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Plasmid#172616PurposeExpresses 2xFLAG-2xSTREP-tagged CDK2 in mammalian cellsDepositorInsertCDK2 (CDK2 Human)
UseTags2xFLAG-2xSTREPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
betaTrCP mVenus mutant reporter
Plasmid#192459PurposeFluorescent reporter for beta-TrCP with a non-degradable mutation. Used as a control.DepositorInsertCdC25B
UseLentiviralTagsSV40 NLS and mVenusExpressionMammalianMutationaa198-338 of CDC25B. betaTrCP degron sequence (aa…PromoterEF1alphaAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
SOX2 TALE Repressor (KRAB 11-75)
Plasmid#42946DepositorInsertSOX2 TALE Repressor (KRAB 11-75)
UseLentiviralTags2A-GFPExpressionMammalianMutationPromoterEF-1aAvailable sinceFeb. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSIN ReP5-GFP-GluR1
Plasmid#24004DepositorInsertGluR1 (Gria1 Rat)
UseSindbis virus productionTagsGFPExpressionMutationPromoterAvailable sinceApril 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
6xHis(TEV)-dDrp1-StrepII
Plasmid#174421PurposeExpresses D. melanogaster Drp1 in bacteriaDepositorInsertDrp1 (Drp1 Fly)
UseTags6xHis(TEV) and StrepIIExpressionBacterialMutationNonePromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Gm-REP
Plasmid#64966Purposemini-Tn7 vector with oriR6K, for attTn7 site determinationDepositorInsertoriR6K
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMS17: pTwinStrep-SUMO-AvTnsD
Plasmid#168150PurposeAvTnsD expression construct for protein purification.DepositorInsertAvCAST TnsD
UseTags6X His, Twin Strep and SUMOExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS15: pTwinStrep-SUMO-AvTnsB
Plasmid#168148PurposeAvTnsB expression construct for protein purification.DepositorInsertAvCAST TnsB
UseTags6X His, Twin Strep and SUMOExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS16: pTwinStrep-SUMO-AvTnsC
Plasmid#168149PurposeAvTnsC expression construct for protein purification.DepositorInsertAvCAST TnsC
UseTags6X His, Twin Strep and SUMOExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS14: pTwinStrep-SUMO-AvTnsA
Plasmid#168147PurposeAvTnsA expression construct for protein purification.DepositorInsertAvCAST TnsA
UseTags6X His, Twin Strep and SUMOExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
eGFP Y93H GC editing reporter
Plasmid#198884PurposeExpress eGFP fluorescence after Y93H correction and mCherry as transfection controlDepositorInsertenhanced green fluorescent protein
UseLentiviralTagsExpressionMammalianMutationY93H, Gly92(GGT-to-GGG)PromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
PX385 Streptococcus pasteurianus Cas9
Plasmid#68322PurposeStreptococcus pasteurianus Cas9DepositorInsertSpaCas9
UseCRISPRTagsHA and NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOET3-N-TEV-Twin-Strep
Plasmid#203323PurposeGenerate the baculovirus to express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in insect cellsDepositorTypeEmpty backboneUseTagsTEV cleavable site (ENLYFQG) and Twin-strep-tagExpressionInsectMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lepr peak reporter-eGFP
Plasmid#196584PurposeCis-regulatory region of mouse Lepr gene driven a eGFP reporter with hGFP driven rtTA3DepositorInserteGFP
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterSV40Available sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMS32: pTwinStrep-SUMO-PmcTnsC
Plasmid#168165PurposePmcTnsC expression construct for protein purification.DepositorInsertPmcCAST TnsC
UseTags6X His, Twin Strep and SUMOExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
T7 promoter + rpsL reporter plasmid
Plasmid#156455PurposeA mutational reporter plasmid that can gain Streptomycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.DepositorInsertsrpsL
TcR
UseTagsExpressionBacterialMutationATG start codon mutated to ACGPromotertac and tetAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDFNKI-his-3C-LIC-strep
Plasmid#108705PurposeExpression of a His-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneUseTagsHis-3C protease cleavage siteExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-2xFLAG-2xSTREP-CDK4
Plasmid#172615PurposeExpresses 2xFLAG-2xSTREP-tagged CDK4 in mammalian cellsDepositorInsertCDK4 (CDK4 Human)
UseTags2xFLAG-2xSTREPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS31: pTwinStrep-SUMO-PmcTnsAB
Plasmid#168164PurposePmcTnsAB expression construct for protein purification.DepositorInsertPmcCAST TnsAB
UseTags6X His, Twin Strep and SUMOExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAC8-TwinStrep-3C-DrHDAC6 (40-831)
Plasmid#180226PurposeBaculovirus transfer plasmid to express TwinStrep-3C-DrHDAC6 (40-831)DepositorInsertHistone deacetylase 6 (hdac6 Zebrafish)
UseTagsTwinStrep-3CExpressionInsectMutationPromoterpolyhedrinAvailable sinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMS33: pTwinStrep-SUMO-PmcTnsD
Plasmid#168166PurposePmcTnsD expression construct for protein purification.DepositorInsertPmcCAST TnsD
UseTags6X His, Twin Strep and SUMOExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET51b-Strep-hAGT-His
Plasmid#167276PurposeOverexpression of the human o_-alkylguanine-dna alkyltransferase in E. coliDepositorInserthAGT (AGT Human)
UseTagsHis x10 and Strep-tagExpressionBacterialMutationPromoterT7Available sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXP420 v2 (repaired Amp promoter)
Plasmid#86920PurposeShuttle vector to facilitate gene expression for metabolic engineering in S. cerevisiae. Contains TEF1 promoter and CYC1 terminator for insertion of gene of interest. Contains HIS3 selection marker.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterTEF1Available sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-2xFLAG-2xSTREP-CCND3
Plasmid#172623PurposeExpresses 2xFLAG-2xSTREP-tagged cyclin D3 in mammalian cellsDepositorInsertCCND3 (CCND3 Human)
UseTags2xFLAG-2xSTREPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(EF1a)-TTN
Plasmid#172327PurposeLV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an EF1a promoter driving nuclear d2eGFP E2A HygromycinDepositorInsertsmonomeric nuclear Tomato
d2eGFP
HygR
UseLentiviral and Synthetic BiologyTags3x SV40 NLSExpressionMammalianMutationPromoterEF1-alphaAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETNKI-strepII-3C-LIC-kan
Plasmid#108706PurposeExpression of a StrepII-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneUseTagsStrepII-3C protease cleavage siteExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-2xFLAG-2xSTREP-CCND2
Plasmid#172629PurposeExpresses 2xFLAG-2xSTREP-tagged cyclin D2 in mammalian cellsDepositorInsertCCND2 (CCND2 Human)
UseTags2xFLAG-2xSTREPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-Strep-TEV-Nsp14
Plasmid#154955PurposeExpresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable Strep tag in E. coliDepositorInsertStrep-TEV-Nsp14 (ORF1ab SARS-CoV-2)
UseTagsStrep-TEVExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUPD2:35S-TMV-Rep-MP
Plasmid#106217PurposeProvides CaMV 35S and 5' part of Tobacco mosaic virus (RdRp polymerase with intron, movement protein and sgCP promoter) as level 0 GoldenBraid part (GGAG-AATG)DepositorInsertInfectious clone of Tobacco mosaic virus, 5' part
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b-Strep-SsOGT-H5-His
Plasmid#167277PurposeOverexpression of a sulfolobal equivalent of SNAP-tag in E. coliDepositorInsertSsOGT-H5
UseTagsHis x10 and Strep-tagExpressionBacterialMutationPromoterT7Available sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only