We narrowed to 22,191 results for: his

1. p40 D293K

   Plasmid

   #19017

   Depositor

   Michael Yaffe

   Article

   Bissonnette et al J Biol Chem. 2008 Jan 25. 283(4):2108-19

   Insert

   p40phox (NCF4 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   D293K

   Promoter

   Available Since

   Aug. 25, 2008

   Availability

   Academic Institutions and Nonprofits only

2. p40 R58Q/E259A

   Plasmid

   #19020

   Depositor

   Michael Yaffe

   Article

   Bissonnette et al J Biol Chem. 2008 Jan 25. 283(4):2108-19

   Insert

   p40phox (NCF4 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   R58Q/E259A

   Promoter

   Available Since

   Aug. 25, 2008

   Availability

   Academic Institutions and Nonprofits only

3. p40 D293K/E259A

   Plasmid

   #19021

   Depositor

   Michael Yaffe

   Article

   Bissonnette et al J Biol Chem. 2008 Jan 25. 283(4):2108-19

   Insert

   p40phox (NCF4 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   D293K/E259A

   Promoter

   Available Since

   Aug. 25, 2008

   Availability

   Academic Institutions and Nonprofits only

4. p40 W207R/E259A

   Plasmid

   #19022

   Depositor

   Michael Yaffe

   Article

   Bissonnette et al J Biol Chem. 2008 Jan 25. 283(4):2108-19

   Insert

   p40phox (NCF4 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   W207R/E259A

   Promoter

   Available Since

   Aug. 25, 2008

   Availability

   Academic Institutions and Nonprofits only

5. p40 E259A

   Plasmid

   #19023

   Depositor

   Michael Yaffe

   Article

   Bissonnette et al J Biol Chem. 2008 Jan 25. 283(4):2108-19

   Insert

   p40phox (NCF4 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   E259A

   Promoter

   Available Since

   Aug. 25, 2008

   Availability

   Academic Institutions and Nonprofits only

6. PA RdgC_pET21a

   Plasmid

   #14927

   Depositor

   SeWon Suh

   Article

   Ha et al Nucleic Acids Res. 2007;35(8):2671-81. d

   Insert

   Recombination-associated protein rdgC
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 29, 2007

   Availability

   Academic Institutions and Nonprofits only

7. Ypd1p-pET22b

   Plasmid

   #12655

   Depositor

   SeWon Suh

   Article

   Song et al J Mol Biol. 1999 Nov 5. 293(4):753-61.

   Insert

   YPD1 protein (YPD1 Budding Yeast)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pET21a(+)-PA PdxB

   Plasmid

   #12668

   Depositor

   SeWon Suh

   Article

   Ha et al Acta Crystallograph Sect F Struct Biol C

   Insert

   Erythronate-4-phosphate dehydrogenase
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET-21a(+) SurE D8A

   Plasmid

   #12685

   Depositor

   SeWon Suh

   Article

   Lee et al Nat Struct Biol. 2001 Sep . 8(9):789-94.

   Insert

   SurE (TM1662 )
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   changed Aspartate 8 to Alanine

   Promoter

   Available Since

   Jan. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

10. L284F Cus2p

    Plasmid

    #73059

    Purpose

    S. cerevisiae Cus2p gene with L284F Mutation

    Depositor

    Aaron Hoskins

    Article

    Rodgers et al RNA. 2015 Dec 2.

    Insert

    CUS2p (CUS2 Budding Yeast)
    

    Use

    Tags

    6x His tag fused to TEV cleavage site

    Expression

    Bacterial and Yeast

    Mutation

    Leucine 284 to Phenylalanine

    Promoter

    T7

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. D282N Cus2p

    Plasmid

    #73060

    Purpose

    S. cerevisiae Cus2p gene with D282N Mutation

    Depositor

    Aaron Hoskins

    Article

    Rodgers et al RNA. 2015 Dec 2.

    Insert

    CUS2p (CUS2 Budding Yeast)
    

    Use

    Tags

    6x His tag fused to TEV cleavage site

    Expression

    Bacterial and Yeast

    Mutation

    Asp 282 to Asn

    Promoter

    T7

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMSCV-MerCreMer-hygro

    Plasmid

    #188982

    Purpose

    Retroviral expression of tamoxifen-inducible Cre recombinase

    Depositor

    William Kaelin

    Article

    Shi et al Cancer Cell. 2022 Sep 12;40(9):939-956.e

    Insert

    Tamoxifen-inducible Cre recombinase
    

    Use

    Cre/Lox and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLBCX-Large-T-antigenK1 mutant

    Plasmid

    #189978

    Purpose

    Retroviral vector used to immortalize human cells

    Depositor

    William Kaelin

    Article

    Shi et al Cancer Cell. 2022 Sep 12;40(9):939-956.e

    Insert

    Large-T-antigenK1 mutant
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV2-sgTrp53-sgAtrx-EFS-Cre

    Plasmid

    #189977

    Purpose

    Expresses Cre cDNA and sgRNAs targeting murine Trp53 and Atrx

    Depositor

    William Kaelin

    Article

    Shi et al Cancer Cell. 2022 Sep 12;40(9):939-956.e

    Insert

    sgTrp53, sgAtrx, Cre recombinase
    

    Use

    AAV, Cre/Lox, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. sortase A pentamutant (eSrtA) in pET29

    Plasmid

    #75144

    Purpose

    evolved sortase A (eSrtA) pentamutant with improved kinetics and activity

    Depositor

    David Liu

    Article

    Chen et al Proc Natl Acad Sci U S A. 2011 Jul 12;10

    Insert

    sortase A pentamutant
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    P94R, D160N, D165A, K190E, K196T. Deleted amino a…

    Promoter

    T7

    Available Since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET21a-Streptavidin-Alive

    Plasmid

    #20860

    Purpose

    Alive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.

    Depositor

    Alice Ting

    Article

    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

    Insert

    Streptavidin-Alive
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Wt gene

    Promoter

    Available Since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Mfn2-Myc

    Plasmid

    #23213

    Depositor

    David Chan

    Article

    Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

    Insert

    Mfn2 (Mfn2 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET15b-hnRNP-H

    Plasmid

    #23020

    Depositor

    Douglas Black

    Article

    Chou et al Mol Cell Biol. 1999 Jan . 19(1):69-77.

    Insert

    hnRNP-H (HNRNPH1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTriExRhoA2G

    Plasmid

    #40176

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    RhoA Biosensor
    

    Use

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGM1202

    Plasmid

    #69615

    Purpose

    inducible gene expression in Streptomyces, C-terminal His-tag encoding sequence

    Depositor

    Günther Muth

    Article

    Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

    Inserts

    aac
    tsr
    rep
    sso
    TipA promoter
    dso
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PET-28a-YTH domain of YTHDC2

    Plasmid

    #228779

    Purpose

    Protein expression plasmid for YTH domain of YTHDC2 in E.Coli.

    Depositor

    Huiqing Zhou

    Article

    Wang et al RSC Chem Biol. 2024 Jul 25;5(9):914-923.

    Insert

    YTH domain of YTHDC2 (YTHDC2 Human)
    

    Use

    Tags

    6*His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. SENP2M497A

    Plasmid

    #61088

    Purpose

    cleave the SUMO off when use PETSUMO vector for express fusion protein

    Depositor

    Cynthia Wolberger

    Article

    Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033

    Insert

    SENP2M497A catalytic domain (SENP2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    M497A; Catalytic domain is residues 364-589

    Promoter

    T7

    Available Since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

23. pBad-sfGFP 150TAG

    Plasmid

    #85483

    Purpose

    Arabinose inducible E. coli codon optimized superfolder GFP with TAG stop codon at position 150

    Depositor

    Ryan Mehl

    Article

    Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

    Insert

    superfolder GFP 150TAG
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    150TAG (amino acid 150 mutated to stop codon)

    Promoter

    Arabinose

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBad-LgBiT-PhoCl1-SmBiT-MBP

    Plasmid

    #164034

    Purpose

    luciferase-based PhoCl screening construct

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    LgBiT-PhoCl1-SmBiT-MBP
    

    Use

    Tags

    His tag, T7 tag, Xpress Tag

    Expression

    Bacterial

    Mutation

    Promoter

    araBad promotor

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET-Expotin 1

    Plasmid

    #42047

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    Exportin-1 (XPO1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA flag PPAR gamma

    Plasmid

    #8895

    Purpose

    Mammalian expression of flag-tagged PPAR gamma

    Depositor

    Bruce Spiegelman

    Article

    Hauser et al J Biol Chem. 2000 Jun 16. 275(24):18527-

    Insert

    PPAR gamma (Pparg Mouse)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBADmod1-linker2-gamS

    Plasmid

    #45833

    Depositor

    Richard Murray, Vincent Noireaux

    Article

    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

    Insert

    gamS
    

    Use

    Synthetic Biology

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHM6-Q23

    Plasmid

    #40263

    Depositor

    David Rubinsztein

    Article

    Narain et al J Med Genet. 1999 Oct;36(10):739-46.

    Insert

    HTT partial exon 1 Q23 (HTT Human)
    

    Use

    Tags

    6x His and HA

    Expression

    Mammalian

    Mutation

    expanded poly-Q

    Promoter

    CMV

    Available Since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLentiRhoA2G

    Plasmid

    #40179

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    RhoA Biosensor
    

    Use

    Lentiviral

    Tags

    His-Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pQTEV-PFDN2

    Plasmid

    #31329

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    prefoldin 2 (PFDN2 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET28a T7pCONS TIR-2 sfGFP

    Plasmid

    #154464

    Purpose

    pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

    Depositor

    Daniel Daley

    Article

    Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

    Insert

    super folder GFP
    

    Use

    Tags

    His-thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMSP1E3

    Plasmid

    #20064

    Depositor

    Stephen Sligar

    Article

    Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

    Insert

    MSP1E3 (APOA1 synthetic gene)
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    "extended" MSP1; contains repeats of he…

    Promoter

    Available Since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pQE30 PTBP1 isoform1

    Plasmid

    #108591

    Purpose

    Recombinant His tagged protein production

    Depositor

    Chris Smith

    Article

    Wollerton et al RNA. 2001 Jun;7(6):819-32.

    Insert

    PTBP1 isoform 1 (PTBP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    isoform 1 (isoform c)

    Promoter

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHL-sec

    Plasmid

    #99845

    Purpose

    Mammalian expression with secretion signal sequence

    Depositor

    Edith Yvonne Jones

    Article

    Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

    Insert

    ephrin b2 (EFNB2 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. NbV5-GFP2

    Plasmid

    #201477

    Purpose

    BRET2 detection of V5-tagged protein

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    NbV5-GFP2
    

    Use

    Tags

    GFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pKS1

    Plasmid

    #84079

    Purpose

    Expresses the non-toxic fragment C of tetanus toxin

    Depositor

    Neil Fairweather

    Article

    Sinha et al Mol Microbiol. 2000 Sep;37(5):1041-51.

    Insert

    Tetanus neurotoxin fragment C
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. LM670

    Plasmid

    #191552

    Purpose

    LM670_MSG-AGGA-promoter-ccdb-TTCC-GShis

    Depositor

    David Baker

    Article

    Wicky et al Science. 2022 Oct 7;378(6615):56-61. doi

    Insert

    ccdb
    

    Use

    Tags

    HIS-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLenti-Cdc42-2G

    Plasmid

    #68813

    Purpose

    FRET-based biosensor reporting on endogenous Cdc42 activation (for Lentivirus production)

    Depositor

    Olivier Pertz

    Article

    Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

    Insert

    Cdc42 activation reporter
    

    Use

    Lentiviral

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCDB302

    Plasmid

    #113673

    Purpose

    expresses Cth SUMO protease in Escherichia coli

    Depositor

    Christopher Bahl

    Article

    Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

    Insert

    Cth SUMO protease
    

    Use

    Tags

    10-His

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-934

    Promoter

    T7

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. NbAlfa-GFP2

    Plasmid

    #201485

    Purpose

    BRET2 detection of ALFA-tagged protein

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    NbAlfa-GFP2
    

    Use

    Tags

    GFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge