164,577 results

1. AAVS1 SA-2A-puro-pA donor

   Plasmid

   #22075

   Purpose

   AAV zinc finger donor vector for genomic targeting

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

   Insert

   SA-Puro
   

   Use

   Zfn donor

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIRE4-Bpa

   Plasmid

   #155342

   Purpose

   Plasmid encoding the translational pair that enables efficient incorporation of Benzoyl-phenylalanine in response to the amber codon into proteins in mammmalian cells.

   Depositor

   Irene Coin

   Article

   Bottke et al EMBO Rep. 2020 Sep 14:e50437. doi: 10.15

   Insert

   humanized EBpaRS/BstYam
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK/U6

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. L4440_BioBrick-SCR-sgRNA-A

   Plasmid

   #177811

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   Scrambled sgRNA targeting sequences
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-BICD2

   Plasmid

   #164626

   Purpose

   Expresses BICD2 in mammalian cells with a GFP tag

   Depositor

   Mark Cookson

   Article

   Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

   Insert

   BICD2 (BICD2 Human)
   

   Use

   Tags

   Cycle 3 GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK5-mEGFP-DVL1-WT

   Plasmid

   #194586

   Purpose

   Mammalian Expression of mEGFP-DVL1 WT Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

   Insert

   DVL1 (DVL1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. FLAG-IKKbeta (S177E,S181E)-pcw107

   Plasmid

   #64609

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   Kris Wood, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   IKBKB
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   S177E, S181E

   Promoter

   PGK

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

7. tetO.Nfib.Hygro

   Plasmid

   #117271

   Purpose

   Expresses Nfib and hygromycin resistance gene under control of tetO sequence.

   Depositor

   Henrik Ahlenius

   Article

   Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi

   Insert

   Nfib (Nfib Mouse)
   

   Use

   Lentiviral

   Tags

   IRES and hygromycin resistant cassette (C termina…

   Expression

   Mammalian

   Mutation

   Promoter

   tet-on

   Available Since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Tet-inducible Luciferase reporter

   Plasmid

   #64127

   Purpose

   Photoactivatable transcription system. Luciferase reporter under the control of a tet-inducible promoter.

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetOx13-CMV

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB-R1R2_EF1aVP64dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

   Plasmid

   #113341

   Purpose

   Expresses VP64-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

   Depositor

   Gavin Wright

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   VP64-dCas9-VP64-T2A-MS2-p65-HSF1
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-MP-84-spCas9-U6-gRNA

    Plasmid

    #206935

    Purpose

    Expression of spCas9 endonuclease and gRNA in an AAV vector allowing packaging of viral genome smaller than 5 kb.

    Depositor

    Markus Grompe

    Insert

    spCas9
    

    Use

    AAV

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    MP-84

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1_ 2xFLAG-2xSTREP_NRF2

    Plasmid

    #159129

    Purpose

    expresses NRF2 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

    Insert

    NRF2 (NFE2L2 Human)
    

    Use

    Tags

    2X-FLAG _ 2X-STREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMK411 (OsTIR1(F74G) mAID-EGFP-Nluc)

    Plasmid

    #140659

    Purpose

    OsTIR1(F74G)-P2A-mAID-EGFP-Nluc

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    OsTIR1(F74G) mAID-EGFP-Nluc TOL2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Myosin-IIA-GFP

    Plasmid

    #38297

    Depositor

    Matthew Krummel

    Article

    Jacobelli et al J Immunol. 2009 Feb 15;182(4):2041-50.

    Insert

    Myosin II A (Myh9 Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3 IKKE K38A flag

    Plasmid

    #26202

    Depositor

    Tom Maniatis

    Article

    Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

    Insert

    I-kappa-B kinase epsilon K38A (IKBKE Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Catalytic Lysine changed to Alanine (K38A)

    Promoter

    Available Since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAGGS_VP35_EBOV

    Plasmid

    #103050

    Purpose

    Encodes Ebola virus VP35 gene (Zaire ebolavirus, Mayinga) under control of the CAG promoter

    Depositor

    Elke Mühlberger

    Article

    Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

    Insert

    VP35 gene of Ebola virus (VP35 (Zaire ebolavirus, Mayinga))
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-PS11-scFvFc-CD28-gp41 (706-713)

    Plasmid

    #60606

    Purpose

    Mammalian Display vector

    Depositor

    Wayne Marasco

    Article

    Taube et al PLoS One. 2008 Sep 11;3(9):e3181. doi: 1

    Insert

    PS11-scFvFc-CD28-gp41 (706-713) (CD28 Human)
    

    Use

    Tags

    C9

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

17. JME4580

    Plasmid

    #129659

    Purpose

    HPHex_CrisprCas9-yl_RFP: CAS9 vector with Hygromycinex marker for gRNA cloning using GoldenGate

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG:GPI-GFP

    Plasmid

    #32601

    Depositor

    Anna-Katerina Hadjantonakis

    Article

    Rhee et al Genesis. 2006 Apr;44(4):202-18.

    Insert

    GPI-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    March 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. SpCas9-NG-ABE8e(V106W)

    Plasmid

    #198533

    Purpose

    Expresses human codon-optimized SpCas9-NG-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-NG-ABE8e(V106W)-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NG-ABE8e(V106W)
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. ND6-DdCBE-left side TALE

    Plasmid

    #157842

    Purpose

    expresses left-side TALE–DddAtox half of ND6-DdCBE in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    SOD2 MTS–3x-HA–ND6 left tale–G1397 DddA-N–UGI–SOD2 3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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