We narrowed to 27,276 results for: Tat

1. MBP-hnRNPA2_FL_WT

   Plasmid

   #98662

   Purpose

   Expresses MBP-tagged full length hnRNPA2

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

   Insert

   hnRNPA2 (HNRNPA2B1 Human)
   

   Use

   Tags

   6xHis-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1-Flag-mRIPK1-S321E

   Plasmid

   #115340

   Purpose

   Mammalian expression of Flag-mRIPK1-S321E

   Depositor

   Junying Yuan

   Article

   Geng et al Nat Commun. 2017 Aug 25;8(1):359. doi: 1

   Insert

   RIPK1 (Ripk1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S321E

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBP Omomer

   Plasmid

   #113169

   Purpose

   Retroviral vector for expressing Omomer (tamoxifen inducible Omomyc), by DNA transfection or retroviral infection.

   Depositor

   Sergio Nasi

   Article

   Soucek et al Cancer Res. 2002 Jun 15;62(12):3507-10.

   Insert

   Omomer: a fusion contruct of Omomyc and MER (a mutant estrogen receptor, reponsive to tamoxifen) (MYC Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   E57T, E64I, R70Q, R71N

   Promoter

   Available since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. piRFP670-N1-GalT

   Plasmid

   #87325

   Purpose

   To express GalT-iRFP670 in mammalian cells, GalT encodes the 1-81 amino acids from β1,4-galactosyltransferase

   Depositor

   Lei Lu

   Article

   Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15

   Insert

   1-81 amino acids from β1,4-galactosyltransferase (B4GALT1 Human)
   

   Use

   Tags

   iRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. His-Precission-NEDD4L_C942S_pCDNA3.1(+)

   Plasmid

   #187729

   Purpose

   Express the catalytically inactive NEDD4L FL in mammalian cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L FL with C874 mutated to a serine (NEDD4L Human)
   

   Use

   Tags

   His-HRV 3C site

   Expression

   Mammalian

   Mutation

   Cys874 mutated to a serine

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. ABE7.10-F148A

   Plasmid

   #132946

   Purpose

   Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.

   Depositor

   Hui Yang

   Article

   Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:

   Insert

   ABE7.10(F148A)
   

   Use

   CRISPR; Base editor

   Tags

   Expression

   Mammalian

   Mutation

   ABE7.10(F148A)

   Promoter

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUC19-U6-BsmBI_cassette-Nme/Nme2Cas9-sgRNA (KAC32)

   Plasmid

   #133794

   Purpose

   U6 promoter sgRNA entry vector used for all NmeCas9 or Nme2Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Amrani et al. Genome Biology 2018

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   NmeCas9 and Nme2Cas9 sgRNA entry vector
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   sgRNA architecture from Amrani et al. Genome Biol…

   Promoter

   U6

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. HiBiT-FZD4

   Plasmid

   #195842

   Purpose

   Expresses human FZD4 with an N-terminal HiBiT

   Depositor

   Gunnar Schulte

   Article

   Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

   Insert

   FZD4 (FZD4 Human)
   

   Use

   Tags

   HiBiT

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pOTTC385 - pAAV CMV-IE IRES EGFP

   Plasmid

   #102936

   Purpose

   An AAV vector that expresses IRES EGFP under the CMV-IE promoter

   Depositor

   Brandon Harvey

   Article

   Nelson et al Neuroscience. 2019 Jun 23;413:77-85. doi

   Insert

   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-IE

   Available since

   Sept. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBABE-hygro-BAP1-HA

    Plasmid

    #154020

    Purpose

    Mammalian Expression of HA-tagged Wild-Type BAP1

    Depositor

    Samuel Peña-Llopis

    Article

    Niersch et al iScience. 2021 Feb 12;24(3):102173. doi:

    Insert

    BAP1 (BAP1 Human)
    

    Use

    Retroviral

    Tags

    Hemagglutinin (HA)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCIneo-AR-S256A

    Plasmid

    #171227

    Purpose

    Mammalian expression of un-tagged human androgen receptor phosphorylation mutant: AR-Ser256Ala (AR-S256A)

    Depositor

    Steven Balk

    Article

    Chen et al Proc Natl Acad Sci U S A. 2006 Oct 24;10

    Insert

    human androgen receptor Ser256Ala mutant (AR Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    AR-Ser256Ala (AR-S256A)

    Promoter

    CMV

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCIneo-AR-S308A

    Plasmid

    #171229

    Purpose

    Mammalian expression of un-tagged human androgen receptor phosphorylation mutant: AR-Ser308Ala (AR-S308A)

    Depositor

    Steven Balk

    Article

    Chen et al Proc Natl Acad Sci U S A. 2006 Oct 24;10

    Insert

    human androgen receptor Ser308Ala mutant (AR Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    AR-Ser308Ala (AR-S308A)

    Promoter

    CMV

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCIneo-AR-S90A

    Plasmid

    #171225

    Purpose

    Mammalian expression of un-tagged human androgen receptor phosphorylation mutant: AR-Ser90Ala (AR-S90A)

    Depositor

    Steven Balk

    Article

    Chen et al Proc Natl Acad Sci U S A. 2006 Oct 24;10

    Insert

    human androgen receptor Ser90Ala mutant (AR Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    AR-Ser90Ala (AR-S90A)

    Promoter

    CMV

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti-dCas9-T2A-GFP

    Plasmid

    #193781

    Purpose

    Tricistronic expression of dCas9-EGFP-BSD

    Depositor

    James Eshleman

    Article

    Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

    Insert

    dead Cas9
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    In frame with existing Cas9 promoter

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HiBiT-FZD2

    Plasmid

    #195840

    Purpose

    Expresses human FZD2 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD2 (FZD2 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. psiCHECK2-Eef2TOPm-RLCP-HCVIRES-FL

    Plasmid

    #233629

    Purpose

    Expression of bicistronic reporter with mutated TOP motif

    Depositor

    Shintaro Iwasaki

    Article

    Shichino et al Nat Struct Mol Biol. 2024 Oct;31(10):155

    Insert

    Promoter and 5'UTR of Eef2 gene with mutation in TOP motif and HCV IRES (Eef2 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    AGAAGG in TOP motif

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1(+)_Granulin 4-no linker

    Plasmid

    #176925

    Purpose

    express Granulin 4 without linker 5 in mammalian cells

    Depositor

    Thomas Kukar

    Article

    Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

    Insert

    Granulin 4 (GRN Human)
    

    Use

    Tags

    Twin-Strep and FLAG tags encoded after Signal Pep…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pWAP-HGF

    Plasmid

    #83503

    Purpose

    Generation of transgenic mice overexpressing the HGF under the control of the WAP gene promoter

    Depositor

    Marta Gallego, Lothar Hennighausen

    Article

    Gallego et al Oncogene. 2003 Nov 20;22(52):8498-508.

    Insert

    HGF (Hgf Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    also contains beta-globin sequences from pUC198

    Promoter

    mouse WAP (whey acidic protein)

    Available since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLV V5-lamin A

    Plasmid

    #175107

    Purpose

    Lentiviral expression of V5-tagged mouse Lmna

    Depositor

    Larry Gerace

    Article

    Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

    Insert

    Lmna (Lmna Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5/FRT/TO V5 DNAJA2

    Plasmid

    #19519

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DnaJ (Hsp40) homolog, subfamily A, member 2 (DNAJA2 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. AAVS1-A2UCOE-EF1a-hCD46-P2A-HLA-G SCT-T2A-hCD47-E2A-hCD59-F2A-neo-AAVS1

    Plasmid

    #205443

    Purpose

    human AAVS1-targeting plasmid for expression of iCasp9, HLA-E SCT, human and CD55

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    hCD46-HLA-G SCT-hCD47-hCD59
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLX302_HOXB13

    Plasmid

    #70089

    Purpose

    Expression of human HOXB13 from CMV promoter, puromycin selection marker

    Depositor

    William Hahn

    Article

    Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

    Insert

    HOXB13 (HOXB13 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCS2_IRES2_CyOFP1_KCNQ2 var4

    Plasmid

    #173155

    Purpose

    For mammalian expression of KCNQ2 and creating variants

    Depositor

    Al George

    Article

    Simkin et al Elife. 2021 Feb 5;10. pii: 64434. doi: 1

    Insert

    KCNQ2 (KCNQ2 Human)
    

    Use

    Tags

    IRES2-CyOFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Simian CMV IE94

    Available since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET-EIN-AlkBH5(66-292)

    Plasmid

    #125732

    Purpose

    Plasmid with EIN as a solubility and expression enhancing tag to increase production of hard to express proteins such as AlkBH5 and TEV cleaving site for the removal of the fusion tag.

    Depositor

    Vincenzo Venditti

    Article

    Khatiwada et al Protein Expr Purif. 2019 Nov 15;167:1055

    Insert

    AlkBH5(66-292) (ALKBH5 Human)
    

    Use

    Tags

    6xHis and EIN

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-hSyn-GRAB_SST1.0

    Plasmid

    #208665

    Purpose

    Expresses the genetically-encoded fluorescent somatostatin (SST) sensor GRAB_SST1.0 in neurons

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based somatostatin (SST) sensor GRAB_SST1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. cpVenus-Gγ2

    Plasmid

    #69626

    Purpose

    The heterotrimeric G-protein subunit, Gγ2, tagged with circular permutated Venus (cp173V).

    Depositor

    Dorus Gadella

    Article

    van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

    Insert

    cp173Venus-Ggamma2 (GNG2 Synthetic)
    

    Use

    Tags

    ciruclar permutated Venus (cp173V)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1/FGFR2myc

    Plasmid

    #33248

    Depositor

    Alka Mansukhani, Claudio Basilico

    Article

    Mansukhani et al J Cell Biol. 2000 Jun 12. 149(6):1297-30

    Insert

    FGFR2 (Fgfr2 Mouse)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    KpnI site introduced at stop codon of FGFR2 to be…

    Promoter

    CMV

    Available since

    Dec. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

28. pLJM1 flag-cFLIP

    Plasmid

    #211529

    Purpose

    Expresses flag-tagged cFLIP (CFLAR)

    Depositor

    Richard Possemato

    Article

    Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

    Insert

    cFLIP (CFLAR Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pmCherry-Cep290

    Plasmid

    #27380

    Depositor

    Joseph Gleeson

    Article

    Valente et al Nat Genet. 2006 Jun . 38(6):623-5.

    Insert

    Cep290 (Cep290 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    please see depositor comments below

    Promoter

    Available since

    June 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. EXT1- D164A-pLJC2-3XFLAG

    Plasmid

    #215828

    Purpose

    Ectopic expression of catalytically inactive mutant (EXT1-D164A-FLAG) in mammalian cells

    Depositor

    Yoav Shaul

    Article

    Solaimuthu et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

    Insert

    Exostosin glycosyltransferase 1 Mutant (EXT1 Human)
    

    Use

    Lentiviral

    Tags

    3X Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLPC-S22A-Progerin

    Plasmid

    #69065

    Purpose

    encodes a Progerin mutant where serine 22 is mutated to an alanine

    Depositor

    Gerardo Ferbeyre

    Article

    Moiseeva et al Cell Cycle. 2015 Aug 3;14(15):2408-21. d

    Insert

    Progerin (LMNA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Progerin with serine 22 mutated to alanine

    Promoter

    CMV

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

32. pLV-EF1A-LivePAR(Y107A)-Hygro

    Plasmid

    #176073

    Purpose

    EGFP fused to the C-terminus of a WWE domain containing the mutation Tyr107Ala & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    LivePAR (Y107A)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Point mutation to convert Try107 to Ala

    Promoter

    EF1A

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3 proHB-EGF CS

    Plasmid

    #11601

    Depositor

    Rosalyn Adam

    Article

    Adam et al Endocrinology. 2002 Dec . 143(12):4599-6

    Insert

    HB-EGF (HBEGF Human)
    

    Use

    Tags

    His and myc

    Expression

    Mammalian

    Mutation

    Constitutively secreted HB-EGF: C-terminal amino …

    Promoter

    Available since

    April 26, 2006

    Availability

    Academic Institutions and Nonprofits only

34. pCS2plus-mCerulean 156-239-GGGS

    Plasmid

    #162616

    Purpose

    Allows for transcription of control mCerulean fluorophore half for injection into zebrafish embryos for BiFC assays

    Depositor

    Marco Morsch

    Article

    Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

    Insert

    mCerulean
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    156-239

    Promoter

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pShuttle CMV RGECO-TnT

    Plasmid

    #124643

    Purpose

    Myofilament localised red genetically encoded calcium sensor in mammalian expression vector used for AdEasy adenoviral DNA recombination.

    Depositor

    Matthew Daniels, Paul Robinson, Hugh Watkins

    Article

    Sparrow et al Circ Res. 2019 Feb 8. doi: 10.1161/CIRCR

    Insert

    RGECO-TnT (TNNT2 Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLV[Exp]-Puro-EF-1α>TurboRFP(ns):T2A:{H2B-RFP}

    Plasmid

    #201120

    Purpose

    RFP expression in cells under EF-1α promoter; Histone-bound, nuclear

    Depositor

    Ori Bar-Nur

    Article

    Zvick et al Stem Cell Reports. 2022 Sep 13;17(9):194

    Insert

    TurboRFP; H2B-RFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    No

    Promoter

    Available since

    May 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. LentiV_Neo_POU2F3_CR

    Plasmid

    #122175

    Purpose

    Lentiviral expression plasmid of human POU2F3 cDNA (CRISPR-resistant silent mutation) with neomycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Huang et al Genes Dev. 2018 Jul 1;32(13-14):915-928.

    Insert

    POU2F3 (POU2F3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    3 silent mutations for gRNA resistance (882g-c, 8…

    Promoter

    EFS

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRK5-Myc-PSK1-beta

    Plasmid

    #197121

    Purpose

    Expression of MYC-tagged PSK1-beta in mammalian cells

    Depositor

    Jonathan Morris

    Article

    Zihni et al J Biol Chem. 2007 Mar 2;282(9):6484-93.

    Insert

    TAOK2 variant beta (aa1-1049) (TAOK2 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Wild Type variant beta

    Promoter

    SV40

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3-myc-his-BACH1 K52R

    Plasmid

    #17643

    Depositor

    Ronny Drapkin

    Article

    Cantor et al Proc Natl Acad Sci U S A. 2004 Feb 24. 1

    Insert

    BACH1 (BRIP1 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    The lysine residue at position 52 was changed to …

    Promoter

    Available since

    April 4, 2008

    Availability

    Academic Institutions and Nonprofits only

40. pcDNA5/FRT/TO GFP DNAJB6b

    Plasmid

    #19501

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB6b (DNAJB6 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge