We narrowed to 24,265 results for: nes
-
Plasmid#170124PurposeLentiviral vector carrying the MCP-ADAR2 with a nuclear export signalDepositorInsertMCP-ADAR2-DD-NES
UseLentiviralTagsExpressionMammalianMutationPromoterEf1a core promoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQP-NiRISB
Plasmid#138977PurposeLight-controlled tri-directional targeting of endogenous proteins (nucleus-shifted)DepositorInsertNcoI-BphP1-CAAX-IRES2-NES- iB(GFP)- mCherry-Q-PAS1-AsLOV2cNLS-NotI
UseTagsExpressionMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV0479
Plasmid#133499PurposeExpression of sfGFP excluded from the nucleus with two NES signalsDepositorInsertpHis5(StuI)-p(tdh1)-NESwis1-sfGFP-NESmia1-natMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
Split-RESCUE-C
Plasmid#170154PurposeAAV vector carrying the RESCUE-DDC-λN (RESCUE is the evolved ADAR2 variant for C-U editing) with a nuclear export signalDepositorInsertRESCUE-DDC-λN-NES
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQP-iRISB
Plasmid#138979PurposeLight-controlled tri-directional targeting of endogenous proteinsDepositorInsertNcoI-BphP1-CAAX-IRES2-NES-SGlinkeriB( GFP)-mCherry-Q-PAS1-AsLOV2cNLS-NotI
UseTagsExpressionMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
-
LAMP1-APEX2
Plasmid#215555PurposeStable, inducible expression in lysosomeDepositorInsertLAMP1-EGFP-Flag tag-APEX2-NES
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cyto-APEX2
Plasmid#215552PurposeStable, inducible expression in cytoplasmDepositorInsertEGFP-Flag tag-APEX2-NES
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
MCP-ADAR2-DD(E488Q)
Plasmid#170125PurposeLentiviral vector carrying the MCP-ADAR2(E488Q) with a nuclear export signalDepositorInsertMCP-ADAR2-DD(E488Q)-NES
UseLentiviralTagsExpressionMammalianMutationPromoterEf1a core promoterAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
SLF063: Lenti-fCAG-jRGECO1a
Plasmid#124775PurposeFor general lentiviral or direct expression of cytosolic jRGECO1aDepositorInsertjRGECO1a
UseLentiviralTagsNES-6xHISExpressionMammalianMutationPromoterCAGAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
FKBP-NTEVp(WT)
Plasmid#137831PurposeCytosolic split-TEV protease chain with FKBP Rapamycin sensing domain, N-terminal WT TEV proteaseDepositorInsertFKBP-NTEVp(WT)
UseTags3x FLAG and NESExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
FRB-CTEVp(WT)
Plasmid#137832PurposeCytosolic split-TEV protease chain with FRB Rapamycin sensing domain, C-terminal WT TEV proteaseDepositorInsertFRB-CTEVp(WT)
UseTags3x FLAG and NESExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-cytoEKAR4
Plasmid#174438PurposeCytosol-targeted EKAR4.DepositorInsertcytoEKAR4
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cyto-ABKAR
Plasmid#61510PurposeExpression of AMPK biosensorDepositorInsertAMPK Biosensor
UseTagsNESExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
Endo-APEX2
Plasmid#215553PurposeStable, inducible expression in endosomeDepositorInsertEGFP-2xFYVE-APEX2-NES
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
GALT-APEX2
Plasmid#215554PurposeStable, inducible expression in GolgiDepositorInsertAPEX2-NES-GalT-EGFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQP-2460
Plasmid#138973PurposeLight-induced recruitment of QPAS1-mCherry to the target protein bound to membrane; interaction occurs via intrabody fused to BphP1DepositorInsertNcoI-mVenus-CAAX-IRES2-BphP1- iB(GFP)- IRES2-NES-mCherry-Q-PAS1-NotI
UseTagsExpressionMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
MCP-ADAR2-DD(E488Q, N496F)
Plasmid#170139PurposeAAV vector carrying the MCP-ADAR2-DD(E488Q, N496F) with a nuclear export signalDepositorInsertNES-MCP-ADAR2-DD(E488Q, N496F)
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Split-RESCUE-N
Plasmid#170153PurposeAAV vector carrying the MCP-RESCUE-DDN (RESCUE is the evolved ADAR2 variant for C-U editing) with a nuclear export signalDepositorInsertNES-MCP-RESCUE-DDN
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAGM16467
Plasmid#105403PurposeSynthetic biologyDepositorInsert6xHis-NES(PKI)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
LAMTOR1-APEX2
Plasmid#215556PurposeStable, inducible expression in lysosomeDepositorInsertLAMTOR1-EGFP-Flag tag-APEX2-NES
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQP-2363
Plasmid#138975PurposeRelocalization of genomically expressed protein of interest from cytoplasm to nucleus upon near-infrared illuminationDepositorInsertNcoI-BphP1- iB(GFP) -IRES2-NES-mCherry-QPAS1- NLS-NotI
UseTagsExpressionMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAGM16418
Plasmid#105404PurposeSynthetic biologyDepositorInsert6xHis-NES(Rev)-GFP
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAGM16429
Plasmid#105405PurposeSynthetic biologyDepositorInsert6xHis-NES(PKI)-GFP
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAGM16456
Plasmid#105402PurposeSynthetic biologyDepositorInsert6xHis-NES(Rev)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
PM-APEX2
Plasmid#215557PurposeStable, inducible expression in plasma membraneDepositorInsertLyn11-EGFP-Flag tag-APEX2-NES
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-NEW-fRGECO2
Plasmid#125248PurposeExpresses red fluorescent calcium sensor in mammalian cellsDepositorInsertfRGECO2
UseTagsNES signalExpressionMammalianMutationPromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV Syn CaMPARI2-human-a-Tubulin (TubuTag)
Plasmid#164184PurposeFreeze-frame imaging of dendritic calcium signals CaMPARI2 moiety with lower Ca+ affinity (Kd=199nM) (Moeyaert et al., 2018)DepositorInsertsCamPARI2
Human α Tubulin
UseAAVTags6 His tag and NESExpressionMammalianMutationPromoterSynAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJJiaDEST-APEX2
Plasmid#149517PurposeN-termial APEX2 tag. Gateway destination vector for mammanlian expressionDepositorTypeEmpty backboneUseTagsFLAG-APEX2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
-
pDV24_AAV2_DART-VADAR_iRFP720-sensor
Plasmid#200411PurposeAAV vector encoding a DART-VADAR sensor for iRFP720 mRNADepositorInsertDART VADAR sensor
UseAAV and Synthetic BiologyTagsMCP-ADAR2dd(E488Q)-NES, TagBFP, and mNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDN80
Plasmid#61349PurposeLINuS-flex: modular LINuS construct enabling introduction of any protein encoding sequence, NES and NLS. The sequence of interest replaces the LexA DBD-MBP-VP64 fragment.DepositorTypeEmpty backboneUseTagsAsLOV2 wt and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
SLF060: Lenti-CaMKIIa-jRGECO1a
Plasmid#112282PurposeFor lentiviral or direct expression of cytosolic jRGECO1a in excitatory neuronsDepositorInsertjRGECO1a
UseLentiviralTagsNES-6xHISExpressionMammalianMutationPromoterCaMKIaAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK4a_002
Plasmid#123816PurposeEncodes Nuclear Export sequence as a Type 4a part to be used in the MTK systemDepositorInsertNES
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK3a_002
Plasmid#123755PurposeEncodes Nuclear Export sequence as a Type 3a part to be used in the MTK systemDepositorInsertNES
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKG3666_pGEEC576_pShip-EFS-ZF43a(inducible)-P2A-iRFP720-bGH
Plasmid#239740PurposePlasmid expressing a rapamycin-inducible transcriptional activatorDepositorInsertNLS-FXBP-ZF43-P2A-NES-VP64-FRB-P2A-iRFP720
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
nCaMK2rep
Plasmid#239624PurposeCaMKII activity reporter. The lentiviral plasmid contains a nanobody sequence (PSD95.FingR), 3xmyc tags and double phospho-site 3 from rat Synapsin-1a.DepositorInsertPSD95.FingR-NES-SynP3-3xmyc (Syn1 Rat, Synthetic)
UseCre/Lox and LentiviralTagsFlag and mycExpressionMammalianMutationPromoterhSynAvailable sinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pQP-2376
Plasmid#138974PurposeLight-induced recruitment of NSImb-QPAS1-mCherry to the target protein bound to membrane; interaction occurs via intrabody fused to BphP1DepositorInsertNcoI-mVenus-CAAX-IRES2-BphP1- iB(GFP)- IRES2-NSImb-NES-mCh-Q-PAS1-NotI
UseTagsExpressionMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PKAsub-YPet-Kras
Plasmid#138206PurposeEncodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (bimAKAR); plasma membrane targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.DepositorInsertPKAsub-YPet-Kras
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAAV Syn CaMPARI2-F391W_L398V-human-a-Tubulin (high affinity TubuTag)
Plasmid#164193PurposeFreeze-frame imaging of dendritic calcium signals High calcium affinity (CaMPARI2_F391W_L398V moiety Kd=174nM)DepositorInsertsCamPARI2
Human α Tubulin
UseAAVTags6 His tag and NESExpressionMammalianMutationF391W-L398VPromoterSynAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
pEarleyGate 100RMOA mPing
Plasmid#196137PurposeMeasures mPing transposition in plantsDepositorInsertsPong TPase LA T2A ORF1SC1 ONE
mPing
UseBinaryTagsExpressionPlantMutationTPase NES sequence mutated L418A, L420A; T2A pept…Promoter35S and RPS5aAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
Pvha6-AgDD
Plasmid#80626Purposecreate protein aggregates in C. elegansDepositorInsertNES-L106P-sfGFP (FKBP1A Human)
UseTagsMLALKLAGLDIExpressionWormMutationPromoterAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
Bxb1_jRCaMP1b
Plasmid#183758PurposeBxb1-specific donor plasmid for intergrating a transcription unit for expression of jRCaMP1b calcium reporterDepositorInsertjRCaMP1b
UseTagsNESExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AMPKsub-YPet-Kras
Plasmid#84638PurposeEncodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertAMPKsub-YPet-Kras
UseTags6xHis, C-terminal targeting sequence from Kras, T…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
nCaMK2rep-S603A
Plasmid#239625PurposeInactive CaMKII activity reporter. The lentiviral plasmid contains a nanobody sequence (PSD95.FingR), 3xmyc tags and double mutated phospho-site 3 from rat Synapsin-1a.DepositorInsertPSD95.FingR-NES-SynP3-3xmyc (Syn1 Rat, Synthetic)
UseCre/Lox and LentiviralTagsFlag and mycExpressionMammalianMutationChanged serine 603 to AlaninePromoterhSynAvailable sinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
ARB344
Plasmid#124027PurposeEncodes TU with connectors L1 R2 and an B1 insulator, and pCMV expressing cytoplasmic iRFP713DepositorInsertL1-B1_CMV-NES::iRFP713-rglPA_R2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
ARB345
Plasmid#124028PurposeEncodes TU with connectors L1 R2 and an B1 insulator, and pCMV expressing cytoplasmic tagBFPDepositorInsertL1-B1_CMV-NES::tagBFP-rglPA_R2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
ARB346
Plasmid#124029PurposeEncodes TU with connectors L1 R2 and an B1 insulator, and pCMV expressing cytoplasmic mAzamiGreenDepositorInsertL1-B1_CMV-NES::mAzamiGreen-rglPA_R2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
ARB347
Plasmid#124030PurposeEncodes TU with connectors L1 R2 and an B1 insulator, and pCMV expressing cytoplasmic mRuby2DepositorInsertL1-B1_CMV-NES::mRuby2-rglPA_R2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only