174,131 results

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FUW-tetO-SOX9

Plasmid

#141404

Purpose

Inducible Expression of SOX9

Depositor

Lorenz Studer

Article

Tchieu et al Nat Biotechnol. 2019 Mar;37(3):267-275.

Insert

SOX9 (SOX9 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-GFP

Plasmid

#160697

Purpose

Expresses GFP in mammalian cells

Depositor

Christine Mayr

Article

Lee et al Mol Cell. 2019 May 16;74(4):701-712.e9.

Insert

GFP

Expression

Mammalian

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pC1-HyPerRed-mito

Plasmid

#60247

Purpose

Mitochondria-targeted genetically encoded hydrogen peroxide indicator with red fluorescence.

Depositor

Vsevolod Belousov

Article

Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

Insert

HyPerRed-mito

Tags

Mitochondrial targeting signal

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-sumo-NSP12 (SARS-CoV-2)

Plasmid

#159107

Purpose

The SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).

Depositor

Thomas Tuschl

Article

Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

Insert

NSP12

Tags

His-SUMO tag

Expression

Bacterial

Mutation

None

Promoter

T7

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pPP085

Plasmid

#158795

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-2

Tags

6xHis

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-FOXA2

Plasmid

#153110

Purpose

Expression of Flag tagged human FOXA2 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXA2 (FOXA2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCX-SpiCee

Plasmid

#140836

Purpose

mammalian expression of SpiCee, a buffer of calcium that prevents downstream signaling

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

Insert

SpiCee

Tags

mRFP

Expression

Mammalian

Promoter

CAG

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP9

Plasmid

#141263

Purpose

Gateway-compatible Entry vector, with insert of NSP9 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP9 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCRISPR-HOT-P2A-Clover-BlastR

Plasmid

#138568

Purpose

Universal Cleavable Donor Plasmid for tagging with P2A-Clover-BlastR using NHEJ

Depositor

Hans Clevers

Article

Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

Insert

P2A-clover-BlastR

Use

CRISPR

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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SaABE8e

Plasmid

#138500

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e)-nSaCas9

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pFastBac-Dual-M2

Plasmid

#135584

Purpose

High eukaryotic dual protein expression shuttle vector

Depositor

Xiao-Wen Cheng

Article

Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

Type

Empty backbone

Use

Transfer vector

Expression

Insect

Promoter

polyhedrin of AcMNPV

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCDH-cGAS-E225A D227A-mCherry2-EF1-Puro

Plasmid

#132771

Purpose

Interacts with cytoplasmic DNA. Useful for identifying sites of nuclear envelope rupture.

Depositor

Jan Lammerding

Article

Denais et al Science. 2016 Apr 15;352(6283):353-8. do

Insert

Cyclic GMP-AMP synthase (CGAS Human)

Use

Lentiviral

Tags

mCherry2

Expression

Mammalian

Mutation

E225A D227A

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR_L1-YFP-Turbo-L2

Plasmid

#127350

Purpose

gateway entry vector for making N-terminal YFP-TurboID fusions with nuclear and non-nuclear proteins

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

TurboID (BirA mutant)

Use

Gateway Cloning

Tags

GS linker, V5, and YFP

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-F30-Pepper(TAR Variant-2)

Plasmid

#129405

Purpose

circular Pepper RNA

Depositor

Samie Jaffrey

Article

Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

Insert

circular F30-Pepper(TAR Variant-2)

Expression

Mammalian

Promoter

U6

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pQE30-His-PheRS

Plasmid

#124116

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Phenylalanyl-tRNA ligase (pheS, pheT Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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FU-H2B-GFP-IRES-Puro

Plasmid

#69550

Purpose

Lentiviral, selectable fluorescent GFP tagging of nuclei.

Depositor

Charles Gersbach

Insert

Histone 2B - green fluorescent protein fusion

Use

Lentiviral

Tags

GFP fusion

Available Since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-SerRS-His

Plasmid

#124118

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Seryl-tRNA ligase (serS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-LysRS-His

Plasmid

#124114

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Lysine-tRNA ligase (lysU Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-KRAS

Plasmid

#116755

Purpose

Lentiviral expression of KRAS

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

KRAS (KRAS Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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ARHGEF11(DHPH)-CRY2-mCherry

Plasmid

#89481

Purpose

Also known as optoGEF-RhoA, encodes for a fusion between CRY2(1-498)-mCherry and the DHPH domain of ARHGEF11

Depositor

Xavier Trepat Guixer

Article

Valon et al Nat Commun. 2017 Feb 10;8:14396. doi: 10

Insert

ARHGEF11(DHPH) - CRY2(1-498) - mCherry (CRY2, ARHGEF11 Human, Mustard Weed)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-H2B tdTomato

Plasmid

#116870

Purpose

Nuclear localized tdTomato

Depositor

Loren Looger

Article

Economo et al Nature. 2018 Nov;563(7729):79-84. doi: 1

Has Service

AAV Retrograde

Insert

H2B tdTomato

Use

AAV

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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SMARCA2-GFP

Plasmid

#65390

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

SMARCA2 (SMARCA2 Human)

Tags

EmGFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-hA3A-BE3

Plasmid

#113410

Purpose

Expresses hA3A-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3A-BE3 (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.3 LRP5

Plasmid

#115907

Purpose

Expresses human LRP5 in mammalian cells.

Depositor

Harald Junge

Article

Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

Insert

LRP5 (LRP5 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pR_LG lenti-(empty)-del-CTS

Plasmid

#112895

Purpose

Non-integrating lentiviral co-packaging plasmid designed to reduce multiple integrations and recombination during transduction of nucleic acid libraries

Depositor

Paul Blainey

Article

Feldman et al bioRxiv 262121

Type

Empty backbone

Use

Lentiviral

Promoter

none

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EWB-DIO-cyan pre-eGRASP(p32)

Plasmid

#111589

Purpose

An AAV vector that expresses double floxed cyan pre-eGRASP(p32) under the Ef1a promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

cyan pre-eGRASP(p32)

Use

AAV

Promoter

Ef1a

Available Since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pKAM-BFP-Puro

Plasmid

#101864

Purpose

Lentiviral empty backbone, dual BFP and puro markers

Depositor

Archibald Perkins

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

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sgCtr- LentiCRISPRv2

Plasmid

#107402

Purpose

Lentiviral expression of Cas9 and a control gRNA

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

control gRNA

Use

Lentiviral

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-RILP

Plasmid

#110498

Purpose

Fluorescent RILP

Depositor

Terence Dermody, Bernardo Mainou

Article

Mainou et al J Virol. 2012 Aug;86(16):8346-58. doi: 1

Insert

RILP (RILP Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pTol2-hsp70l:Cas9-t2A-GFP, 5xU6:sgRNA

Plasmid

#108871

Purpose

Expression of heat shock inducible Cas9-GFP and U6-driven 5 individual gRNAs for scGESTALT in zebrafish

Depositor

Alex Schier

Article

Raj et al Nat Biotechnol. 2018 Mar 28. pii: nbt.41

Inserts

5xU6:sgRNA
Cas9-t2A-GFP

Use

CRISPR

Promoter

U6 and hsp70

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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