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Plasmid#221350PurposeMammalian expression of Anti-Biotin [IPI-M33] Mouse/Rabbit LC kappa; to be used with Anti-Biotin [IPI-M33] heavy chain (Plasmid 221349) to make the antibody.DepositorInsertAnti-Biotin [IPI-M33] Mouse/Rabbit LC kappa
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTipi2.1_Anti-ProC [IPI-HPC-4] Mouse/Rabbit LC kappa
Plasmid#221362PurposeMammalian expression of Anti-ProC [IPI-HPC-4] Mouse/Rabbit LC kappa; to be used with Anti-ProC [IPI-HPC-4] heavy chain (Plasmid 221361) to make the antibody.DepositorInsertAnti-ProC [IPI-HPC-4] Mouse/Rabbit LC kappa
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTipi2.1_Anti-GCN4 [IPI-C11L34] Mouse/Rabbit LC kappa
Plasmid#221354PurposeMammalian expression of Anti-GCN4 [IPI-C11L34] Mouse/Rabbit LC kappa; to be used with Anti-GCN4 [IPI-C11L34] heavy chain (Plasmid 221353) to make the antibody.DepositorInsertAnti-GCN4 [IPI-C11L34] Mouse/Rabbit LC kappa
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
T7opt in pCAGGS
Plasmid#65974PurposeHuman codon-optimized T7 polymeraseDepositorInsertT7-opt
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hATG7wt
Plasmid#87867PurposeExpress a wild type human Atg7/Apg7 in mammalian cellsDepositorInsertATG7 (ATG7 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-hDDB2-WT-V5
Plasmid#167468Purposeectopic expression of hDDB2 in mammalian cellsDepositorInsertDDB2 (DDB2 Human)
UseTags6xHis and V5ExpressionMammalianMutationPromoterCMVAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTP298 His14-Avi-27xGS-SUMOEu-2xGS-anti ALFA tag nanobody
Plasmid#199390PurposeBacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag, biotin acceptor peptide (Avi), and SUMOEu tagDepositorInsertanti-ALFA nanobody
UseTags14xHis-Avi-SUMOEuExpressionBacterialMutationPromoterT5-LacOAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1 hSCN5A
Plasmid#145374Purposeexpresses human Nav1.5 (WT) in mammalian cellsDepositorInsertsodium voltage-gated channel alpha subunit 5 (SCN5A Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
plenti-CAG-IRF4-FLAG-IRES-GFP
Plasmid#107389PurposeLentiviral expression of IRF4-FLAGDepositorInsertIRF4 (IRF4 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCAGAvailable sinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGK-HELLO LV
Plasmid#202001PurposeExpresses HELLO neoantigen in tumor cellsDepositorInsertHELLO
UseLentiviralTagsFLAGExpressionMutationPromoterpGKAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-CRBN-pGK-HYG
Plasmid#107380PurposeMammalian expression of FLAG-CRBNDepositorInsertcereblon (CRBN Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
H2B-mGreenLantern
Plasmid#164464PurposeLocalization of mGreenLantern fluorescent protein to histone 2BDepositorInsertmGreenLantern
UseTagsHuman Histone 2B (D26G and V119I mutations)ExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-mGreenLantern
Plasmid#164469PurposeConstitutive CAG-driven expression of mGreenLantern in animals using AAVDepositorInsertmGreenLantern
UseAAVTagsExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCAGAvailable sinceMarch 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
plenti-UbcP-HA-CRBN-pGK-HYG
Plasmid#107378PurposeLentiviral expression of HA-CRBNDepositorInsertcereblon (CRBN Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterUbcPAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti_SaCRISPR_GFP
Plasmid#118636PurposeTo clone sgRNA for SaCas9 (SauCas9)DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterU6Available sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hfnCas12a_CRATES
Plasmid#176251PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and type IIS restriction site for endonuclease BaeI at the crRNA region that facilitates the generation of mulitplDepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hfnCas12a_Nlux_(-HH)crRNA NR1
Plasmid#176250PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1 without the hammerhead ribozymDepositorInserthumanized fnCas12a
UseCRISPR and Synthetic Biology; Expression in micro…TagsExpressionMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
p425_Cas9_gRNA_LEU_1014a
Plasmid#87407Purposep425_Cas9_gRNA-ARS1014a All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, LEU2 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1014a sequence TTATGTGCGTATTGCTTTCA in yeast chromosome 10.DepositorInsertHuman Optimized S. pyogenes Cas9 and guide RNA targeting ARS1014a
UseCRISPRTagsExpressionMutationPromoterADH1, pTyrosineAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
p426_Cas9_gRNA-YPRCd15c
Plasmid#87404PurposeAll-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting YPRCd15c sequence AATCCGAACAACAGAGCATA in yeast chromosome 14.DepositorInsertHuman Optimized S. pyogenes Cas9 and guide RNA targeting YPRCd15c
UseCRISPRTagsExpressionMutationPromoterADH1, pTyrosineAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR-CLIP-PD-L2
Plasmid#223587PurposeLentiviral expression of human PD-L2 with CLIP tag in mammalian cellsDepositorInsertPD-L2 (PDCD1LG2 Human)
UseLentiviral; mammalian expressionTagsCLIPExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3/TO/CD9-GFP
Plasmid#104402PurposeExpresses CD9 with mEmerald tag (NOT GFP) at the N terminalDepositorInsertCD9 (CD9 Human)
UseLentiviralTagsmEmeraldExpressionMutationPromoterCMVAvailable sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEFIRES-P-ACSL3-mCherry
Plasmid#87158PurposeFluorescent protein targeted to lipid droplets (LDs) from the endoplasmic reticulum (ER)DepositorInsertACSL3 (ACSL3 Human)
UseTagsmCherryExpressionMammalianMutationPromoterEF-1-alphaAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
plenti-UBC- IKZF4-3xHA-pGK-PUR
Plasmid#107384PurposeLentiviral expression of IKZF4-3xHADepositorInsertIKZF4 (IKZF4 Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterUBCAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-mGreenLantern
Plasmid#164468PurposeCre-dependent EF1a-driven expression of mGreenLantern in animals using AAVDepositorInsertmGreenLantern
UseAAVTagsExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterEF1aAvailable sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-flag-YTHDF2
Plasmid#52300Purposefull length YTHDF2 with N-terminal flag tag for mammalian cell expressionDepositorInsertYTHDF2 (YTHDF2 Human)
UseTagsflagExpressionMammalianMutationPromoterT7Available sinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 flag-cFLIP
Plasmid#211529PurposeExpresses flag-tagged cFLIP (CFLAR)DepositorInsertcFLIP (CFLAR Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
p-EGFP-C1-Flag-mFmr1(wt)
Plasmid#87929PurposeExpresses mouse FMRP-EGFP fusion, which can be phosphorylated on ser-499.DepositorInsertFmr1 (Fmr1 Mouse)
UseTagsFlag and eGFPExpressionMammalianMutationNonePromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEx-4T-1-YTHDF3
Plasmid#70088PurposeEncode YTHDF3 isoform a with N-terminal GST tag for E. coli expressionDepositorInsertYTH N(6)-methyladenosine RNA binding protein 3 (YTHDF3 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMXs-ms-Klf2
Plasmid#50786Purposeretroviral expression of mouse Klf2DepositorInsertKlf2 (Klf2 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEx-4T-1-YTHDF1
Plasmid#70087PurposeEncode YTHDF1 with N-terminal GST tag for E. coli expressionDepositorInsertYTH N(6)-methyladenosine RNA binding protein 1 (YTHDF1 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
Mito-mGreenLantern
Plasmid#164461PurposeLocalization of mGreenLantern fluorescent protein to mitochondriaDepositorInsertmGreenLantern
UseTagsHuman COX8a first 29 amino acidsExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT3TS nErCas12an
Plasmid#132642PurposeUsed for in vitro transcription of dual NLS ErCas12a mRNADepositorInsertErCas12a
UseCRISPR; In vitro transcriptionTagsSV40 NLSExpressionMutationPromoterT3Available sinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-ChR2-YFP
Plasmid#204345PurposePiggyBac transposon vector suitable for inserting the ChR2-YFP optogenetic actuator driven by the ubiquitous CAG promoter into the genome of mammalian cell lines.DepositorInsertChR2-YFP
UseTagsYellow fluorescent proteinExpressionMammalianMutationC-terminus (aa315-737) is deleted - PMID: 1461559…PromoterCAGAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGLO-GFP-1UAG
Plasmid#82500PurposeExpresses GFP with 1 UAG codon at the amino acid position 3DepositorInsertGreen Fluorescent Protein with 1 UAG codon
UseTagsExpressionBacterialMutationAdded an UAG codon at the amino acid position 3PromoterAraBADAvailable sinceSept. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
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Hsp70-mplum
Plasmid#45016DepositorInsertmPlum
UseTagsExpressionMammalianMutationPromotermouse Hspa1bAvailable sinceMay 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLV-CDH5-mRuby3-IRES-puro
Plasmid#85144Purposelentiviral expression of human VE-cadherin, C-terminally tagged with mRuby3DepositorInsertCDH5-mRuby3 (VE-cadherin, C-terminally tagged with mRuby3 (CDH5 Human)
UseLentiviralTagsmRuby3ExpressionMutationPromoterCAGAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
ER-mGreenLantern
Plasmid#164463PurposeLocalization and retention of mGreenLantern fluorescent protein in the ERDepositorInsertmGreenLantern
UseTagsCalreticulin and KDELExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEx-4T-1-YTHDF2
Plasmid#52301PurposeEncode YTHDF2 isoform 2 with N-terminal GST tag for E. coli expressionDepositorInsertYTHDF2 (YTHDF2 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-IKZF2-V2-V5
Plasmid#107391PurposeMammalian expression of IKZF2-V2-V5DepositorInsertIKZF2-V2 (IKZF2 Human)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
p426_Cas9_gRNA-ARS208a
Plasmid#87383PurposeAll-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS208a sequence GTCCGCTAAACAAAAGATCT in yeast chromosome 2.DepositorInsertHuman Optimized S. pyogenes Cas9 and guide RNA targeting ARS208a
UseCRISPRTagsExpressionMutationPromoterADH1, pTyrosineAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-CHAMP1
Plasmid#159892PurposeExpresses CHAMP1 (CAMP) in mammalian cellsDepositorInsertCHAMP1 (CHAMP1 Human)
UseTagspEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
SHC003BSD-EXOC7
Plasmid#133298PurposeExpresses EXOC7 in mammalian cellsDepositorInsertExocyst complex component 7 (EXOC7 Human)
UseLentiviralTags3XFLAGExpressionMammalianMutationPromoterCMV promoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
NLS-4LNDD-pcDNA3.1(+)
Plasmid#172313PurposeExpresses ADAR2 Deaminase Domain (DD) fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS) in mammalian cellsDepositorInsertLambda N peptide - human ADAR2 Deaminase Domain
UseTags6xHis, Flag, and Nuclear Localization Signal of S…ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMXS-hs-3xHA-LIN-41
Plasmid#52717Purposeretroviral expression of human LIN-41 / TRIM71 with 3 N-terminal HA tagsDepositorInsertLIN-41 (TRIM71 Human)
UseRetroviralTags3xHAExpressionMammalianMutationPromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 blast tBID-flag
Plasmid#211533PurposeExpresses truncated BID upon doxycycline addition to trigger apoptosisDepositorInserttBID-flag (BID Human)
UseRetroviralTagsFLAGExpressionMammalianMutationTruncated (CASP8) form (p15)PromoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPCX2-HA-GATA3 FL
Plasmid#89835PurposeExpress GATA3 in mammalian cellsDepositorInsertGata3 (Gata3 Mouse)
UseRetroviralTagsHAExpressionMutationPromoterCMVAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFLAG-Tetherin
Plasmid#41070DepositorInsertTetherin (BST2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCIG AKT3 E17K
Plasmid#73050PurposeHuman AKT3 E17KDepositorInsertAKT3 E17K (AKT3 Human)
UseTagsExpressionMammalianMutationE17KPromoterAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-IKZF1-V2-V5
Plasmid#107390PurposeMammalian expression of IKZF1-V2-V5DepositorInsertIKZF1-V2 (IKZF1 Human)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only