We narrowed to 17,204 results for: puro

1. pLPCX-PECAMTS

   Plasmid

   #45850

   Depositor

   Martin Schwartz

   Article

   Conway et al Curr Biol. 2013 May 14. pii: S0960-9822(

   Insert

   PECAM1 (PECAM1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pD2529-CAG-Flag-mouse TGFb3

   Plasmid

   #180262

   Purpose

   Mouse full length TGFβ3 expression

   Depositor

   Timothy Springer

   Article

   Jiang et al Cancer Immunol Res. 2022 Feb 18. pii: 68

   Insert

   TGFβ3 (Tgfb3 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TRAIP-SFB

   Plasmid

   #101769

   Purpose

   Mammalian expression of TRAIP fusion protein

   Depositor

   Michael Huen

   Article

   Feng et al Cell Discov. 2016 Jun 28;2:16016. doi: 1

   Insert

   TRAIP / RNF206 (TRAIP Human)
   

   Use

   Tags

   S peptide-flag-streptavidin binding peptide (SFB)

   Expression

   Mammalian

   Mutation

   siRNA-resistant allele

   Promoter

   Available since

   Oct. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLJM60 mUlk1 (wt) barcoded

   Plasmid

   #48799

   Purpose

   expresses wt Ulk1

   Depositor

   David Sabatini

   Article

   Kang et al Science. 2013 Jul 26;341(6144):1236566.

   Insert

   ULK1 (Ulk1 Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG and barcode: GGATCC

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 2, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pEM705 Gso (Ser)

   Plasmid

   #109353

   Purpose

   GsX chimera with Gαo C-terminus (Pertussis toxin insensitive mutant)

   Depositor

   Robert Lucas

   Article

   Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

   Insert

   Gso chimera
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   Changed Cysteine at position 391 to Serine (Pertu…

   Promoter

   CAG

   Available since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO human ULK2 shRNA 91

   Plasmid

   #27634

   Depositor

   Reuben Shaw

   Article

   Egan et al Science. 2010 Dec 23. ():.

   Insert

   human ULK2 shRNA 91
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pCW57.1-3xFLAG-NLS-GFP

   Plasmid

   #198238

   Purpose

   Lentivector with tet-inducible 3xFLAG-NLS-GFP

   Depositor

   Stephen Tapscott

   Article

   Nip et al Hum Mol Genet. 2023 Feb 2:ddad021. doi:

   Insert

   3xFLAG-NLS-GFP
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. BII-Sh-PnGW

   Plasmid

   #133359

   Purpose

   Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   GFP-NLS
   

   Use

   Tags

   HA-tagged GFP

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMSCV PIG IMP-1-short

   Plasmid

   #21659

   Depositor

   David Bartel

   Article

   Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

   Insert

   IGF-II mRNA-binding protein 1 (IGF2BP1 Human)
   

   Use

   Retroviral

   Tags

   GFP and IRES

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti-TK-Firefly-Control

    Plasmid

    #122277

    Purpose

    Lentiviral vector for stable expression of Firefly luciferase.

    Depositor

    Demián Cazalla

    Article

    Gorbea et al Elife. 2019 Sep 20;8. pii: 50530. doi: 1

    Insert

    Firefly luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Thymidine kinase

    Available since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. InterCatch-1xL

    Plasmid

    #186907

    Purpose

    Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR through a single GGGGS linker and the other side expressing EGFP and puromycin resistance gene

    Depositor

    Taekjip Ha, Allen Liu

    Article

    Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

    Insert

    TfR-(GGGGS)1-SpyCatcher003-sfCherry1-10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMRXIP GFP-VAMP8

    Plasmid

    #45919

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

    Insert

    vesicle-associated membrane protein 8 (VAMP8 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

13. p663-UBC-TurboID-V5-GSK3B_IDG-K

    Plasmid

    #158164

    Purpose

    Gateway destination clone of GSK3B (human) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    TurboID-V5-GSK3B (GSK3B Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    TurboID-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB_TRE_Z17-PDGFR_EF1a_mCherry

    Plasmid

    #186303

    Purpose

    Plasmid for inducible mammalian cell expression of the Z17 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction.

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    Z17-PDGFR
    mCherry
    

    Use

    Tags

    HA, myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLX307 hcRed1

    Plasmid

    #117732

    Purpose

    Open reading frame vector encoding hcRed1

    Depositor

    William Hahn

    Article

    Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

    Insert

    hcRed1
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Orai1-myc E106Q MO70

    Plasmid

    #22754

    Depositor

    Anjana Rao

    Article

    Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

    Insert

    Orai1 E106Q (ORAI1 Human)
    

    Use

    Retroviral

    Tags

    myc

    Expression

    Mammalian

    Mutation

    E106Q

    Promoter

    Available since

    Jan. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLJM60-S6K1(wt) barcoded

    Plasmid

    #48801

    Purpose

    expresses wt S6K1

    Depositor

    David Sabatini

    Article

    Kang et al Science. 2013 Jul 26;341(6144):1236566.

    Insert

    S6K1 (Rps6kb1 Rat)
    

    Use

    Lentiviral

    Tags

    FLAG and barcode: GGATCC

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

18. p6642 MSCV-P C-FlagHA 6bE7-Kozak

    Plasmid

    #35020

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    HPV6b E7 (E7 HPV 6b)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR

    Available since

    March 6, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pMRXIP GFP-VAMP7

    Plasmid

    #45920

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

    Insert

    vesicle-associated membrane protein 7 (Vamp7 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pPB-cT3G-cERP2-MEIOC

    Plasmid

    #222562

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of MEIOC

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    MEIOC (C17orf104 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAIP

    Plasmid

    #74171

    Purpose

    Lentiviral vector for expression of heterologous genes

    Depositor

    Jeremy Luban

    Article

    Pertel et al Nature. 2011 Apr 21;472(7343):361-5. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    May 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBS-PtH2B

    Plasmid

    #133390

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    No promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Twist-siRNA3

    Plasmid

    #1784

    Depositor

    Bob Weinberg

    Article

    Yang et al Cell 2004 Jun 25;117(7):927-39.

    Insert

    siTwist (Twist1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 28, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. GG-EBNA-OCT4

    Plasmid

    #69537

    Purpose

    Episomal plasmid encoding 5 gRNAS targeting human OCT4 promoter

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

    Insert

    5 concatenated gRNA transcriptional cassettes targeting OCT4
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p6641 MSCV-P C-FlagHA 18E7-Kozak

    Plasmid

    #35019

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    HPV18 E7 (E7 HPV 18)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR

    Available since

    March 14, 2012

    Availability

    Academic Institutions and Nonprofits only

26. Pinco-mNrp1

    Plasmid

    #21937

    Depositor

    Guido Serini

    Article

    Valdembri et al PLoS Biol. 2009 Jan 27. 7(1):e25.

    Insert

    neuropilin 1 (Nrp1 Mouse)
    

    Use

    Retroviral

    Tags

    HA and His

    Expression

    Mutation

    Promoter

    Available since

    Oct. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

27. pPB-cT3G-cERP2-DMC1

    Plasmid

    #222541

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of DMC1

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    DMC1 (DMC1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p667-UBC-ALPK2-V5-miniTurbo_IDG-K

    Plasmid

    #198009

    Purpose

    Gateway destination clone of ALPK2 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    ALPK2-V5-miniTurbo (ALPK2 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. dCas12j2 KRAB

    Plasmid

    #188508

    Purpose

    Expresses FLAG tagged dCas12j2 KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12j2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D394A

    Promoter

    EF1a

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p667-UBC-AAK1-V5_IDG-K

    Plasmid

    #135257

    Purpose

    Gateway destination clone of AAK1 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    AAK1-V5 (AAK1 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLENTI_RPS10

    Plasmid

    #127129

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    RPS10 (RPS10 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    K24R- please see depositor comments

    Promoter

    Available since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLentiCRISPRv2-TRAF6-targeting sgRNA 2

    Plasmid

    #131346

    Purpose

    gRNA targeting mouse TRAF6

    Depositor

    Jonathan Kagan

    Article

    Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

    Insert

    gRNA targeting mouse TRAF6 (Traf6 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCDH-SIRT6-H133Y

    Plasmid

    #102327

    Purpose

    Human SIRT6 H133Y for mammalian expression

    Depositor

    Hening Lin

    Article

    Zhang et al Nat Chem Biol. 2016 Aug;12(8):614-20. do

    Insert

    SIRT6 (SIRT6 Human)
    

    Use

    Lentiviral

    Tags

    None

    Expression

    Mammalian

    Mutation

    changed histidine 133 to tyrosine

    Promoter

    CMV

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. MSCV PIG Myb

    Plasmid

    #66988

    Purpose

    Retroviral expression plasmid for mouse Myb expression; containing bicistronic IRES-GFP

    Depositor

    Christopher Vakoc

    Article

    Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

    Insert

    Myb (Myb Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    LTR

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMB22

    Plasmid

    #122244

    Purpose

    Lentivirus delivery for stable expression of AsCas12a-VPR

    Depositor

    Darjus Tschaharganeh

    Article

    Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

    Insert

    mammalian codon-optimized Acidaminococcus sp. Cas12a fused to VPR activator
    

    Use

    Lentiviral

    Tags

    NLS and VPR activator

    Expression

    Mutation

    Promoter

    EFs

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMSCV-Pidd1(T788A)

    Plasmid

    #60529

    Purpose

    Retroviral expression of Pidd1 T788A phosphorylation mutant

    Depositor

    Trudy Oliver

    Article

    Terry et al Cell Death Differ. 2014 Oct 10. doi: 10.

    Insert

    Pidd1 (PIDD1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Threonine 788 to Alanine*

    Promoter

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

37. p667-UBC-CDK12-V5-TurboID_IDG-K

    Plasmid

    #135244

    Purpose

    Gateway destination clone of CDK12 (human) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    CDK12-V5-TurboID (CDK12 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-TurboID

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLPC flag-mRAP1 I312R

    Plasmid

    #73138

    Purpose

    Expression of Flag-tagged mRAP1 with single point mutatoin I312R

    Depositor

    Agnel Sfeir

    Article

    Yeung et al Cell Rep. 2013 Jun 27;3(6):1847-56. doi:

    Insert

    Rap1 (Terf2ip Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

39. pMK1221

    Plasmid

    #84220

    Purpose

    Lentiviral expression of shRNAs, compatible with double-shRNA expression, SFFV promoter, mCherry

    Depositor

    Jonathan Weissman, Martin Kampmann

    Article

    Kampmann et al Proc Natl Acad Sci U S A. 2015 Jun 30;11

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pHAGE- TRE-DEST-NBioTAP

    Plasmid

    #53568

    Purpose

    Lentiviral destination vector for N -Bio-1xproteinA constructs using Gateway recombination System. Design for cDNA clones from the CCSBHuman Orfeome Collection.

    Depositor

    Mitzi Kuroda

    Article

    Alekseyenko et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge