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We narrowed to 30,195 results for: REP

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Showing: 7441 - 7470 of 30195 results

  1. p189

    Plasmid
    #65207
    Purpose
    Mutant EGFP expression plasmid for the creation of a heteroduplex DNA repair assay plasmid with pGEM5Z(+)-EGFP (p111). *Please see notes in comments section.
    Depositor
    LuZhe Sun
    Article
    Zhou et al Anal Biochem. 2009 May 1;388(1):167-9. d
    Insert
    EGFP
    Expression
    Bacterial and Mammalian
    Mutation
    Premature stop codon added to pGEM5Z(+)-EGFP (TGG…
    Promoter
    CMV
    Available Since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    65207-plasmid-map-sequence-id-490957

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  7. pCB-1

    Plasmid
    #210387
    Purpose
    All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces
    Depositor
    Gilles van Wezel
    Article
    Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021
    Inserts
    dCas9
    CymR
    sgRNA cassette
    Use
    CRISPR; Integrative vector for use in streptomyces
    Mutation
    BsaI-flanked RFP cassette inserted in place of sp…
    Promoter
    SP11 and SP30
    Available Since
    Jan. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    210387-plasmid-map-sequence-id-418472

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  11. SP6-VEE-GFP

    Plasmid
    #58976
    Purpose
    expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon
    Depositor
    Steven Dowdy
    Article
    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.
    Insert
    EGFP
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Expression
    Mammalian
    Promoter
    26S subgenomic promoter
    Available Since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    58976-plasmid-map-sequence-id-445055

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  12. pCB-2

    Plasmid
    #210388
    Purpose
    All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces
    Depositor
    Gilles van Wezel
    Article
    Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021
    Inserts
    dCas9
    CymR
    sgRNA cassette
    Use
    CRISPR; Integrative vector for use in streptomyces
    Mutation
    BsaI-flanked RFP cassette inserted in place of sp…
    Promoter
    SP11 and SP30
    Available Since
    Jan. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    210388-plasmid-map-sequence-id-418474

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  14. Cas9-sgRNA-A

    Plasmid
    #149369
    Purpose
    Expresses SpCas9 and sgRNA targeting the lenti-CDDR reporter
    Depositor
    Tarek Abbas
    Article
    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592
    Inserts
    Cas9
    sgRNA-A
    Puromycin resistance
    Use
    CRISPR
    Tags
    3xFLAG, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Promoter
    CBh, PGK, and U6
    Available Since
    Nov. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    149369-plasmid-map-sequence-id-313973

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  15. Cas9-sgRNA-A+B

    Plasmid
    #149370
    Purpose
    Expresses SpCas9 and two sgRNA targeting the lenti-CDDR reporter at two distal sites
    Depositor
    Tarek Abbas
    Article
    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592
    Inserts
    Cas9
    sgRNA-A
    sgRNA-B
    Puromycin resistance
    Use
    CRISPR
    Tags
    3xFLAG, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Promoter
    CBh, PGK, and U6
    Available Since
    Oct. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    149370-plasmid-map-sequence-id-315474

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  17. pGM55

    Plasmid
    #71249
    Purpose
    luciferase reporter with hGM-CSF -55 to +28 minimal promoter
    Depositor
    Peter Cockerill
    Article
    Bert et al Plasmid. 2000 Sep;44(2):173-82.
    Insert
    GM-CSF minimal promoter (-55 to +28) (CSF2 Human)
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Promoter
    GM-CSF minimal
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    71249-plasmid-map-sequence-id-131890

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  24. pGEM5Z(+)-EGFP

    Plasmid
    #65206
    Purpose
    EGFP expression plasmid (also called p111) for the creation of a heteroduplex DNA repair assay plasmid with p189. *Please see notes in comments section.
    Depositor
    LuZhe Sun
    Article
    Zhou et al Anal Biochem. 2009 May 1;388(1):167-9. d
    Insert
    EGFP
    Expression
    Bacterial and Mammalian
    Mutation
    Restriction enzyme recognition sites added either…
    Promoter
    CMV
    Available Since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    65206-plasmid-map-sequence-id-490956

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  25. pAM5188

    Plasmid
    #120087
    Purpose
    Cyanobacteria cloning vector based on Synechococcus elongatus PCC 7942 small plasmid, harboring a toxin-antitoxin cassette to improve the plasmid stability without antibiotic selection.
    Depositor
    Susan Golden
    Article
    Chen et al Microbiology. 2016 Dec;162(12):2029-2041
    Insert
    pAM4788 where the promoter/reporter device (PconII-LTRBS-yemGFP) was replaced with a MCS from the pBluescript vector (pBS MCS).
    Use
    Other
    Available Since
    Feb. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120087-plasmid-map-sequence-id-234428

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  26. pBS CMV NA 3C FLAG SA

    Plasmid
    #234993
    Purpose
    Expression of transmembrane region of Neuraminidase protein fused to streptavidin protein for displaying on viral like particles (VLPs) when cotranfected with GAG protein
    Depositor
    Monica Gonzalez-Magaldi, Daniel Leahy
    Article
    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.
    Insert
    NA
    Tags
    HRV 3C site, FLAG, Strep-Tactin (SA)
    Expression
    Mammalian
    Mutation
    In the Streptavidin coding sequence Glu44Val/Ser4…
    Available Since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    234993-plasmid-map-sequence-id-468948

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  27. pU6-Universal.Sth.dCas9

    Plasmid
    #171088
    Purpose
    CRISPR interference. Recipient construct for cloning 20-nt spacers into the sgRNA scaffold compatible with Streptococcus thermophilus dCas9 (CRISPR1 system) via BsaI restriction sites.
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Toxoplasma U6 upstream region – spacer cloning site - sgRNA scaffold (compatible with S. thermophilus Cas9 CRISPR1)
    DHFR-TSc3
    Use
    Toxoplasma gondii expression
    Mutation
    Note: A S245F mutation is present but did not app…
    Promoter
    DHFR-TS (Toxoplasma gondii) and U6 (Toxoplasma go…
    Available Since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171088-plasmid-map-sequence-id-335785

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  28. pSUC2::GNSI (NrsR)

    Plasmid
    #121537
    Purpose
    This plasmid contains a CYC1pr driven GNSI reporter and flanking homology to SUC2. Digestion with NotI, SacI, and EcoRV, allows integration at the SUC2 locus.
    Depositor
    Alexey Merz
    Article
    Plemel et al Traffic. 2020 Nov 23. doi: 10.1111/tra.1
    Inserts
    CYC1 Promoter (CYC1 Budding Yeast)
    Nyv1 Cytosolic Domain (aa 6-230)
    Snc1 Transmembrane Domain (TMD)
    SUC2 CDS
    SUC2 terminator
    NrsR
    Tags
    mGFP5
    Expression
    Yeast
    Mutation
    M109I (Naturally occurring SNP) and The first 21 …
    Available Since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    121537-plasmid-map-sequence-id-235299

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  29. PV225

    Plasmid
    #132334
    Purpose
    dCas9 plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea mays
    Depositor
    Joshua Young
    Article
    Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.
    Insert
    dCas9-AT-CBF1 (CBF1 Cas9 (Streptococcus pyogenes); AT-CBF1 (Arabidopsis thaliana))
    Tags
    AT-CBF1
    Expression
    Plant
    Mutation
    Codon optimize for expression and stability in Ze…
    Available Since
    Oct. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    132334-plasmid-map-sequence-id-258129

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Showing: 7441 - 7470 of 30195 results