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8,969 results

Showing: 7081 - 7110 of 8969 results

  2. pRubiG-T2A-Cas9

    Plasmid
    #75348
    Purpose
    Ubiquitin promoter expresses GFP and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.
    Depositor
    Bryan Luikart
    Article
    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10
    Insert
    Cas9
    Use
    CRISPR and Retroviral
    Tags
    GFP (via t2a)
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    May 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    75348-plasmid-map-sequence-id-142640

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  6. ChR2-2A-Halo

    Plasmid
    #22047
    Purpose
    Expression of ChR2-GFP and Halo-YFP for bi-directional control of neuronal activity
    Depositor
    Edward Boyden
    Article
    Han et al Front Mol Neurosci. 2009 Aug 27:2:12. do
    Insert
    ChR2-GFP-2A-Halo-YFP
    Tags
    GFP and YFP
    Expression
    Mammalian
    Mutation
    Note that there is a S147R mut in YFP. This mutat…
    Promoter
    CMV
    Available Since
    Sept. 17, 2009
    Availability
    Academic Institutions and Nonprofits only
    22047-plasmid-map-sequence-id-263449

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  7. pAAV-EF1a-N-CretrcintG

    Plasmid
    #69570
    Purpose
    One half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for rAAV production and expression in mammalian cells
    Depositor
    Connie Cepko
    Article
    Tang et al Nat Neurosci. 2015 Sep;18(9):1334-41. do
    Has Service
    AAV1
    Insert
    kozak consensus - N-CretrcintG
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Oct. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    69570-plasmid-map-sequence-id-127742

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  8. pAAV.synP.DIO.EGFP.WPRE.hGH

    Plasmid
    #100043
    Purpose
    An AAV vector that expresses EGFP in a Cre-dependent manner (DIO, double floxed inverted open reading frame).
    Depositor
    Ian Wickersham
    Has Service
    AAV9
    Insert
    EGFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    synapsin-1
    Available Since
    Jan. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    100043-plasmid-map-sequence-id-193613

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  16. pSBbi-GN

    Plasmid
    #60517
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and neomycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60517-plasmid-map-sequence-id-379976

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  17. CMV-G-GECO1.1

    Plasmid
    #32445
    Purpose
    Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.1)
    Depositor
    Robert Campbell
    Article
    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.
    Insert
    G-GECO1.1
    Expression
    Mammalian
    Mutation
    GCaMP3 K69E/N77Y/D86G/K119I/L173Q/K380N/S404G/E43…
    Promoter
    CMV
    Available Since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only
    32445-plasmid-map-sequence-id-150291

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  24. pENTR-GFP-MDC1 (770-7)

    Plasmid
    #26284
    Depositor
    Eric Campeau
    Insert
    Mediator of DNA damage checkpoint 1 (MDC1 Human)
    Use
    Gateway Cloning
    Tags
    GFP
    Available Since
    Oct. 18, 2010
    Availability
    Academic Institutions and Nonprofits only
    26284-plasmid-map-sequence-id-12370

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Showing: 7081 - 7110 of 8969 results