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Showing: 921 - 960 of 70673 results

  3. Anti-ADAM22-cytoplasmic [N46/30-2b] - Chimeric

    Plasmid
    #227027
    Purpose
    Mammalian expression plasmid of anti-ADAM22-cytoplasmic (Mouse). Derived from hybridoma N46/30.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Anti-ADAM22-cytoplasmic (Mus musculus) recombinant mouse monoclonal antibody. (Adam22 Mouse)
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    227027-plasmid-map-sequence-id-452417

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  4. qTAG-C-mStayGold-EFS-Blast-ARL13B

    Plasmid
    #227278
    Purpose
    Donor template for mStayGold-EFS-Blast insertion into the C-terminus of the ARL13B locus. For cilia visualization. To be co-transfected with plasmid pX330-PITCh-ARL13B (Addgene #227276)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    ARL13B Homology Arms flanking a mStayGold-EFS-Blast Cassette (ARL13B Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227278-plasmid-map-sequence-id-451732

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  13. pGMC00024 (aka pCW-Cas9n-Blast)

    Plasmid
    #195315
    Purpose
    Dox inducible lentiviral Cas9 nickase
    Depositor
    Raj Chari
    Insert
    Cas9-nickase
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A nickase
    Promoter
    Available since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    195315-plasmid-map-sequence-id-390525

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  14. lenti-Cas9-Exo-Blast-BFP

    Plasmid
    #196721
    Purpose
    Sortable and selectable SpCas9 fused to exodeoxyribonuclease I (sbcB) from E. coli. For the creation of longer deletions.
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-Exo1-T2A-BSD-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196721-plasmid-map-sequence-id-389753

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  19. fru-T2A-QF2 HDR plasmid

    Plasmid
    #141099
    Purpose
    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti fru gene
    Depositor
    Leslie Vosshall
    Article
    Basrur et al Elife. 2020 Dec 7;9. pii: 63982. doi: 10
    Insert
    fru-left-HDR-arm; T2A-QF2-hsp70; 3xP3-dsRed-SV40; fru-right-HDR-arm
    Use
    Donor template
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    141099-plasmid-map-sequence-id-292289

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  20. pLenti-CMV-GFP-miRE_shTHUMPD3-Blast

    Plasmid
    #135690
    Purpose
    Constitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded shROSA26 (control shRNA), Blasticidin selection
    Depositor
    Florian Karreth
    Insert
    shRosa26
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135690-plasmid-map-sequence-id-327708

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  21. pLenti-CMV-GFP-miRE_shSCR-Blast

    Plasmid
    #135688
    Purpose
    Constitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded scrambled control shRNA, Blasticidin selection
    Depositor
    Florian Karreth
    Insert
    shScrambled
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135688-plasmid-map-sequence-id-327712

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  22. pBlastR-MCS-LexA-VP16-MiniWhite

    Plasmid
    #165900
    Purpose
    Blasticidin resistant LexA driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloning
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Insert
    Selectable Empty Enhancer Driver
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    165900-plasmid-map-sequence-id-326306

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  23. pMXS-IRES-Blast HA-GPAT4-ΔNs5

    Plasmid
    #173169
    Purpose
    Retroviral vector to express sgRNA resistant HA tagged human GPAT4 with mutation in CHP1 binding site
    Depositor
    Kivanc Birsoy
    Article
    Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi
    Insert
    Glycerol-3-Phosphate Acyltransferase 4 (GPAT4 Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mutation
    Synonymous mutations at sgRNA sites, amino acids …
    Promoter
    MMLV
    Available since
    Aug. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    173169-plasmid-map-sequence-id-341378

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  24. pCRISPaint-mScarlet-F-Ova-BlastR [M1G]

    Plasmid
    #171817
    Purpose
    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and blasticidin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mScarlet-F-Ova-T2A-BlastR
    Use
    Gene tagging
    Tags
    Expression
    Mutation
    BlastR: Changed Methionin 1 to Glycine.
    Promoter
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    171817-plasmid-map-sequence-id-338126

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  25. pCRISPaint-mScarlet-F-hgp100-BlastR [M1G]

    Plasmid
    #171815
    Purpose
    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and blasticidin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mScarlet-F-hgp100-T2A-BlastR
    Use
    Gene tagging
    Tags
    Expression
    Mutation
    BlastR: Changed Methionin 1 to Glycine.
    Promoter
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    171815-plasmid-map-sequence-id-338123

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  27. pCRISPaint-mNeon-F-Ova-BlastR [M1G]

    Plasmid
    #171808
    Purpose
    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and blasticidin resistance cassette [p.M1G
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mNeonGreen-F-Ova-T2A-BlastR
    Use
    Gene tagging
    Tags
    Expression
    Mutation
    BlastR: Changed Methionin 1 to Glycine.
    Promoter
    Available since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171808-plasmid-map-sequence-id-337980

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  28. pCRISPaint-mNeon-F-hgp100-BlastR [M1G]

    Plasmid
    #171806
    Purpose
    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and blasticidin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mNeonGreen-F-hgp100-T2A-BlastR
    Use
    Gene tagging
    Tags
    Expression
    Mutation
    BlastR: Changed Methionin 1 to Glycine.
    Promoter
    Available since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171806-plasmid-map-sequence-id-337977

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  30. Equalizer-M plasmid with onboard mCherry cassette

    Plasmid
    #169734
    Purpose
    Equalizer-M plasmid that encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.
    Depositor
    Francois St-Pierre
    Article
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Insert
    tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-tetO2
    Available since
    June 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    169734-plasmid-map-sequence-id-334141

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  31. Equalizer-L plasmid with onboard mCherry cassette

    Plasmid
    #169735
    Purpose
    Equalizer-L plasmid that encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.
    Depositor
    Francois St-Pierre
    Article
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Insert
    miR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-tetO2
    Available since
    June 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    169735-plasmid-map-sequence-id-334142

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  32. pMY-CD90.2-P2A-BlastDYK-Rluc_miR

    Plasmid
    #163341
    Purpose
    Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface marker and a DYK-tagged Blasticidin selection marker
    Depositor
    Louis Ates, Teunis Geijtenbeek
    Article
    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.
    Insert
    CD90.2 (Thy1 Mouse)
    Use
    RNAi and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    LTR
    Available since
    Jan. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    163341-plasmid-map-sequence-id-322055

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  34. Ir7f-T2A-QF2 HDR plasmid

    Plasmid
    #140942
    Purpose
    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Ir7f gene
    Depositor
    Leslie Vosshall
    Article
    Jove et al Neuron. 2020 Dec 23;108(6):1163-1180.e12
    Insert
    Ir7f-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir7f-right-HDR-arm
    Use
    Tags
    Expression
    Mutation
    4 point mutations (annotated on plasmid map) were…
    Promoter
    Available since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    140942-plasmid-map-sequence-id-313600

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  35. Ir7a-T2A-QF2 HDR plasmid

    Plasmid
    #140943
    Purpose
    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Ir7a gene
    Depositor
    Leslie Vosshall
    Article
    Jove et al Neuron. 2020 Dec 23;108(6):1163-1180.e12
    Insert
    Ir7a-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir7a-right-HDR-arm
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    140943-plasmid-map-sequence-id-313597

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  36. KZ301: pMVP (L3-L2) P2A-Blast + polyA

    Plasmid
    #121779
    Purpose
    pMVP L3-L2 entry plasmid, contains Blast-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Blast selection marker linked by P2A to gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    P2A-Blast + polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121779-plasmid-map-sequence-id-237275

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  37. LA401: pMVP (L3-L2) P2A-Blast + WPRE

    Plasmid
    #121784
    Purpose
    pMVP L3-L2 entry plasmid, contains Blast-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Blast selection marker linked by P2A to gene in lentivirus vectors
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    P2A-Blast + WPRE
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121784-plasmid-map-sequence-id-237282

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  38. IH101: pMVP (L5-L4) OLLAS epitope tag

    Plasmid
    #121703
    Purpose
    pMVP L5-L4 entry plasmid, contains OLLAS epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    OLLAS epitope tag (open)
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121703-plasmid-map-sequence-id-237109

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Showing: 921 - 960 of 70673 results