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Plasmid#141649PurposeLentiviral vector for overexpressing the HOXC6 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHOXC6 (HOXC6 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_BRAF_p.G466V
Plasmid#81710PurposeGateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.DepositorInsertBRAF (BRAF Human)
UseGateway entry vectorTagsExpressionMutationG466VPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_BRAF_p.L597Q
Plasmid#81684PurposeGateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.DepositorInsertBRAF (BRAF Human)
UseGateway entry vectorTagsExpressionMutationL597QPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hnRNPU-V5
Plasmid#35974DepositorInsertheterogeneous nuclear ribonucleoprotein U (HNRNPU Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
Salsa6f
Plasmid#140188PurposeSalsa6f is a fluorescent ratiometric genetically encoded calcium indicator, (tdTomato linked to GCaMP6f by a V5 epitope tag)DepositorInsertSalsa6f
UseTagstdTomato-v5-GCaMP6fExpressionMammalianMutationPromoterCMV Immediate EarlyAvailable sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
BRR2 pRS313
Plasmid#111411PurposeBrr2 (endogenous promoter)DepositorInsertBRR2
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3. Flag-CSL R192H
Plasmid#167005PurposeMammalian expression of CSL R192HDepositorInsertCSL (RBPJ Human)
UseTagsFLAGExpressionMammalianMutationR192HPromoterCMVAvailable sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ExRai-AMPKAR(T/A)
Plasmid#192447PurposeNon-phosphorylatable negative-control mutant of ExRai-AMPKAR.DepositorInsertExRai-AMPKAR(T/A)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tagExpressionMammalianMutationThr 6 in AMPK substrate mutated to Ala.PromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
FKBP-V5-AP-nes_pLX304
Plasmid#120912PurposeLentiviral vector expressing FKBP-V5-AP-NES in mammalian cytosolDepositorInsertFKBP-V5-AP-NES
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGGAselect
Plasmid#195714PurposepGGAselect is a cloning vector for Golden Gate Assembly using BsaI, BsmBI, and BbsI Type IIS restriction enzymes.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDY0209 ACTB Nicking guide +48
Plasmid#179109PurposeACTB N-term Nicking guide 1 +48 guideDepositorInsertACTB N-term Nicking guide 1 +48 guide
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Actin-7
Plasmid#56421PurposeLocalization: Actin, Excitation: 488, Emission: 507DepositorInsertActin (ACTB Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 23, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTon1(acgfp1)
Plasmid#233648PurposeExpression vector of AcGFP1 under control of tetracycline responsive element (TRE). Also express Tet-on advenced transactivator (rtTA) under control of minimal CMV promoter.DepositorInserttarget sequence of guide RNA #tyr (3253-3275) 5'-GGCTCCATGTCTTCCGTCCAAGG-3'
UseAmphibian expression, tet-onTagsExpressionMutationPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTon1(il11)
Plasmid#233647PurposeExpression vector of X. laevis il11.L-P2A-AcGFP1 under control of tetracycline responsive element (TRE). Also express Tet-on advenced transactivator (rtTA) under control of minimal CMV promoter.DepositorInsertsinterleukin-11.L (350-913) (il11.L Frog)
target sequence of guide RNA #tyr (3905-3927) 5'-GGCTCCATGTCTTCCGTCCAAGG-3'
UseAmphibian expression, tet-onTagsExpressionMutationSome synonymous substitutions were introduced in…PromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
p324_DG
Plasmid#234690PurposeGolden gate domesticated pET28b with TEV-His6DepositorTypeEmpty backboneUseTagsTEV-His6ExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p370_DG
Plasmid#234693PurposeGolden gate domesticated pET28b with 3C-mNeongreen-His10DepositorTypeEmpty backboneUseTags3C-mNeongreen-His10ExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p277_DG
Plasmid#234695PurposeGolden gate domesticated pET28b for scarless cloning with His6-3CDepositorTypeEmpty backboneUseTagsHis6-3CExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p357_DG
Plasmid#234700PurposeGolden gate domesticated pET28b for scarless cloning with His10-GST-3CDepositorTypeEmpty backboneUseTagsHis10-GST-3CExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p368_DG
Plasmid#234691PurposeGolden gate domesticated pET28b with 3C-His6DepositorTypeEmpty backboneUseTags3C-His6ExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p349_DG
Plasmid#234679PurposeGolden gate domesticated pET28b with His10-GST-3CDepositorTypeEmpty backboneUseTagsHis10-GST-3CExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p351_DG
Plasmid#234681PurposeGolden gate domesticated pET28b with His10-mNeongreen-3CDepositorTypeEmpty backboneUseTagsHis10-mNeongreen-3CExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p364_DG
Plasmid#234686PurposeGolden gate domesticated pET28b with His10-bdNEDD8 and OpsinDepositorTypeEmpty backboneUseTagsHis10-bdNEDD8 and OpsinExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p369_DG
Plasmid#234692PurposeGolden gate domesticated pET28b with 3C-His10DepositorTypeEmpty backboneUseTags3C-His10ExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p344_DG
Plasmid#234674PurposeGolden gate domesticated pET28b with His6-3CDepositorTypeEmpty backboneUseTagsHis6-3CExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p367_DG
Plasmid#234689PurposeGolden gate domesticated pET28b with His10-bdNEDD8 and 2X Strep tagDepositorTypeEmpty backboneUseTags2X Strep tag and His10-bdNEDD8ExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p354_DG
Plasmid#234697PurposeGolden gate domesticated pET28b for scarless cloning with His10-TEVDepositorTypeEmpty backboneUseTagsHis10-TEVExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p358_DG
Plasmid#234701PurposeGolden gate domesticated pET28b for scarless cloning with His10-bdNEDD8DepositorTypeEmpty backboneUseTagsHis10-bdNEDD8ExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p359_DG
Plasmid#234702PurposeGolden gate domesticated pET28b for scarless cloning with His10-mNeongreen-3CDepositorTypeEmpty backboneUseTagsHis10-mNeongreen-3CExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p360_DG
Plasmid#234703PurposeGolden gate domesticated pET28b for scarless cloning with His10-mNeongreen-bdNEDD8DepositorTypeEmpty backboneUseTagsHis10-mNeongreen-bdNEDD8ExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p346_DG
Plasmid#234676PurposeGolden gate domesticated pET28b with His10-TEVDepositorTypeEmpty backboneUseTagsHis10-TEVExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p348_DG
Plasmid#234678PurposeGolden gate domesticated pET28b with His10-MBP-3CDepositorTypeEmpty backboneUseTagsHis10-MBP-3CExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p350_DG
Plasmid#234680PurposeGolden gate domesticated pET28b with His10-bdNEDD8DepositorTypeEmpty backboneUseTagsHis10-bdNEDD8ExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p356_DG
Plasmid#234699PurposeGolden gate domesticated pET28b for scarless cloning with His10-MBP-3CDepositorTypeEmpty backboneUseTagsHis10-MBP-3CExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p345_DG
Plasmid#234675PurposeGolden gate domesticated pET28b with His10-3CDepositorTypeEmpty backboneUseTagsHis10-3CExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p347_DG
Plasmid#234677PurposeGolden gate domesticated pET28b with His10-ThrombinDepositorTypeEmpty backboneUseTagsHis10-ThrombinExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p355_DG
Plasmid#234698PurposeGolden gate domesticated pET28b for scarless cloning with His10-ThrombinDepositorTypeEmpty backboneUseTagsHis10-ThrombinExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p352_DG
Plasmid#234682PurposeGolden gate domesticated pET28b with His10-mNeongreen-bdNEDD8DepositorTypeEmpty backboneUseTagsHis10-mNeongreen-bdNEDD8ExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p363_DG
Plasmid#234685PurposeGolden gate domesticated pET28b with His10-bdNEDD8 and SortaseDepositorTypeEmpty backboneUseTagsHis10-bdNEDD8 and SortaseExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p353_DG
Plasmid#234696PurposeGolden gate domesticated pET28b for scarless cloning with His10-3CDepositorTypeEmpty backboneUseTagsHis10-3CExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRecE564T
Plasmid#226825PurposeExpressing RecE*T controled by Ptrc promoterDepositorInsertrecE*T
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only