We narrowed to 12,952 results for: sequence

1. pCMV-NES-CRY2PHR-TevC

   Plasmid

   #89877

   Purpose

   expresses NES-CRY2PHR-TevC of BLITz system

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Methods. 2017 May;14(5):495-503. doi

   Insert

   NES-CRY2PHR-TevC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-Lyn-RAB-EKARev

   Plasmid

   #118477

   Purpose

   ddRFP-based single-color ERK biosensor targeted to the plasma membrane.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   Lyn-RAB-EKARev
   

   Use

   Tags

   N-terminal targeting sequence from Lyn-kinase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMDC123SB-AtTAS1c-B/c

   Plasmid

   #51772

   Purpose

   Plant expression vector (2x35S) for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor

   Depositor

   James Carrington

   Article

   Carbonell et al Plant Physiol. 2014 Mar 19.

   Insert

   AtTAS1c-B/c
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   A. thaliana TAS1c precursor sequence including a …

   Promoter

   Available Since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEF-pIgH-ESCargo*

   Plasmid

   #140159

   Purpose

   Expresses a regulatable secretory cargo (without an ER export signal) for mammalian cells

   Depositor

   Benjamin Glick

   Article

   Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

   Insert

   piGH-DsRed-Express2-FKBP(LV)(C22V)
   

   Use

   Tags

   ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pShHELIX(Cas12k-TniQ)-sgRNA_entry (CJT111)

   Plasmid

   #181790

   Purpose

   Expresses 3-component ShHELIX containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   nAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-Cys-ELP(120nm)-LPETGG

   Plasmid

   #90472

   Purpose

   Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGG

   Depositor

   Hermann Gaub

   Article

   Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

   Insert

   Cys-ELP(120nm)-LPETGG
   

   Use

   Tags

   Cysteine and Sortase-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pShCAST(Cas12k-TnsC)-sgRNA_entry (CJT28)

   Plasmid

   #181789

   Purpose

   Expresses 3-component ShCAST containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   ShTnsB, ShTniQ, ShCas12k-ShTnsC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYL322d1/eGFP-C

   Plasmid

   #183152

   Purpose

   Plant transient expression vector containing eGFP tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of eGFP.

   Depositor

   Yao-Guang Liu, Qinlong Zhu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBTK229

   Plasmid

   #110591

   Purpose

   BTK Type 3 coding sequence for golden gate assembly and homologous recombination

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   Kanamycin resistance
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 20, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pGG-NES-TurboID-GFP

    Plasmid

    #209401

    Purpose

    Transiently expressing and visualizing the subcellular localization of TurboID fused with a nuclear export signal (NES) in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Insert

    NES-3XHA-TurboID
    

    Use

    Tags

    GFP

    Expression

    Plant

    Mutation

    TurboID gene sequence C249A

    Promoter

    Cauliflower mosaic virus (CaMV) 35S promoter

    Available Since

    Jan. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-GGG-HIS-CBM-Xmod-DocIII

    Plasmid

    #91698

    Purpose

    CBM-Xmod-DocIII handle with N-Terminal Sortase Recognition Sequence GGG for posttranslational coupling to a C-Terminal LPETGG

    Depositor

    Hermann Gaub

    Article

    Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

    Insert

    GGG-HIS-CBM-Xmod-DocIII
    

    Use

    Tags

    HIS-Tag and Sortase-Tag

    Expression

    Bacterial

    Mutation

    Cysteine deletion in CBM C63S

    Promoter

    T7

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-CohIII-CBM-HIS-LPETGG

    Plasmid

    #91697

    Purpose

    CohIII-CBM handle with C-Terminal Sortase Recognition Sequence LPETGG for Posttranslational Coupling to a N-terminal GGG

    Depositor

    Hermann Gaub

    Article

    Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

    Insert

    CohIII-CBM-HIS-LPETGG
    

    Use

    Tags

    HIS-Tag and Sortase-Tag

    Expression

    Bacterial

    Mutation

    Cysteine deletion in CBM C63S

    Promoter

    T7

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEMI91

    Plasmid

    #74888

    Purpose

    Shuffled 9I27 polyprotein with shuffled codons to allow facile sequencing and unique RE for the 9 cassettes for versatile cloning. See Depositor Comments for more information

    Depositor

    Piotr Marszalek

    Article

    Scholl et al Biomacromolecules. 2016 Jun 8.

    Insert

    Titin I27 x 9 (TTN Human)
    

    Use

    Tags

    His-tag and Strep-tag II

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDH_SBP-mCherry-Ecadherin

    Plasmid

    #65291

    Purpose

    Ecadherin reporter only (no hook) (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Ecadherin
    

    Use

    Lentiviral

    Tags

    IL-2 Signal Sequence and Streptavidin Binding Pro…

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET21c(+)-EFG1mt

    Plasmid

    #31076

    Depositor

    Linda Spremulli

    Article

    Bhargava et al Protein Expr Purif. 2004 Oct . 37(2):368

    Insert

    Mature form human mitochondrial elongation factor G isoform 1 (GFM1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Includes the coding sequence for the mature form.…

    Promoter

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pEF-pIgH-ESCargo(FTV,Orange)

    Plasmid

    #140161

    Purpose

    Expresses a regulatable secretory cargo for mammalian cells

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    piGH-FTVNTT-E2-Orange-FKBP(LV)(C22V)
    

    Use

    Tags

    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21c(+)-mtPheRS

    Plasmid

    #31080

    Depositor

    Linda Spremulli

    Article

    Bullard et al J Mol Biol. 1999 May 14. 288(4):567-77.

    Insert

    Mature form human mitochondrial phenylalanine-tRNA synthetase (FARS2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Includes coding sequence for the mature form. Nt …

    Promoter

    T7

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

18. AID-mTagBFP2-loxP_myo2_neoR-loxP

    Plasmid

    #194055

    Purpose

    Dual-selection cassette plasmid for knocking-in AID-mTagBFP2 into the C. elegans genome

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

    Insert

    IAA17 (AXR3 Mustard Weed)
    

    Use

    Tags

    codon optimized mTagBFP2

    Expression

    Worm

    Mutation

    Promoter

    None

    Available Since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. EC71173

    Plasmid

    #154061

    Purpose

    Level 1, Position 2 Golden Gate vector. HspHv17::Cre-U5-Cre

    Depositor

    Cristobal Uauy

    Article

    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

    Insert

    Cre recombinase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Introduction of U5 intron sequences, to prevent p…

    Promoter

    HvHSP17

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. C1-MPAct(delta1-6)-mRuby3

    Plasmid

    #155223

    Purpose

    F-actin bidning mutant of MPAct

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    Mutated version of F-tractin (aa 15-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Deletion of first 6 AA from F-tractin (9-14 from …

    Promoter

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pENTR-AtTAS1c-B/c

    Plasmid

    #51774

    Purpose

    Gateway-compatible entry vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor

    Depositor

    James Carrington

    Article

    Carbonell et al Plant Physiol. 2014 Mar 19.

    Insert

    AtTAS1c-B/c
    

    Use

    Gateway-compatible entry clone

    Tags

    Expression

    Mutation

    A. thaliana TAS1c precursor sequence including a …

    Promoter

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pShCAST(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT12)

    Plasmid

    #181788

    Purpose

    Expresses 3-component ShCAST containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28-MRPP1

    Plasmid

    #67862

    Purpose

    bacterial expression of TRMT10C, full coding sequence (mitoch. form) + N-term. His tag (thrombin site)

    Depositor

    Walter Rossmanith

    Article

    Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

    Insert

    MRPP1 (TRMT10C Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    mitoch. form (contains aa 40-403)

    Promoter

    T7

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. GOLPH3 shRNA

    Plasmid

    #163860

    Purpose

    shRNA targeting the coding sequences of GOLPH3

    Depositor

    Lei Lu

    Article

    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    golgi phosphoprotein 3 (GOLPH3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDS277

    Plasmid

    #34973

    Purpose

    Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(V529N mutation).

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    Mid2(SS/TM)-GFP-LOVpep
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    V529N

    Promoter

    Available Since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLP-mScarlet-I

    Plasmid

    #209993

    Purpose

    Contains Level 0 Part: Coding sequences (mScarlet-I) for the construction of Level 1 plasmids

    Depositor

    Georg Fritz, Thorsten Mascher

    Article

    Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

    Insert

    mScarlet-I
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGFP FAT

    Plasmid

    #50517

    Purpose

    This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

    Depositor

    Kenneth Yamada

    Article

    Gu et al J Cell Biol. 1999 Jul 26;146(2):389-403.

    Insert

    protein tyrosine kinase, domain
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    truncated (NT2630-3268)

    Promoter

    CMV

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

28. pDONR P2R-P3-ECFP

    Plasmid

    #48353

    Purpose

    Contributes the coding sequence for ECFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

    Depositor

    Anna Planas, Tomas Santalucia

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    ECFP
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Contains a stop codon.

    Promoter

    none

    Available Since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pUB-sSypHer (RbcS-SypHer)

    Plasmid

    #84732

    Purpose

    Expresses SypHer in plant cells-biosensor for pH targeted to chloroplasts stoma

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

    Insert

    sSypHer
    

    Use

    Tags

    RbcS targeting sequence

    Expression

    Plant

    Mutation

    Promoter

    ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

    Available Since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAG-nDsRedFL-miR9/9*/124

    Plasmid

    #177807

    Purpose

    The pre-miR-9/9* and pre-miR-124 sequences have been inserted into the EcoRV of pCAG-nDsRedFL-intron.

    Depositor

    Rikako Sanuki

    Article

    Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

    Insert

    miR-9/9*/124
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge