We narrowed to 10,664 results for: t7

1. SpyTag003-sfGFP

   Plasmid

   #133454

   Purpose

   Expresses SpyTag003-superfolder GFP in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyTag003-sfGFP
   

   Tags

   GSGSGS linker and His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMBP-AaCas12b

   Plasmid

   #113433

   Purpose

   Expresses Expresses 10xHis-MBP fused to AaCas12b

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Insert

   AaCas12b
   

   Tags

   MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDMyc-TNPO1

   Plasmid

   #86081

   Purpose

   A mammalian expression plasmid encoding Transportin-1 (TNPO1) with N-terminus double Myc-tag.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   Transportin-1 (TNPO1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-15b-EK_C122S_His5

   Plasmid

   #49048

   Purpose

   Expresses the pentahistidine-tagged catalytic domain of bovine enteropeptidase in E. coli

   Depositor

   Hans Brandstetter

   Article

   Skala et al J Biotechnol. 2013 Dec;168(4):421-5. doi

   Insert

   Enteropeptidase, peptidase domain (TMPRSS15 Bovine)
   

   Tags

   5xHis

   Expression

   Bacterial

   Mutation

   P92R, E185D, C122S

   Promoter

   T7

   Available Since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET15b-17X-PTDH

   Plasmid

   #166786

   Purpose

   Phosphite dehydrogenase for NAD(P)H regeneration

   Depositor

   Wilfred van der Donk

   Article

   Yang et al J Am Chem Soc. 2015 Oct 7;137(39):12426-

   Insert

   Phosphite dehydroenase
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   A196R, T201S, A328T, E352N, and C356D- please see…

   Promoter

   T7

   Available Since

   May 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-MBP-mSA2

   Plasmid

   #52319

   Purpose

   Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)

   Depositor

   Sheldon Park

   Article

   Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6

   Insert

   monomeric streptavidin 2
   

   Tags

   FLAG, His, MBP, and TEV

   Expression

   Bacterial

   Mutation

   S25H mutation compared to streptavidin

   Promoter

   T7

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-Flag-Kdm4b-pA

   Plasmid

   #104486

   Purpose

   Express mouse Kdm4b in mammalian cells. It has a T7 promoter for in vitro transcription. Since polyA is encoded on the vector, polyA-tailing is unnecessary.

   Depositor

   Yi Zhang

   Article

   Inoue et al Genes Dev. 2017 Oct 1;31(19):1927-1932.

   Insert

   Kdm4b (Kdm4b Mouse)
   

   Tags

   Flag epitope tag

   Expression

   Mammalian

   Available Since

   Dec. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMBP-FnCas12a

   Plasmid

   #113432

   Purpose

   Expresses 10xHis-MBP fused to FnCas12a

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Insert

   FnCas12a
   

   Tags

   MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET15b-RvLEAMshort

   Plasmid

   #218122

   Purpose

   Expresses truncated RvLEAM protein under IPTG induction in bacterial host cells.

   Depositor

   Ci Ji Lim

   Article

   Abe et al bioRxiv [Preprint]. 2024 Feb 11:2024.02.

   Insert

   RvLEAMshort
   

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Truncated form (58-181aa)

   Promoter

   T7

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLBH531_MBP-Cas14a1 expression

    Plasmid

    #112500

    Purpose

    Expresses MBP fused to Cas14a1

    Depositor

    Jennifer Doudna

    Article

    Harrington et al Science. 2018 Oct 18. pii: science.aav42

    Insert

    Cas14a1
    

    Use

    CRISPR

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDB327

    Plasmid

    #113671

    Purpose

    expresses Ulp1_R3 in Escherichia coli

    Depositor

    Christopher Bahl

    Article

    Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

    Insert

    Ulp1_R3
    

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    contains solubility enhancing mutations

    Promoter

    T7

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pD-MSP1D1

    Plasmid

    #161607

    Purpose

    Bacterial expression of mutated membrane scaffold protein ("dark" MSP1D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

    Insert

    D-MSP1D1
    

    Tags

    7-His tag

    Expression

    Bacterial

    Mutation

    mutant of MSP1D1; all tryptophans were replaced b…

    Promoter

    T7

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSET FlipSuc-90u delta1

    Plasmid

    #14475

    Depositor

    Wolf Frommer

    Article

    Lager et al J Biol Chem. 2006 Oct 13. 281(41):30875-

    Insert

    FlipThuE W283A
    

    Tags

    ECFP and EYFP

    Expression

    Bacterial

    Mutation

    N192D, W283A, 13 amino acids deleted from linkers

    Promoter

    T7

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCI-T7Max-UTR1-deGFP-8xHis-T500

    Plasmid

    #178422

    Purpose

    Expression of deGFP via a T7 Promoter in TXTL

    Depositor

    Kate Adamala

    Article

    Deich et al J Biol Eng. 2023 Jan 23;17(1):4. doi: 10

    Insert

    deGFP
    

    Use

    Synthetic Biology

    Tags

    8xHis Tag

    Expression

    Bacterial

    Promoter

    T7Max

    Available Since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNCS Citrine

    Plasmid

    #91761

    Purpose

    Bacterial expression of a fluorescent protein, Citrine

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

    Insert

    Citrine
    

    Tags

    Hexa-Histidine tag, Xpress epitope for detection …

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBad-PhoCl2c

    Plasmid

    #164035

    Purpose

    Improved PhoCl variant with enhanced dissociation efficiency

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    PhoCl2c
    

    Tags

    His tag, T7 tag, Xpress Tag

    Expression

    Bacterial

    Promoter

    araBad promotor

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-mCherry-Rheb

    Plasmid

    #192436

    Purpose

    Encodes mCherry-tagged wild-type Rheb.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    mCherry-Rheb (RHEB Human)
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBroad-T7RNAP-chlorR

    Plasmid

    #199992

    Purpose

    T7 RNAP circuit variant T15 on ultra broad host range vector (RSF1010 and pAMB1 origins) chlorR

    Depositor

    Farren Isaacs

    Article

    Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

    Insert

    T7RNAP Circuit
    

    Expression

    Bacterial

    Mutation

    copFnull

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPdX

    Plasmid

    #85084

    Purpose

    Expresses putidaredoxin in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    Putidaredoxin
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    changed Cysteine 73 to Serine and Cysteine 85 to …

    Promoter

    T7 promoter

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNCS-mRuby3

    Plasmid

    #74234

    Purpose

    Constitutive expression of mRuby3 in bacteria

    Depositor

    Michael Lin

    Article

    Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

    Insert

    mRuby3
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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