159,258 results

1. p31sheLL

   Plasmid

   #37322

   Depositor

   John Schiller

   Article

   Roberts et al Nat Med. 2007 Jul;13(7):857-61. Epub 200

   Insert

   HPV31L1+L2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJAM1.19_PB-CIT-PGK-Blast

   Plasmid

   #205564

   Purpose

   Express human CIT

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   CIT (CIT Human)
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-REPORT(PGK/CMV)

   Plasmid

   #172330

   Purpose

   LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an PGK/CMV promoter driving nuclear d2eGFP E2A Hygromycin

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Inserts

   monomeric nuclear Tomato
   d2eGFP
   HygR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3x SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   PGK/CMV

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-BAF

   Plasmid

   #227442

   Purpose

   Mammalian expression of GFP-BAF

   Depositor

   David Pellman

   Article

   Umbreit et al Science. 2020 Apr 17;368(6488):eaba0712.

   Insert

   Barrier-to-autointegration factor (BANF1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-FLAG-mISG15

   Plasmid

   #12443

   Depositor

   Dong-Er Zhang

   Article

   Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

   Insert

   ISG15
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCrlM

   Plasmid

   #122444

   Purpose

   Expresses myc tagged mouse reelin ORF in mammalian cells

   Depositor

   Tom Curran

   Article

   D'Arcangelo et al J Neurosci. 1997 Jan 1;17(1):23-31.

   Insert

   reelin (Reln Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYLCRISPR/Cas9P35S-H

   Plasmid

   #66189

   Purpose

   expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), hygro selection

   Depositor

   Yao-Guang Liu

   Article

   Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Plant

   Mutation

   plant-codon optimized with higher GC contents (62…

   Promoter

   cauliflower mosaic virus 35S promoter (P35S)

   Available since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. LRP6-pCS2

   Plasmid

   #27242

   Depositor

   Xi He

   Article

   Tamai et al Nature. 2000 Sep 28. 407(6803):530-5.

   Insert

   low density lipoprotein receptor-related protein 6 (LRP6 Human)
   

   Use

   Xenopus injection

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDESTmycYBX1

   Plasmid

   #19878

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al RNA. 2008 Oct 31. ():.

   Insert

   Y box binding protein 1 (YBX1 Human)
   

   Use

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CD20

    Plasmid

    #1890

    Depositor

    Sander van den Heuvel

    Article

    van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

    Insert

    CD20 (MS4A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST exorh:mCherry

    Plasmid

    #195989

    Purpose

    Transgenesis backbone

    Depositor

    Christian Mosimann, Nicolas Rohner

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Type

    Empty backbone

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCherry-TrioN

    Plasmid

    #129621

    Purpose

    RhoGEF

    Depositor

    Dorus Gadella

    Article

    Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

    Insert

    N-terminal part of TRIO
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pESC-leu2d(empty)

    Plasmid

    #20120

    Depositor

    Jay Keasling

    Article

    Ro et al Nature. 2006 Apr 13. 440(7086):940-3.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. RCASBP-Y NHA-EGFP

    Plasmid

    #11481

    Depositor

    William Pavan

    Article

    Loftus et al DNA Res. 2001 Oct 31. 8(5):221-6.

    Insert

    Enhanced Green Fluoresecent Protein
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    March 22, 2006

    Availability

    Academic Institutions and Nonprofits only

15. HA-NEDD8

    Plasmid

    #18711

    Depositor

    Edward Yeh

    Article

    Kamitani et al J Biol Chem. 1997 Nov 7. 272(45):28557-6

    Insert

    NEDD8 (NEDD8 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG-Myr-p110-IH

    Plasmid

    #15689

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Nature. 2003 May 29. 423(6939):541-5.

    Insert

    p110 alpha (Pik3ca Mouse)
    

    Use

    Tags

    Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pTD-NTwinStrep_Km

    Plasmid

    #45938

    Purpose

    use to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Type

    Empty backbone

    Use

    Tags

    6xHis and Twin-Strep

    Expression

    Bacterial

    Mutation

    Promoter

    Trc (lactose/IPTG inducible)

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMVV102

    Plasmid

    #215308

    Purpose

    Expresses sfGFP under induction with the IPTG Marionette Cassette (LacIAM + PTac-sfGFP) with a BBR1 backbone.

    Depositor

    Brian Pfleger

    Article

    Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    PTac

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMVV103

    Plasmid

    #215309

    Purpose

    Expresses sfGFP under induction with the aTc Marionette Cassette (TetR + PTet-sfGFP) with a BBR1 backbone.

    Depositor

    Brian Pfleger

    Article

    Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CMV-FLEX-SaCas9-U6-sgDrd2

    Plasmid

    #159918

    Purpose

    Mutagenesis of Drd2

    Depositor

    Larry Zweifel

    Insert

    Drd2 (Drd2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDGB1_alpha1

    Plasmid

    #68224

    Purpose

    GoldenBraid level 1 Alpha plant expression vector.

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    empty vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pPB-R1R2_EF1ap300dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid

    #113343

    Purpose

    Expresses p300-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

    Depositor

    Gavin Wright

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    p300core-dCas9-VP64-T2A-MS2-p65-HSF1
    

    Use

    Piggybac

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TFORF0361

    Plasmid

    #142255

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TCEB3 (ELOA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pYES2/25Q

    Plasmid

    #1384

    Depositor

    Michael Sherman

    Article

    Meriin et al J Cell Biol 2002 Jun 10;157(6):997-1004.

    Insert

    huntingtin (25Q) (HTT Human)
    

    Use

    Tags

    EGFP and FLAG

    Expression

    Yeast

    Mutation

    Exon1 sequences containing the first 17 amino aci…

    Promoter

    Available since

    Feb. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. qTAG-N-Puro-dTAG

    Plasmid

    #207694

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-dTAG cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pHR-SFFV-dCas9-BFP

    Plasmid

    #46910

    Purpose

    Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS and tagBFP

    Depositor

    Jonathan Weissman, Stanley Qi

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Insert

    dCas9-BFP fusion
    

    Use

    CRISPR and Lentiviral

    Tags

    2xNLS, BFP, and HA

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLV.5’-LMNA-eGFP-KI

    Plasmid

    #170545

    Purpose

    Expresses a sgRNA for 5' tagging to LMNA and contains a cassette with eGFP flanked by homology arms for LMNA

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Inserts

    Left Homology arm for LMNA knockin
    Right Homology arm for VIM knockin
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pX330-FOXL2-cterm

    Plasmid

    #192893

    Purpose

    Expresses Cas9 and sgRNA targeting the C-terminus region of FOXL2

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    FOXL2 C-term sgRNA (FOXL2 Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMaCTag-Z31

    Plasmid

    #120074

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with V5

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    V5
    

    Use

    Pcr template

    Tags

    V5

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. human Alpha-synuclein

    Plasmid

    #51437

    Purpose

    human codon optimized Alpha-synuclein

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    Alpha-synuclein (SNCA Human)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 31, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pcDNA3.3_CoV2_D18

    Plasmid

    #170442

    Purpose

    Express SARS-CoV-2 WT spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 pseudotyped virus production.

    Depositor

    David Nemazee

    Article

    Rogers et al Science. 2020 Aug 21;369(6506):956-963.

    Insert

    SARS-CoV-2 spike D18 (S SARS-CoV-2, USA-WA1/2020 strain)
    

    Use

    Tags

    NA

    Expression

    Mammalian

    Mutation

    18aa deletion in c-terminal tail

    Promoter

    CMV

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3.1(-)-flag-UBR2

    Plasmid

    #50573

    Purpose

    Expresses FLAG-tagged mouse UBR2 in mammalian cells.

    Depositor

    Alexander Varshavsky

    Insert

    UBR2 (Ubr2 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

33. tre-stblHIF: pLenti-TRE-stblHIF-PGK-rtTAA-2A-Puro

    Plasmid

    #177203

    Purpose

    encoding stblHIF under inducible promoter

    Depositor

    Markus Covert

    Article

    Kudo et al bioRxiv (2022)

    Insert

    stblHIF
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    P402A/P577G/N813A

    Promoter

    TRE

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA HA HP1 alpha

    Plasmid

    #24078

    Depositor

    Naoko Tanese

    Article

    Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

    Insert

    HP1 alpha (CBX5 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

35. pLIB-TetR-KRAB-IRES-BLAS

    Plasmid

    #55820

    Purpose

    retroviral bicistronic expression vector for TetR-KRAB and Blasticidin S resistance

    Depositor

    Stephan Geley

    Article

    Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

    Insert

    TetR-KRAB
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCAG-VSVG

    Plasmid

    #35616

    Depositor

    Patrick Salmon, Arthur Nienhuis

    Insert

    VSV G protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLenti CMVTRE3G eGFP Puro (w819-1)

    Plasmid

    #27570

    Depositor

    Eric Campeau

    Insert

    Enhance Green Fluorescent Protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHAGE NFKB-TA-LUC-UBC-dTomato-W

    Plasmid

    #49335

    Purpose

    pHAGE NFKB-TA-LUC-UBC-dTomato-W

    Depositor

    Darrell Kotton

    Article

    Wilson et al Mol Ther. 2013 Apr;21(4):825-33. doi: 10

    Inserts

    NFkB consensus binding sequence
    TA
    Luciferase
    UBC
    dTomato
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBbS8c_ddCpf1_final

    Plasmid

    #212040

    Purpose

    Bacterial expression of the CRISPRi system for genome editing in E. coli

    Depositor

    Quanjiang Ji

    Article

    Zhang et al Nat Commun. 2024 Jan 27;15(1):825. doi:

    Insert

    ddCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    L-arabinose-inducible promoter PBAD

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3.1+_hsTRPA1_myc_dTom

    Plasmid

    #183179

    Purpose

    myc tagged hsTRPA1 used for cell transfection

    Depositor

    Sreekanth Chalasani

    Article

    Duque et al Nat Commun. 2022 Feb 9;13(1):600. doi: 1

    Inserts

    hsTRPA1 (TRPA1 Human)
    dTom
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge