We narrowed to 13,664 results for: sequence

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pENTR-PKAKTRClover

Plasmid

#59146

Purpose

ORF encoding PKA KTR mClover flanked by gateway sequences

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

PKA Kinase Translocation Reporter (PRKACA Human, Mouse)

Available Since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pAL204

Plasmid

#22282

Purpose

Expresses PhyB-mCFP with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membrane

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

pCMV-PhyB(1-908)-10aaLinker-mCFP-Kras4BCT (PHYB Mustard Weed)

Use

Synthetic Biology

Expression

Mammalian

Available Since

Feb. 19, 2010

Availability

Academic Institutions and Nonprofits only

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pNP1 eforRed trigger

Plasmid

#107357

Purpose

Trigger sequence for pNP1 eforRed toehold switch

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

toehold trigger 1

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pHBS1500 [IBB-GFP-mCherry3E]-[BFP-TDP43 deltaNTD]

Plasmid

#133324

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

Deletion of NTD in full-length TDP43

Available Since

Nov. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLP16_Lenti Scramble Control

Plasmid

#239417

Purpose

Negative control Lentiviral plasmid for SpCas9-based CRISPR KO

Depositor

Subhojit Roy

Article

Parra-Rivas et al STAR Protoc. 2025 Jul 21;6(3):103945. do

Insert

Scramble sequence

Use

Lentiviral

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SERA9-bio-His

Plasmid

#50820

Purpose

Expresses enzymatically monobiotinylated full length SERA9

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

SERA9

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 12, 2014

Availability

Academic Institutions and Nonprofits only

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pDL0_158

Plasmid

#210779

Purpose

PUSCT_Drosophila melanogaster Gypsy-insulator sequence

Depositor

Dmitri Nusinow

Article

Lee et al Plant Biotechnol J. 2025 Dec 5. doi: 10.

Insert

Gypsy insulator

Use

Synthetic Biology

Expression

Plant

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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YFP-HA-DAT

Plasmid

#90244

Purpose

Expresses YFP-HA-DAT in mammalian cells

Depositor

Alexander Sorkin

Article

Sorkina et al J Neurosci. 2006 Aug 2;26(31):8195-205.

Insert

DAT (dopamine transporter) (SLC6A3 Human)

Expression

Mammalian

Mutation

residues 193–203 of human DAT were replaced in YF…

Promoter

CMV

Available Since

June 15, 2017

Availability

Academic Institutions and Nonprofits only

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MSRP4-bio-His

Plasmid

#50806

Purpose

Expresses enzymatically monobiotinylated full length MSRP4

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

MSRP4

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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PFA0210c-bio-His

Plasmid

#50821

Purpose

Expresses enzymatically monobiotinylated full length PFA0210c

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

PFA0210c

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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Ubiq-Cas9.874W

Plasmid

#112686

Purpose

Express hSpCas9 under Ubiquitin-63E promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

hSpCas9

Tags

3x FLAG, EGFP, and NLS

Expression

Insect

Mutation

Humanized Cas9 bacterial sequence

Promoter

Ubiquitin-63E promoter

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_Cerulean_Nanotag_70_neg_Control

Plasmid

#130568

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

negative oligo sequence

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCRIS

Plasmid

#120424

Purpose

This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3, IS5, and IS150.

Depositor

Tamás Fehér

Article

Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:

Insert

CRISPR spacers targeting IS1, IS5, IS3, IS150

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

constitutive

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pMCS-rybozyme-IRES-CAS9

Plasmid

#64668

Purpose

Plasmid encoding multiple cloning site, rybozyme and IRES-CAS9.

Depositor

Wataru Fujii

Article

Yoshioka et al Sci Rep. 2015 Dec 16;5:18341. doi: 10.10

Inserts

CAS9
HDV ribozyme

Use

CRISPR

Tags

Flag, Internal ribosome entry site, and guide RNA…

Expression

Mammalian

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCEP4-DU422gp160

Plasmid

#100926

Purpose

Mammalian expression plasmid for Env from the DU422.1 HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (DU422.1) Env

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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EK0441 CMV-TO-BFPmut-stop-EGFP.t2 (FLP-IN)

Plasmid

#191161

Purpose

Expression of a trigger sequence from EGFP CDS in 3' UTR context in mammalian cells

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

EGFP.t2

Expression

Mammalian

Mutation

WT

Promoter

CMV-TO

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pPagP

Plasmid

#112125

Purpose

Bacterial expression of outer membrane protein PagP, wild type

Depositor

Karen Fleming

Article

Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

Insert

PagP (pagP E. coli)

Expression

Bacterial

Mutation

N-terminal signal sequence not included and repla…

Promoter

T7

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pT3TS_Transposase

Plasmid

#109768

Purpose

Codon optimised transposase sequence in an optimal vector for in vitro transcription. Contains the T3 promoter and synthetic polyA tract.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Tol2 Transposase

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-C2gamma

Plasmid

#21215

Depositor

Tobias Meyer

Article

Codazzi et al Curr Biol. 2001 Jul 24. 11(14):1089-97.

Insert

GFP-pcDNA3-PKCgamma-c2 (Prkcg Rat)

Tags

GFP

Expression

Mammalian

Mutation

The C2-domain from PKCgamma (aa170-260) plus flan…

Available Since

July 13, 2009

Availability

Academic Institutions and Nonprofits only

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pCEP4-25711gp160

Plasmid

#111841

Purpose

Mammalian expression plasmid for Env from the 25711 HIV-1 strain

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (25711.2) Env

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pJK2020

Plasmid

#171932

Purpose

express MS2 coat protein (MCP) fused to superfolder GFP and histone subunit H2B fused to mCherry in an operon

Depositor

Judith Kimble

Article

Lee et al Dev Cell. 2019 Aug 19;50(4):426-435.e4.

Insert

Pmex-5::MS2 Coat Protein::linker::sfGFP::tbb-2 3'UTR::gpd-2 intergenic sequence::H2B::mCherry::unc-54 3'UTR

Tags

superfolder GFP, mCherry

Expression

Worm

Promoter

-

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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EC2_3_dCas9_Mxi1_sgRNA

Plasmid

#163708

Purpose

Yeast low copy plasmid with dCas9, sgRNA expression cassette and Mxi1 transcriptional repressor

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Inserts

dCas9
Mxi1+SV40 NLS

Expression

Yeast

Mutation

Point mutations D10A and H840A

Promoter

ScTEF1

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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V2-MESA-35F-M-dCas9

Plasmid

#84506

Purpose

MESA target chain with V2-MESA ectodomain, 35 extracellular linkers, a flag tag, M cleavage sequence, and dCas9-VP64

Depositor

Joshua Leonard

Article

Schwarz et al Nat Chem Biol. 2016 Dec 12. doi: 10.1038

Insert

V2-MESA-35F-M-dCas9

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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JH02

Plasmid

#73511

Purpose

mCherryo_PH pCFJ150 - contians C. elegans sequence optimized mCherry

Depositor

Bob Goldstein

Article

Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

Insert

pmex-5::mCherryo::PH::tbb-2

Tags

mCherry

Promoter

pmex-5

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pTAMAHISTEV_BauA

Plasmid

#128952

Purpose

expresses BauA in E.coli

Depositor

James Naismith

Article

Moynie et al Elife. 2018 Dec 18;7. pii: 42270. doi: 1

Insert

ferric acinetobactin receptor

Tags

TEV protease cleavable 7xHis and TamA

Expression

Bacterial

Mutation

Signal peptide sequence has been removed (1-66)

Promoter

T7

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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PEX-LV

Plasmid

#12120

Depositor

Inder Verma

Article

Pfeifer et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

Insert

Matrix Metalloproteinase 2 (Mmp2 Mouse)

Use

Lentiviral

Tags

FLAG

Mutation

noncatalytic fragment of MMP2 comprised of the fi…

Available Since

April 27, 2007

Availability

Academic Institutions and Nonprofits only
ii-Str_SBP-mCherry-GPI

Plasmid

#65297

Purpose

synchronize trafficking of mCherry-GPI from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

GPI anchor

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-FLEx-TetOn3G-3xHA-FLEx

Plasmid

#133729

Purpose

Plasmid contains the floxed TetOn3G-transactivator sequence for expression of TetOn3G in the presence of Cre recombinase.

Depositor

Erin Schuman

Article

Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

Insert

TetOn3G-3xHA (floxed & inverted)

Use

AAV

Tags

3xHA-tag

Expression

Mammalian

Promoter

pCMV

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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tdTomato-β-actinCDS3

Plasmid

#183715

Purpose

tdTomato with fragment nucleotides 801-1127 of beta-actin coding sequence inserted in 3'UTR

Depositor

Jeffrey Gerst

Article

Dasgupta et al Elife. 2023 May 30;12:e83584. doi: 10.75

Insert

tdTomato with fragment (nucleotides 801-1127) of mouse Actb coding dsequence

Use

Lentiviral

Promoter

UbiC

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC22A1

Plasmid

#132034

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

Insert

SLC22A1 (SLC22A1 Human)

Expression

Mammalian

Available Since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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