We narrowed to 15,962 results for: GRN

1. L1_lacZgRNA-Ck2

   Plasmid

   #136137

   Purpose

   L1 in position 2, to clone gRNA target using BbsI, lacZ blue-white screening

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-MpU6:lacZgRNA
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pgRNAtet-lvaA

   Plasmid

   #106397

   Purpose

   Guide RNA plasmid targeting lvaA on BBR1-UP origin

   Depositor

   Brian Pfleger

   Article

   Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

   Insert

   sgRNA towards lvaA in Pseudomonas putida
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   March 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. ATG16L1 sgRNA

   Plasmid

   #207563

   Purpose

   pX330 expressing Cas9 and a sgRNA targeting the ATG16L1 locus

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   GCAGCAAGTGACATGTCGTC
   

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. U6-sgRNA-GFP

   Plasmid

   #200068

   Purpose

   For the knock down of GFP with an astrocyte specific mCherry expression

   Depositor

   Baljit Khakh

   Article

   Ollivier et al Nature. 2024 Mar;627(8003):358-366. doi:

   Insert

   U6-sgRNA-GFP
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   GfaABC1D-mCherry

   Promoter

   U6, GfaABC1D

   Available Since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO5.sgRNA.EFS.tRFP657

   Plasmid

   #57824

   Purpose

   Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP657, EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   sgRNA
   EFS
   tagRFP657
   

   Use

   CRISPR and Lentiviral

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDestTol2pA2-U6:gRNA

   Plasmid

   #63157

   Purpose

   Destination vector for Gateway based on vector #394 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter.

   Depositor

   Leonard Zon

   Article

   Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

   Type

   Empty backbone

   Use

   CRISPR; Tol2 destination vector for gateway

   Available Since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUltra-U6-gRNAs1-5

   Plasmid

   #178569

   Purpose

   Constitutive expression of a gRNA-tRNA array for macsGESTALT editing

   Depositor

   Christopher Lengner

   Article

   Simeonov et al Cancer Cell. 2021 Aug 9;39(8):1150-1162.

   Insert

   5 gRNA-tRNA array with U6 promoter
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX-sgRNA

   Plasmid

   #50662

   Purpose

   Lentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)

   Depositor

   Eric Lander, David Sabatini

   Article

   Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

   Insert

   sgAAVS1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKDsgRNA-pgaC

   Plasmid

   #154919

   Purpose

   aTc inducible gRNA targeting pgaC along with arabinose-inducible lambda red enzymes

   Depositor

   Michael Lynch

   Article

   Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

   Insert

   gRNA targeting pgaC
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   pTet

   Available Since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLH-sgRNA1

    Plasmid

    #75388

    Purpose

    sgRNA1

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    sgRNA1
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. LentiCRISPRv2 NT sgRNA3

    Plasmid

    #164929

    Purpose

    Expresses Non-targeting sgRNA3 control in mammalian cells

    Depositor

    Ji Luo

    Article

    Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

    Insert

    Non-targeting sgRNA3 (verified for knockout)
    

    Use

    Lentiviral

    Promoter

    EFS promoter

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6_HEK3_nsgRNA(PP7)

    Plasmid

    #232444

    Purpose

    nsgRNA for PE3 facilitation of a +1 T to A prime edit on the HEK3 locus, contains a PP7 in the scaffold tetraloop

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    PP7-tagged nicking gRNA (PE3) for HEK3+1t>a edit driven by human U6 promoter
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDT-sgRNA

    Plasmid

    #138271

    Purpose

    Dual-targeting sgRNA vector that contains both a sgRNA against BFP (sgBG) and a sgRNA for a genomic target site (sgTS)

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

    Insert

    sgBG, sgTS
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. SOX17-3'gRNA

    Plasmid

    #210465

    Purpose

    gRNA targeting SOX17 3' terminal for CRISPR-Cas9-mediated knock-in

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Insert

    SOX17 (SOX17 Human)
    

    Use

    CRISPR

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-gRNA-Puro

    Plasmid

    #121121

    Purpose

    Expression of a gRNA together with a Puromycin resistance

    Depositor

    Pablo Navarro

    Article

    Heurtier et al Nat Commun. 2019 Mar 7;10(1):1109. doi:

    Insert

    gRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGGDestTol2LC-5sgRNA

    Plasmid

    #64243

    Purpose

    destination vector for five U6x:sgRNA cassettes

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    May 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pgRNA-modified

    Plasmid

    #84477

    Purpose

    guide RNA expression lentiviral construct

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

    Insert

    guide RNA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDNR-gRNA

    Plasmid

    #206990

    Purpose

    A plasmid for expression of SaCas9-sgRNA targeting donor plasmids

    Depositor

    Alejandro Chavez

    Article

    Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

    Insert

    Donor plasmid-targeting SaCas9-gRNA
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCF142_U6-sgRNA

    Plasmid

    #225960

    Purpose

    U6-sgRNA (recipient). Expresses U6-sgRNA (recipient) for VLP production. This is a recipient vector for cloning of specific SpyCas9 sgRNAs.

    Depositor

    Christof Fellmann

    Article

    Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

    Insert

    U6-sgRNA (recipient)
    

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC57kan-T7-gRNA-U6 V2

    Plasmid

    #115520

    Purpose

    To construct multiple sgRNA expression vector for CRISPR

    Depositor

    Xingxu Huang

    Insert

    sgRNA scaffolf-U6 promoter
    

    Use

    CRISPR

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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