159,352 results

1. HDAC4 Flag

   Plasmid

   #13821

   Purpose

   Mammalian expression of human histone deacetylase 4 with flag tag

   Depositor

   Eric Verdin

   Article

   Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

   Insert

   HDAC4 (HDAC4 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAVS1-P-CAG-DEST

   Plasmid

   #80490

   Purpose

   Gateway Destination donor vector for AAVS1 targeting (puromycin selection)

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Type

   Empty backbone

   Use

   Donor vector (human)

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLX-EF1a_GFP

   Plasmid

   #221481

   Purpose

   GFP lentiviral overexpression

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCh-Sec61 beta

   Plasmid

   #49155

   Purpose

   expression of Sec61 beta fused to mCherry, used as a general ER marker

   Depositor

   Gia Voeltz

   Article

   Zurek et al Traffic. 2011 Jan;12(1):28-41. doi: 10.1

   Insert

   Sec61 beta (SEC61B Human)
   

   Use

   Tags

   mCherry*

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. eIF2a 1

   Plasmid

   #21807

   Depositor

   David Ron

   Insert

   eIF2a (EIF2S1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. myc-mTOR

   Plasmid

   #1861

   Depositor

   David Sabatini

   Article

   Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

   Insert

   mTOR (Mtor Rat)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQC-mCherryFP-GFP

   Plasmid

   #129102

   Purpose

   mCherryFP positive control

   Depositor

   Fangliang Zhang

   Article

   Birnbaum et al Cell Chem Biol. 2018 Aug 16;25(8):1038-1

   Insert

   mCherryFP
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF3015

   Plasmid

   #144491

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PPARA (PPARA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. PM PKA Reporter

   Plasmid

   #104551

   Purpose

   Expresses Lyn-mTurquoise2-VASP at the plasma membrane of mammalian cells

   Depositor

   David Lawrence

   Article

   O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

   Insert

   Lyn-mTurquoise2-VASP 148 - 164
   

   Use

   Tags

   mTurquoise 2, HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1 alpha

   Available since

   March 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. PT2/C-Luc//PGK-SB13

    Plasmid

    #20207

    Depositor

    John Ohlfest

    Article

    Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

    Insert

    Flirefly luciferase/Sb transposase
    

    Use

    Transposon

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEntr_Myc-BirA-G3-ER

    Plasmid

    #173814

    Purpose

    Promiscuous biotin ligase (BirA-G3) targeted to ER lumen in Gateway entry vector. N-terminal secretion signal from Drosophila BiP. C-terminal KDEL. Myc tag inserted after sig. seq. cleavage site

    Depositor

    Norbert Perrimon

    Article

    Droujinine et al Nat Commun. 2021 Apr 22;12(1):2382. doi:

    Insert

    Myc-BirA-G3-ER
    

    Use

    Gateway shuttle vector

    Tags

    Myc

    Expression

    Mutation

    Promoter

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-EF1a-Con/Fon-GCaMP6M

    Plasmid

    #137119

    Purpose

    Intersectional viral expression of GCaMP6M in cells expressing both Cre AND Flp

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Fon-GCaMP6M
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    Removed RSET tag

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZS157 CRISPEY RT/Cas9

    Plasmid

    #114454

    Purpose

    Yeast Integration Plasmid for galactose inducible Ec86-RT and SpCas9 expression

    Depositor

    Hunter Fraser

    Article

    Sharon et al Cell. 2018 Sep 18. pii: S0092-8674(18)31

    Insert

    SpCas9, Ec86-RT
    

    Use

    CRISPR

    Tags

    SV40-NLS

    Expression

    Yeast

    Mutation

    S. cerevisiae codon optimized

    Promoter

    GAL1-GAL10

    Available since

    Oct. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-PV-NLS-GFP

    Plasmid

    #17300

    Depositor

    Anton Bennett

    Article

    Pusl et al J Biol Chem. 2002 Jul 26. 277(30):27517-

    Insert

    PV-GFP-NLS (Pvalb Rat)
    

    Use

    Tags

    Myc and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDisplay-pHuji

    Plasmid

    #61556

    Purpose

    Red fluorescent protein based pH indicator in pDisplay vector

    Depositor

    Robert Campbell

    Article

    Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

    Insert

    pHuji
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMAL-p5X

    Plasmid

    #150814

    Purpose

    Expresses proteins in the periplasm as fusions to a high-binding mutant (A313V) maltose-binding protein

    Depositor

    Paul Riggs

    Type

    Empty backbone

    Use

    Tags

    malE A313V

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 22, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pAAV-CMV-EGFP-NLS

    Plasmid

    #165441

    Purpose

    Expresses nuclear EGFP from CMV promoter

    Depositor

    Esteban Engel

    Article

    Maturana et al Gene Ther. 2021 Jun 28. pii: 10.1038/s41

    Insert

    EGFP-NLS
    

    Use

    AAV

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. APOE_Halo_C_allele

    Plasmid

    #178121

    Purpose

    Donor vector for endogenous tagging of human APOE at the C-terminus with halotag

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Insert

    Halotag (APOE Human)
    

    Use

    Donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-KASH2

    Plasmid

    #187017

    Purpose

    Inducible disruption of LINC Complex

    Depositor

    Jan Lammerding

    Article

    Earle et al Nat Mater. 2019 Dec 16. pii: 10.1038/s41

    Insert

    KASH2
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCRISPomyces-2

    Plasmid

    #61737

    Purpose

    Streptomyces expression of codon-optimized Cas9 and custom gRNA

    Depositor

    Huimin Zhao

    Article

    Cobb et al ACS Synth Biol. 2014 Dec 8.

    Inserts

    sSpCas9
    gRNA cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    BbsI-flanked lacZ cassette inserted in place of s…

    Promoter

    gapdhp(EL) and rpsL(XC)-BbsI

    Available since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. pPOTv7-blast-10V5

    Plasmid

    #181938

    Purpose

    Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

    Depositor

    Samuel Dean

    Article

    Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

    Insert

    10xV5
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    read through

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCAG-CFP

    Plasmid

    #11179

    Purpose

    Mammalian expression vector for expression of CFP

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

    Insert

    ECFP from Aequorea victoria
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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23. pKnockinDonor-human-p16INK4A-P2A-mCherry

    Plasmid

    #137026

    Purpose

    donor vector to knock in P2A-mCherry to the C-terminus of endogenous human p16INK4A

    Depositor

    Chunliang Li

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2019 Dec 9. pi

    Insert

    p16INK4A-knockin-homology arm-P2A-mCherry
    

    Use

    Tags

    P2A-mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    no promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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24. EGFP-CLC

    Plasmid

    #193559

    Purpose

    Expresses human Clathrin Light Chain B tagged with mEGFP in mammalian cells.

    Depositor

    Justin Taraska

    Article

    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

    Insert

    Clathrin light chain B (CLTB Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pNCS mCherry

    Plasmid

    #91769

    Purpose

    Bacterial expression of a fluorescent protein, monomeric Cherry (mCherry)

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

    Insert

    monomeric Cherry
    

    Use

    Tags

    Hexa-Histidine tag, Xpress epitope for detection …

    Expression

    Bacterial

    Mutation

    See Depositor Comments

    Promoter

    T7

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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26. Ubi:Zebrabow

    Plasmid

    #118219

    Purpose

    For ubiquitous expression of Brainbow in zebrafish

    Depositor

    Albert Pan, Alex Schier

    Article

    Pan et al Development. 2013 Jul;140(13):2835-46. d

    Insert

    Brainbow 1.0 L
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLV-UbC-Tet1c-dCas9

    Plasmid

    #232178

    Purpose

    Lentiviral cassette that expresses Tet1c-dCas9 fusion with a short linker in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

    Insert

    Tet1 catalytic domain - dead Cas9 - 2A - BlastR (TET1 S. pyogenes, Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    hUbC

    Available since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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28. PSMA4_GFP_Cterm

    Plasmid

    #221514

    Purpose

    Fluorescently tagged Psma4-GFP

    Depositor

    Alessandro Ori

    Insert

    PSMA4 (PSMA4 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET22b-CPDBamHI-Leu

    Plasmid

    #38256

    Depositor

    Aimee Shen, Matthew Bogyo

    Article

    Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

    Type

    Empty backbone

    Use

    Tags

    6xHis and Vibrio cholerae MARTX toxin cysteine pr…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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30. pBabe-Dvl2-GFP

    Plasmid

    #109148

    Purpose

    Stable Dvl2 expression

    Depositor

    Mariann Bienz

    Article

    Gammons et al J Cell Sci. 2016 Oct 15;129(20):3892-390

    Insert

    Dvl2 (DVL2 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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