We narrowed to 28,416 results for: REP

1. pTK_BsmBI_LacZ_Citrine_Nanotag_35

   Plasmid

   #130524

   Purpose

   Enhancer cloning nanotag reporter vector

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

   Insert

   LacZ
   

   Use

   Enhancer cloning nanotag reporter vector

   Tags

   Expression

   Mutation

   Promoter

   thymidine kinase minimal promoter

   Available since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTK_BsmBI_LacZ_Citrine_Nanotag_18

   Plasmid

   #130519

   Purpose

   Enhancer cloning nanotag reporter vector

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

   Insert

   LacZ
   

   Use

   Enhancer cloning nanotag reporter vector

   Tags

   Expression

   Mutation

   Promoter

   thymidine kinase minimal promoter

   Available since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTK_BsmBI_LacZ_Citrine_Nanotag_17

   Plasmid

   #130518

   Purpose

   Enhancer cloning nanotag reporter vector

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

   Insert

   LacZ
   

   Use

   Enhancer cloning nanotag reporter vector

   Tags

   Expression

   Mutation

   Promoter

   thymidine kinase minimal promoter

   Available since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTK_BsmBI_LacZ_Citrine_Nanotag_11

   Plasmid

   #130517

   Purpose

   Enhancer cloning nanotag reporter vector

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

   Insert

   LacZ
   

   Use

   Enhancer cloning nanotag reporter vector

   Tags

   Expression

   Mutation

   Promoter

   thymidine kinase minimal promoter

   Available since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFastBac K-Flag M33

   Plasmid

   #1992

   Depositor

   Robert Kingston

   Article

   Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

   Insert

   M33 (Cbx2 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Insect

   Mutation

   Kozak sequence added before ATG

   Promoter

   Available since

   March 23, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pX459V2.0+K848A

   Plasmid

   #108296

   Purpose

   pX459 V2.0 (Plasmid #62988) with the K848A mutation

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGDP1 vph

   Plasmid

   #112893

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   vph
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Pbla

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac K-M33-Flag

   Plasmid

   #1993

   Depositor

   Robert Kingston

   Article

   Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

   Insert

   M33 (Cbx2 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Insect

   Mutation

   Kozak sequence added before ATG

   Promoter

   Available since

   March 23, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pET-28c-F30-2xdBroccoli-47CAG

   Plasmid

   #237217

   Purpose

   Express F30-2xdBroccoli tagged 47 repeats of CAG RNA in bacteria.

   Depositor

   Mingxu You

   Article

   Xue et al Nucleic Acids Res. 2023 Sep 8;51(16):833

   Insert

   47 CAG trinucleotide repeats RNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-PM-deltaCKAR

    Plasmid

    #222429

    Purpose

    FRET-based reporter for monitoring deltaPKC activity at the plasma membrane in cells.

    Depositor

    Alexandra Newton

    Article

    Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

    Insert

    Plasma membrane delta-selective C kinase activity reporter
    

    Use

    Tags

    CFP and YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-Nuc-deltaCKAR

    Plasmid

    #222430

    Purpose

    FRET-based reporter for monitoring deltaPKC activity at the nucleus in cells.

    Depositor

    Alexandra Newton

    Article

    Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

    Insert

    Nucleus delta-selective C kinase activity reporter
    

    Use

    Tags

    CFP and YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-Golgi-deltaCKAR

    Plasmid

    #222428

    Purpose

    FRET-based reporter for monitoring deltaPKC activity at the Golgi in cells.

    Depositor

    Alexandra Newton

    Article

    Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

    Insert

    Golgi delta-selective C kinase activity reporter
    

    Use

    Tags

    CFP and YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCDNA3-2xMARS-PLD

    Plasmid

    #205237

    Purpose

    Expresses two repeats of PLEKAH5 aa 143-271 (K163A and R164A) fused to phospholipase D from Streptomyces sp. PMF

    Depositor

    Jeremy Baskin

    Inserts

    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    phospholipase D (PLD)
    

    Use

    Tags

    Nuclear Export Sequence and mCherry

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    CMV

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRAR119

    Plasmid

    #185051

    Purpose

    J23108(spel), pHP14 stability hairpin, strong RBS, L34 coding region, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L34
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTcI39-PRM-wasabi

    Plasmid

    #195555

    Purpose

    Temperature-dependent expression of mWasabi reporter with transcription activation by TcI39

    Depositor

    Mikhail Shapiro

    Article

    Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

    Inserts

    mWasabi
    TcI39
    

    Use

    Tags

    6x His and AANDENYADAS degradation tag

    Expression

    Bacterial

    Mutation

    A67T

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Modular BE4max for GG insertion

    Plasmid

    #198891

    Purpose

    Easily insert deaminase of choice into BE4max using Golden Gate cloning

    Depositor

    Reuben Harris

    Article

    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

    Insert

    CRISPR associated protein 9 (cas9 Streptococcus pyogenes)
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLY171

    Plasmid

    #184145

    Purpose

    sgRNA with 3 RNA aptemers BoxB at the 3'-end for CRISPRa

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Insert

    sgRNA-LEA2-ex3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Plux2

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. SH2-free

    Plasmid

    #175800

    Purpose

    Human Nck2 SH2 domain

    Depositor

    Barry Stoddard

    Article

    Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi:

    Insert

    human Nck2 SH2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TetO-FUW-FLAG-NLS-KRAB-MCS_N-term

    Plasmid

    #136888

    Purpose

    Doxycycline-inducible vector backbone containing the KRAB repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)

    Depositor

    Moritz Mall

    Article

    Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

    Insert

    KRAB repressor domain
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TetO-FUW-MCS-KRAB_C-term

    Plasmid

    #136889

    Purpose

    Doxycycline-inducible vector backbone containing a multiple cloning site (MCS), the KRAB repressor domain and a STOP codon

    Depositor

    Moritz Mall

    Article

    Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

    Insert

    KRAB repressor domain
    

    Use

    Lentiviral

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PB-TAC-Cas9

    Plasmid

    #120352

    Purpose

    piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). SpCas9 is constitutively expressed.

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Type

    Empty backbone

    Use

    CRISPR; Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tet-on and CBh

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PB-TAC-KRAB-dCas9

    Plasmid

    #120355

    Purpose

    piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). KRAB-dCas9 is constitutively expressed.

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Type

    Empty backbone

    Use

    CRISPR; Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tet-on and CBh

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCbh-SpyCas9-2A-puro-pA_U6-sgRosa26

    Plasmid

    #149346

    Purpose

    Mammalian expression vector for SpyCas9, puromycin resistance and mouse Rosa26 guide RNA

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    Cbc

    Available since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCbh-SpyCas9-2A-puro-pA_U6-BbsI

    Plasmid

    #149347

    Purpose

    Mammalian expression vector for SpyCas9 and puromycin resistance

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    SpyCas9
    

    Use

    CRISPR

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. BII-C3H

    Plasmid

    #133386

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. BII-B3HTK

    Plasmid

    #133389

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTK_Cerulean_Nanotag_92_positive_Control

    Plasmid

    #130573

    Purpose

    Enhancer cloning nanotag reporter vector

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

    Insert

    FoxD3_cranial_neural_crest_enhancer_NC4
    

    Use

    Enhancer cloning nanotag reporter vector

    Tags

    Expression

    Mutation

    Promoter

    thymidine kinase minimal promoter

    Available since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTK_Cerulean_Nanotag_91_positive_Control

    Plasmid

    #130572

    Purpose

    Enhancer cloning nanotag reporter vector

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

    Insert

    FoxD3_cranial_neural_crest_enhancer_NC3
    

    Use

    Enhancer cloning nanotag reporter vector

    Tags

    Expression

    Mutation

    Promoter

    thymidine kinase minimal promoter

    Available since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. KA1270_pPB-gPou5f1_3.5kb-UBCmin-IP

    Plasmid

    #124181

    Purpose

    piggyBac-based reporter (Puro) driven by the Pou5f1 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    IRES-Pac, Pou5f1 enhancer
    

    Use

    Reporter

    Tags

    Expression

    Mutation

    Promoter

    UBCmin

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. KA1265_pPB-gPou5f1_3.5kb-UBCminEGFP-IP

    Plasmid

    #124180

    Purpose

    piggyBac-based reporter (EGFP) driven by the Pou5f1 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    EGFP-IRES-Pac, Pou5f1 enhancer
    

    Use

    Reporter

    Tags

    Expression

    Mutation

    Promoter

    UBCmin

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge