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171,035 results

Showing: 11651 - 11700 of 171035 results

  3. pBS L30 mRuby3-Rab18

    Plasmid
    #166431
    Purpose
    Rab18 fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.
    Depositor
    Raphael Gaudin
    Insert
    Rab18
    Tags
    mRuby3
    Expression
    Mammalian
    Promoter
    L30 weak promoter
    Available Since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    166431-plasmid-map-sequence-id-333545

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  12. pTYB3-T4gp43(WT)

    Plasmid
    #228203
    Purpose
    Expression in BL21(DE3) of WT T4 DNA polymerase
    Depositor
    Stephen Benkovic
    Insert
    43
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    228203-plasmid-map-sequence-id-472938

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  18. mCherry-Cx43-7

    Plasmid
    #55023
    Purpose
    Localization: Gap Junctions, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Cx43 (Gja1 Rat)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55023-plasmid-map-sequence-id-142375

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  23. pSupAR-MbPylRS(DiZPK)

    Plasmid
    #91705
    Purpose
    express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interest
    Depositor
    P. Chen
    Article
    Zhang et al Nat Chem Biol. 2011 Sep 4;7(10):671-7. d
    Inserts
    pylS
    pylT
    Expression
    Bacterial
    Mutation
    L274A, C313S
    Available Since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    91705-plasmid-map-sequence-id-183278

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  25. AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

    Plasmid
    #60231
    Purpose
    Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Cre recombinase
    EGFP
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA-P2A and EGFP-KASH
    Expression
    Mammalian
    Promoter
    U6 and hSyn
    Available Since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    60231-plasmid-map-sequence-id-417804

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  27. pET151-D-TOPO-PARP14-SΔK

    Plasmid
    #216658
    Purpose
    Expresses multidomain construct of PARP14 with internal SUMO domain (PARP14-SΔK) in bacterial cells.
    Depositor
    Herwig Schüler
    Article
    Chatzicharalampous et al Protein Expr Purif. 2024 Aug 16:106580.
    Insert
    PARP14-SΔK DNA sequence (PARP14 G784-K1808 with internal SUMO-TEV sequence) (PARP14 Human, Synthetic)
    Tags
    6xHis-V5-TEV
    Expression
    Bacterial
    Mutation
    Deletion starting from Q1390 to D1448 and substit…
    Promoter
    T7
    Available Since
    Aug. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    216658-plasmid-map-sequence-id-429340

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  34. pET22b_PstS_2

    Plasmid
    #78199
    Purpose
    Expression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor
    Depositor
    Martin Webb
    Article
    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62
    Insert
    pstS (A17C,A197C)
    Expression
    Bacterial
    Mutation
    A17C; A197C (please see depositor comment below)
    Promoter
    T7
    Available Since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78199-plasmid-map-sequence-id-146049

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  35. eGFP-proα2(I)-G610C

    Plasmid
    #119827
    Purpose
    Expresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and GFP between signal sequence and exon 6
    Depositor
    Sergey Leikin
    Article
    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11
    Insert
    Type I procollagen α2 chain (Col1a2 Mouse)
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Col1a2 exons 2-5 replaced by fluorescent tag, Gly…
    Promoter
    CMV
    Available Since
    Jan. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119827-plasmid-map-sequence-id-233848

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  37. pENTR11 CA NFAT1

    Plasmid
    #11792
    Depositor
    Anjana Rao
    Insert
    NFAT1 (Nfatc2 Mouse)
    Tags
    HA
    Expression
    Bacterial
    Mutation
    Constitutively active: multiple serine to alanine…
    Available Since
    Aug. 7, 2006
    Availability
    Academic Institutions and Nonprofits only
    11792-plasmid-map-sequence-id-229776

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  41. pMN-332

    Plasmid
    #195466
    Purpose
    Lentiviral Integratable Inducible synZiFTR ZF1 + Reporter
    Depositor
    Ahmad Khalil
    Article
    Li et al Science. 2022 Dec 16;378(6625):1227-1234
    Insert
    inverted[8X ZF1 BS - ybTATA - mCherry] - pSFFV - ABI1 - ZF1 - 2A - p65 - PYL1
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195466-plasmid-map-sequence-id-384256

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  46. pCAG-enAsCas12a(E174R/S542R/K548R)-NLS(nuc)-3xHA (AAS848)

    Plasmid
    #107941
    Purpose
    Mammalian expression plasmid for human codon optimized enAsCas12a (enhanced AsCas12a) encoding E174R/S542R/K548R substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized enAsCas12a (E174R/S542R/K548R)
    Tags
    3x HA and NLS (nucleoplasmin)
    Expression
    Mammalian
    Mutation
    E174R, S542R and K548R
    Promoter
    CAG
    Available Since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    107941-plasmid-map-sequence-id-418295

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  47. pET-6xHis-(pos28)GFP

    Plasmid
    #89250
    Purpose
    Expresses GFP with +28 theoretical charge in E.Coli
    Depositor
    David Liu
    Insert
    His6-(pos16)-GFP
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    89250-plasmid-map-sequence-id-176151

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Showing: 11651 - 11700 of 171035 results