4,143 results

1. pAAV-hSyn-hM4D(Gi)-mCherry

   Plasmid

   #50475

   Purpose

   Gi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.

   Depositor

   Bryan Roth

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   hM4D(Gi)-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   human Synapsin 1

   Available since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV.TBG.PI.Cre.rBG

   Plasmid

   #107787

   Purpose

   AAV vector expressing Cre from TBG promoter

   Depositor

   James M. Wilson

   Has Service

   AAV8

   Insert

   Cre
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TBG

   Available since

   May 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. mEos3.2-C1

   Plasmid

   #54550

   Purpose

   Localization: C1 Cloning Vector, Excitation: 507 / 572, Emission: 516 / 580

   Depositor

   Michael Davidson, Tao Xu

   Article

   Zhang et al Nat Methods. 2012 May 13;9(7):727-9. doi

   Type

   Empty backbone

   Use

   Tags

   mEos3.2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pTRE-Tight-MitoTimer

   Plasmid

   #50547

   Purpose

   Expresses the Timer protein targeted to the mitochondria under a Tet-inducible promoter

   Depositor

   Roberta Gottlieb

   Article

   Hernandez et al Autophagy. 2013 Nov 1;9(11):1852-61. doi

   Insert

   MitoTimer
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   F12S mutation in Timer

   Promoter

   Available since

   March 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. ppyCAG_RNaseH1_WKKD

   Plasmid

   #111905

   Purpose

   Express WKKD mutated RNASEH1 in mammalian cell. The combination of three specific mutations (W43A, K59A, K60A) in the binding domain prevents the enzyme from binding to RNA/DNA hybrids

   Depositor

   Xiang-Dong Fu

   Article

   Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

   Insert

   RNASEH1 (RNASEH1 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Changed D 210 to N, W 43 to A, K 59 to A, K 60 to…

   Promoter

   Available since

   July 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. AIO-GFP

   Plasmid

   #74119

   Purpose

   All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and EGFP-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing

   Depositor

   Steve Jackson

   Article

   Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLCAR-CD19-BBz

   Plasmid

   #135992

   Purpose

   Modular 41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

   Depositor

   Scott McComb

   Article

   Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

   Insert

   FMC63-41BB-3z-P2A-EGFP (CD247, CD28, TNFRSF9 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a-short

   Available since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCherry-ZO1-C-14

   Plasmid

   #55165

   Purpose

   Localization: Tight Junctions, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   ZO1 (TJP1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3 Flag beta-1-adrenergic-receptor

   Plasmid

   #14698

   Depositor

   Robert Lefkowitz

   Article

   Tang et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

   Insert

   B1AR (ADRB1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV/D377Y-mPCSK9

    Plasmid

    #58376

    Purpose

    Expresses GoF mutant murine PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

    Depositor

    Jacob Bentzon

    Article

    Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

    Has Service

    AAV8

    Insert

    proprotein convertase subtilisin/kexin type 9 (Pcsk9 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    D377Y

    Promoter

    HCRApoE/hAAT

    Available since

    Aug. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. VAMP2-pHmScarlet

    Plasmid

    #166890

    Purpose

    For vesicle exocytosis visualization using pHmScarlet

    Depositor

    Pingyong Xu

    Article

    Liu et al Nat Commun. 2021 Mar 3;12(1):1413. doi:

    Insert

    VAMP2 (Vamp2 Mouse)
    

    Use

    Tags

    pHmScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lenti-luciferase-P2A-Neo

    Plasmid

    #105621

    Purpose

    3rd generation lentiviral plasmid for expression of luciferase with Neo resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    Luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVpuro-CMV-N-mCherry

    Plasmid

    #123221

    Purpose

    N-terminal mCherry tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVax1-hVEGF165

    Plasmid

    #74466

    Purpose

    expression of hVEGF165 in mammalian cells

    Depositor

    Loree Heller

    Article

    Marshall WG et al Gene Ther. 2010 Mar;17(3):419-23. doi: 1

    Insert

    vascular endothelial growth factor 165 (VEGFA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-FLAG-LKB1

    Plasmid

    #8590

    Depositor

    Lewis Cantley

    Article

    Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

    Insert

    LKB1 (STK11 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3 Flag p38 alpha

    Plasmid

    #20351

    Depositor

    Roger Davis

    Article

    Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

    Insert

    pDCNA3 Flag p38 alpha (Mapk14 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKOV

    Plasmid

    #25769

    Depositor

    George Church

    Article

    Link et al J Bacteriol. 1997 Oct . 179(20):6228-37.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKanCMV-mClover3-mRuby3

    Plasmid

    #74252

    Purpose

    Mammalian expression of tandem fusion of mClover3-mRuby3

    Depositor

    Michael Lin

    Article

    Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

    Insert

    mClover3-mRuby3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lck-GFP

    Plasmid

    #61099

    Purpose

    Expression of a membrane-targeted form of eGFP

    Depositor

    Steven Green

    Article

    Benediktsson et al J Neurosci Methods. 2005 Jan 30;141(1):4

    Insert

    Lck-GFP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pXPR_120

    Plasmid

    #96917

    Purpose

    for CRISPRa, lentiviral expression of dCas9-VPR 2A BlastR

    Depositor

    John Doench, David Root

    Article

    Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    VPR

    Expression

    Mammalian

    Mutation

    D10A, N863A

    Promoter

    EF1a

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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