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Showing: 9521 - 9560 of 157662 results

  2. PL-SIN-EOS-C(3+)-EiP

    Plasmid
    #21313
    Purpose
    Lentiviral EOS reporter with Oct3/4 enhancer (x3), expresses EGFP and Puro resistance
    Depositor
    James Ellis
    Article
    Hotta et al Nat Methods. 2009 May . 6(5):370-6.
    Insert
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EOS-C(3+) promoter region
    Available since
    June 1, 2009
    Availability
    Academic Institutions and Nonprofits only
    21313-plasmid-map-sequence-id-9854

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  3. mEmerald-CLIP170-N-18

    Plasmid
    #54044
    Purpose
    Localization: Microtubule Binding , Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    CLIP170 (CLIP1 Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54044-plasmid-map-sequence-id-139284

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  4. TDP-REGv2 Cryptic PE-Max

    Plasmid
    #216163
    Purpose
    Expression of prime editor PEMax (Plasmid #174820) only in cells with TDP-43 loss of function. Note: It is not recommended to produce lentivirus containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    PEMax for prime editing with internal cryptic exon
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216163-plasmid-map-sequence-id-437751

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  8. VIPR2-Tango

    Plasmid
    #66521
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    VIPR2 (VIPR2 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. pPD49_83

    Plasmid
    #1448
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    hsp16-25', hsp16-415', IVSA, rf2128
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1448-plasmid-map-sequence-id-59

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  15. Go1-CASE

    Plasmid
    #168123
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Go1. Composed of the subunits G alpha o1 (GNAO1) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAO1, GNB3, GNG9 (GNAO1, GNB3, GNGT2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG9
    Expression
    Mammalian
    Mutation
    NLuc is inserted at L91/G92 within GNAO1
    Promoter
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168123-plasmid-map-sequence-id-332865

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  17. pYFP

    Plasmid
    #175592
    Purpose
    Tet inducible, KAN Resistant, mVenus gene on a low copy backbone. Used as a negative control to the pRelA_YFP plasmid.
    Depositor
    Greg Bokinsky
    Article
    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.
    Insert
    mVenus
    Use
    Tags
    Expression
    Bacterial
    Mutation
    An improved monomeric YFP. See doi: 10.1021/bi051…
    Promoter
    Available since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    175592-plasmid-map-sequence-id-348320

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  22. LO601: pMVP (R4-R3) destabilized firefly luciferase (Luc2P)

    Plasmid
    #132926
    Purpose
    pMVP R4-R3 entry plasmid, contains destabilized Firefly Luciferase (Luc2P) for 3- or 4-component MultiSite Gateway Pro assembly
    Depositor
    Christopher Newgard
    Insert
    Luc2P
    Use
    Luciferase and Synthetic Biology; Pmvp gateway en…
    Tags
    PEST destabilization domain
    Expression
    Mutation
    Promoter
    Available since
    Oct. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    132926-plasmid-map-sequence-id-314396

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  24. pmCherry-C1 R62D actin-3XNLS P2A mCherry

    Plasmid
    #58477
    Purpose
    Expresses nuclear-targeted non-polymerizing R62D mutant of human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter
    Depositor
    Dyche Mullins
    Article
    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife
    Inserts
    beta actin (ACTB Human)
    3XNLS
    P2A cleavage site
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Changed Arginine 62 to Aspartic Acid
    Promoter
    CMV
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    58477-plasmid-map-sequence-id-90386

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  25. TFORF0639

    Plasmid
    #141649
    Purpose
    Lentiviral vector for overexpressing the HOXC6 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    HOXC6 (HOXC6 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141649-plasmid-map-sequence-id-391539

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  29. pTP1122

    Plasmid
    #104159
    Purpose
    Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (3x Cysteines)
    Depositor
    Dirk Görlich
    Article
    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154
    Insert
    Anti-mouse IgG1 Fc specific nanobody TP1107 (3xCysteines)
    Use
    Tags
    14xHistidine tag and NEDD8 from Brachypodium dist…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    104159-plasmid-map-sequence-id-252709

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  31. pCMV-tomm20-ECFP-RspANFAST

    Plasmid
    #216348
    Purpose
    targets ECFP-RspANFAST to mitochondrial membrane
    Depositor
    Alison Tebo
    Insert
    tomm20-ECFP-RspANFAST
    Use
    Tags
    tomm20
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216348-plasmid-map-sequence-id-427395

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  34. pCMV-Popt

    Plasmid
    #231705
    Purpose
    Mammalian expression vector encoding a codon-optimized P gene from vesicular stomatitis virus, strain Indiana.
    Depositor
    Ron Geller
    Article
    Marques et al Sci Rep. 2024 Jun 25;14(1):14644. doi: 1
    Insert
    VSV P gene (codon-optimized )
    Use
    Vsv expression
    Tags
    Expression
    Mammalian
    Mutation
    human codon-optimized
    Promoter
    CMV
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231705-plasmid-map-sequence-id-459827

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  35. pCMV-Lopt

    Plasmid
    #231706
    Purpose
    Mammalian expression vector encoding a codon-optimized L gene from vesicular stomatitis virus, strain Indiana.
    Depositor
    Ron Geller
    Article
    Marques et al Sci Rep. 2024 Jun 25;14(1):14644. doi: 1
    Insert
    VSV L gene (codon-optimized )
    Use
    Vsv expression
    Tags
    Expression
    Mammalian
    Mutation
    human codon-optimized
    Promoter
    CMV
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231706-plasmid-map-sequence-id-459825

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  39. TFORF1829

    Plasmid
    #144195
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    DMRTA2 (DMRTA2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    May 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144195-plasmid-map-sequence-id-392330

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  40. pAAV-synp-F-synaptophysin-GCaMP6f

    Plasmid
    #102996
    Purpose
    Expresses synaptophysin fused GCaMP6f under the synapsin promoter
    Depositor
    David Cox
    Insert
    Synaptophysin-GCaMP6f
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    102996-plasmid-map-sequence-id-218069

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Showing: 9521 - 9560 of 157662 results