We narrowed to 22,191 results for: his

1. pMW-PahZ1 KT-1

   Plasmid

   #141134

   Purpose

   Expresses in BL-21 (DE3) cells to produce the poly(aspartic acid) hydrolase-1 from Sphingomonas Sp. KT-1 which selectively cleaves the beta linkages in thermally synthesized poly(aspartic acid).

   Depositor

   Mitch Weiland

   Article

   Bolay et al Journal of Chemical Education

   Insert

   PahZ1
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Not the full-length gene. PahZ1 fragment starts a…

   Promoter

   Available Since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLz33_CsoS2_NTD_GFP

   Plasmid

   #140861

   Purpose

   His-tagged CsoS2 N-terminal domain (NTD) fused to Superfolder GFP

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CsoS2 NTD fused to GFP
   

   Use

   Tags

   Hexahistidine tag and Superfolder GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. NdeI – SacII – SpeI(NheI) - ANXA2 (29-339)

   Plasmid

   #136545

   Purpose

   Expresses ANXA2 chimeras in bacterial cells

   Depositor

   Laszlo Nyitray

   Article

   Ecsedi et al Structure. 2020 Aug 4;28(8):943-953.e4.

   Type

   Empty backbone

   Use

   Tags

   ANXA2 (29-339) and His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pWDY1226

   Plasmid

   #138593

   Purpose

   Expresses NatL2-R156A in E.coli

   Depositor

   James Naismith

   Article

   Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

   Insert

   phenylacetate-coenzyme A ligase
   

   Use

   Tags

   6-His

   Expression

   Bacterial

   Mutation

   changed Arginine 156 to Alanine

   Promoter

   T7

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pWDY1222

   Plasmid

   #138592

   Purpose

   Expresses NatL2-K418A in E.coli

   Depositor

   James Naismith

   Article

   Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

   Insert

   phenylacetate-coenzyme A ligase
   

   Use

   Tags

   6-His

   Expression

   Bacterial

   Mutation

   changed Lysine 418 to Alanine

   Promoter

   T7

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDK580

   Plasmid

   #134205

   Purpose

   Baculovirus expresion of CENP-L and CENP-N

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

   Insert

   CENP-L and His-CENP-N
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKM412

   Plasmid

   #134213

   Purpose

   Baculovirus expression of CENP-P and CENP-O

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

   Insert

   his-CENP-P + O
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDC24

   Plasmid

   #131368

   Purpose

   expression of MEG-3Cterm (aa545-862) in C. elegans

   Depositor

   Geraldine Seydoux

   Article

   Smith et al Elife. 2016 Dec 3;5. pii: 21337. doi: 10

   Insert

   meg-3 (aa545-862) (meg-3 Nematode)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDC21

   Plasmid

   #131367

   Purpose

   expression of MEG-3IDR (aa1-544) in C. elegans

   Depositor

   Geraldine Seydoux

   Article

   Smith et al Elife. 2016 Dec 3;5. pii: 21337. doi: 10

   Insert

   meg-3 (aa1-544) (meg-3 Nematode)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBAD-MTX_Snifit

    Plasmid

    #119043

    Purpose

    Plasmid encoding the bacterial expression of the control Snifit-MTX (FRET semi-synthetic biosensor)

    Depositor

    Kai Johnsson

    Article

    Xue et al J Am Chem Soc. 2016 Apr 27;138(16):5258-

    Insert

    MTX_Snifit
    

    Use

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    R44L H45Q

    Promoter

    pBAD

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET21d-colEdes7/Imdes7

    Plasmid

    #107115

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des7

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes7/Imdes7
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET21d-colEdes8/Imdes8

    Plasmid

    #107116

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des8

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes8/Imdes8
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET21d-colEdes9/Imdes9

    Plasmid

    #107117

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des9

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes9/Imdes9
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET21d-colEdes10/Imdes10

    Plasmid

    #107118

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des10

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes10/Imdes10
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET21d-colEdes11/Imdes11

    Plasmid

    #107119

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des11

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes11/Imdes11
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET21d-colEdes12/Imdes12

    Plasmid

    #107120

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des12

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes12/Imdes12
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET21d-colEdes13/Imdes13

    Plasmid

    #107121

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des13

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes13/Imdes13
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET21d-colEdes14/Imdes14

    Plasmid

    #107122

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des14

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes14/Imdes14
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET21d-colEdes15/Imdes15

    Plasmid

    #107123

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des15

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes15/Imdes15
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET21d-colEdes16/Imdes16

    Plasmid

    #107124

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des16

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes16/Imdes16
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET21d-colEdes17/Imdes17

    Plasmid

    #107125

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des17

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes17/Imdes17
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET21d-colEdes18/Imdes18

    Plasmid

    #107126

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des18

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes18/Imdes18
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET21d-colEdes1/Imdes1

    Plasmid

    #107108

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des1

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes1/Imdes1
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET21d-colEdes2/Imdes2

    Plasmid

    #107109

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des2

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes2/Imdes2
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET21d-colEdes3/Imdes3

    Plasmid

    #107110

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des3

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes3/Imdes3
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET21d-colEdes3.5/Imdes3.5

    Plasmid

    #107111

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des3.5

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes3.5/Imdes3.5
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET21d-colEdes4/Imdes4

    Plasmid

    #107112

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des4

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes4/Imdes4
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET21d-colEdes5/Imdes5

    Plasmid

    #107113

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des5

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes5/Imdes5
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET21d-colEdes6/Imdes6

    Plasmid

    #107114

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des6

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes6/Imdes6
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCDB325

    Plasmid

    #113669

    Purpose

    expresses Ulp1_R1 in Escherichia coli

    Depositor

    Christopher Bahl

    Article

    Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

    Insert

    Ulp1_R1
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    contains solubility enhancing mutations

    Promoter

    T7

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. full length png in pGEX4t

    Plasmid

    #112974

    Purpose

    For protein expression and purification of full-length Drosophila png

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2017 May 30;6. doi: 10.7554/eLife

    Insert

    png (png Fly)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET30a-RAMA

    Plasmid

    #112226

    Purpose

    Expresses RAMA domain from murine MPND

    Depositor

    Douglas Feldman

    Article

    Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

    Insert

    MPND (Mpnd Mouse)
    

    Use

    Tags

    Flag and HIs S-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pTH861-gst-optec P210T

    Plasmid

    #89595

    Purpose

    Plasmid for bacterial expression of a His-tagged P210T mutant of GST encoded by preferred codons

    Depositor

    Tobias von der Haar

    Article

    Jossé et al bioRxiv 200006

    Insert

    glutathione-S transferase P210T mutant, codon-optimised for bacterial expression
    

    Use

    Tags

    8xhistidine

    Expression

    Bacterial

    Mutation

    changed proline 210 to threonine

    Promoter

    T7

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pTH860-gst-optec A180Q

    Plasmid

    #89594

    Purpose

    Plasmid for bacterial expression of a His-tagged A180Q mutant of GST encoded by preferred codons

    Depositor

    Tobias von der Haar

    Article

    Jossé et al bioRxiv 200006

    Insert

    glutathione-S transferase A180Q mutant, codon-optimised for bacterial expression
    

    Use

    Tags

    8xhistidine

    Expression

    Bacterial

    Mutation

    changed alanine 180 to glutamine

    Promoter

    T7

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTH858-gst-optec V156E

    Plasmid

    #89592

    Purpose

    Plasmid for bacterial expression of a His-tagged V156E mutant of GST encoded by preferred codons

    Depositor

    Tobias von der Haar

    Article

    Jossé et al bioRxiv 200006

    Insert

    glutathione-S transferase V156E mutant, codon-optimised for bacterial expression
    

    Use

    Tags

    8xhistidine

    Expression

    Bacterial

    Mutation

    changed valine 156 to glutamic acid

    Promoter

    T7

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pTH859-gst-optec Y172M

    Plasmid

    #89593

    Purpose

    Plasmid for bacterial expression of a His-tagged Y172M mutant of GST encoded by preferred codons

    Depositor

    Tobias von der Haar

    Article

    Jossé et al bioRxiv 200006

    Insert

    glutathione-S transferase Y172M mutant, codon-optimised for bacterial expression
    

    Use

    Tags

    8xhistidine

    Expression

    Bacterial

    Mutation

    changed tyrosine 172 to methionine

    Promoter

    T7

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET28-Metavinculin tail

    Plasmid

    #107152

    Purpose

    bacterial expression

    Depositor

    Tina Izard

    Article

    Chinthalapudi et al Proc Natl Acad Sci U S A. 2016 Aug 23;11

    Insert

    metavinculin (VCL Human)
    

    Use

    Tags

    (His)8

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. P415 δCLD+Ret2 2α

    Plasmid

    #73878

    Purpose

    Swapped helices

    Depositor

    Blanche Schwappach

    Article

    Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

    Insert

    δCLD+Ret2 2α
    

    Use

    Tags

    His

    Expression

    Yeast

    Mutation

    Promoter

    Met25

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. P415 Ret2LD+δC2α

    Plasmid

    #73877

    Purpose

    Swapped helices

    Depositor

    Blanche Schwappach

    Article

    Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

    Insert

    Ret2LD+δC2α
    

    Use

    Tags

    His

    Expression

    Yeast

    Mutation

    Promoter

    Met25

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. p415 CHI-15

    Plasmid

    #73884

    Purpose

    Chimaera 15

    Depositor

    Blanche Schwappach

    Article

    Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

    Insert

    CHI-15
    

    Use

    Tags

    His

    Expression

    Yeast

    Mutation

    Promoter

    Met25

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge