8,969 results

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pcDNA3-ExRai-AktAR

Plasmid

#118462

Purpose

cpGFP-based excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-AktAR

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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Bmi1-overexpression

Plasmid

#21577

Depositor

Sally Temple

Article

Fasano et al Cell Stem Cell. 2007 Jun 7. 1(1):87-99.

Insert

Bmi-1 (Bmi1 Mouse)

Use

Lentiviral

Tags

ires-eGFP

Expression

Mammalian

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3/HA-RBP2

Plasmid

#14799

Depositor

William Kaelin

Article

Klose et al Cell. 2007 Mar 9. 128(5):889-900.

Insert

Retinoblastoma Binding Protein 2 (KDM5A Human)

Tags

HA

Expression

Mammalian

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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EF.hICN1.Ubc.GFP

Plasmid

#17626

Depositor

Linzhao Cheng

Article

Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

Insert

ICN1 (NOTCH1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pENTR4-CLIPf (w877-2)

Plasmid

#29650

Depositor

Eric Campeau

Type

Empty backbone

Use

Gateway Cloning

Tags

CLIPf

Available Since

July 6, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Fon-oScarlet

Plasmid

#137136

Purpose

Intersectional viral expression of oScarlet in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-oScarlet

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

E95D

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pSV Sport SREBP-1c

Plasmid

#8883

Depositor

Bruce Spiegelman

Article

Tontonoz et al Mol Cell Biol. 1993 Aug . 13(8):4753-9.

Insert

SREBP-1c (Srebf1 Mouse)

Expression

Mammalian

Mutation

Active version of SREBP-1c.

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

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pCGN-ATF6

Plasmid

#11974

Purpose

For mammalian expression of human ATF6 with an HA tag

Depositor

Ron Prywes

Article

Zhu et al Mol Cell Biol. 1997 Sep . 17(9):4957-66.

Insert

ATF6 (ATF6 Human)

Tags

HA

Expression

Mammalian

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-GFAP-hChR2(H134R)-EYFP

Plasmid

#27054

Purpose

AAV expression of humanized ChR2(H134R) fused to EYFP, for optogenetic excitation

Depositor

Karl Deisseroth

Insert

hChR2(H134R)

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

H134R

Promoter

GFAP

Available Since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2A

Plasmid

#15780

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2A (UBE2A Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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PGKdtabpA

Plasmid

#13440

Depositor

Philippe Soriano

Article

Soriano et al Development. 1997 Jul . 124(14):2691-700

Insert

PGKdtabpA

Expression

Mammalian

Available Since

Nov. 16, 2006

Availability

Academic Institutions and Nonprofits only

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AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato

Plasmid

#51082

Purpose

Cre dependent expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore

Depositor

Jonathan Ting

Has Service

AAV1

Insert

GCaMP6s

Use

AAV

Tags

P2A-nls-dTomato

Expression

Mammalian

Promoter

hEF1a

Available Since

Feb. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pAC-QF

Plasmid

#24338

Depositor

Liqun Luo

Article

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

QF

Expression

Insect

Available Since

March 31, 2010

Availability

Academic Institutions and Nonprofits only

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pSXneo 135(T2AG3)

Plasmid

#12402

Depositor

Titia de Lange

Article

Hanish et al Proc Natl Acad Sci U S A. 1994 Sep 13. 9

Insert

135(T2AG3)

Expression

Mammalian

Available Since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only

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pHes1(467)-luc

Plasmid

#41723

Depositor

Ryoichiro Kageyama, Raphael Kopan

Article

Nishimura et al Genomics. 1998 Apr 1;49(1):69-75.

Insert

Hes1 Promoter (-467 to +46) (Hes1 Mouse)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Contains the murine Hes1 promoter (-467 to +46)

Promoter

Hes1 promoter fragment (-467 to +46)

Available Since

March 20, 2013

Availability

Academic Institutions and Nonprofits only

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FBP17-pmCherryC1

Plasmid

#27688

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

FBP17 (FNBP1 Human)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only
pET mOrange LIC cloning vector (u-mOrange)

Plasmid

#29770

Depositor

Scott Gradia

Type

Empty backbone

Tags

mOrange

Expression

Bacterial

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO-GCaMP6f

Plasmid

#128315

Purpose

Expresses GCaMP6f under control of eukaryotic Ef1a promoter after Flp-dependent recombination.

Depositor

Patricia Jensen

Article

Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

Has Service

AAV1

Insert

GCaMP6f

Use

AAV

Tags

His Tag

Expression

Mammalian

Promoter

Ef1a

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac His6 MBP N10 TEV LIC cloning vector (4C)

Plasmid

#30116

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6-MBP-N10-TEV

Expression

Insect

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-human lyn–GFP

Plasmid

#35958

Purpose

Expresses human lyn protein codon optimized for zebrafish expression.

Depositor

Anna Huttenlocher

Article

Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

Insert

LYN (LYN Human)

Tags

EGFP

Expression

Mammalian

Mutation

wt

Promoter

cmv

Available Since

April 27, 2012

Availability

Academic Institutions and Nonprofits only

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V250 pCEP-4HA B56epsilon

Plasmid

#14537

Depositor

David Virshup

Article

Seeling et al Science. 1999 Mar 26. 283(5410):2089-91.

Insert

PPP2R5C B56 epsilon regulatory subunit of PP2A (PPP2R5E Human)

Tags

4 HA

Expression

Mammalian

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-GRAB_DA1m

Plasmid

#113049

Purpose

express the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1m in neurons

Depositor

Yulong Li

Article

Sun et al Cell. 2018 Jul 12;174(2):481-496.e19. do

Has Service

AAV9

Insert

GPCR activation based DA sensor GRAB_DA1m (DRD2 Human)

Use

AAV

Promoter

hSyn

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pET21b-Casp1-His

Plasmid

#11809

Depositor

Guy Salvesen

Insert

Caspase-1 (CASP1 Human)

Tags

His

Expression

Bacterial

Available Since

May 12, 2006

Availability

Academic Institutions and Nonprofits only

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pX330A_D10A-1x2

Plasmid

#58772

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin- soCoChR-GFP

Plasmid

#107708

Purpose

A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Human Synapsin (hSynapsin) promoter

Depositor

Edward Boyden

Article

Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

Has Service

AAV9

Insert

soCoChR-GFP

Use

AAV

Tags

EGFP and KA2

Expression

Mammalian

Promoter

hSynapsin

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pAlbumin

Plasmid

#22037

Depositor

Didier Trono

Insert

partial sequence of human albumine

Use

Lentiviral

Available Since

Dec. 4, 2009

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP13

Plasmid

#141266

Purpose

Gateway-compatible Entry vector, with insert of NSP13 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP13 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pPUR-mU6-sgRNA-Sirius-8XPP7

Plasmid

#121943

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

sgRNA-Sirius-8XPP7

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

mouse U6 promoter

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pSG5-His-SUMO1

Plasmid

#17271

Depositor

Stephen Goff

Article

Yueh et al J Virol. 2006 Jan . 80(1):342-52.

Insert

SUMO1 (SUMO1 Human)

Tags

6xHis

Expression

Mammalian

Available Since

March 1, 2010

Availability

Academic Institutions and Nonprofits only
pCDF1-PTPRD-WT

Plasmid

#25642

Depositor

Todd Waldman

Article

Solomon et al Cancer Res. 2008 Dec 15. 68(24):10300-6.

Insert

Protein tyrosine phosphatase receptor-type delta (PTPRD Human)

Use

Lentiviral

Expression

Mammalian

Mutation

An NsiI restriction site was engineered into the …

Available Since

April 21, 2011

Availability

Academic Institutions and Nonprofits only

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8,969 results