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Showing: 1201 - 1250 of 1933 results

  10. pJET-63-SFFV-T2A-BlaR-polyA-R11a

    Plasmid
    #179894
    Purpose
    Donor with homologous arms for Rab11a to knock in SFFV-T2A-Blasticidin resistance-PolyA cassette (for knock out)
    Depositor
    Patrick Caswell
    Article
    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:
    Insert
    RAB11A, member RAS oncogene family (RAB11A Human)
    Use
    CRISPR; Donor for homologous based knock in
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    SFFV
    Available since
    July 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    179894-plasmid-map-sequence-id-366023

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  11. pJET-42-DExCon-antiGFPnanobody-mCherry-R11a

    Plasmid
    #179902
    Purpose
    Donor with homologous arms for Rab11a to knock in DExCon-antiGFPnanobody-mCherry module
    Depositor
    Patrick Caswell
    Article
    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:
    Insert
    RAB11A, member RAS oncogene family (RAB11A Human)
    Use
    CRISPR; Donor for homologous based knock in
    Tags
    antiGFPnanobody-mCherry
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    TRE3GS
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    179902-plasmid-map-sequence-id-357194

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  12. pJET-61-SFFV-T2A-PuroR-polyA-R11a

    Plasmid
    #179892
    Purpose
    Donor with homologous arms for Rab11a to knock in SFFV-T2A-Puromycin resistance-PolyA cassette (for knock out
    Depositor
    Patrick Caswell
    Article
    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:
    Insert
    RAB11A, member RAS oncogene family (RAB11A Human)
    Use
    CRISPR; Donor for homologous based knock in
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    SFFV
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    179892-plasmid-map-sequence-id-358130

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  14. pcDNA3.1+FLAG-KrascomG12D

    Plasmid
    #206844
    Purpose
    To express mouse Kras encoded by common mouse codons (to increase expression) and with a G12D mutation with an N-terminal FLAG tag.
    Depositor
    Christopher Counter
    Article
    Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755
    Insert
    Kras (Kras Mouse)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Codons altered to most optimum based on mouse cod…
    Promoter
    Available since
    Oct. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    206844-plasmid-map-sequence-id-409574

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  16. pSLPB3B-AID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1

    Plasmid
    #187960
    Purpose
    AID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Insert
    AID-SpdCas9-tagRFPt-P2A-tagBFP; Blasticidin-T2A-osTIR1
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187960-plasmid-map-sequence-id-373524

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  18. pSLQ2818_Blasti_pPB: CAG-PYL1-KRAB-IRES-Blasti-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9-FKBP_F36V

    Plasmid
    #187959
    Purpose
    Expressed ABA-inducible dimerizing KRAB-dCas9 system, with KRAB-IRES-Blasticidin resistance under CAG promoter and tagBFP-dCas9-FKBP12 (F36V mutant) degron under PGK promoter in a piggyBac plasmid.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Inserts
    Blasticidin resistance
    FKBP12_F36V
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187959-plasmid-map-sequence-id-373708

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  19. pSLPB3B-mAID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1

    Plasmid
    #187963
    Purpose
    mAID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Inserts
    AID-SpdCas9-tagRFPt-P2A-tagBFP; Blasticidin-T2A-osTIR1
    osTIR1
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker and T2A
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187963-plasmid-map-sequence-id-373709

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  21. pAav-TP53-T2A-BirA*

    Plasmid
    #115652
    Purpose
    rAAV-based donor template for genome engineering of the TP53 protein C-terminus containing a T2A-BirA* module and a selection cassette
    Depositor
    Sven Eyckerman
    Article
    Vandemoortele et al J Proteome Res. 2018 Dec 10. doi: 10.102
    Inserts
    TP53 (TP53 Human)
    TP53 (TP53 Human)
    T2A
    BirA*
    Use
    AAV
    Tags
    BirA* and T2A
    Expression
    Mutation
    Homology region 1 and Homology region 2
    Promoter
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    115652-plasmid-map-sequence-id-223051

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  22. KI(GAPDH)-P2A-EGFP-CMV-NeoR

    Plasmid
    #114009
    Purpose
    HDR-cassette to target chicken GAPDH gene for EGFP expression under control of the endogenous promoter. The cassette contains NeoR gene for positive clone selection and DTA gene for negative selection
    Depositor
    Pavel Volchkov
    Article
    Antonova et al F1000Res. 2018 Feb 28;7:238. doi: 10.126
    Inserts
    GAPDH left arm (GAPDH Chicken)
    eGFP
    NeoR
    GAPDH right arm
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    -, CMV, and endogenous promoter (chicken GAPDH in…
    Available since
    Jan. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    114009-plasmid-map-sequence-id-261238

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  24. pcDNA3.1+FLAG-KrascomQ61R

    Plasmid
    #206845
    Purpose
    To express mouse Kras encoded by common mouse codons (to increase expression) and with a Q61R mutation with an N-terminal FLAG tag.
    Depositor
    Christopher Counter
    Article
    Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755
    Insert
    Kras (Kras Mouse)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Codons altered to most optimum based on mouse cod…
    Promoter
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    206845-plasmid-map-sequence-id-441039

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  26. pCF806-shABC2

    Plasmid
    #186714
    Purpose
    Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC2 = shARAF.169-shBRAF.1296-shCRAF.387). The shABC2 set is better than shABC1.
    Depositor
    Christof Fellmann
    Article
    Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023
    Insert
    ARAF, BRAF, CRAF (BRAF, RAF1, ARAF Human)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    186714-plasmid-map-sequence-id-382420

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  27. pCF806-shABC1

    Plasmid
    #186713
    Purpose
    Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC1 = shARAF.381-shBRAF.2015-shCRAF.2446). The shABC2 set is better than shABC1.
    Depositor
    Christof Fellmann
    Article
    Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023
    Insert
    ARAF, BRAF, CRAF (BRAF, RAF1, ARAF Human)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    186713-plasmid-map-sequence-id-376087

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  29. makeTCR_HDR_Hs.TRAC_Backbone

    Plasmid
    #233944
    Purpose
    backbone for makeTCR assembly
    Depositor
    Michael Platten
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    knock-in to endogenous TRAC
    Available since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    233944-plasmid-map-sequence-id-470000

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  30. pRY003

    Plasmid
    #81043
    Purpose
    HO endonuclease expression
    Depositor
    John McCusker
    Insert
    HO
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    endogenous
    Available since
    Nov. 8, 2016
    Availability
    Academic Institutions and Nonprofits only

  31. pUC19_GZG

    Plasmid
    #117226
    Purpose
    shble flanked by an endogenous GAPDH promoter and terminator system
    Depositor
    Jackie Collier
    Insert
    shble
    Use
    Thraustochytrid expression
    Tags
    Expression
    Mutation
    Promoter
    GAPDH
    Available since
    Oct. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    117226-plasmid-map-sequence-id-224760

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  33. hSLBP_mNG_FS_Donor

    Plasmid
    #191387
    Purpose
    Donor for C-terminal tagging of endogenous hSLBP
    Depositor
    Yannick Doyon
    Insert
    mNeonGreen(mNG) + FS(3xFLAG_TwinStrep)Tag for hSLBP tagging at the C-terminus (SLBP Synthetic, Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191387-plasmid-map-sequence-id-378113

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  39. pLinker-6xHA-HXGPRT-LoxP

    Plasmid
    #86552
    Purpose
    The plasmid is used for endogenous tagging using CRISPR technology in Toxoplasma
    Depositor
    David Sibley
    Insert
    Linker-6xHA-HGPRT-LoxP
    Use
    Expression in toxoplasma gondii
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    86552-plasmid-map-sequence-id-190138

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  40. HC080

    Plasmid
    #59545
    Purpose
    Expresses yeast Atg13 under the endogenous promoter with a GFP N-terminal tag
    Depositor
    Daniel Klionsky
    Insert
    ATG13 (ATG13 Budding Yeast)
    Use
    Tags
    GFP
    Expression
    Bacterial and Yeast
    Mutation
    AvrII site created after start codon
    Promoter
    ATG13
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  42. AAVS1-SAGFP

    Plasmid
    #127100
    Purpose
    SA-GFP substrate (Addgene #41594) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. SA-GFP cassette contains PGK-puromycin.
    Depositor
    Adam Karpf
    Insert
    SA-GFP reporter
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    127100-plasmid-map-sequence-id-247555

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  43. GBH-R-cmlc2GFP.1

    Plasmid
    #188004
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted mRFP protein trap; secondary marker cmlc2 GFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; cmlc2 promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188004-plasmid-map-sequence-id-381042

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  44. GBH-R-gcryGFP.3

    Plasmid
    #188000
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted mRFP protein trap; secondary marker gamma crystallin GFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; gamma crystallin promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188000-plasmid-map-sequence-id-381121

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  45. GBH-2aG4-insBFP.1

    Plasmid
    #188025
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted 2aGal4VP16 protein trap; secondary marker insulin BFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; insulin promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188025-plasmid-map-sequence-id-381128

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  46. GBH-2aG4-insGFP.1

    Plasmid
    #188028
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted 2aGal4VP16 protein trap; secondary marker insulin GFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; insulin promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188028-plasmid-map-sequence-id-381113

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  47. GBH-2aG4-insGFP.2

    Plasmid
    #188029
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted 2aGal4VP16 protein trap; secondary marker insulin GFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; insulin promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188029-plasmid-map-sequence-id-381142

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  48. GBH-R-insBFP.2

    Plasmid
    #188008
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted mRFP protein trap; secondary marker insulin BFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; insulin promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188008-plasmid-map-sequence-id-381105

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  49. GBH-R-insBFP.3

    Plasmid
    #188009
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted mRFP protein trap; secondary marker insulin BFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; insulin promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188009-plasmid-map-sequence-id-381106

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  50. GBH-R-insGFP.1

    Plasmid
    #188010
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted mRFP protein trap; secondary marker insulin GFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; insulin promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188010-plasmid-map-sequence-id-381122

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Showing: 1201 - 1250 of 1933 results