8):2755-66.

    Insert

    Cdk2 (CDK2 Human)
    

    Use

    Tags

    HA (YDVPDYA)

    Expression

    Mammalian

    Mutation

    D146N (Dominant Negative)

    Promoter

    Available since

    Feb. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pJK429

    Plasmid

    #155376

    Purpose

    PphlF_A4NT in dCRv1, 1x cascade + [dCas9 + PA4-mVenus]

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    PphlF_A4NT in dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. XE42 Rat frizzled-2-CS2+

    Plasmid

    #16814

    Depositor

    Randall Moon

    Article

    Yang-Snyder et al Curr Biol. 1996 Oct 1. 6(10):1302-6.

    Insert

    rat frizzled 2 (Fzd2 Rat)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 13, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pAG415Gal_Gcn4 solvvol

    Plasmid

    #232976

    Purpose

    Galactose iduced expression of Gcn4 solvvol in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 solvvol
    

    Use

    Tags

    TEV cleavage site

    Expression

    Yeast

    Mutation

    S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAG415Gal_Gcn4 solvvol W

    Plasmid

    #232977

    Purpose

    Galactose iduced expression of Gcn4 solvvol W+ in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 solvvol W+
    

    Use

    Tags

    3xHA and TEV cleavage site

    Expression

    Yeast

    Mutation

    S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CLIPf-KIF5A(1-573)

    Plasmid

    #219683

    Purpose

    Mammalian expression of CLIPf-KIF5A(1-573)

    Depositor

    Alison Twelvetrees

    Article

    Smith et al bioRxiv 2024.12.20.629623

    Insert

    KIF5A (Kif5a Mouse)
    

    Use

    Tags

    CLIPf

    Expression

    Mammalian

    Mutation

    1-573

    Promoter

    CMV & T7

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CLIPf-KIF5A

    Plasmid

    #200790

    Purpose

    Mammalian expression of CLIPf-KIF5A

    Depositor

    Alison Twelvetrees

    Article

    Smith et al bioRxiv 2024.12.20.629623

    Insert

    KIF5A (Kif5a Mouse)
    

    Use

    Tags

    CLIPf

    Expression

    Mammalian

    Mutation

    Promoter

    CMV & T7

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJMP8835

    Plasmid

    #220902

    Purpose

    MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (mscar targeting), and PG-dCas9-novo

    Depositor

    Jason Peters

    Article

    Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

    Insert

    dCas9-novo
    

    Use

    CRISPR

    Tags

    3xMyc

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJMP8921

    Plasmid

    #220903

    Purpose

    MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (BsaI site), and PC-dCas9-novo

    Depositor

    Jason Peters

    Article

    Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

    Insert

    dCas9-novo
    

    Use

    CRISPR

    Tags

    3xMyc

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJMP8923

    Plasmid

    #220904

    Purpose

    MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (BsaI site), and PG-dCas9-novo

    Depositor

    Jason Peters

    Article

    Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

    Insert

    dCas9-novo
    

    Use

    CRISPR

    Tags

    3xMyc

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJMP8932

    Plasmid

    #220906

    Purpose

    MobileCRiSPRi system encoded on a kanR Tn7 transposon; PD-sgRNA (BsaI site) and PG-dCas9-novo (for cloning new guides)

    Depositor

    Jason Peters

    Article

    Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

    Insert

    dCas9-novo
    

    Use

    CRISPR

    Tags

    3xMyc

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET3a-UBE2G2

    Plasmid

    #204209

    Purpose

    Bacterial expression of UBE2G2

    Depositor

    Allan Weissman

    Article

    Das et al Mol Cell. 2009 Jun 26. 34(6):674-85.

    Insert

    UBE2G2 (Ube2g2 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. NAX_CAGGS_CAS9_m4_mcherry

    Plasmid

    #167870

    Purpose

    PiggyBac compatible plasmid expressing dCas9

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

23. pFlare9A-CD45i4e5i5

    Plasmid

    #131834

    Purpose

    A reporter construct containing the Exon 5 of PTPRC (CD45), which contains a CTCF binding site.

    Depositor

    Mark Minden

    Article

    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

    Insert

    PTPRC (PTPRC Human)
    

    Use

    Reporter construct

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. BPK617

    Plasmid

    #179297

    Purpose

    Expresses dCas9 fused to VP64

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

    Insert

    dCas9-VP64
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive Cas9)

    Promoter

    CAG

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. NAX_CAGGS_CAS9_m4_iRFP713

    Plasmid

    #167868

    Purpose

    PiggyBac compatible plasmid expressing dCas9

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. NAX_CAGGS_CAS9_m4_Hygro

    Plasmid

    #167867

    Purpose

    PiggyBac compatible plasmid expressing dCas9

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. NAX_CAGGS_CAS9_m4_EBFP2

    Plasmid

    #167865

    Purpose

    PiggyBac compatible plasmid expressing dCas9

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p415:iCas9

    Plasmid

    #138266

    Purpose

    Expression of mTN3-GGS6-dCas9 (iCas9) in yeast.

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    mTN3-GGS6-dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Cas9-D10A, H840A

    Promoter

    GalL

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV-ABEmax(TadA E59A, TadA*R47F)

    Plasmid

    #125658

    Purpose

    pCMV-ABEmax(TadA E59A, TadA*R47F) in mammalian cells

    Depositor

    David Liu

    Article

    Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

    Insert

    ABEmax(TadA E59A TadA* D108F)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJK412

    Plasmid

    #155371

    Purpose

    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pJK430

    Plasmid

    #155377

    Purpose

    PphlF_A2NT in dCRv1, 2x cascade + [dCas9 + PA4-mVenus]

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    PphlF_A2NT in dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. YE1-BE4max

    Plasmid

    #138155

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

    Insert

    rAPOBEC1(YE1)-nSpCas9-UGI-UGI
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Within rAPOBEC1 W90Y + R126E; within Cas9 D10A

    Promoter

    CMV

    Available since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pJMP1356

    Plasmid

    #119276

    Purpose

    for cloning new sgRNAs

    Depositor

    Jason Peters, Oren Rosenberg, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pJMP1183

    Plasmid

    #119254

    Purpose

    rfp "test" strain

    Depositor

    Jason Peters, Oren Rosenberg, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pJMP1185

    Plasmid

    #119255

    Purpose

    rfp "test" strain

    Depositor

    Jason Peters, Oren Rosenberg, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. LAMP1-mGFP

    Plasmid

    #34831

    Depositor

    Esteban Dell'Angelica

    Article

    Falcon-Perez et al J Cell Sci. 2005 Nov 15;118(Pt 22):5243-

    Insert

    LAMP1 (LAMP1 Human)
    

    Use

    Tags

    GSTGSTGSTGA linker, Kozak, and mGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLAMP1-mCherry

    Plasmid

    #45147

    Depositor

    Amy Palmer

    Article

    Van Engelenburg et al Nat Methods. 2010 Apr;7(4):325-30. doi:

    Insert

    LAMP1-mCherry (LAMP1 Human, Synthetic)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCMV-SpRY-ABE8e

    Plasmid

    #185671

    Purpose

    Expresses SpRY-ABE8e in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-nSpRY
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    SpRY D10A

    Promoter

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pdCRISPomyces-2BD

    Plasmid

    #234429

    Purpose

    Plasmid for Streptomyces containing gene of modified dCas9-BD protein and sequence of sgRNA cloning template

    Depositor

    Min-Kyu Oh

    Article

    Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:

    Insert

    Modified dCas9(D10A, H840A) with polyaspartate linking
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Linked polyaspartate using glycine-serine linker

    Promoter

    Available since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pXPR_120

    Plasmid

    #96917

    Purpose

    for CRISPRa, lentiviral expression of dCas9-VPR 2A BlastR

    Depositor

    John Doench, David Root

    Article

    Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    VPR

    Expression

    Mammalian

    Mutation

    D10A, N863A

    Promoter

    EF1a

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pJMP1358

    Plasmid

    #119277

    Purpose

    for cloning new sgRNAs

    Depositor

    Jason Peters, Oren Rosenberg, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. human STIM1 YFP

    Plasmid

    #19754

    Depositor

    Anjana Rao

    Article

    Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Retroviral

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. human STIM1 CFP

    Plasmid

    #19755

    Depositor

    Anjana Rao

    Article

    Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Retroviral

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCMV-SpRY-HF1-ABE8e

    Plasmid

    #185670

    Purpose

    Expresses SpRY-HF1-ABE8e in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-nSpRY-HF1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    SpRY-HF1 D10A

    Promoter

    Available since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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45. pHAGE TRE dCas9-KRAB

    Plasmid

    #50917

    Purpose

    Tet-regulatable dCas9-KRAB 2nd generation lentiviral expression vector

    Depositor

    Rene Maehr, Scot Wolfe

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and KRAB

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive)

    Promoter

    TRE

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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46. pHAGE TRE dCas9-VP64

    Plasmid

    #50916

    Purpose

    Tet-regulatable dCas9-VP64 2nd generation lentiviral expression vector

    Depositor

    Rene Maehr, Scot Wolfe

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and VP64

    Expression

    Mammalian

    Mutation

    D10A, H840A nuclease-deficient

    Promoter

    TRE

    Available since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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47. pTagBFP-C1-FKBP-PIP5K1C-HD-EE

    Plasmid

    #220090

    Purpose

    Recruitable PIP5K to make PIP2

    Depositor

    Gerry Hammond

    Article

    Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

    Insert

    PIP5K1C (PIP5K1C Human)
    

    Use

    Tags

    TagBFP-FKBP1A(3-108)

    Expression

    Mammalian

    Mutation

    amino acids 1-635; D101R, R304D, R445E, K446E

    Promoter

    CMV

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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48. pcDNA3-ADAM10-HA

    Plasmid

    #65106

    Purpose

    Expression of HA tagged ADAM10 in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

    Insert

    ADAM10 (ADAM10 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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49. SpdCas9 VPR

    Plasmid

    #188509

    Purpose

    Expresses FLAG tagged SpdCas9 VPR

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A/H840A

    Promoter

    EF1a

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. SpdCas9 KRAB

    Plasmid

    #188499

    Purpose

    Expresses FLAG tagged SpdCas9 KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A/H840A

    Promoter

    EF1a

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge