166,224 results

1. pLL3.7-EF-EYFP-YAP1_WT-PolyA

   Plasmid

   #112284

   Purpose

   Fluorescently labels WT human YAP1-2gamma isoform (504 aa)

   Depositor

   Erik Sahai

   Article

   Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

   Insert

   EYFP-YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Mutation

   WT human YAP1-2gamma

   Promoter

   EF-1 alpha

   Available Since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. SP-dCas9-VPR

   Plasmid

   #63798

   Purpose

   SP-dCas9 with VP64-p65-Rta (VPR) fused to C-terminus; mammalian vector

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

   Insert

   SP-dCas9-VPR
   

   Use

   CRISPR and Synthetic Biology

   Tags

   VPR (VP64-p65-Rta)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV_ENVLPE+

   Plasmid

   #232427

   Purpose

   Expression plasmid to produce ENVLPE+ particles

   Depositor

   Gil Westmeyer

   Article

   Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

   Insert

   ENVLPE+
   

   Expression

   Mammalian

   Promoter

   2xCMV

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-PEmax

   Plasmid

   #174820

   Purpose

   Mammalian expression of SpCas9 PEmax prime editor

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   PEmax
   

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Detailed in manuscript

   Promoter

   CMV

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. c-Jun

   Plasmid

   #187902

   Purpose

   Expresses c-Jun in transfected cells

   Depositor

   Jennifer Mitchell

   Article

   Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

   Insert

   c-Jun (JUN Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-28b-Cas9-His

   Plasmid

   #47327

   Purpose

   For in vitro expression and purification of Cas9 protein

   Depositor

   Alex Schier

   Article

   Gagnon et al PLoS One. 2014 May 29;9(5):e98186. doi:

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   His

   Expression

   Bacterial

   Available Since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. mScarletI3-6DG5-MAPPER

   Plasmid

   #222421

   Purpose

   To express a label of ER-plasma membrane contacts based on GFP-MAPPER (#117721), but with mScarletI3 tag and replacing FRB with a protein of similar structure and sequence length (Neoleukin-2/15)

   Depositor

   Stephen Royle

   Article

   Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

   Insert

   MAPPER
   

   Tags

   mScarletI3-6DG5

   Expression

   Mammalian

   Available Since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCW57-CMV-ssRFP-GFP-KDEL

   Plasmid

   #128257

   Purpose

   Fluorescence reporter to monitor ER-phagy activity

   Depositor

   Noboru Mizushima

   Article

   Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

   Insert

   ssRFPGFPKDEL
   

   Use

   Lentiviral

   Tags

   RFPGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-tetON-NKX2-1-EF1a-TagRFP-2A-tet3G

   Plasmid

   #167942

   Purpose

   Inducible NKX2-1 overexpression

   Depositor

   Emma Rawlins

   Article

   Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

   Insert

   TTF1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Promoter

   tetON

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCherry-DFCP1

    Plasmid

    #86746

    Purpose

    N-terminal mCherry-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

    Insert

    human DFCP1 (ZFYVE1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. TFORF1759

    Plasmid

    #143845

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ALYREF (ALYREF Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZac2.1-GfaABC1D-mCherry-hPMCA2w/b

    Plasmid

    #111568

    Purpose

    Expresses a plasma membrane calcium pump hPMCA2w/b

    Depositor

    Baljit Khakh

    Article

    Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

    Has Service

    AAV5

    Insert

    Plasma membrane calcium pump 2, variant w/b (ATP2B2 Human)
    

    Use

    AAV

    Tags

    mCherry

    Available Since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. 35S:RUBY

    Plasmid

    #160908

    Purpose

    RUBY under the control of CaMV 35S promoter/marker for transformation

    Depositor

    Yunde Zhao

    Article

    He et al Hortic Res. 2020 Sep 19;7:152. doi: 10.1

    Insert

    RUBY and CaMV 35S promoter
    

    Expression

    Plant

    Promoter

    CaMV 35S

    Available Since

    Nov. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV delta R8.2

    Plasmid

    #12263

    Purpose

    First-generation packaging plasmid. This plasmid contains HIV accessory genes. Please reach out to your biosafety officer for guidance.

    Depositor

    Didier Trono

    Insert

    HIV-1 GAG/POL, Tat and Rev
    

    Use

    Lentiviral; Packaging

    Expression

    Mammalian

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. synIRES_RC25

    Plasmid

    #189970

    Purpose

    Part 2 plasmid to introduce the HRV-B3 IRES with proximal loop Apt-eIF4G into circRNAs

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    synIRES_RC25
    

    Use

    Cloning

    Promoter

    N/A

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBBR-Cas9ΔyqhD-HA500

    Plasmid

    #220369

    Purpose

    A single-plasmid-based, easily cured CRISPR/Cas9 system for genome engineering in Pseudomonas putida KT2440

    Depositor

    Yilan Liu

    Article

    Wen et al Microb Cell Fact. 2024 Dec 30;23(1):349.

    Inserts

    SpCas9
    sgRNA for yqhD in P. putida KT2440
    

    Use

    CRISPR; Broad host range

    Expression

    Bacterial

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. SpyCatcher003-sfGFP

    Plasmid

    #133449

    Purpose

    Expresses SpyCatcher003-superfolder GFP in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyCatcher003-sfGFP
    

    Tags

    His6 and TEV site

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. PresentER-SIINFEKL (mCherry)

    Plasmid

    #102945

    Purpose

    PresentER minigene encoding H2-Kb ligand SIINFEKL (mCherry)

    Depositor

    David Scheinberg

    Article

    Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

    Insert

    SIINFEKL (OVAL Synthetic, Chicken)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    OVA257-264

    Promoter

    LTR

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

19. pDG459

    Plasmid

    #100901

    Purpose

    SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG and T2A-PuroR

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHL-sec

    Plasmid

    #99845

    Purpose

    Mammalian expression with secretion signal sequence

    Depositor

    Edith Yvonne Jones

    Article

    Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

    Insert

    ephrin b2 (EFNB2 Human)
    

    Tags

    His

    Expression

    Mammalian

    Available Since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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