8,969 results

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pLOVE-miR302/367

Plasmid

#31310

Depositor

Ed Morrisey

Article

Anokye-Danso et al Cell Stem Cell. 2011 Apr 8. 8(4):376-88.

Insert

mmu-miR302/367

Use

Lentiviral

Expression

Mammalian

Mutation

None

Available Since

Oct. 25, 2011

Availability

Academic Institutions and Nonprofits only
pJL43.1 - Pglh-2::MosTase::glh-2utr

Plasmid

#19332

Depositor

Erik Jorgensen

Article

Christian et al

Insert

Pglh-2::Mos1 transposase

Expression

Worm

Available Since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only

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MIP 339 CoMiP coLin28 2A Nanog IRES eGFP

Plasmid

#63729

Purpose

To express codon optimized Lin-28 and wild type Nanog. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).

Depositor

Mark Kay, Joseph Wu

Article

Diecke et al Sci Rep. 2015 Jan 28;5:8081. doi: 10.103

Inserts

Lin28 (LIN28A Human)
Nanog (NANOG Human)

Tags

T2A

Expression

Mammalian

Mutation

codon optimized

Promoter

SFFV

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Rheb-N153T

Plasmid

#19997

Depositor

Fuyuhiko Tamanoi

Article

Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

Insert

Ras homolog enriched in brain 1 - N153T (RHEB Human)

Tags

FLAG

Expression

Mammalian

Mutation

Changed Asparagine 153 to Threonine

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
MAC-tag-C

Plasmid

#108077

Purpose

Gateway compatible destination vectors containing StrepIII/HA/BioID

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

CCDB (ccdB(letD) )

Tags

StrepIII-HA-BirA*

Expression

Bacterial and Mammalian

Available Since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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CX-nestinGFPas

Plasmid

#32401

Depositor

Patrick Salmon, Henryk Zulewski

Article

Eberhardt et al Biochem Biophys Res Commun. 2006 Jul 7;3

Insert

nestin promoter/enhancer GFP

Use

Lentiviral

Available Since

June 19, 2013

Availability

Academic Institutions and Nonprofits only

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DsRed-rab9 WT

Plasmid

#12677

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB9 (Homo sapiens) (RAB9A Human)

Tags

DsRed and HA

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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Flag-HA-DUB3

Plasmid

#22593

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

DUB3 (USP17L9P Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 12, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15-UbE2W

Plasmid

#15809

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2W (UBE2W Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pET His6 MBP N10 TEV LIC cloning vector (2C-T)

Plasmid

#29706

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6-MBP-N10-TEV

Expression

Bacterial

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pDONR207 SARS-CoV-2 NSP1

Plasmid

#141255

Purpose

Gateway-compatible Entry vector, with insert of NSP1 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP1 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pAMPK alpha2 WT

Plasmid

#15991

Depositor

Morris Birnbaum

Article

Mu et al Mol Cell. 2001 May . 7(5):1085-94.

Insert

AMPK alpha2 (Prkaa2 Rat)

Tags

Myc

Expression

Mammalian

Available Since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only

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pBOB-CAG-SARS-CoV2-Spike D614G-HA

Plasmid

#158761

Purpose

3rd Generation lentiviral vector expressing the codon optimized SARS-CoV2 Spike Glycoprotein D614G high infectivity mutant with a C-terminal HA tag generated by Junko Ogawa & Gerald M Pao

Depositor

Gerald Pao

Article

Ogawa et al bioRxiv. 2020 Jul 22. doi: 10.1101/2020.

Insert

SARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Spike D614G

Promoter

CAG

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA flag PPAR gamma E499Q

Plasmid

#8896

Depositor

Bruce Spiegelman

Article

Hauser et al J Biol Chem. 2000 Jun 16. 275(24):18527-

Insert

PPAR gamma (Pparg Mouse)

Tags

flag

Expression

Mammalian

Mutation

E499Q, a mutant in the AF2 domain. Binds PPARg l…

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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pBABE puro SDF-1 alpha

Plasmid

#12270

Depositor

Bob Weinberg

Article

Orimo et al Cell. 2005 May 6. 121(3):335-48.

Insert

SDF-1 alpha (CXCL12 Human)

Use

Retroviral

Expression

Mammalian

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
tol2-mpx-mCherry

Plasmid

#29585

Depositor

Anna Huttenlocher

Article

Yoo et al Dev Cell. 2010 Feb 16. 18(2):226-36.

Insert

mpx-mCherry

Use

Zebrafish expression

Available Since

June 3, 2011

Availability

Academic Institutions and Nonprofits only
pVITRO1-M80-F2-IgG1/κ

Plasmid

#50383

Purpose

Expresses human IgG1/κ antibody isotype with unknown specificity. Useful vector for cloning of human antibodies with kappa light chain

Depositor

Andrew Beavil

Article

Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

Inserts

human Gamma 1 heavy chain expression cassette with unknown specificity
human Kappa light chain expression cassette with unknown specificity

Expression

Mammalian

Promoter

Mouse Elongation Factor 1 Alpha and Rat Elongatio…

Available Since

Jan. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pHYRS52

Plasmid

#31122

Depositor

Hideo Iwai

Article

Muona et al Chembiochem. 2008 Dec 15. 9(18):2958-61.

Insert

Ulp1 (ULP1 Budding Yeast)

Tags

His

Expression

Bacterial

Mutation

Ulp1(403-621)

Available Since

Sept. 25, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 MEKK1 WT

Plasmid

#12181

Depositor

Gary Johnson

Article

Widmann et al Mol Cell Biol. 1998 Apr . 18(4):2416-29.

Insert

MEKK1 (Map3k1 Mouse)

Tags

His and Xpress

Expression

Mammalian

Mutation

V1467L

Available Since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pMCP-Luc (MCP-1 promoter in pGL3-basic)

Plasmid

#40324

Depositor

Alexander Dent

Article

Toney et al Nat Immunol. 2000 Sep;1(3):214-20.

Insert

MCP-1 promoter (Ccl2 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

contains MCP-1 promoter region through the nucleo…

Promoter

MCP-1

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-Brainbow-1.0 L

Plasmid

#18721

Depositor

Joshua Sanes

Article

Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

Insert

dTomato ; mCerulean ; EYFP

Use

Cre/Lox

Expression

Mammalian

Available Since

Aug. 15, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
single polypeptide FPX biosensor for calcium ion

Plasmid

#60887

Purpose

FPX biosensor

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

single polypeptide FPX biosensor for calcium ion

Tags

HindIII site

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pGG-B1

Plasmid

#78504

Purpose

Trans plasmid for production of recombinant AAV-B1 vectors. Plasmid backbone may yield higher titers than pAR-B1 and encodes AAV2 Rep and AAV-B1 capsid

Depositor

Miguel Sena-Esteves

Article

Choudhury et al Mol Ther. 2016 Apr 27. doi: 10.1038/mt.2

Inserts

AAV2 Rep
AAV-B1 capsid

Use

AAV

Expression

Mammalian

Available Since

June 20, 2016

Availability

Academic Institutions and Nonprofits only

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CF-Ins54p

Plasmid

#20155

Depositor

Tobias Meyer

Article

Suh et al Science. 2006 Dec 1. 314(5804):1454-7.

Insert

CFP-FKBP-Inp54p (INP54 Budding Yeast)

Expression

Mammalian

Mutation

Stop codon at position 331.

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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pBiFC-bJunVN155(I152L)

Plasmid

#27098

Depositor

Chang-Deng Hu

Article

Kodama et al Biotechniques. 2010 Nov . 49(5):793-805.

Insert

bJunVN155(I152L) (Jun and Aequorea Victoria, Rat)

Tags

Myc

Expression

Mammalian

Mutation

I152L in Venus(1-154)

Available Since

Jan. 13, 2011

Availability

Academic Institutions and Nonprofits only

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K44A dynamin 1 pEGFP

Plasmid

#34681

Depositor

Sandra Schmid

Article

Song et al Mol Biol Cell. 2004 May;15(5):2243-52. E

Insert

dynamin 1ba (DNM1L Human)

Tags

EGFP

Expression

Mammalian

Mutation

K44A

Available Since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pARF6-CFP

Plasmid

#11382

Depositor

Joel Swanson

Article

Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

Insert

ADP Ribosylation Factor 6 (ARF6 Human)

Tags

CFP

Expression

Mammalian

Available Since

March 3, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pBT378_pattB-pCA-GFP-pA-attB

Plasmid

#52554

Purpose

for PhiC31 integrase-mediated cassette exchange

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP

Expression

Mammalian

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pQLinkH

Plasmid

#13667

Depositor

Konrad Buessow

Article

Scheich et al Nucleic Acids Res. 2007;35(6):e43. doi:

Insert

multi cloning site

Use

Co-expression

Tags

His, TEV, and attB1

Expression

Bacterial

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
tetO-GATA4

Plasmid

#46030

Depositor

John Gearhart

Article

Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

Insert

GATA4 (GATA4 Human)

Use

Lentiviral

Tags

His and V5

Expression

Mammalian

Mutation

R43Q, G50S and N197S

Promoter

tetO

Available Since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge

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8,969 results