164,827 results

1. pcDNA3-ApaLID211A-HA

   Plasmid

   #211459

   Purpose

   Express mitochondrial localized ApaLI with D211A mutation

   Depositor

   Agnel Sfeir

   Article

   Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

   Insert

   ApaLI-D211A
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D211A

   Promoter

   CMV

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. 4xM67 pTATA TK-Luc

   Plasmid

   #8688

   Depositor

   Jim Darnell

   Article

   Besser et al Mol Cell Biol 1999 Feb;19(2):1401-9.

   Insert

   4 repeated M67 sites
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 18, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. GCH1_pLX307

   Plasmid

   #98336

   Purpose

   Lentiviral expression of GCH1

   Depositor

   William Hahn, Sefi Rosenbluh

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   GCH1 (GCH1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   E1Fa

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3Venus

   Plasmid

   #61629

   Purpose

   R2D2: Expresses a fusion of auxin-sensitive DII to nuclear 3xVenus and auxin-insensitive mDII to nuclear tdTomato, both from the Arabidopsis RPS5A promoter

   Depositor

   Dolf Weijers

   Article

   Liao et al Nat Methods. 2015 Mar;12(3):207-10. doi:

   Inserts

   mDII
   ntdTomato
   DII
   n3xVenus
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   RPS5A

   Available Since

   March 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX-PVX

   Plasmid

   #213040

   Purpose

   PVX vector to express heterologous sequences in plants

   Depositor

   Jose-Antonio Daros

   Article

   Uranga et al Hortic Res. 2023 Dec 19;11(1):uhad279. d

   Insert

   Potato virus X
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Cauliflower mosaic virus 35S

   Available Since

   May 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti_P2A_Puro

   Plasmid

   #211364

   Purpose

   Lentiviral expression vector for an insert of interest linked to a P2A-T2A puromycin sequence. Produces virus very efficiently. Can also be used for regular transfections

   Depositor

   Jacques Neefjes

   Article

   Middelburg et al Cell Rep. 2023 Dec 26;42(12):113516. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDIV272

   Plasmid

   #226183

   Purpose

   Expresses Cas9 and gRNA targeting uidA gene of the pDIV643 derived cassette.

   Depositor

   Uffe Mortensen

   Article

   Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

   Insert

   tRNA-sgRNA-tRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   TEF1p (Komagataella phaffii)

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAcGFP1-N1-Dsup

   Plasmid

   #90020

   Purpose

   To express GFP-tagged Dsup in mammalian cells transiently

   Depositor

   Takekazu Kunieda

   Article

   Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

   Insert

   Dsup
   

   Use

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF3298

   Plasmid

   #144774

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   EZH2 (EZH2 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-TDP-43

    Plasmid

    #235487

    Purpose

    Expression of human TDP-43-EGFP

    Depositor

    Maxime Rousseaux

    Article

    Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

    Insert

    TARDBP (TARDBP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 30, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pHAGE2-EF1a-HA-ATAD3A-IRES-Hygro

    Plasmid

    #211469

    Purpose

    Lentivirus backbone expressing ATAD3A with an HA-tag at the N-terminal

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    ATAD3A (ATAD3A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMRX-IZ-HaloTag7-CHMP4A

    Plasmid

    #184895

    Purpose

    Stable expression of HaloTag7-CHMP4A in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

    Insert

    CHMP4A (CHMP4A Human)
    

    Use

    Tags

    HaloTag7

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRRlsinPGK_H2BGFP_WPRE

    Plasmid

    #91788

    Purpose

    Constitutive expression of H2BGFP fusion using PGK promoter in a lentiviral backbone.

    Depositor

    Beverly Torok-Storb

    Insert

    Human histone H2B (H2BC11 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-GST

    Plasmid

    #42049

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    GST
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-HA-LMW-FGF2

    Plasmid

    #157663

    Purpose

    Expresses FGF2 in mammalian cell

    Depositor

    Byung Il Lee

    Article

    Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

    Insert

    Fibroblast growth factor 2 (FGF2 Human)
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    deleted amino acid 1-134

    Promoter

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV SCH9

    Plasmid

    #212708

    Purpose

    A SCHEMA evolved AAV variant effective at targeting subventricular NSCs

    Depositor

    David Schaffer

    Article

    Ojala et al Mol Ther. 2018 Jan 3;26(1):304-319. doi:

    Insert

    AAV2 Rep SCH9 Cap
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3 H2B-mIFP T2A Mpro (wt)

    Plasmid

    #163079

    Purpose

    Expresses wild type Mpro (active) and a near-infrared fluorescent protein mIFP fused to H2B

    Depositor

    Xiaokun Shu

    Insert

    Wild type Mpro of SARS-CoV-2 and H2B-mIFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. DLX2-hygro

    Plasmid

    #97330

    Purpose

    Generating lentiviruses to express Dlx2 and hygromycin resistance

    Depositor

    Marius Wernig

    Article

    Yang et al Nat Methods. 2017 May 15. doi: 10.1038/n

    Insert

    DLX2 (Dlx2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TetO

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETM6-mCherry

    Plasmid

    #66534

    Purpose

    Expresses mCherry under the control of the Concensus T7 Promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon Optimized for E. coli

    Promoter

    Consensus T7

    Available Since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEMS-II

    Plasmid

    #204765

    Purpose

    GEMS sensor system (pCMV-EGFP-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577)

    Depositor

    Kate Meyer

    Article

    Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    EGFP-m6Alinker-DHFR
    

    Use

    Tags

    Expression

    Mutation

    mutated all RACs in EGFP to RGC/RCC

    Promoter

    CMV

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLenti HRE-Luc pGK Hygro

    Plasmid

    #118706

    Purpose

    reporter plasmid HIF-responsive promoter (3xHRE) wt- fused to firefly luciferase

    Depositor

    William Kaelin

    Article

    Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

    Insert

    HRE (HIF-responsive promoter (3xHRE)-luc
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. PRK5-FLAG-Bag6ΔUBL

    Plasmid

    #61837

    Purpose

    express N-terminally FLAG tagged human Bag6 without UBL domain

    Depositor

    Yihong Ye

    Article

    Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

    Insert

    Bag6 (BAG6 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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23. pWXC078

    Plasmid

    #222961

    Purpose

    C. elegans codon-optimized mScarlet-I3 with 3 introns cloned into pPD95.75 backbone

    Depositor

    Oliver Hobert

    Article

    Cao et al Genetics. 2024 Oct 7;228(2):iyae126. doi

    Insert

    mScarlet-I3
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. Flag-HA-USP30

    Plasmid

    #22578

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP30 (USP30 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available Since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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25. pLEX305_FKBP12F36V-SHOC2

    Plasmid

    #134522

    Purpose

    Lentiviral plasmid expressing SHOC2 with N-term FKBP12(F36V) tag

    Depositor

    Andrew Aguirre

    Article

    Sulahian et al Cell Rep. 2019 Oct 1;29(1):118-134.e8. d

    Insert

    SHOC2 (SHOC2 Human)
    

    Use

    Lentiviral

    Tags

    FKBP12F36V-2xHA

    Expression

    Mammalian

    Mutation

    Silent mutations made at wobble base position at …

    Promoter

    hPGK

    Available Since

    Nov. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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26. pcDNA3.1(+)-HA-Flag-natT1R2

    Plasmid

    #113944

    Purpose

    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R2 (TAS1R2 Human)
    

    Use

    Tags

    FLAG and Signal/leader sequence from influenza he…

    Expression

    Mammalian

    Mutation

    residues 22-839

    Promoter

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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27. pmCherry-C1 mCherry-NLS

    Plasmid

    #58476

    Purpose

    Expresses a nuclear-targeted mCherry control construct, on a CMV reporter

    Depositor

    Dyche Mullins

    Article

    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

    Insert

    3X NLS with 3X stop codons
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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28. pLVX_DSB_Spectrum-V3

    Plasmid

    #192476

    Purpose

    Lentiviral DSB Spectrum V3 Reporter System

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    BFP1.2, mCherry, incompleteGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHREAC

    Plasmid

    #134908

    Purpose

    Overexpression of recombinant proteins in plants thanks to very efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites. Download fully annotated map under Resource Information.

    Depositor

    George Lomonossoff

    Article

    Peyret et al Plant Methods. 2019 Sep 18;15:108. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    double 35S

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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30. pHAGE-CMV-Luc2-IRES-ZsGreen-W

    Plasmid

    #164432

    Purpose

    Lentiviral vector backbone encoding firefly luciferase and ZsGreen

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Inserts

    Luciferase
    ZsGreen
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge