We narrowed to 10,449 results for: T7
-
Plasmid#194665PurposeProtein production of the split HaloTag based calcium recorder Caprola_04 in bacteriaDepositorInsertCaprola_04
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_06
Plasmid#194667PurposeProtein production of the split HaloTag based calcium recorder Caprola_06 in bacteriaDepositorInsertCaprola_06
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_07
Plasmid#194668PurposeProtein production of the split HaloTag based calcium recorder Caprola_07 in bacteriaDepositorInsertCaprola_07
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_08
Plasmid#194669PurposeProtein production of the split HaloTag based calcium recorder Caprola_08 in bacteriaDepositorInsertCaprola_08
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_10
Plasmid#194671PurposeProtein production of the split HaloTag based calcium recorder Caprola_10 in bacteriaDepositorInsertCaprola_10
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_11
Plasmid#194672PurposeProtein production of the split HaloTag based calcium recorder Caprola_11 in bacteriaDepositorInsertCaprola_11
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_12
Plasmid#194673PurposeProtein production of the split HaloTag based calcium recorder Caprola_12 in bacteriaDepositorInsertCaprola_12
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_02
Plasmid#194663PurposeProtein production of the split HaloTag based calcium recorder Caprola_02 in bacteriaDepositorInsertCaprola_02
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_03
Plasmid#194664PurposeProtein production of the split HaloTag based calcium recorder Caprola_03 in bacteriaDepositorInsertCaprola_03
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1(G137I)
Plasmid#133272PurposeX. laevis expression vector. It will generate the mouse TREK-1 channelDepositorInsertPotassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)
UseOocyte expressionTagsExpressionMutationG137IPromoterT7Available sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
LARP1_323-509
Plasmid#209800PurposeBacterial expression of human LARP1 La-moduleDepositorInsertLARP1 La-module (LARP1 Human)
UseTags6xHisExpressionBacterialMutationaa 323-509 onlyPromoterT7Available sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
LARP1_323-410
Plasmid#209801PurposeBacterial expression of human LARP1 LaM domainDepositorInsertLARP1 LaM (LARP1 Human)
UseTags6xHisExpressionBacterialMutationaa 323-410 onlyPromoterT7Available sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pet28b_His_RAD23A-UBA1
Plasmid#201549PurposeExpresses UBA1 of human RAD23A in E. coliDepositorInsertUV excision repair protein RAD23 homolog A (RAD23A Human)
UseTags6x HisExpressionBacterialMutationExpresses amino acids 160-205 of DNA polymerase i…PromoterT7Available sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 _CTD_6F→A
Plasmid#194269PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with 6F→ADepositorInsertTDP-43 (TARDBP Human, Synthetic)
UseTags6XHistagExpressionBacterialMutationF276A, F283A, F289A, F367A, F397A, F401APromoterT7Available sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 _CTD_5M→A_CR
Plasmid#194263PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with 5M→A_CRDepositorInsertTDP-43 (TARDBP Human, Synthetic)
UseTags6XHistagExpressionBacterialMutationM322A, M323A, M336A, M337A, M339A mutations in TD…PromoterT7Available sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 _CTD_5M→A_IDR
Plasmid#194264PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Methionines to AlanineDepositorInsertTDP-43 (TARDBP Human, Synthetic)
UseTags6XHistagExpressionBacterialMutationM307A, M311A, M359A, M405A, M414A mutations in TD…PromoterT7Available sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 _CTD_5M→L_IDR
Plasmid#194265PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Methionines to leucineDepositorInsertTDP-43 (TARDBP Human)
UseTags6XHistagExpressionBacterialMutationM307L, M311L, M359L, M405L, M414L mutations in TD…PromoterT7Available sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 _CTD_5M→Y_IDR
Plasmid#194266PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Methionines to tyrosineDepositorInsertTDP-43 (TARDBP Human, Synthetic)
UseTags6XHistagExpressionBacterialMutationM307Y, M311Y, M359Y, M405Y, M414Y mutations in TD…PromoterT7Available sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 _CTD_5A→M_IDR
Plasmid#194267PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Alanines to methionineDepositorInsertTDP-43 (TARDBP Human, Synthetic)
UseTags6XHistagExpressionBacterialMutationA297M, A367M, A381M, A388M, A391M mutations in TD…PromoterT7Available sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-B-HypaR-SpCas9-NLS-6xHis
Plasmid#207388PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertB-HypaR-SpCas9
UseCRISPRTags6xHisExpressionBacterialMutationR661A, N692A, M694A, Q695A, H698A, amino acids 10…PromoterT7Available sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
1BR9-AtWUSSTOP-Δα3
Plasmid#202056PurposeE. coli expression vector for N-ter GFP11 tagged Arabidopsis thaliana WUSCHEL with the third helix in the homeodomain deleted.DepositorInsertAtWUS E. Coli Codon Optimized, third helix of homeodomain deleted (WUS Mustard Weed)
UseTags6xHis and GFP11ExpressionBacterialMutationthird helix of homeodomain deleted (AA82-102)PromoterT7 PromoterAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-B-HeFSpCas9-NLS-6xHis
Plasmid#207392PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertB-HeFSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationN497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A…PromoterT7Available sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-B-HiFi SpCas9-NLS-6xHis
Plasmid#207378PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertB-HiFi SpCas9
UseCRISPRTags6xHisExpressionBacterialMutationR691A, amino acids 1005-1013 replaced with two gl…PromoterT7Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBVboostFGII WPRE 3C-CVB3
Plasmid#202077PurposeBacterial expression of 3C protease from coxsackievirus B3 (CVB3). Contains C-terminal His6-tag.DepositorInsertCVB3 3C protease with HisTag
UseTags6xHis-tagExpressionBacterial, Insect, and Mamm…MutationPromoterpolh, CAG, T7Available sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBVboostFGII WPRE 2A-CVB3
Plasmid#202076PurposeBacterial expression of 2A protease from coxsackievirus B3 (CVB3). Contains C-terminal His6-tag.DepositorInsertCVB3 2A protease with HisTag
UseTags6xHis-tagExpressionBacterial, Insect, and Mamm…MutationPromoterpolh, CAG, T7Available sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR201-Eps8L2_494-546
Plasmid#196531PurposeEntry vector for Gateway with Eps8L2_494-546DepositorInsertEps8L2_494-546 (EPS8L2 Human)
UseTagsExpressionBacterialMutationPromoterCMV promoter; T7 promoterAvailable sinceJuly 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP4C, residues 156-233, no Cys
Plasmid#180642PurposeBacterial expression for CHMP4C C-terminal tail, residues 156-233. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-14.DepositorInsertCHMP4C (CHMP4C Human)
UseTagsHIS-SUMOExpressionBacterialMutationresidues 316-366PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 IST1, residues 315-360, no Cys
Plasmid#180643PurposeBacterial expression of IST1 C-terminal tail, residues 315-360. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-99.DepositorInsertIST1 (IST1 Human)
UseTagsHIS-SUMOExpressionBacterialMutationresidues 315-360PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 KATNA1 MIT residues 1-79
Plasmid#180614PurposeBacterial expression for KATNA1 MIT domains, residues 1-79. Has N-terminal HIS-SUMO tag. Internal ID: WISP19-20.DepositorInsertKATNA1 (KATNA1 Human)
UseTagsHIS-SUMOExpressionBacterialMutationResidues 1-79PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 KATNA1 MIT residues 1-79, V55D
Plasmid#180615PurposeBacterial expression for KATNA1 MIT domains, residues 1-79. Has V55D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-23.DepositorInsertKATNA1 (KATNA1 Human)
UseTagsHIS-SUMOExpressionBacterialMutationResidues 1-79, V55D mutationPromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 KATNAL1 MIT residues 1-87
Plasmid#180618PurposeBacterial expression for KATNAL1 MIT domains, residues 1-87. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-54.DepositorInsertKATNAL1 (KATNAL1 Human)
UseTagsHIS-SUMOExpressionBacterialMutationResidues 1-87PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR201-Eps8L2_367-496
Plasmid#196530PurposeEntry vector for Gateway with Eps8L2_367-496DepositorInsertEps8L2_367-496 (EPS8L2 Human)
UseTagsExpressionBacterialMutationPromoterCMV promoter; T7 promoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR201-Eps8L2_1-183
Plasmid#196527PurposeEntry vector for Gateway with Eps8L2_1-183DepositorInsertEps8L2_1-183 (EPS8L2 Human)
UseTagsExpressionBacterialMutationPromoterCMV promoter; T7 promoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR201-Eps8L2_544-715
Plasmid#196532PurposeEntry vector for Gateway with Eps8L2_544-715DepositorInsertEps8L2_544-715 (EPS8L2 Human)
UseTagsExpressionBacterialMutationPromoterCMV promoter; T7 promoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR201-Eps8L2_181-299
Plasmid#196528PurposeEntry vector for Gateway with Eps8L2_181-299DepositorInsertEps8L2_181-299 (EPS8L2 Human)
UseTagsExpressionBacterialMutationPromoterCMV promoter; T7 promoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-Ctdnep1_C-ter
Plasmid#196525PurposeExpression of Ctdnep1_C-terDepositorInsertCtdnep1-C-ter (CTDNEP1 Human)
UseTagsHisExpressionBacterialMutationNo transmembrane domainPromoterT7 promoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM30-C9ORF78
Plasmid#193367PurposeExpression of C9ORF78 in E.coli with N-terminal His6-GST-tag which is cleavable with TEV proteaseDepositorInsertC9ORF78 (C9orf78 Human)
UseTagsHis6-GST-TEVExpressionBacterialMutationPromoterT7Available sinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet-6xHis-TEV-Nsp8(76-198)
Plasmid#201024PurposeBacterial expression of codon optimized SARS-CoV-2 nsp8 residues 76-198, with an N-terminus His tag followed by a TEV cleavage site.DepositorInsertnon-structural protein 8 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
UseTags6xHis-TEVExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a LZ control-FLAG
Plasmid#199678PurposeBacterial expression of Leucine Zipper (LZ) control-FlagDepositorInsertLeucine zipper control
UseTags6xHis and FlagExpressionBacterialMutationPromoterT7 promoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
DNMT2Δ191-237
Plasmid#198382PurposeBacterial expression plasmid for human DNMT2 deletion mutant; for binding assaysDepositorInsertDNMT2 (TRDMT1 Human)
UseTagsHis-tagExpressionBacterialMutationdeleted amino acids 191-237PromoterT7Available sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only