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164,223 results

Showing: 9961 - 10000 of 164223 results

  1. HDM_SARS2_Spike_del21_D614G

    Plasmid
    #158762
    Purpose
    Mammalian expression vector for expressing SARS-2 Spike with 21 aa del at C-terminus and pt mutation D to G at site 614
    Depositor
    Jesse Bloom
    Article
    Crawford et al Viruses. 2020 May 6;12(5). pii: v1205051
    Insert
    SARS2-Spike_del21_D614G (S Synthetic, Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    deleted amino acids 1257-1278, changed aspartic a…
    Promoter
    CMV
    Available Since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158762-plasmid-map-sequence-id-309495

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  4. pHAGE2-TetOminiCMV-Oct4-p2a-Sox2-17-t2a-Klf4-e2a-cMyc

    Plasmid
    #193346
    Purpose
    tetO-OS*KM (Dox-inducible reprogramming lentiviral vector expressing mouse Oct4 + super-Sox + Klf4 + cMyc)
    Depositor
    Hans Schöler
    Article
    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147
    Insert
    Oct4-p2a-Sox2-17-t2a-Klf4-e2a-cMyc (Klf4, Pou5f1, Sox2 Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Sox2-17 is engineered highly cooperative chimeric…
    Promoter
    TetO mini CMV
    Available Since
    Feb. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    193346-plasmid-map-sequence-id-384485

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  8. pX330-BsaIx2-DD-Cas9

    Plasmid
    #75380
    Purpose
    Expresses DD-SpCas9 in mammalian cells and has a cloning site for an sgRNA. The FKBP12 L106P destabilization domain allows for inducible stabilization of Cas9 through the addition of Shield-1
    Depositor
    Michele Calos
    Article
    Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv
    Insert
    DD-SpCas9
    Use
    CRISPR
    Tags
    FKBP12 L106P DD
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available Since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    75380-plasmid-map-sequence-id-192102

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  13. pBUN411

    Plasmid
    #50581
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    3×FLAG-NLS-zCas9-NLS
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG and NLS
    Expression
    Mutation
    maize codon optimized
    Promoter
    OsU3p and Ubi1p
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50581-plasmid-map-sequence-id-311045

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  14. TFORF1167

    Plasmid
    #142744
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    EBF3 (EBF3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142744-plasmid-map-sequence-id-395819

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  15. pINDUCER20_3xFlag

    Plasmid
    #190028
    Purpose
    Doxycycline-inducible lentiviral vector for expression of ORF with C-terminal 3xFlag
    Depositor
    Andrew Tutt
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Nov. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    190028-plasmid-map-sequence-id-377387

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  18. pcDNA3-endo-GsCT

    Plasmid
    #205516
    Purpose
    Endosome-targeted C-terminal fragment of human Gαs protein (Gαs inhibitory peptide).
    Depositor
    Jin Zhang
    Article
    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:
    Insert
    endo-GsCT (GNAS Human, Synthetic)
    Use
    Tags
    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Oct. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    205516-plasmid-map-sequence-id-409476

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  23. A24M04 mIgM heavy chain-pFEEKW

    Plasmid
    #234809
    Purpose
    Express membrane form of IgM heavy chain containing the VH region of the monoclonal antibody, A24M04, specific for human papilloma virus16 L1
    Depositor
    Susan Pierce
    Article
    Torgbor et al Proc Natl Acad Sci U S A. 2025 Mar 11;12
    Insert
    A24M04 membrane IgM heavy chain (IGHM Human)
    Use
    Lentiviral
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    EEK promoter
    Available Since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    234809-plasmid-map-sequence-id-466813

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  29. Pet28a_Human_H4

    Plasmid
    #42633
    Depositor
    Joe Landry
    Insert
    Human H4 (H4C1 Human)
    Use
    Tags
    Untagged
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Feb. 25, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    42633-plasmid-map-sequence-id-57748

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  35. pCAMBgfp

    Plasmid
    #206130
    Purpose
    This binary vector has been generated for introduction of SGFP in filamentous fungi using ATMT. Contains the hygromycin resistance gene and the SGFP gene.
    Depositor
    Anne Osbourn
    Article
    Sesma et al Nature. 2004 Sep 30;431(7008):582-6. doi
    Insert
    hygromycin resistance gene and the Promoter of ToxA+SGFP
    Use
    Binary vector for expression of sgfp in filamento…
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    206130-plasmid-map-sequence-id-417184

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  36. CMV-R-GECO1

    Plasmid
    #32444
    Purpose
    Mammalian expression of Red fluorescent genetically encoded Ca2+ indicator for optical imaging
    Depositor
    Robert Campbell
    Article
    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.
    Insert
    R-GECO1.0
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Substitutions relative to the mApple-derived anal…
    Promoter
    CMV
    Available Since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only
    32444-plasmid-map-sequence-id-117093

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  39. pBabe-Puro-BRAF-V600E

    Plasmid
    #15269
    Purpose
    Retroviral expression of BRAF V600E (the V600E mutation has been found in cancer patients, confers increased kinase activity, and is transforming)
    Depositor
    William Hahn
    Article
    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.
    Insert
    BRAF (BRAF Human)
    Use
    Retroviral
    Tags
    His and Myc
    Expression
    Mammalian
    Mutation
    Valine 600 to Glutamate (mutation has been found …
    Promoter
    Available Since
    July 12, 2007
    Availability
    Academic Institutions and Nonprofits only
    15269-plasmid-map-sequence-id-217979

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Showing: 9961 - 10000 of 164223 results